BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3003.p (430 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981547|ref|YP_001354871.1| major facilitator transporter [... 251 7e-68 gi|152981192|ref|YP_001353300.1| major facilitator transporter [... 76 3e-15 gi|152981201|ref|YP_001353559.1| major facilitator superfamily d... 55 8e-09 >gi|152981547|ref|YP_001354871.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 417 Score = 251 bits (640), Expect = 7e-68, Method: Compositional matrix adjust. Identities = 148/385 (38%), Positives = 234/385 (60%), Gaps = 5/385 (1%) Query: 29 LAFLQFAVILDFMLMSPLGALIMPAMRITPQQFGLVVSAYAFSAGASGLLTAGFADRFDR 88 +A +QF ILDFM+M PLG ++ + I QFGL++S+Y F+A AS LL A + DRFDR Sbjct: 20 IAGIQFTHILDFMIMMPLGPQLIRVLHIDTHQFGLLLSSYTFTAAASSLLAATYIDRFDR 79 Query: 89 KRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGR 148 ++L+L Y F++ T+ CGLA +F LL+AR + G FGG++G + + D+ RG+ Sbjct: 80 RKLMLVLYACFIIATLCCGLAPNFTFLLIARALAGAFGGILGGLVQTMVADVIPFERRGK 139 Query: 149 VMGIIQTSFAASQVLGIPIGLYLSNTWN---WHVPFLAMAGLGAVGGLVVSFKMQP-VDA 204 +G + +F+ S V G+P+GL L++T+ W PF + L + V+ +K+ P + A Sbjct: 140 ALGTVMAAFSISTVAGVPLGLLLASTFTTLGWRAPFFFIVLLAGIF-WVIGYKLLPNMTA 198 Query: 205 HLKLKQEHSAWMHLYHTIAEPRYLVAFATTALLMTGGFMLMPFSSAYLVGNLGIDLHHLP 264 HL K + + ++ EPR+L AFA A++M GF ++P+ + Y+ N+G+ + Sbjct: 199 HLHNKPLGNVFQRIFAVAREPRHLTAFAFIAMIMMAGFTVIPYIALYMTTNVGLPESFIT 258 Query: 265 TVYLITGLCTITFGPLIGRAADKLGKLRVFFFGALLSIIMVLIYTHLGPVTVPMIVLVNA 324 VYL G T LIGR ADK GKL+V+ AL S I +LI THL P+ ++++ + Sbjct: 259 VVYLCGGAATFFTSQLIGRMADKYGKLKVYRRVALASFIPLLITTHLVPIAWWLVLINST 318 Query: 325 VLFLGIFSRMIPFQALVSSVPAPTQRGSFNAVSASIQQLSGGVASVVAGQIVTLGADGQL 384 + F+ + RM+P A+VS+VP RG+F + +S+Q LS G+A+++AG I+T DG + Sbjct: 319 IFFILVPGRMVPGMAMVSAVPEAHVRGTFMTLISSVQMLSSGLATLIAGLIITRTPDGMI 378 Query: 385 LHFEVVGYVIVASTLLASVLVWRLN 409 H++ VGY+ VA ++ L RL Sbjct: 379 EHYDTVGYLAVACGVVTIWLAQRLR 403 >gi|152981192|ref|YP_001353300.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 390 Score = 76.3 bits (186), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 87/308 (28%), Positives = 140/308 (45%), Gaps = 24/308 (7%) Query: 28 LLAFLQFAV-ILDFMLMSPLGALIMPAMRITPQQFGLVVSAYAFSAGASGLLTAGFADRF 86 +LA FA+ +F++M L + ++ ++ G ++S YA + A Sbjct: 7 ILALSAFAIGTTEFVIMGLLPD-VARSLSVSIPSAGWLISGYALGVAIGAPIMAVATASL 65 Query: 87 DRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAIATDLFAPHLR 146 RKR LL G F++G V C +A ++ L++ARI+T L G I +A L + + Sbjct: 66 PRKRALLLLMGIFIVGNVLCAVATNYNFLMVARIITALCHGAFFGIGSVVAASLVPENRK 125 Query: 147 GRVMGIIQTSFAASQVLGIPIGLYLSNTWNWHVPFLAMAGLGAVGGLVVSFKMQPVDAHL 206 + ++ T + VLG+P+G L W +PF A+A LG + L+ +++ PV+ H Sbjct: 126 ASAVALMFTGLTLANVLGVPLGTALGQVAGWQMPFWAVAVLGVL-SLIGLWRVMPVNRH- 183 Query: 207 KLKQEHSAWMHLYHTIAEPR---YLVAFATTALLMTGGFMLMPFSSAYLVGNLGIDLHHL 263 +E + L I + R A +TT L F L + + L + H + Sbjct: 184 ---EEKT---DLRAEIGQLRNAGLWAALSTTMLFSAATFALFTYVAPLLEDVTHLSPHGV 237 Query: 264 PTVYLITGLCTITFGPLI-GRAAD-KLGKLRVFFFGALLSIIMVL-IYTHLGPVTVPMIV 320 + GL +T G I GR AD +LG AL I MVL + + L T P ++ Sbjct: 238 TWTLFLIGL-GLTVGNYIGGRLADWRLGT-------ALTGIFMVLAVVSSLVRWTSPSLI 289 Query: 321 LVNAVLFL 328 V LFL Sbjct: 290 PVEINLFL 297 >gi|152981201|ref|YP_001353559.1| major facilitator superfamily drug efflux antiporter [Janthinobacterium sp. Marseille] Length = 408 Score = 55.1 bits (131), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 45/140 (32%), Positives = 68/140 (48%), Gaps = 1/140 (0%) Query: 62 GLVVSAYAFSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIV 121 G V+A A + L+ FDRKR++L VL V A++ LL++R++ Sbjct: 69 GQAVTATALVGAGAALMIPVLTRNFDRKRVILTLTILLVLSNVLAATAENLPVLLVSRVL 128 Query: 122 TGLFGGVIGSITLAIATDLFAPHLRGRVMGIIQTSFAASQVLGIPIGLYLSNTWNWHVPF 181 G+ G S+ A+A L +L R M I T + + V PIG Y+ + W W F Sbjct: 129 LGIALGGFWSMAAALAMRLVPDNLFARAMSFILTGVSVATVGAAPIGAYMGDLWGWRSAF 188 Query: 182 LAMAGLGAVGGLVVSFKMQP 201 +A AG+ ++ LVV F P Sbjct: 189 IA-AGIVSIITLVVQFFAIP 207 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.328 0.142 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 875,024 Number of extensions: 35113 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 11 Length of query: 430 Length of database: 1,221,021 Length adjustment: 86 Effective length of query: 344 Effective length of database: 903,079 Effective search space: 310659176 Effective search space used: 310659176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)