BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2969.p (246 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981402|ref|YP_001352246.1| acetoacetyl-CoA reductase [Jant... 300 7e-83 gi|152981443|ref|YP_001353048.1| 3-oxoacyl-[acyl-carrier protein... 177 5e-46 gi|152981493|ref|YP_001353708.1| short-chain dehydrogenase [Jant... 130 1e-31 gi|152981530|ref|YP_001353550.1| short chain dehydrogenase/reduc... 125 3e-30 gi|152980322|ref|YP_001353173.1| 3-hydroxybutyrate dehydrogenase... 111 4e-26 >gi|152981402|ref|YP_001352246.1| acetoacetyl-CoA reductase [Janthinobacterium sp. Marseille] Length = 246 Score = 300 bits (767), Expect = 7e-83, Method: Compositional matrix adjust. Identities = 148/243 (60%), Positives = 183/243 (75%), Gaps = 1/243 (0%) Query: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEGNVS 63 R+A VTGGMGG+G AIC +L GY VV P + + D WL M+ GYD HA +VS Sbjct: 3 RLALVTGGMGGLGEAICIKLAALGYQVVTTYSPGNKKADEWLKLMKESGYDFHAYPCDVS 62 Query: 64 DWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVTKQV 123 D++S + ++ E+G VDVLVNNAGITRD F+KM K DWDAV+ TNL+S+FN+TK V Sbjct: 63 DYDSAQQCVAQIEKEMGPVDVLVNNAGITRDITFKKMGKPDWDAVMATNLDSVFNMTKPV 122 Query: 124 IEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSP 183 +GM ERG+GRIINISSVNGQKG FGQTNYS AKAG+HGFT ALA EVA K VTVNT+SP Sbjct: 123 CDGMVERGWGRIINISSVNGQKGAFGQTNYSAAKAGMHGFTKALALEVARKAVTVNTISP 182 Query: 184 GYIGTDMVRAIRPEVLE-KIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLNGG 242 GYIGT MV AI +VL+ KIV IP+ RLG+PEEIA +VA++AS E + TG++ ++NGG Sbjct: 183 GYIGTKMVMAIPQDVLDTKIVPQIPMGRLGKPEEIAGLVAYLASDEAAFLTGANIAINGG 242 Query: 243 LYM 245 +M Sbjct: 243 QHM 245 >gi|152981443|ref|YP_001353048.1| 3-oxoacyl-[acyl-carrier protein] reductase [Janthinobacterium sp. Marseille] Length = 247 Score = 177 bits (449), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 95/248 (38%), Positives = 149/248 (60%), Gaps = 10/248 (4%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEG 60 +S ++A VTG GIG AI L K G V+ S A Y + +G Sbjct: 6 LSGQVALVTGASRGIGRAIAQELAKLGAKVIGTATSES-------GAAAISEYLGNGGKG 58 Query: 61 ---NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLF 117 +V+D + A ++++ E G V +LVNNAGIT+D +M +WD+VI TNL ++ Sbjct: 59 VVLDVNDAARSVALIDEIQKEFGAVSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVG 118 Query: 118 NVTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVT 177 +++ V+ GM + GRIINI+SV G G GQ NY+ AKAG+ G + ALA+E+ ++ +T Sbjct: 119 RLSRAVLRGMMKAKTGRIINITSVVGSSGNAGQMNYAAAKAGVAGMSRALAREIGSRNIT 178 Query: 178 VNTVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDF 237 VN ++PG+I TDM + + + + ++ +P+ RLG PE+IA+ V+++AS + Y TG+ Sbjct: 179 VNCIAPGFIDTDMTKTLSEQQIAALLQQVPLGRLGVPEDIAAAVSFLASPQASYITGTTL 238 Query: 238 SLNGGLYM 245 +NGG+YM Sbjct: 239 HVNGGMYM 246 >gi|152981493|ref|YP_001353708.1| short-chain dehydrogenase [Janthinobacterium sp. Marseille] Length = 246 Score = 130 bits (326), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 71/243 (29%), Positives = 134/243 (55%), Gaps = 3/243 (1%) Query: 2 SKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEGN 61 +K++A +TG GIG AI RL +DG+ VV ++T D +A ++A ++ A + + Sbjct: 5 TKKVAIITGASRGIGAAIAERLAQDGFAVVINYASSATEADALVAKLQAAQHEAIAVKAD 64 Query: 62 VSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVTK 121 V++ + F++ ++G+VDVL+NNAGI + + S ++ N+ FN + Sbjct: 65 VANSADVRRLFDETEQKLGKVDVLINNAGILQMASLAETSDELFERTFSINVRGTFNTLR 124 Query: 122 QVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTV 181 + + + GR++N SS + Y+ +KA + T A+E+ + +TVN V Sbjct: 125 EAATRLNDG--GRVVNFSSTTVAMSLPNYSVYTGSKAAVEVLTPIFAKEMRGRNITVNAV 182 Query: 182 SPGYIGTDMVRAIRPEV-LEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLN 240 +PG + TD+ + E ++++ + P++RLG+P++IA+ V+++ +GG+ G N Sbjct: 183 APGPVATDLFFNGKTEAQIQQLANMPPLQRLGQPDDIAAAVSFLVGRDGGWINGQVLRAN 242 Query: 241 GGL 243 GGL Sbjct: 243 GGL 245 >gi|152981530|ref|YP_001353550.1| short chain dehydrogenase/reductase family oxidoreductase [Janthinobacterium sp. Marseille] Length = 249 Score = 125 bits (313), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 81/244 (33%), Positives = 125/244 (51%), Gaps = 4/244 (1%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEG 60 ++ ++A+V GG GIG AI RL +DG V ++ + + A G A Sbjct: 7 LTGKVAFVQGGSRGIGAAIVKRLARDGAAVAFTYVSSADKAKAVAQEIEAAGGRALAIHA 66 Query: 61 NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVT 120 + SD + + A + G +D+LVNNAG+ D+D + N+ S+F T Sbjct: 67 DSSDASAVQQAIQAAAKAFGRIDILVNNAGVLALAPVEDFKLEDFDRTVAVNVRSVFVAT 126 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQF-GQTNYSTAKAGIHGFTMALAQEVATKGVTVN 179 ++ M E GRII I S N + F G Y+ +KA I G T LA+++ +G+TVN Sbjct: 127 QEAARHMGEG--GRIITIGSTNADRMPFAGGAVYAMSKAAIVGLTKGLARDLGPRGITVN 184 Query: 180 TVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSL 239 V PG + TDM A + + + + + R G+ EEIAS VA++A E GY TG+ ++ Sbjct: 185 NVQPGPVDTDMNPA-NSDFADTLKGLMAIGRYGKDEEIASFVAYLAGPEAGYITGASLTI 243 Query: 240 NGGL 243 +GG Sbjct: 244 DGGF 247 >gi|152980322|ref|YP_001353173.1| 3-hydroxybutyrate dehydrogenase [Janthinobacterium sp. Marseille] Length = 264 Score = 111 bits (278), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 78/252 (30%), Positives = 119/252 (47%), Gaps = 13/252 (5%) Query: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVA-GCGPNSTRKDTWLAAMRAHGYDIHASEGNV 62 + A VTG GIG I L + G +V G G + K A MR +G ++ Sbjct: 9 KTALVTGSTSGIGLGIAQALAQQGADIVLNGFGDANEIKTLQAALMRNYGVKCVHHPADM 68 Query: 63 SDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVTKQ 122 S +A + G VD+LVNNAGI WDA+I NL+S F+ T+ Sbjct: 69 SKPAEIEAMMAFAAQQFGRVDILVNNAGIQHTETIEDFPVEKWDAIIAINLSSAFHTTRL 128 Query: 123 VIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVS 182 + M + +GRIINI+SV+G G ++ Y AK GI G T A E+A G+T N + Sbjct: 129 ALPMMQAKNWGRIINIASVHGLVGSVQKSAYVAAKHGIVGLTKVTALEMAHTGITCNAIC 188 Query: 183 PGYIGTDMV------RAIRP----EVLEK--IVSGIPVKRLGEPEEIASIVAWIASAEGG 230 PG++ T +V RA R E +K + P P+++ ++ ++ S Sbjct: 189 PGWVLTPLVQKQVDARAAREGLSDEAAKKDLVSEKQPSGEFVTPQQLGALAVFLCSEAAV 248 Query: 231 YATGSDFSLNGG 242 G+ ++++GG Sbjct: 249 QVRGAAWNMDGG 260 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.316 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 618,401 Number of extensions: 24984 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 21 Length of query: 246 Length of database: 1,221,021 Length adjustment: 81 Effective length of query: 165 Effective length of database: 921,564 Effective search space: 152058060 Effective search space used: 152058060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)