BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3259.p (466 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980460|ref|YP_001353862.1| 3-isopropylmalate dehydratase l... 881 0.0 gi|152980040|ref|YP_001354033.1| isopropylmalate isomerase large... 465 e-132 gi|152980286|ref|YP_001353191.1| aconitate hydratase [Janthinoba... 89 4e-19 >gi|152980460|ref|YP_001353862.1| 3-isopropylmalate dehydratase large subunit [Janthinobacterium sp. Marseille] Length = 468 Score = 881 bits (2277), Expect = 0.0, Method: Compositional matrix adjust. Identities = 424/466 (90%), Positives = 438/466 (93%) Query: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 M +TLYDKLW+SHVV T DDGT +LYIDRHLLHEVTSPQAFEGLKLAGR+PWR SANLMV Sbjct: 1 MAQTLYDKLWQSHVVETGDDGTTVLYIDRHLLHEVTSPQAFEGLKLAGRRPWRVSANLMV 60 Query: 61 ADHNVPTTDRAQGIADPISRLQVETLDGNAKEFSLTYFGMNDKRQGIVHVIGPEQGATLP 120 ADHNVPTTDRA GIADP SRLQVETLDGNA E+ LTYF M DKRQGIVHVIGPEQGATLP Sbjct: 61 ADHNVPTTDRANGIADPTSRLQVETLDGNAHEYGLTYFSMRDKRQGIVHVIGPEQGATLP 120 Query: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 GMTVVCGDSHTSTHGAF LAHGIGTSEVEHVLATQTLLA+KSKAMLVQVDGALP GVTA Sbjct: 121 GMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQTLLAQKSKAMLVQVDGALPDGVTA 180 Query: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMT+CNMAIEAGARAGMVA DD T+ Sbjct: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTICNMAIEAGARAGMVAVDDITI 240 Query: 241 EYLKGRPFSPSGPHWERAVSYWRTLHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMVVAV 300 Y+KGRP SP GPHW+RAV YWRTLHSD GAKFDLVVTLNAAEIKPQV+WGTSPEMVVAV Sbjct: 241 NYVKGRPLSPVGPHWDRAVEYWRTLHSDAGAKFDLVVTLNAAEIKPQVSWGTSPEMVVAV 300 Query: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 D RVPDPDKEKDPTKRDGMEKAL YMALKPNTPI DIRIDKVFIGSCTNSRIEDLR AA Sbjct: 301 DGRVPDPDKEKDPTKRDGMEKALIYMALKPNTPITDIRIDKVFIGSCTNSRIEDLRAAAA 360 Query: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 VVRGK+RASNVKLAMVVPGSGLVKEQAEREGLDKIF+ AGFEWREPGCSMCLAMNADRLE Sbjct: 361 VVRGKYRASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADRLE 420 Query: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGI GHFVDVR+L Sbjct: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIEGHFVDVRAL 466 >gi|152980040|ref|YP_001354033.1| isopropylmalate isomerase large subunit [Janthinobacterium sp. Marseille] Length = 468 Score = 465 bits (1197), Expect = e-132, Method: Compositional matrix adjust. Identities = 244/467 (52%), Positives = 305/467 (65%), Gaps = 12/467 (2%) Query: 3 KTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMVAD 62 +TLY KL +SH V D+ +LY+D H+++E TSPQAF GL+ GR R L V Sbjct: 4 RTLYQKLVDSHTVSRLDEQHVLLYVDLHIMNEYTSPQAFAGLRAQGRTVLRPRQQLAVVS 63 Query: 63 HNVPTTDRAQG---IADPISRLQVETLDGNAKEFSLTYFGMNDKRQGIVHVIGPEQGATL 119 H +PT G I DP S LQ L N E+ + ND QGI H+I PE G Sbjct: 64 HIIPTHPVQVGQRVIFDPPSALQATNLKKNCTEYGIKLLDTNDAEQGIEHIIAPEHGWVR 123 Query: 120 PGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVT 179 PGM V+CGDSHT+T+GA AL GIGTSEVEH+LATQTL+ R + M ++VDG LP G + Sbjct: 124 PGMVVLCGDSHTTTYGALGALGFGIGTSEVEHILATQTLVYRLADNMRIRVDGELPVGTS 183 Query: 180 AKDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTT 239 KD+VL +I +IG G GYA+EF GSTI +LS E RMT+CNMA+EAGARA ++ D TT Sbjct: 184 VKDLVLLIISRIGAQGARGYAVEFCGSTIAALSAEARMTLCNMAVEAGARAALIRPDATT 243 Query: 240 LEYLKGRPFSPSGPHWERAVSYWRTLHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMVVA 299 Y+K + +A W+TL+SD+ A F++ +A E+ P VTWGTSP+ + Sbjct: 244 FAYIKAHATDLDEAAYAQAQQDWQTLYSDEDAAFEVDHQFDAREVAPYVTWGTSPDQAIP 303 Query: 300 VDARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAA 359 V+ +PD + + KAL YM L + + I++VFIGSCTN RIEDLR AA Sbjct: 304 VNGHIPDTAQNDN--------KALHYMGLSAGASLLGLPIERVFIGSCTNGRIEDLRIAA 355 Query: 360 KVVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRL 419 ++ G+ A+ V+ AMVVPGSG V+ QAE+EGL IF AGFEWR+PGCSMCLAMN D L Sbjct: 356 GIIAGQHVANGVR-AMVVPGSGAVRRQAEQEGLADIFIAAGFEWRQPGCSMCLAMNDDVL 414 Query: 420 EPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 PGERCAST+NRNFEGRQG GRTHL+SPAMAAAA I G DVR L Sbjct: 415 TPGERCASTTNRNFEGRQGREGRTHLMSPAMAAAAAIRGCISDVRDL 461 >gi|152980286|ref|YP_001353191.1| aconitate hydratase [Janthinobacterium sp. Marseille] Length = 907 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 97/364 (26%), Positives = 151/364 (41%), Gaps = 30/364 (8%) Query: 123 TVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTAKD 182 T+V DSHT+ + G+G E E + Q + + V + G L GVTA D Sbjct: 204 TLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGQLREGVTATD 263 Query: 183 IVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEY 242 +VL + + G +EF G SLS+ R T+ NMA E GA G DD T++Y Sbjct: 264 LVLTITEMLRKEKVVGKFVEFFGEGTESLSLTDRATIANMAPEYGATMGFFPVDDATIDY 323 Query: 243 LKGRPFSPSGPHWERAVSYWRT-----LHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMV 297 KG + + SY++ + + VV L+ + P + P+ Sbjct: 324 FKGT--GRTKAEIDAFESYFKAQGLYGVPKSGEIDYTHVVALDLGSVAPSLAGPKRPQDR 381 Query: 298 VAV-DARVPDPDKEKDPTKRDGMEKALAYM-ALKPNTPIEDIRIDKVFIG---SCTNSRI 352 + + + + + PT +G K + + A N+ ++ V I SCTN+ Sbjct: 382 IEIGNVKHNFAELFAKPTAENGFNKKIEDLDATYTNSDGVKVKNGDVLIAAITSCTNTSN 441 Query: 353 EDLREAAKVVRGKFRASNVKLA-----MVVPGSGLVKEQAEREGLDKIFRDAGFEWREPG 407 + A ++ K + +K++ + PGS +V E E GL GF G Sbjct: 442 PSVLLTAGLLAKKAVEAGLKVSPHIKTSLAPGSRVVTEYLEAAGLLPYLEKLGFGVTAYG 501 Query: 408 CSMCLAMNADRLEPGERCAS-----------TSNRNFEGRQGAGGRTH-LVSPAMAAAAG 455 C+ C+ NA L P A + NRNFE R R++ L SP + A Sbjct: 502 CTTCIG-NAGDLTPAMNEAIVQHDIVASAVLSGNRNFEARIHPNIRSNFLASPPLVVAYA 560 Query: 456 IAGH 459 IAG+ Sbjct: 561 IAGN 564 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,061,905 Number of extensions: 43375 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 3 Length of query: 466 Length of database: 1,221,021 Length adjustment: 87 Effective length of query: 379 Effective length of database: 899,382 Effective search space: 340865778 Effective search space used: 340865778 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)