BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3145.p (488 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981317|ref|YP_001351712.1| outer membrane protein, tolC-li... 163 2e-41 gi|152981001|ref|YP_001354628.1| drug efflux lipoprotein [Janthi... 160 2e-40 gi|152980456|ref|YP_001353256.1| outer-membrane drug efflux prot... 147 1e-36 gi|152980931|ref|YP_001355168.1| outer membrane efflux protein [... 142 7e-35 gi|152981442|ref|YP_001353371.1| outer membrane protein, tolC-li... 135 6e-33 >gi|152981317|ref|YP_001351712.1| outer membrane protein, tolC-like [Janthinobacterium sp. Marseille] Length = 475 Score = 163 bits (413), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 163/482 (33%), Positives = 241/482 (50%), Gaps = 34/482 (7%) Query: 15 ALAAAALLAGCALGPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPVNRWWQGLNDAELDS 74 +L A +L GCA+GP+Y P QA +A + + WW+ DAEL++ Sbjct: 11 SLLALLILGGCAVGPDYQRPDAPVAQAWKTEAGWQASNPRDGELK-GAWWEIFRDAELNA 69 Query: 75 LIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPPANLGALTGGS 134 L E+AL+ + IA+AR+ Q+RA + ++ P +GA AG AR + LT + Sbjct: 70 LQEQALKNGQTMVIAQARVDQARAQANVANSSFFPKIGAQ--AGSARNRTSAERPLTNYN 127 Query: 135 SSGNGSATSANLYSAGFDATWEADLFGAHRRAIQAAGATAQAAEASLADVQVSLAAEVAN 194 S S+ N ++A F ++E DLFG RR + AGATAQ A+A + ++ L AE+A Sbjct: 128 S--QNSSVVQNDFNAAFTVSYELDLFGRVRRQAEGAGATAQQAQADFENTRLVLTAELAA 185 Query: 195 AYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQLDASRADLIPL 254 Y+ LR + + +Q + AQ + L S R G AS L+ + Q A++ L L Sbjct: 186 DYLVLRQTDAEIDVLQQTLGAQTKALDFISARHELGAASGLDLNQQQGLTAATQTQLTLL 245 Query: 255 QADREAYLNQLATLLGQEPGTLD---NELTATAP-VPLPPQQVAVGDPVTLLRRRPDVRA 310 Q R Y N +ATL+G N TAP +PL P LL RRPD+ A Sbjct: 246 QDQRARYQNAIATLVGVPAPDFKLPVNLAVTTAPAIPLTA-------PAALLERRPDIAA 298 Query: 311 AERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGAPMLSWN---- 366 AER +AA A+IG A +A +PSL GL G + SD +L + AP L W+ Sbjct: 299 AERAMAAANAQIGVARSAYFPSL------GLSGLYGSDANQLSNL--FSAPALLWSIGVS 350 Query: 367 ----FLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSLSRFRYGRVTVATLART 422 D GR A V A+ +A YRQ+VL A+++ +D L+ T A+ Sbjct: 351 ATQMIFDGGRTSANVDFAKAGYQQALGNYRQSVLVAMQEVQDGLNS-DVALAQALTSAKV 409 Query: 423 KA-SADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGL 481 A SADR+L L+ RYQ+G T +E++ + ++ ++ +Q + V + KALG Sbjct: 410 AADSADRSLGLSTDRYQSGITTNLEVVVAQQTFLSYKRQEVQLHGQRLVNTVKLIKALGG 469 Query: 482 GW 483 GW Sbjct: 470 GW 471 >gi|152981001|ref|YP_001354628.1| drug efflux lipoprotein [Janthinobacterium sp. Marseille] Length = 481 Score = 160 bits (404), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 144/481 (29%), Positives = 229/481 (47%), Gaps = 37/481 (7%) Query: 14 IALAAAALLAGCALGPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPVNRWWQGLNDAELD 73 +AL AA +L+ C++ P+Y P AP AA G +A ++ A+ + W D L Sbjct: 6 LALLAAGVLSACSMAPKYVRPD--APVAADYGVPSKADNNQVRAVDIG-WRNFFTDPRLQ 62 Query: 74 SLIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPPANLGALTGG 133 LI A+ N +L +A R++++RA ++ A+ P++ +A AR+P Sbjct: 63 VLIGNAIANNRDLRVAALRIEEARALYNIQSADLLPTLNVNATGSRARVP---------A 113 Query: 134 SSSGNGSATSANLYSAGFD-ATWEADLFGAHRRAIQAAGATAQAAEASLADVQVSLAAEV 192 S S GS+ ++ Y G A +E D FG R AA A + E + Q+SL +EV Sbjct: 114 SISNTGSSVVSSNYQVGLGLAAFELDFFGRVRSLSDAALAAYLSTEEAQRAAQISLVSEV 173 Query: 193 ANAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQLDASRADLI 252 A AY+S R ++ L Q +++ + KL+ QR G +S+L+ + L +RA + Sbjct: 174 AKAYLSERSFAEQQDLARQTYESRVEAYKLSKQRFEVGASSALDLRNDEALLQTARAAEL 233 Query: 253 PLQADREAYLNQLATLLGQEPGTLDNELTATAPVPLPPQQ----------VAVGDPVTLL 302 Q R +N L L+GQ P T LPP Q + G P LL Sbjct: 234 LAQRQRAQAMNALTLLVGQ-PLT-----------DLPPAQALSSQTILTDIPAGLPSDLL 281 Query: 303 RRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGAPM 362 +RPD+R+AE+TL + A IG A AA +P + L +G + L + AP Sbjct: 282 TQRPDIRSAEQTLLSANANIGAARAAFFPRISLTAGLGTSSNELAGLFEGGSRSWSFAPQ 341 Query: 363 LSWNFLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSL-SRFRYGRVTVATLAR 421 L D GR + + AE +D A Y +++ A R+ D+L +R + A Sbjct: 342 LVLPIFDAGRNRGNLNLAEVRKDIAVANYEKSIQVAFREVSDALVARDLLDQQVEAQRLV 401 Query: 422 TKASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGL 481 +A A+R + A QR+Q G + +++LD R+ TAQ +L+Q + V + ++LG Sbjct: 402 QEAQAER-FKFATQRFQNGIASSLDVLDAQRELFTAQLSLVQVRLTRLTNAVDLYRSLGG 460 Query: 482 G 482 G Sbjct: 461 G 461 >gi|152980456|ref|YP_001353256.1| outer-membrane drug efflux protein [Janthinobacterium sp. Marseille] Length = 485 Score = 147 bits (372), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 147/490 (30%), Positives = 227/490 (46%), Gaps = 46/490 (9%) Query: 13 LIALAAAALLAGCALGPEYAGPPEAA----PQAAHRGHFVRAGD---SATTAMPVNR--- 62 L + AA +LAGC+ A PPE P A V+A + + A P + Sbjct: 8 LSTMVAALVLAGCST----ARPPEELKLDLPNAYREQSTVKANELDGAWKLAAPADNKDR 63 Query: 63 --WWQGLNDAELDSLIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHA 120 WW+ D +L +LI A +N NL IA AR++ +RA + A+ V A Sbjct: 64 GAWWKVFGDEQLSNLIVEAGNSNLNLAIALARVKDARAIAGVVDADRGVQVDAG------ 117 Query: 121 RLPPANLGALTGGSSSGNGSATSANLYSAGFDATWEADLFGAHRRAIQAAGATAQAAEAS 180 G + SGN S Y A +E DLFG A +AA A+ EA+ Sbjct: 118 ----------VGPTRSGNAQGASTQ-YRAPLSFAYEVDLFGRLSDASRAARLDAEGQEAA 166 Query: 181 LADVQVSLAAEVANAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRI 240 V ++L ++VA Y ++R + + E+ V +++ L LT +R G S L+ + Sbjct: 167 YRSVLLALQSDVAQQYFTIRSLDSEVDVLEKTVALRQEALDLTQRRFDAGDTSELDVAQA 226 Query: 241 QNQLDASRADLIPLQADREAYLNQLATLLGQEPGTLDNELTATAPV-PLPPQQVAV--GD 297 + + + A+L ++ R + LA LLG+ P T PV PL V V G Sbjct: 227 KTEWATTSAELEGVKRQRAQRDHALAILLGKAPAEF------TLPVAPLAAAVVTVPSGL 280 Query: 298 PVTLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAA 357 P LL RRPD+ A+R LAA +A+IG A+AA +P L L G G DL + + Sbjct: 281 PSDLLERRPDIAQAQRQLAASSARIGAAKAAFFPKLLLTGSAGYESNELRDLFKWSSRSW 340 Query: 358 IGAP----MLSWNFLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSLSRFRYGR 413 + P +LS D GR ++ + +A+ + YR VL +D ED+LS R Sbjct: 341 MLGPVVGTILSMPLFDGGRNQSNLARADANYEALVANYRLQVLVGFQDVEDNLSALRTLD 400 Query: 414 VTVATLARTKASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFV 473 + + SA A LA RY+ G+ + E++D R +++Q++ +++ + V Sbjct: 401 RQLQFEEQAVNSAQLASRLADTRYRYGSASYFEVIDAQRSSLSSQRSKIRSTGQRAVASV 460 Query: 474 GIQKALGLGW 483 G+ +ALG GW Sbjct: 461 GLIRALGGGW 470 >gi|152980931|ref|YP_001355168.1| outer membrane efflux protein [Janthinobacterium sp. Marseille] Length = 473 Score = 142 bits (357), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 148/477 (31%), Positives = 219/477 (45%), Gaps = 28/477 (5%) Query: 13 LIALAAAALLAGCAL-GPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPVNRWWQGLNDAE 71 L +AA LLAGCA + PE APQ V A + TT++ WW+ +E Sbjct: 4 LTGFSAALLLAGCATQATQQTLVPEVAPQWRQPQANVDA--TGTTSVLTRDWWRSFGSSE 61 Query: 72 LDSLIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPP--ANLGA 129 LDSL+E + ++ A AR+ Q++A + +AG A LP N GA Sbjct: 62 LDSLLETVQVQSYDIAAAVARVTQAQA--------------TATIAGAALLPELNGNFGA 107 Query: 130 LTGGSSSGNGSAT-SANLYSAGFDATWEADLFGAHRRAIQAAGATAQAAEASLADVQVSL 188 G G+G+ T + +Y AG AT+E D +G +R +A A +AAE V +++ Sbjct: 108 TRNGRLGGDGNGTATGTVYQAGLSATYELDFWGRNRAVRDSARAGLRAAEFDRDTVHLTV 167 Query: 189 AAEVANAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQLDASR 248 A VA++++ ++R+A+ + +V + +++L + R G A+ LE + + L A Sbjct: 168 TAGVASSWLQTVGLRERVAIADLSVASAQRLLDVIESRARAGAANPLELAQQRGVLAAQM 227 Query: 249 ADLIPLQADREAYLNQLATLLGQEPGTLDNELTATAPVPLPPQQVAVGDPVTLLRRRPDV 308 ++ E L LA LLG+ P L L T L V G P LL RRPD+ Sbjct: 228 RARAAIRQQAEDSLTTLAVLLGRNPSLL--VLAGTELNKLQAPAVVAGLPADLLVRRPDI 285 Query: 309 RAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGAPMLSWNFL 368 AE L+ A I A AA P + L G I G S + D+ A L+ Sbjct: 286 ARAEAQLSGADANIIAARAAMLPRISLGGDITAGSNRVSSV--FDNPVYSLAAGLAAPIF 343 Query: 369 DFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSLSRFRYGRVTVATLARTKA--SA 426 + GR A A R+E YR+A++ A D E +L+ + +A+ + A Sbjct: 344 NAGRLAAGRDLALAQREELLANYRRAIVAAFGDVEVALNAV--AGIDAQRIAQEEELRQA 401 Query: 427 DRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGLGW 483 RA LA RY+AG T + LLD R AQ Q ++ V + KALG GW Sbjct: 402 QRAFNLAESRYRAGAETSLTLLDAQRTLYAAQDAAAQLRTLRLQAAVSLYKALGGGW 458 >gi|152981442|ref|YP_001353371.1| outer membrane protein, tolC-like [Janthinobacterium sp. Marseille] Length = 480 Score = 135 bits (340), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 123/363 (33%), Positives = 181/363 (49%), Gaps = 31/363 (8%) Query: 120 ARLPPANLGALTGGSSSGNGSATS--------ANLYSAGFDATWEADLFGAHRRAIQAAG 171 ARLP ++G GS N S+T+ +N S +A+WE DL+G +A A Sbjct: 97 ARLPTLSVG--LAGSRDSNPSSTTRSTTVQNPSNNVSLSGNASWEVDLWGRLAQATTGAQ 154 Query: 172 ATAQAAEASLADVQVSLAAEVANAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGT 231 A QA+ LA ++S A +A Y SLR +L++ E++V A ++ L LT R G Sbjct: 155 ANLQASVDDLAAARLSAQATLAQTYFSLRTTDAQLSVLERSVAAYQRSLDLTQFRYGGGV 214 Query: 232 ASSLEQIRIQNQLDASRADLIPLQADREAYLNQLATLLGQEPGTLDNELTATAP-VPLPP 290 + ++ ++ Q QL A++A L A R + +A LLG P L E A+ P PL P Sbjct: 215 VARVDVLQAQTQLKAAQAQLAEAIAQRAILEHAIAVLLGVPPSALALERNASLPQAPLVP 274 Query: 291 QQVAVGDPVTLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLT 350 Q + P TLL RRPD+ AA+R +AA A+IG A AA +PSL L GG S L+ Sbjct: 275 QML----PTTLLERRPDIAAAQRRVAAAYAQIGVAHAAYFPSLVLSAG---GGYQNSSLS 327 Query: 351 RLDDFAAIGAPMLSWNF--------LDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDA 402 L I AP L W+ D G+ + QA+ V D+A YRQ VL AL++ Sbjct: 328 NL-----ISAPNLFWSLGASVAQAIFDGGQRQLASAQAQSVADQATSAYRQTVLTALQEV 382 Query: 403 EDSLSRFRYGRVTVATLARTKASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLL 462 ED+L + + +A R LE+ +Y+AGT + + + + ++ NLL Sbjct: 383 EDNLILAAQLQQEAQLQQESLEAAQRTLEITLDQYRAGTVSYLNVAIAQTTALVSENNLL 442 Query: 463 QAE 465 + Sbjct: 443 STQ 445 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.316 0.128 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 833,122 Number of extensions: 28447 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 14 Length of query: 488 Length of database: 1,221,021 Length adjustment: 87 Effective length of query: 401 Effective length of database: 899,382 Effective search space: 360652182 Effective search space used: 360652182 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)