BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3299.p (319 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Ja... 109 2e-25 gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid... 88 7e-19 gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthino... 83 2e-17 gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthi... 77 1e-15 >gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 327 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 97/317 (30%), Positives = 148/317 (46%), Gaps = 32/317 (10%) Query: 2 KVLITDYDFPDVDLELALYRQAGVEVVTAQCRTEDDVIAASEGCQGLLVQYAPV------ 55 KVL+ FPDV L Q EV + Q ED + + +E Q + + A V Sbjct: 4 KVLVARATFPDVIERL----QQQFEVESNQ---EDKIFSPAELLQKMQGKDAVVVTASEK 56 Query: 56 -NAKVFAARPEIRIASRYGAGFDTINTADAARYGVWVANSPDYGVGEVATHALAMALDLI 114 +A V AA P+++ G++ I+ A A GV V N+PD A +A + + Sbjct: 57 MSADVIAANPQLKAICNVAVGYNNIDVAAATAAGVMVTNTPDVLNETTADYAWTLLMANA 116 Query: 115 RNITVYDRAVKAGQW-HYTTAGII-PRASELTLGIIGLGRIGKRMAHISRNLFKRVIAYD 172 R + + ++AG W H+ + TLGI+G+GRIG+ +A S VI ++ Sbjct: 117 RRVCESEHWLRAGNWKHWRFDSFLGADIHGATLGILGMGRIGQAIARRSTGFDMNVIYHN 176 Query: 173 PYIIDGDFPAYVER---VGREDLFRQAHVVSLHTPLNDETRNMIDASLLALLPPQSYLVN 229 + AY V +E+L R A + L P + +T ++I A+ LAL+ P + LVN Sbjct: 177 RSRATPEQEAYANNARYVSKEELLRSADHLILVLPYSPQTHHIIGAAELALMKPTATLVN 236 Query: 230 SARGGLIQIDDLLAALENGQLKGAALDVLPVEPPTTSAAIVQHP------RVLLSPHAAF 283 ARGG++ L+AAL ++ A LDV EP HP V+L+PH Sbjct: 237 IARGGIVDDVALIAALRENRIASAGLDVFENEPAL-------HPDFLTLSNVVLTPHIGS 289 Query: 284 FSDVAARELRRKAAQNL 300 S+ R + A+ NL Sbjct: 290 GSEKTRRAMADCASANL 306 >gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 323 Score = 87.8 bits (216), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 80/268 (29%), Positives = 124/268 (46%), Gaps = 31/268 (11%) Query: 64 PEIRIASRYGAGFDTINTADAARYGVWVANSPDYGVGEVA-----THALAMALDLIRNIT 118 P +++ ++ G I+ AA G+ + GVG+ T L MA +R + Sbjct: 68 PNLKLIAQTGKVSGHIDLPAAAARGITIVE----GVGDPTAPAELTWTLIMAA--MRKLP 121 Query: 119 VYDRAVKAGQWHYTTAGIIPRASEL-------TLGIIGLGRIGKRMAHISRNLFKRVIAY 171 Y +K G W A + P + L TL I G GRIG+ +A + V+ + Sbjct: 122 QYVNNLKDGLWQ--IASMSPARNTLGTALKGRTLAIWGYGRIGQMIAAYGKAFGMDVMVW 179 Query: 172 ------DPYIIDGDFPAYVERVGREDLFRQAHVVSLHTPLNDETRNMIDASLLALLPPQS 225 + + DG Y ++ F +A V++LH LND TR ++ A LA + PQ+ Sbjct: 180 GRPASCEAAVRDG----YRAASSQQAFFSEADVLTLHLRLNDATRGIVTADDLARMKPQA 235 Query: 226 YLVNSARGGLIQIDDLLAALENGQLKGAALDVLPVEPPTTSAAIVQHPRVLLSPHAAFFS 285 VN++R L+ D L AALE G+ AALDV EP + +++ VL SPH + Sbjct: 236 LFVNTSRAELVATDALQAALEQGRPGYAALDVFENEPLPPGSPLLRMENVLASPHLGYVE 295 Query: 286 DVAARELRRKAAQNLIDWDRNGRPSYVV 313 + A QN++D+ NG P V+ Sbjct: 296 KDSYELYFGSAFQNIVDF-ANGTPKNVL 322 >gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthinobacterium sp. Marseille] Length = 400 Score = 83.2 bits (204), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 76/278 (27%), Positives = 127/278 (45%), Gaps = 22/278 (7%) Query: 53 APVNAKVFAARPEIRIASRYGAGFDTINTADAARYGVWVANSPDYGVGEVATHALAMALD 112 A + A+ A ++++ G G D + A ++ + VA VA H + M L Sbjct: 102 AYMTAERIAKAKKLKMIVTAGIGSDHTDLEAANKHNITVAEVTYCNSHSVAEHVVMMILS 161 Query: 113 LIRN-ITVYDRAVKAGQWHYTTAGIIPRASEL---TLGIIGLGRIGKRMAHISRNLFKRV 168 +RN I Y++ + G + A + R+ +L ++G + GRIG R+ L K Sbjct: 162 QVRNYIPSYNQVINGG---WNIADCVERSYDLEAMSVGTVAAGRIGLRVL----RLLKPF 214 Query: 169 IAYDPYIIDGDFPAYVER-------VGREDLFRQAHVVSLHTPLNDETRNMIDASLLALL 221 Y+ P VE+ + L + VV+L+ PL+ ET +MI+ L Sbjct: 215 DVKLHYMDRHRLPEAVEKELNLTYHSTLDSLTKVCDVVTLNCPLHPETEHMINEKTLKNF 274 Query: 222 PPQSYLVNSARGGLIQIDDLLAALENGQLKGAALDVLPVEPPTTSAAIVQHPRVLLSPHA 281 SY++N+ARG L D ++AAL++GQL G A DV +P P ++PH Sbjct: 275 KRGSYIINTARGKLCDRDAIVAALKSGQLAGYAGDVWFPQPAPKDHPWRTMPHHGMTPHI 334 Query: 282 AFFSDVAARELRRKAAQNLIDWDRNGRP---SYVVVQG 316 + S + A+ + +++ GRP Y++VQG Sbjct: 335 SGTS-LTAQTRYAAGTREILECYFEGRPIRDEYLIVQG 371 >gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthinobacterium sp. Marseille] Length = 336 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 79/325 (24%), Positives = 148/325 (45%), Gaps = 23/325 (7%) Query: 2 KVLITDYDFPDVDLELALYRQAGVEVVTAQCRTEDDVIAASEGCQGLLVQYAP--VNAKV 59 K++IT + P++ +E+ V T + ++++ S+ ++ + P V+ Sbjct: 4 KIVITHWVHPEI-VEMLSSVAEVVTNDTLETLPREELLRRSKDADAVMA-FMPDSVDDSF 61 Query: 60 FAARPEIRIASRYGAGFDTINTADAARYGVWVANSPDYGVGEVATHALAMALDLIRNITV 119 AA P+++I G+D + + GVW PD A + + L L R++ Sbjct: 62 LAACPKLKIVFAALKGYDNFDVDACTKRGVWFGIVPDLLTVPTAELTVGLLLGLTRHVMA 121 Query: 120 YDRAVKAGQWHYTTAGIIPRA-----SELTLGIIGLGRIGKRMAHISRNLFKRVIAYDPY 174 D V++G +H G P+ + T+GIIG+GR+GK +A + D Sbjct: 122 GDDHVRSGTFH----GWRPKLYGAGLAGSTIGIIGMGRVGKAIAKRLSGFEMNAVYCDSV 177 Query: 175 IIDG-DFPAYVER-VGREDLFRQAHVVSLHTPLNDETRNMIDASLLALLPPQSYLVNSAR 232 ++ D A+ R V ++L + V P+ +T ++IDA ++ + SYL+N++R Sbjct: 178 PLNPVDEQAWNARQVSFDELLTCSDFVVPMLPMTSDTFHLIDAHAISKMRRGSYLLNTSR 237 Query: 233 GGLIQIDDLLAALENGQLKGAALDVLPVE-------PPTTSAAIVQH-PRVLLSPHAAFF 284 G ++ + ++ AL G L G A DV +E P T A++ + + L +PH Sbjct: 238 GSVVDENAVVEALNQGHLAGYAADVFEMEEWARPDRPLTVPQALLNNRTQTLFTPHVGSG 297 Query: 285 SDVAARELRRKAAQNLIDWDRNGRP 309 E+ R +A +++ RP Sbjct: 298 VKKVRLEIERYSAHSILQALAGQRP 322 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 730,643 Number of extensions: 28653 Number of successful extensions: 98 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 95 Number of HSP's successfully gapped: 4 Length of query: 319 Length of database: 1,221,021 Length adjustment: 83 Effective length of query: 236 Effective length of database: 914,170 Effective search space: 215744120 Effective search space used: 215744120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)