BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3384.p (567 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979897|ref|YP_001352888.1| 2-isopropylmalate synthase [Jan... 886 0.0 gi|152980069|ref|YP_001353835.1| 2-isopropylmalate synthase [Jan... 159 5e-40 >gi|152979897|ref|YP_001352888.1| 2-isopropylmalate synthase [Janthinobacterium sp. Marseille] Length = 552 Score = 886 bits (2290), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/553 (76%), Positives = 485/553 (87%), Gaps = 2/553 (0%) Query: 1 MMLSNPAAKYRPFPAIDLPDRTWPSKVISTPPIWMSTDLRDGNQALIEPMNAERKLRFFE 60 MML+NPAAKYR FP + L DRTWP++VISTPPIWMSTDLRDGNQALIEPMN E+KLRFF+ Sbjct: 1 MMLTNPAAKYRAFPPVQLKDRTWPNRVISTPPIWMSTDLRDGNQALIEPMNHEKKLRFFD 60 Query: 61 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSRDELIRRTVESLEG 120 +LLK GLKEIEV FPSASQTDFDFVRKLI E RIPDDVTI VLTQ+R++LIRRTV+S+ G Sbjct: 61 MLLKIGLKEIEVAFPSASQTDFDFVRKLIDEKRIPDDVTIEVLTQAREDLIRRTVDSVVG 120 Query: 121 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF 180 +++AIVH+YN+ APAFR+IVFNMS+EE+K +AV GT+L++ELTDA P TEW F+YSPE+F Sbjct: 121 SRRAIVHMYNATAPAFRRIVFNMSKEEVKALAVAGTKLIRELTDAHPETEWVFQYSPETF 180 Query: 181 STTELDFSKEICDAVCETWGATPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRAST 240 S TELDF+KE+CDAV E W TPERK+I+NLP TVE +TPNVYADQ+EWM RNL R S Sbjct: 181 SMTELDFAKEVCDAVSEAWQPTPERKMIINLPGTVEASTPNVYADQVEWMHRNLARRDSI 240 Query: 241 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 I+SVHPHNDRG AVA+AELAVMAGADR+EGCLFGNGERTGNVDLVTLALNLY+QG+NPGL Sbjct: 241 ILSVHPHNDRGCAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYSQGINPGL 300 Query: 301 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY 360 DFSDID +RQ VEECNQIPVHPRHPYVGDLV+TAFSGSHQDAIKKGFAKQ+PD IWEVPY Sbjct: 301 DFSDIDEIRQCVEECNQIPVHPRHPYVGDLVYTAFSGSHQDAIKKGFAKQKPDEIWEVPY 360 Query: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 +P+DPADLGRSYDAVIRVNSQSGKGGM YLLEQEYGL LPRRLQIEFSRA+QR D TG Sbjct: 361 MPVDPADLGRSYDAVIRVNSQSGKGGMTYLLEQEYGLVLPRRLQIEFSRAVQRITDETGG 420 Query: 421 EIAAADIHAIFQREYLERTEPYVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNG 480 E+AA DIH IF EYL PY YR H + D SK + I+V++ ++G+PV+++G GNG Sbjct: 421 EVAANDIHTIFNSEYLNAVTPYHYRNHHI--DDSKDGEVQIKVELEKDGKPVSMQGVGNG 478 Query: 481 PIDAFVQALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK 540 PIDAF+ ALGL+IKLMDFHEHAIGAGA A AASY+ELR+N T FGVGID NIVTASF+ Sbjct: 479 PIDAFINALGLNIKLMDFHEHAIGAGASANAASYVELRVNNGVTRFGVGIDGNIVTASFR 538 Query: 541 AVLSAVNRQIAIS 553 AVLSAVNR IA++ Sbjct: 539 AVLSAVNRDIALA 551 >gi|152980069|ref|YP_001353835.1| 2-isopropylmalate synthase [Janthinobacterium sp. Marseille] Length = 516 Score = 159 bits (401), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 148/530 (27%), Positives = 246/530 (46%), Gaps = 41/530 (7%) Query: 29 STPPIWMSTDLRDGNQALIEPMNAERKLRFFELLLKTGLKEIEVGFPSASQTDFDFVRKL 88 S I T LRDG Q+ M + K+R L + + IE GF +ASQ DF+ + + Sbjct: 6 SNKLIIFDTTLRDGEQSPGASMTKDEKIRIARQLERLKVDIIEAGFAAASQGDFESIHAI 65 Query: 89 IVENRIPDDVTIIVLTQSRDELIRRTVESLEGAKKAIVHLYNSVAPAFRKIVFNMSREEI 148 + I D T+ L+++ D+ I R E+L A++ +H + + +P + M+ +++ Sbjct: 66 ---SGIIKDATVCSLSRANDKDIARAAEALAPAERKRIHTFLATSPLHMEKKLRMTPDQV 122 Query: 149 KNIAVTGTRLVKELTDARPGTEWRFEYSPESFSTTELDFSKEICDAVCETWGATPERKVI 208 A R + TD E+SPE S +++DF + +AV + GAT Sbjct: 123 FEQAKMAVRYARNFTD-------DIEFSPEDGSRSDIDFLCRVIEAVIKE-GATT----- 169 Query: 209 LNLPSTVECATPNVYADQIEWMCRNLKDRASTIISVHPHNDRGTAVASAELAVM-AGADR 267 +N TV P +Y + ++ + + + + SVH HND G AVA++ VM GA + Sbjct: 170 INFADTVGYGVPGLYGNMLKTLRERIPNSDKAVWSVHCHNDLGMAVANSLAGVMIGGARQ 229 Query: 268 VEGCLFGNGERTGNVDLVTLALNLYTQ----GVNPGLDFSDIDVVRQVVEECNQIPVHPR 323 VE + G GER GN L + + + T+ + G+D + I ++V + V P Sbjct: 230 VECTINGLGERAGNTALEEIVMAVRTRKDHFNLEVGIDTTQIVPTSKLVSQITGFAVQPN 289 Query: 324 HPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSG 383 VG F SG HQD + K Y + D+G + + ++ + SG Sbjct: 290 KSVVGANAFAHASGIHQDGVLKA----------RDTYEIMRAEDVGWTTNKIV-LGKLSG 338 Query: 384 KGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKEIAAADIHAIFQREYLERTEPYV 443 + LE E G+ L +++ + +E +I DI A+ E + Y Sbjct: 339 RNAFKQRLE-ELGITLDSGAEMDAAFVRFKELADRKSDIFDEDIMALVSDEQQSHEKEY- 396 Query: 444 YRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNGPIDAFVQAL----GLDIKLMDFH 499 Y +S+ SS V +G+ + +G GNGP+DA V A+ G +L+ F Sbjct: 397 YSFVSLSQQSSTGSKPAANVVFSIDGKEESCKGEGNGPVDAIVNAIEAKVGSGAELLLFS 456 Query: 500 EHAIGAGADAKAASYIELRLNEAPTGF-GVGIDANIVTASFKAVLSAVNR 548 +AI G +++ + +RL++A GVG D +IV AS KA +SA+N+ Sbjct: 457 VNAITTGTESQGE--VTMRLSKAGRIVNGVGADLDIVVASAKAYISALNK 504 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,255,508 Number of extensions: 51817 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 2 Length of query: 567 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 479 Effective length of database: 895,685 Effective search space: 429033115 Effective search space used: 429033115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)