BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3504.p (589 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979865|ref|YP_001352342.1| dihydroxy-acid dehydratase [Jan... 270 2e-73 gi|152980587|ref|YP_001354001.1| phosphogluconate dehydratase [J... 160 2e-40 >gi|152979865|ref|YP_001352342.1| dihydroxy-acid dehydratase [Janthinobacterium sp. Marseille] Length = 557 Score = 270 bits (690), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 194/531 (36%), Positives = 285/531 (53%), Gaps = 24/531 (4%) Query: 31 HRSTLAADGWTREDLMEKPIVGILNTWSDINPCNMNLRMLAQKVREGILEAGGIP--FEI 88 +RS A G+ +ED +KP+VG+ N S I PCN L+ LA + I ++GG P F Sbjct: 19 NRSMYYAMGYEKEDF-DKPMVGVANGHSTITPCNSGLQKLADIAIKTIKDSGGNPQVFGT 77 Query: 89 PL----MSISENLIKPSSFPFRNMLAMDTEECIRGYPLDAVVLMGGCDKTQPGLLMGAAS 144 P MS+ +K S R ++A E + G +D V+++GGCDK PG ++ Sbjct: 78 PTISDGMSMGTEGMK-YSLISREVIADCIETAVNGQWMDGVLVIGGCDKNMPGGMIAMLR 136 Query: 145 ANVPAIALNSGPGTPRSAIGGPLATANGVWRFVDQLRAGCLSTESFENFERQTMGSPGHC 204 NVP+I + G P + G L T + V + AG +S E F+ ER S G C Sbjct: 137 TNVPSIYVYGGTIKPGNWKGKDL-TIVSAFEAVGEFTAGRMSQEDFDGIERNACPSAGSC 195 Query: 205 SEMGTASTMSSISEALGLALPGSSLIPSSDGRRSQIAKAVGRCSVALALSGDLAPANILD 264 M TA+TMSS EALGLALP SS + + D + A R + A+ DL P +I+ Sbjct: 196 GGMYTANTMSSSFEALGLALPYSSTMANPDDEKVGSAAESARVLIE-AIKNDLKPRDIVT 254 Query: 265 ARAFDNAITLLCAVGGSTNAIIHLLALAGRVGVPLTLDRFDEIARRVPLISNVQPAGEHL 324 +A +NA+ ++ A GGSTNA++H LA+A V T+D F+ + ++VP+I +++P+G+++ Sbjct: 255 RKAIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERMRKKVPVICDLKPSGKYV 314 Query: 325 TERLMNAGGVPALLHVL--RPLLHLDARTVDGRTIGE-IADQAEV--SDTDVIRQLDTPV 379 L AGG+P ++ VL LLH D T+ G+T+ E +A V +D VI + + Sbjct: 315 ATDLHKAGGIPQVMKVLLDAGLLHGDCMTITGQTVAEALAHIPSVPRADQHVIHPIKDAL 374 Query: 380 KPGPSLVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLADTIDDPTMNIDANS 439 L ++KGNL+P G V K + G A VF+D + D I I A Sbjct: 375 YEQGHLAILKGNLSPEGCVAKITGLKNPVIT--GPARVFDDEYSAMDAI--MANKIVAGD 430 Query: 440 VLILRNVGPVGA-AMPEWGMLPLPRRLMEHGVKDML-RISDARMSGTAYGTSILHVSPEA 497 VL++R +GP G MPE ML L+ G+ + + I+D R SG +G + HVSPEA Sbjct: 431 VLVMRYLGPKGGPGMPE--MLAPTSALVGQGLGESVGLITDGRFSGGTWGMVVGHVSPEA 488 Query: 498 AVGGPLSLVRTGDIVELDVSERRLELIVSEEILAQRRKETPQHAPSPHVRG 548 VGG + LV GD V +D + ++L V EE +A+RR Q AP + RG Sbjct: 489 FVGGLIGLVEEGDSVTIDAHKLLIQLNVPEEEIARRRANWKQPAPR-YTRG 538 >gi|152980587|ref|YP_001354001.1| phosphogluconate dehydratase [Janthinobacterium sp. Marseille] Length = 642 Score = 160 bits (405), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 157/568 (27%), Positives = 257/568 (45%), Gaps = 53/568 (9%) Query: 9 EKQKPLLRSARWFR-KNDLPGFIHRSTLA----ADGWTREDLMEK--------PIVGILN 55 E+ +P R+A R +N +HRSTL+ A G+ +K P + I++ Sbjct: 16 ERSRPY-RAAYLQRLENTRQKGVHRSTLSCTNLAHGFAAAPQNDKLVLKQQTAPSIAIVS 74 Query: 56 TWSDINPCNMNLRMLAQKVREGILEAGGIP-FE--IPLMSISENLIKPS---SFPFRNML 109 ++D+ + +++ + +AGG+ F +P M +P S R+ + Sbjct: 75 AYNDMLSAHQPFERFPHIIKDAVRKAGGVAQFAGGVPAMCDGITQGQPGMELSLFSRDTI 134 Query: 110 AMDTEECIRGYPLDAVVLMGGCDKTQPGLLMGAAS-ANVPAIALNSGPGTPRSAIGGPLA 168 AM T + DA + +G CDK PGL++GA ++PA+ + +GP T S + P Sbjct: 135 AMSTAIALSHNMFDAALYLGVCDKIVPGLVIGALHFGHLPAVFVPAGPMT--SGMSNPEK 192 Query: 169 TANGVWRFVDQLRA-GCLSTESFENFERQTMGSPGHCSEMGTASTMSSISEALGLALPGS 227 + QL A G + ++ E Q+ G C+ GTA++ + E +GL LPG+ Sbjct: 193 AK------IRQLYAQGKIGRDALLEAEGQSYHDAGTCTFYGTANSNQMLMEVMGLHLPGA 246 Query: 228 SLI-PSSDGRRSQIAKAVGRCSVALALSGDLAP-ANILDARAFDNAITLLCAVGGSTNAI 285 + I P++ R + A A R A P ++D + NA+ L A GGSTN Sbjct: 247 AFITPNTPLRDALTAHAAQRAVEISAQGKSFTPIGRLVDEKTIVNAVVALLATGGSTNHT 306 Query: 286 IHLLALAGRVGVPLTLDRFDEIARRVPLISNVQPAGEHLTERLMNAGGVPALLHVLRP-- 343 +HL+A+A G+ + + FD ++ PL++ + P G+ AGG ++ L Sbjct: 307 LHLVAMAKAAGIVIDWNDFDALSAIAPLVARIYPNGDADVNHFHAAGGTGFVIRELLDAG 366 Query: 344 LLHLDARTVDGRTIGEIADQ-------------AEVS-DTDVIRQLDTPVKPGPSLVVVK 389 LLH D T+ GR + A + A VS +TDV+R P + ++ Sbjct: 367 LLHDDVLTILGRGLRAHAAEPFLQGEQVVWQPAASVSGNTDVLRTAADPFSGDGGMKLLS 426 Query: 390 GNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLADTIDDPTMNIDANSVLILRNVGPV 449 GNL + + + R A+VF+ + +N D + +LR+ GP Sbjct: 427 GNLGRAVVKVSAVKVQHRIV--RAPAIVFDSQEAFMHAYQAGELNRD--FIAVLRHQGPR 482 Query: 450 GAAMPEWGMLPLPRRLMEHGVKDMLRISDARMSGTAYGT-SILHVSPEAAVGGPLSLVRT 508 MPE L ++ + + ++D RMSG + + +HVSPE GGPL VR Sbjct: 483 ANGMPELHALTPALGSLQDAGRHVALVTDGRMSGASGKVPAAIHVSPEVLAGGPLGRVRD 542 Query: 509 GDIVELDVSERRLELIVSEEILAQRRKE 536 GDI+EL+ L+ +V E A R E Sbjct: 543 GDIIELNAETGVLQALVPEAEWAARGVE 570 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,410,837 Number of extensions: 60388 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 2 Length of query: 589 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 501 Effective length of database: 895,685 Effective search space: 448738185 Effective search space used: 448738185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)