BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3257.p (356 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979812|ref|YP_001353859.1| 3-isopropylmalate dehydrogenase... 619 e-179 gi|152979772|ref|YP_001352927.1| tartrate dehydrogenase [Janthin... 167 1e-42 gi|152980642|ref|YP_001355189.1| isocitrate dehydrogenase (NAD+)... 154 1e-38 >gi|152979812|ref|YP_001353859.1| 3-isopropylmalate dehydrogenase [Janthinobacterium sp. Marseille] Length = 356 Score = 619 bits (1597), Expect = e-179, Method: Compositional matrix adjust. Identities = 299/356 (83%), Positives = 323/356 (90%) Query: 1 MKIAILPGDGIGPEIVDEAVRVLNALDEKFEMETAPVGGAGYEAHGHPLPDGTLKLAKEA 60 MKIAILPGDGIGPEI+++AV+V+NAL EKFE ET PVGGA Y A GHPLP+ TLK+AKEA Sbjct: 1 MKIAILPGDGIGPEIMNQAVKVMNALGEKFETETVPVGGAAYAAQGHPLPESTLKVAKEA 60 Query: 61 DAILFGAVGDWKYDKLERALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA 120 DAILFGAVGD+KYD LER+LRPEQAILGLRKHL LFANFRPAI YPEL GASSLKPE+V+ Sbjct: 61 DAILFGAVGDFKYDTLERSLRPEQAILGLRKHLGLFANFRPAILYPELAGASSLKPEIVS 120 Query: 121 NLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK 180 LDI+IVREL GDIYFGQPRG+R APDGPFKG REGFDTMRY+EPEIRRIAHVAFQAA K Sbjct: 121 GLDIMIVRELTGDIYFGQPRGVRAAPDGPFKGEREGFDTMRYTEPEIRRIAHVAFQAAQK 180 Query: 181 RGKRLCSVDKANVLETFQFWKDIVTEVGKEYPDVELSHMYVDNAAMQLVKAPKNFDVIVT 240 RGKRL SVDKANVLETFQFW+DIVT+V KEY DV+L HMYVDNAAMQLV+APK FDV+VT Sbjct: 181 RGKRLTSVDKANVLETFQFWRDIVTDVHKEYQDVKLDHMYVDNAAMQLVRAPKEFDVVVT 240 Query: 241 GNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATILSA 300 GNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGK IANPLA ILS Sbjct: 241 GNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKDIANPLAQILSL 300 Query: 301 AMMLRFSLNRAEQADRIENAVKKVLAQGLRTADIYEEGCTKVNTQQMGEAVVKALA 356 AMMLR+SL + EQADRIE AVK VL QGLRT DI+E G TKV+T QMG+AVVKALA Sbjct: 301 AMMLRYSLGKTEQADRIETAVKSVLKQGLRTVDIFEAGTTKVSTTQMGDAVVKALA 356 >gi|152979772|ref|YP_001352927.1| tartrate dehydrogenase [Janthinobacterium sp. Marseille] Length = 358 Score = 167 bits (422), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 129/376 (34%), Positives = 182/376 (48%), Gaps = 44/376 (11%) Query: 2 KIAILPGDGIGPEIVDEAVRVLNALDEKFEM--ETAPVGGAG---YEAHGHPLPDGTLKL 56 KIA++ GDGIG E++ E +RVL A KF + E + A Y HG +PD + Sbjct: 5 KIAVIAGDGIGNEVMPEGLRVLEAAARKFNLALEFTTMEWANCEYYLKHGQMMPDDWRQQ 64 Query: 57 AKEADAILFGAVGDWKYDKLERALRPEQ-----AILGLRKHLQLFANFRPAICYPEL-TG 110 + +AI FGA G AL P+ +++ R+ + N RP P + Sbjct: 65 LEGFEAIYFGAAG-------MPALVPDHISLWGSLIKFRRDFDQYVNLRPVRLMPGVPCP 117 Query: 111 ASSLKPELVANLDILIVRELNGDIYFGQPRGMREAPDG-PFKGAREGFDTMR--YSEPEI 167 ++ KP ++D +VRE G A G F+G F + +S Sbjct: 118 LANRKP---GDIDFYVVRE--------NTEGEYSAVGGKSFEGTEYEFVSQNAIFSRHGT 166 Query: 168 RRIAHVAFQAAAKRGKR-LCSVDKANVLE-TFQFWKDIVTEVGKEYPDVELSHMYVDNAA 225 RI AF+ A R K+ L S K+N + + +W V + Y DV ++D Sbjct: 167 DRILKYAFELAQSRPKKHLTSATKSNGISISMPYWDSRVEAMAPSYADVHWDKYHIDILC 226 Query: 226 MQLVKAPKNFDVIVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNK--GLYEPSHGSAP 283 + V +P+ FDV+V N+FGDILSD TG+IG+ PSA+L+ K L+EP HGSAP Sbjct: 227 ARFVLSPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERKFPSLFEPVHGSAP 286 Query: 284 DIAGKGIANPLATILSAAMMLRF----SLNRAEQADRIENAVKKVLAQGLRTADIYEEGC 339 DI GK IANP+ I S AMML F N D I A++ VL QG T D+ G Sbjct: 287 DIFGKNIANPIGMIWSGAMMLDFLGNGDANYKAAHDAIMQAIETVLVQGPNTPDLGGSG- 345 Query: 340 TKVNTQQMGEAVVKAL 355 NT +G+A+ A+ Sbjct: 346 ---NTTDVGKAIAAAI 358 >gi|152980642|ref|YP_001355189.1| isocitrate dehydrogenase (NAD+) [Janthinobacterium sp. Marseille] Length = 349 Score = 154 bits (388), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 126/338 (37%), Positives = 170/338 (50%), Gaps = 24/338 (7%) Query: 1 MKIAILPGDGIGPEIVDEAVRVLNALDEKFEMETAPVGGAGYEAHGHPLPDGTLKLAKEA 60 + + ++PGDGIGPEIVD VRV +AL F ET G E G LP TL Sbjct: 10 IPVTLIPGDGIGPEIVDVVVRVFDALGNPFAWETQQAGVNALEKSGDLLPQTTLDSIGRT 69 Query: 61 DAILFGAVGDWKYDKLERALRPEQAILGLRKHLQLFANFRPAICYPELTGASSLKPELVA 120 L G + + R + LR+ QL+AN RPA + G K Sbjct: 70 GLALKGPLST----PIGGGFRSVN--VRLRETFQLYANVRPARTI--VPGGRYEK----- 116 Query: 121 NLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIAHVAFQAAAK 180 +D+++VRE +Y G + D G +T S RRI+ AF A + Sbjct: 117 -IDLVLVRENLEGLYVGHEHYVPIGDDAHAVAMATGINTRAGS----RRISKFAFDYAVR 171 Query: 181 RGKRLCS-VDKANVLETFQ-FWKDIVTEVGKEYPD-VELSHMYVDNAAMQLVKAPKNFDV 237 +R + V KANVL+ + + +VG Y D +E + VD AMQLV P FDV Sbjct: 172 NNRRKVTIVHKANVLKALTGLFLETAKQVGLNYADQIEFNDRIVDACAMQLVLNPWQFDV 231 Query: 238 IVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATI 297 IV+ N+FGDILSD A L G +GM P A++ ++E HGSAPDIAGKG+ANP A + Sbjct: 232 IVSTNLFGDILSDQIAGLVGGLGMAPGANI-GERAAIFEAVHGSAPDIAGKGMANPTALL 290 Query: 298 LSAAMMLRFSLNRAEQADRIENAVKKVLA-QGLRTADI 334 L+AA+ML QA + +A+ VL + RTAD+ Sbjct: 291 LAAALMLEHK-GLHGQAKFLRDAIDAVLGEESGRTADL 327 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 866,021 Number of extensions: 38156 Number of successful extensions: 119 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 3 Length of query: 356 Length of database: 1,221,021 Length adjustment: 84 Effective length of query: 272 Effective length of database: 910,473 Effective search space: 247648656 Effective search space used: 247648656 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)