BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3505.p (308 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthino... 89 3e-19 gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid... 87 9e-19 gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Ja... 87 1e-18 gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthi... 83 2e-17 >gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthinobacterium sp. Marseille] Length = 400 Score = 89.4 bits (220), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 61/232 (26%), Positives = 101/232 (43%), Gaps = 6/232 (2%) Query: 51 EFLAALPRLKVLFSSGAGVDHIDMTVVPPHVAIVRMVEPGIINGMLEYVTMSVLALHRHL 110 E +A +LK++ ++G G DH D+ H V V + + E+V M +L+ R+ Sbjct: 107 ERIAKAKKLKMIVTAGIGSDHTDLEAANKHNITVAEVTYCNSHSVAEHVVMMILSQVRNY 166 Query: 111 FEYQEFQRQGLWQPIEV----YPPSTCSVGVMGLGVLGQAVLERLGVFGYR--RLGWNRS 164 G W + Y SVG + G +G VL L F + + +R Sbjct: 167 IPSYNQVINGGWNIADCVERSYDLEAMSVGTVAAGRIGLRVLRLLKPFDVKLHYMDRHRL 226 Query: 165 PRSLPDVRCFEGEKALNEFLRQCDILICLLPLTDATRGILGKRLFATLPAGSSLLHVGRG 224 P ++ L+ + CD++ PL T ++ ++ GS +++ RG Sbjct: 227 PEAVEKELNLTYHSTLDSLTKVCDVVTLNCPLHPETEHMINEKTLKNFKRGSYIINTARG 286 Query: 225 GHLDQDALLDALDRGQLGRAVLDVCDPEPLPVDHPLWRHPRVVLTPHVASMT 276 D+DA++ AL GQL DV P+P P DHP P +TPH++ + Sbjct: 287 KLCDRDAIVAALKSGQLAGYAGDVWFPQPAPKDHPWRTMPHHGMTPHISGTS 338 >gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 323 Score = 87.4 bits (215), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 67/232 (28%), Positives = 101/232 (43%), Gaps = 12/232 (5%) Query: 51 EFLAALPRLKVLFSSGAGVDHIDMTVVPPH-VAIVRMVEPGIINGMLEYVTMSVLALHRH 109 + L LP LK++ +G HID+ + IV V G E ++A R Sbjct: 62 QLLEKLPNLKLIAQTGKVSGHIDLPAAAARGITIVEGV--GDPTAPAELTWTLIMAAMRK 119 Query: 110 LFEYQEFQRQGLWQPIEVYPPSTC--------SVGVMGLGVLGQAVLERLGVFGYRRLGW 161 L +Y + GLWQ + P ++ + G G +GQ + FG + W Sbjct: 120 LPQYVNNLKDGLWQIASMSPARNTLGTALKGRTLAIWGYGRIGQMIAAYGKAFGMDVMVW 179 Query: 162 NRSPRSLPDVR-CFEGEKALNEFLRQCDILICLLPLTDATRGILGKRLFATLPAGSSLLH 220 R VR + + F + D+L L L DATRGI+ A + + ++ Sbjct: 180 GRPASCEAAVRDGYRAASSQQAFFSEADVLTLHLRLNDATRGIVTADDLARMKPQALFVN 239 Query: 221 VGRGGHLDQDALLDALDRGQLGRAVLDVCDPEPLPVDHPLWRHPRVVLTPHV 272 R + DAL AL++G+ G A LDV + EPLP PL R V+ +PH+ Sbjct: 240 TSRAELVATDALQAALEQGRPGYAALDVFENEPLPPGSPLLRMENVLASPHL 291 >gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 327 Score = 87.0 bits (214), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 67/241 (27%), Positives = 119/241 (49%), Gaps = 14/241 (5%) Query: 47 QVPPEFLAALPRLKVLFSSGAGVDHIDMTVVPPHVAIVRMVEPGIIN-GMLEYVTMSVLA 105 ++ + +AA P+LK + + G ++ID+ +V P ++N +Y ++A Sbjct: 56 KMSADVIAANPQLKAICNVAVGYNNIDVAAATAAGVMVTNT-PDVLNETTADYAWTLLMA 114 Query: 106 LHRHLFEYQEFQRQGLWQ--PIEVYPPST---CSVGVMGLGVLGQAVLERLGVFGYRRLG 160 R + E + + R G W+ + + + ++G++G+G +GQA+ R F + Sbjct: 115 NARRVCESEHWLRAGNWKHWRFDSFLGADIHGATLGILGMGRIGQAIARRSTGFDMNVIY 174 Query: 161 WNRSPRSLPDVRCFEGEK---ALNEFLRQCDILICLLPLTDATRGILGKRLFATLPAGSS 217 NRS R+ P+ + + E LR D LI +LP + T I+G A + ++ Sbjct: 175 HNRS-RATPEQEAYANNARYVSKEELLRSADHLILVLPYSPQTHHIIGAAELALMKPTAT 233 Query: 218 LLHVGRGGHLDQDALLDALDRGQLGRAVLDVCDPEPLPVDHP-LWRHPRVVLTPHVASMT 276 L+++ RGG +D AL+ AL ++ A LDV + EP HP VVLTPH+ S + Sbjct: 234 LVNIARGGIVDDVALIAALRENRIASAGLDVFENEPAL--HPDFLTLSNVVLTPHIGSGS 291 Query: 277 Q 277 + Sbjct: 292 E 292 >gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthinobacterium sp. Marseille] Length = 336 Score = 82.8 bits (203), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 68/247 (27%), Positives = 122/247 (49%), Gaps = 25/247 (10%) Query: 47 QVPPEFLAALPRLKVLFSSGAGVDHIDMTVVPPHVAIVRMVEPGIINGML-----EYVTM 101 V FLAA P+LK++F++ G D+ D+ R V GI+ +L E Sbjct: 56 SVDDSFLAACPKLKIVFAALKGYDNFDVDACTK-----RGVWFGIVPDLLTVPTAELTVG 110 Query: 102 SVLALHRHLFEYQEFQRQGL---WQPIEVYPP--STCSVGVMGLGVLGQAVLERLGVFGY 156 +L L RH+ + R G W+P ++Y + ++G++G+G +G+A+ +RL F Sbjct: 111 LLLGLTRHVMAGDDHVRSGTFHGWRP-KLYGAGLAGSTIGIIGMGRVGKAIAKRLSGFEM 169 Query: 157 RRLGWNRSPRSLPDVRCFEGEK-ALNEFLRQCDILICLLPLTDATRGILGKRLFATLPAG 215 + + P + D + + + + +E L D ++ +LP+T T ++ + + G Sbjct: 170 NAVYCDSVPLNPVDEQAWNARQVSFDELLTCSDFVVPMLPMTSDTFHLIDAHAISKMRRG 229 Query: 216 SSLLHVGRGGHLDQDALLDALDRGQLGRAVLDVCDPE-------PLPVDHPLWRH-PRVV 267 S LL+ RG +D++A+++AL++G L DV + E PL V L + + + Sbjct: 230 SYLLNTSRGSVVDENAVVEALNQGHLAGYAADVFEMEEWARPDRPLTVPQALLNNRTQTL 289 Query: 268 LTPHVAS 274 TPHV S Sbjct: 290 FTPHVGS 296 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.325 0.142 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 755,355 Number of extensions: 30967 Number of successful extensions: 116 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 4 Length of query: 308 Length of database: 1,221,021 Length adjustment: 83 Effective length of query: 225 Effective length of database: 914,170 Effective search space: 205688250 Effective search space used: 205688250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 129 (54.3 bits)