BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3227.p (523 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982589|ref|YP_001352814.1| major facilitator superfamily m... 421 e-119 gi|152980367|ref|YP_001352036.1| major facilitator superfamily d... 146 3e-36 gi|152982526|ref|YP_001355122.1| major facilitator superfamily d... 130 1e-31 gi|152982183|ref|YP_001354785.1| major facilitator transporter [... 112 4e-26 gi|152982562|ref|YP_001352191.1| major facilitator superfamily d... 82 1e-16 >gi|152982589|ref|YP_001352814.1| major facilitator superfamily multidrug resistance B translocase [Janthinobacterium sp. Marseille] Length = 517 Score = 421 bits (1081), Expect = e-119, Method: Compositional matrix adjust. Identities = 230/500 (46%), Positives = 327/500 (65%), Gaps = 4/500 (0%) Query: 17 PGPLEAPLSGGQLVGAGILLAAANFIAVLDTTIANVSVSTISGALGASTSQGTYVITSYA 76 P + PLSG L+ + L+ A F+ VLD++IANVS+ ISG LG S QGT+VITS++ Sbjct: 5 PRSVVPPLSGTPLIVGTLALSLAVFMNVLDSSIANVSIPAISGDLGVSPQQGTWVITSFS 64 Query: 77 VAEAITVPLTGWLANRFGTVRVFILSMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPL 136 VA AI+VPLTGWL R G VR+FI S+++F L S LCG A S+ +LIA RV+QG GP+ Sbjct: 65 VANAISVPLTGWLTQRIGQVRLFIGSILLFVLASCLCGFAPSIEILIAARVLQGAVAGPM 124 Query: 137 MPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVAL 196 +PLSQ LLL +P K+ A+ W MTTLVAPI GP+LGG + D Y+WP+IF+IN+P+ L Sbjct: 125 IPLSQALLLSSYPPAKSGMALAFWGMTTLVAPIMGPLLGGWISDNYTWPWIFFINIPIGL 184 Query: 197 ICGYLGWRMLKRFETKTSKLPIDRIGLILMVVWVAALQLMLDEGKEKDWFASTEIVLLAI 256 + W + K E++T KLPID++GL+L+V+WV +LQLMLD+GKE DWF S EI++LA Sbjct: 185 FAAWATWSIYKERESRTYKLPIDKVGLVLLVLWVGSLQLMLDKGKELDWFHSGEIIILAS 244 Query: 257 TAVVFFIAFLIWELTEHNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYM 316 AVV FI F+IWE+ E +P+VDL +F+ R F+ V I +A+G FFGS+V+ PLWLQ + Sbjct: 245 VAVVAFIYFVIWEIGEEHPVVDLSLFKGRNFSGGVIAISVAYGLFFGSLVILPLWLQTQI 304 Query: 317 GYTATWSGCISALTGILAVMTAPLVAKMSATIDARKLVCFGVTWLGLITFVRAFNTTDMS 376 GYTAT +G + A GI A++ +P+V KM IDAR + L+ F+R+ T D+ Sbjct: 305 GYTATEAGKVMAPVGIFAIILSPIVGKMLPKIDARWVATTAFLIFSLVFFMRSQFTQDVD 364 Query: 377 FFQIGWPLFLQGIGLPLFFVPLTSLALSSVNEEETASAAGLMSFCRTMSGAIATSIVNTS 436 + P +QG + +FF+PLTS+ LS ++ +AAGL +F R M G I TS+++T Sbjct: 365 MMTLMIPTVIQGAAMAMFFIPLTSIILSGQAPDKIPAAAGLSNFVRIMFGGIGTSVMSTM 424 Query: 437 WENDATHFHAELAGLVDRS----GAAIQSMMASGMSLEQATASLAQTVQNQAVMLSTNHI 492 W+N HA+LA G A+ ++M+ GMS + A A + + + QA L+ + Sbjct: 425 WDNRTILHHAQLAEYTGAHNPAFGQAVSALMSRGMSEQAAWAVIDRQMTVQASTLAATDL 484 Query: 493 FMMAAVSFSLAALAVWLAPK 512 F M+A+ F +W+ + Sbjct: 485 FWMSAIMFLCLIGFIWITKR 504 >gi|152980367|ref|YP_001352036.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 478 Score = 146 bits (368), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 81/257 (31%), Positives = 153/257 (59%), Gaps = 2/257 (0%) Query: 35 LLAAANFIAVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 94 ++A A F+ LD+TI N ++ +++ +L + V++SY +A A+++P++GW+A+RFG Sbjct: 23 VVAIALFMEQLDSTIINTAIPSMAASLHVTPLSLKAVVSSYILALAVSIPISGWMADRFG 82 Query: 95 TVRVFILSMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAP 154 T RVF +++ +F S LCGL+ ++ ML+A R++QG + +MP+ + ++R FPK + Sbjct: 83 TRRVFSVAVGLFTFSSILCGLSVNVPMLVAARILQGFSAAMMMPVGRLTVIRTFPKSELL 142 Query: 155 AAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTS 214 AA+ + L+ P+ GP +GG++ W IF++NVP+ L ++ R + + + + Sbjct: 143 AAMNFVIIPALIGPLLGPTVGGLIVHWLPWQAIFFVNVPIGLAAQWMIHRHMPDYRSDSP 202 Query: 215 KLPIDRIGLILMVVWVAALQLMLDEGKEKDWFASTEIVLLAITAVVFFIAFLIWELTEHN 274 + P+D IGL+L VA L +L+ E + +T +L I ++ A+++ + Sbjct: 203 R-PLDVIGLVLFGSGVALLSWILEIFGEH-YLDATSTTILFIISLALLGAYILHARRTLH 260 Query: 275 PIVDLRVFRHRGFTASV 291 P++ L +F+ R F SV Sbjct: 261 PLLQLTLFKTRTFRVSV 277 >gi|152982526|ref|YP_001355122.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 481 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 127/485 (26%), Positives = 222/485 (45%), Gaps = 40/485 (8%) Query: 43 AVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILS 102 A+L +TI NV++ +S + +V T + +A +++ LT WL RFG R + + Sbjct: 32 AILSSTIVNVAIPDLSRYFVLGQERAQWVSTGFMLAMTLSMLLTPWLLLRFGLRRTYGGA 91 Query: 103 MIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAM 162 + + + + G + + ML++ RV++GLA G + P+ ++LR F + + A+G++ Sbjct: 92 ICLLLIGGVVGGFSINYPMLLSMRVLEGLASGVMQPIPAIVILRAFDQHEQGKAMGIFGF 151 Query: 163 TTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRF-ETKTSKLPIDRI 221 ++AP GP +GG L + + W IF++ VP L+ L R L + S P+D Sbjct: 152 GVVLAPAVGPSVGGFLVELFGWRSIFFVVVPPCLVVLVLIQRFLASYTPVPDSDKPLDWK 211 Query: 222 GLILMVVWVAALQLMLDEGKEKDWFASTEIVLLAITAVVFFIAFLIWE-LTEHNPIVDLR 280 GL+L+ V + +L L+ E +VLL I V I F++++ T P+++++ Sbjct: 212 GLLLIGVSIVSL---LNGVVELRGDMPQAVVLLVI-GVATLIGFILYQTRTSAEPLMNMQ 267 Query: 281 VFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPL 340 +FRHR F + FGS L P+++Q M Y + +G + GI+ +T PL Sbjct: 268 LFRHRQFAMGALVAFIYGMGLFGSTYLLPIYMQMGMNYAPSQAGLVLLPAGIVLAVTIPL 327 Query: 341 VAKMSATIDARKLVCFGVTWLGLITFVRAFNTTDMSFFQI-GWPLFLQGIGLPLFFVPLT 399 +++ LV G+ L + A + S+ + W + + IGL F +P Sbjct: 328 AGRLADRYPPNVLVAIGLFLLAASFALMANGSPATSYLALMAWAV-IGRIGLG-FVLPAL 385 Query: 400 SL-ALSSVNEEETASAAGLMSFCRTMSGAIATSIVNTSWENDATHFHAELAGLVDRSGAA 458 SL A+ ++ A A ++F R + GAI S+V E Sbjct: 386 SLGAMRGLDIGLVAQGASAINFVRQLGGAIGVSLVGVVLEWR------------------ 427 Query: 459 IQSMMASGMSLEQATASLAQTVQNQAVMLSTNHIFMMAAVSFSLAALAVWLAPKPTRVAD 518 ++L QA ++L A N F + AV ++A +A W + D Sbjct: 428 --------IALHQAQSALGTDSHRNAF----NETFFLVAVLCAIAVVAAWYMREKPMTKD 475 Query: 519 TTAAH 523 AA Sbjct: 476 QEAAE 480 >gi|152982183|ref|YP_001354785.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 469 Score = 112 bits (281), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 104/394 (26%), Positives = 178/394 (45%), Gaps = 8/394 (2%) Query: 42 IAVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFIL 101 +A LDT IAN ++ ++ L A+ + +++ Y +A T+ L G R+ I Sbjct: 42 MASLDTAIANTALPAMAMQLNATPADSVWIVNIYQLAMVATLLPFAALGEILGYRRIAIF 101 Query: 102 SMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWA 161 +++F S C A SL L+ R QG+ +M ++ LL I+P +G A Sbjct: 102 GLLLFTAASLACAYAWSLPTLVIARFFQGVGASAIMGVNTALLRSIYPARMLGRGLGTNA 161 Query: 162 MTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTSKLPIDRI 221 + VA GP ++ +SWP++F INVP+ L +L ++L + T K D + Sbjct: 162 LVVAVAFAVGPTTASLILSVWSWPWLFAINVPLGLFAYFLARKVLPHTKRATHKF--DAL 219 Query: 222 GLILMVVWVAALQLMLDEGKEKDWFASTEIVLLAITAVVFFIAFLIWELTEHNPIVDLRV 281 I L L + EG + + L A+VFFI L + P++ + + Sbjct: 220 TAIYNAGGFGLLILAIGEGAHLS--DAKTVALELFGALVFFILLLRRQKGHAAPMLPVDL 277 Query: 282 FRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPLV 341 FR FT S T F A + V P + + +G +A +G + ++ + AP+ Sbjct: 278 FRRPLFTLSALTAVCTFAAQSLAFVSLPFYFETVLGRSAIETGFLITPWAVMVGIMAPIA 337 Query: 342 AKMSATIDARKLVCFGVTWL--GLITFVRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLT 399 ++S L G++ L G+++ + + D+S F I W + L GIG F P Sbjct: 338 GRLSDHYPPGMLGGVGLSVLSAGMVSLI--LMSPDVSVFGICWRMALCGIGFGFFQAPNL 395 Query: 400 SLALSSVNEEETASAAGLMSFCRTMSGAIATSIV 433 +SS E SA+G+++ R + A ++V Sbjct: 396 RAIMSSAPPERGGSASGVVATSRLLGQASGAALV 429 >gi|152982562|ref|YP_001352191.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 518 Score = 81.6 bits (200), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 64/257 (24%), Positives = 126/257 (49%), Gaps = 9/257 (3%) Query: 35 LLAAANFIAVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 94 +++ A + V+D T+ ++ ++ L AS S +++ YA+ + + G L +R G Sbjct: 26 IVSVALLLIVVDMTVLYAALPRLTADLTASASAKLWIVNIYALTVSGLLLGMGTLGDRLG 85 Query: 95 TVRVFILSMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAP 154 +FI +++FG+ S + S LIA R + + +MP + +++ F E Sbjct: 86 HKSLFIAGLVVFGIASLCAAYSPSSATLIASRALLAVGAAMMMPATLSIIRLAFADENER 145 Query: 155 A-AVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKT 213 A A+G+WA GP++GG+L + + W +F INVP+ L+ + + + + Sbjct: 146 AVAIGIWASVAFGGAALGPVVGGLLLEYFWWGSVFLINVPIVLVALPIALAYIPKGKANK 205 Query: 214 SKLPIDRIGLILMVVWVAALQLMLDEG--KEKDWFASTEIVLLAITAVVFFIA-FLIWEL 270 ++ P D IG + ++ + + L E +E W I LL + +FF+A F + Sbjct: 206 TR-PWDLIGSLQIMAGLVGVTYALKEAGKREPAW----GIALLVLLVGIFFLAVFARRQQ 260 Query: 271 TEHNPIVDLRVFRHRGF 287 +P++D +F++ F Sbjct: 261 HSAHPMIDFSLFKNTIF 277 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.325 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,181,153 Number of extensions: 48537 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 302 Number of HSP's successfully gapped: 13 Length of query: 523 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 435 Effective length of database: 895,685 Effective search space: 389622975 Effective search space used: 389622975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)