BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2967.p (458 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981113|ref|YP_001353088.1| MiaB-like tRNA modifying enzyme... 825 0.0 gi|152979971|ref|YP_001352121.1| tRNA modifying enzyme [Janthino... 149 5e-37 >gi|152981113|ref|YP_001353088.1| MiaB-like tRNA modifying enzyme [Janthinobacterium sp. Marseille] Length = 453 Score = 825 bits (2131), Expect = 0.0, Method: Compositional matrix adjust. Identities = 405/452 (89%), Positives = 432/452 (95%) Query: 6 TPASPALPKVGFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGFIDAAV 65 T A A PK+GFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGFIDAAV Sbjct: 2 TNALQATPKIGFVSLGCPKALVDSEQILTQLRAEGYDTAKSYDGADLVIVNTCGFIDAAV 61 Query: 66 QESLDAIGEALNENGKVIVTGCLGAKKDAEGEDIIQKIHPKVLEVTGPHAVGEVMQAVHK 125 QESLDAIGEAL+ENGKVIVTGCLGAKKDA+G+DIIQK+HPKVL VTGPHA+GEVM AVHK Sbjct: 62 QESLDAIGEALHENGKVIVTGCLGAKKDADGDDIIQKVHPKVLAVTGPHALGEVMDAVHK 121 Query: 126 HLPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIADVML 185 H+PKPHAPFIDLVP QG+KLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIADVM+ Sbjct: 122 HMPKPHAPFIDLVPAQGIKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIADVMM 181 Query: 186 EAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPLKTHMTQLVGALGELAQQYGAW 245 EAENLFKAGVKELLVISQDTSAYGVDVKFR GFWNGKP+KTHMTQLV ALGELA+QYGAW Sbjct: 182 EAENLFKAGVKELLVISQDTSAYGVDVKFRMGFWNGKPVKTHMTQLVEALGELAKQYGAW 241 Query: 246 VRLHYVYPYPHVDQIIPLMAEGKILPYLDVPLQHAHPDVLKRMKRPANGEKNIERIQAWR 305 VRLHYVYPYPHVD IIP+MAEGKILPYLDVPLQHAHPDVLKRMKRPA+GEKNIERIQAWR Sbjct: 242 VRLHYVYPYPHVDAIIPMMAEGKILPYLDVPLQHAHPDVLKRMKRPASGEKNIERIQAWR 301 Query: 306 AMCPDLTIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANEIANPVP 365 AMCPDLTIRSTFIAGFPGET+AEFEYLLDFLKEAEIDRLGCFAYSPVEGATAN++ N VP Sbjct: 302 AMCPDLTIRSTFIAGFPGETDAEFEYLLDFLKEAEIDRLGCFAYSPVEGATANDLPNAVP 361 Query: 366 EEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPEIDGVVYV 425 EEVREERRGRVM LQEEISKKRLQAKVGKT+RVL+DEV+RNGG R+ +DAPEIDGVVYV Sbjct: 362 EEVREERRGRVMLLQEEISKKRLQAKVGKTMRVLLDEVNRNGGVARSGADAPEIDGVVYV 421 Query: 426 KPPFEPHRKLEVGQFVDVRISAADAHDLWGAA 457 KPP+EPH KL+VG+F+DV+I+ ADAHDLW A Sbjct: 422 KPPYEPHLKLKVGEFIDVKITGADAHDLWAEA 453 >gi|152979971|ref|YP_001352121.1| tRNA modifying enzyme [Janthinobacterium sp. Marseille] Length = 446 Score = 149 bits (375), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 123/461 (26%), Positives = 213/461 (46%), Gaps = 43/461 (9%) Query: 14 KVGFVSLGCPKALVDSEQILTQLRA-EGYDTAKSYDGADLVIVNTCGFIDAAVQESLDAI 72 K+ + GC DS++++ L A EG + + AD++++NTC + A ++ + Sbjct: 4 KIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVFSDL 63 Query: 73 GE--ALNENGKVIVTGCLGAKKDAEGEDIIQKIHPKVLEVTGPHAVGEVMQAVHKH---- 126 G L N +V G G EG+ I+++ P V V GP + + + + + Sbjct: 64 GRLRELKFNNPDLVIGVGGCVASQEGDAIVKR-APYVDLVFGPQTLHRLPEMLKQRRSTG 122 Query: 127 -----LPKPHAPFIDLVPPQGVKLTPKHFAYLKISEGCNHRCSFCIIPSMRGDLVSRPIA 181 + P D +PP V+ A++ I EGC+ CS+C++P RG+ VSR Sbjct: 123 RSQVDISFPEIEKFDHMPPAKVEGAT---AFVSIMEGCSKYCSYCVVPYTRGEEVSRRFE 179 Query: 182 DVMLEAENLFKAGVKELLVISQDTSAYGVDVKFRTGFWNGKPLKTHMTQLVGALGELAQQ 241 DV+ E L GVKE+ ++ Q+ +AY R +G+ L+ + EL Sbjct: 180 DVLAEVAGLEAQGVKEITLLGQNVNAY------RGEMADGEI--ADFALLIEYIAELE-- 229 Query: 242 YGAWVRLHYVYPYPH--VDQIIPLMAEG-KILPYLDVPLQHAHPDVLKRMKRPANGEKNI 298 R+ +V +P ++I A+ K++ +L +P QH +L MKR + Sbjct: 230 --GIERIRFVTSHPKEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYK 287 Query: 299 ERIQAWRAMCPDLTIRSTFIAGFPGETEAEFEYLLDFLKEAEIDRLGCFAYSPVEGATAN 358 ++ R + P+++I S FI GFPGET+A+FE ++ + + D F +SP G A Sbjct: 288 SILRRLREVRPNISISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAA 347 Query: 359 EIANPVPEEVREERRGRVMQLQEEISKKRLQAKVGKTLRVLIDEVDRNGGTGRTYSDAPE 418 + + P EV+ +R R+ + ++ +++ VG R+L++ G + D E Sbjct: 348 NLEDDTPHEVKLQRLQRLQAVIDQNTRRYSDEMVGTVQRILVE--------GPSKKDPDE 399 Query: 419 IDGVV----YVKPPFEPHRKLEVGQFVDVRISAADAHDLWG 455 + G V H +GQ VDV I + A+ L G Sbjct: 400 LQGRTENNRVVNFAAGEHGARLIGQMVDVNIVQSFAYTLRG 440 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,090,255 Number of extensions: 46031 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 2 Length of query: 458 Length of database: 1,221,021 Length adjustment: 86 Effective length of query: 372 Effective length of database: 903,079 Effective search space: 335945388 Effective search space used: 335945388 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)