BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2921.p (447 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981185|ref|YP_001353813.1| GTP-binding protein EngA [Janth... 805 0.0 gi|152981181|ref|YP_001355383.1| tRNA modification GTPase TrmE [... 72 9e-14 gi|152981217|ref|YP_001353061.1| GTP-binding protein Era [Janthi... 62 7e-11 >gi|152981185|ref|YP_001353813.1| GTP-binding protein EngA [Janthinobacterium sp. Marseille] Length = 447 Score = 805 bits (2080), Expect = 0.0, Method: Compositional matrix adjust. Identities = 390/449 (86%), Positives = 424/449 (94%), Gaps = 4/449 (0%) Query: 1 MKPVIALVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGEGRVGERPFLVIDTGGF 60 MKPVIAL+GRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGEGRVGERPFLVIDTGGF Sbjct: 1 MKPVIALIGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGEGRVGERPFLVIDTGGF 60 Query: 61 EPVAKDGIMYEMAKQTKQAVVEADIVVFIVDGRQGLTPHDKTITDFLRKCGRSVLLVVNK 120 EPVAK+GIM+EMAKQTKQAV EAD+V+FIVDGRQGLTPHDKTITDFLRK GRSV+LVVNK Sbjct: 61 EPVAKEGIMHEMAKQTKQAVAEADVVIFIVDGRQGLTPHDKTITDFLRKSGRSVMLVVNK 120 Query: 121 SEGMKYTSVTADFYELGMGDPYVISAAHGDGVADLVQEALDMVEAERAQEPEPEEPAV-- 178 +EGMKYT+VTADFYELGMGDPYVISAAHGDGV DLV+EAL++ A+R PE E PA Sbjct: 121 AEGMKYTTVTADFYELGMGDPYVISAAHGDGVNDLVEEALNIAFAQRP--PEEEAPASND 178 Query: 179 RGTKIAIVGRPNVGKSTLVNTLLGEERVIAFDMPGTTRDSIEIPFEREGRHYTLIDTAGI 238 R K+AIVGRPNVGKSTLVNTLLGEERVIAFD+PGTTRDSIEIPFER+G+HYTLIDTAGI Sbjct: 179 RSIKLAIVGRPNVGKSTLVNTLLGEERVIAFDLPGTTRDSIEIPFERDGKHYTLIDTAGI 238 Query: 239 RRRGKVFEAIEKFSVVKTLKSISDANVVLLLLDAQQDISEQDAHIAGFVLESGRALVVGV 298 RRRGKVFEAIEKFSVVKTL+SIS+ANVVLLLLDAQQDISEQDAHIAGF+LESGRALVVGV Sbjct: 239 RRRGKVFEAIEKFSVVKTLQSISEANVVLLLLDAQQDISEQDAHIAGFILESGRALVVGV 298 Query: 299 NKWDGMESDQRNQIKMDLERKLNFLSFAKFHFISALKSTGIGPLMKSIDSAYAAAMAKLS 358 NKWDG+ SD+R++IKMDLERKL+FLSFAK HF+SALKS+GIGP+MKS+D AYAAAM+ LS Sbjct: 299 NKWDGLTSDRRDEIKMDLERKLSFLSFAKTHFVSALKSSGIGPMMKSVDGAYAAAMSNLS 358 Query: 359 TPRLTRALEEALEHQQPRRKGSIRPKLRYAHQGGQNPPVVVIHGNALEAIDDNYKRYLEK 418 TP+LTRAL EA+EHQQPRRKGSIRPKLRYAHQGG NPP+VVIHGNALEAID NYKR+LEK Sbjct: 359 TPKLTRALIEAVEHQQPRRKGSIRPKLRYAHQGGMNPPIVVIHGNALEAIDANYKRFLEK 418 Query: 419 HFRETFSLVGTPLRIEFKSGKNPFIRQEK 447 HFRETFSLVGTPLRIE +SGKNPF R EK Sbjct: 419 HFRETFSLVGTPLRIELRSGKNPFSRSEK 447 >gi|152981181|ref|YP_001355383.1| tRNA modification GTPase TrmE [Janthinobacterium sp. Marseille] Length = 465 Score = 71.6 bits (174), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 37/104 (35%), Positives = 59/104 (56%) Query: 180 GTKIAIVGRPNVGKSTLVNTLLGEERVIAFDMPGTTRDSIEIPFEREGRHYTLIDTAGIR 239 G I + G+PNVGKS+L+N L G + I + GTTRD + + EG +IDTAGIR Sbjct: 223 GLNIVLAGQPNVGKSSLLNALAGSDVAIVTAIAGTTRDKVIETIQIEGIPVNVIDTAGIR 282 Query: 240 RRGKVFEAIEKFSVVKTLKSISDANVVLLLLDAQQDISEQDAHI 283 + +E+ + +T ++ A+V++ +LDA + + D I Sbjct: 283 DASDATDEVERIGIERTWAAVKTADVIIHMLDANRGPTRADEQI 326 Score = 56.6 bits (135), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 37/103 (35%), Positives = 55/103 (53%), Gaps = 2/103 (1%) Query: 5 IALVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGEGRVGERPFLVIDTGGFEPV- 63 I L G+PNVGKS+L N L S A+V + G TRD+ ++ P VIDT G Sbjct: 226 IVLAGQPNVGKSSLLNALAGSDVAIVTAIAGTTRDKVIETIQIEGIPVNVIDTAGIRDAS 285 Query: 64 -AKDGIMYEMAKQTKQAVVEADIVVFIVDGRQGLTPHDKTITD 105 A D + ++T AV AD+++ ++D +G T D+ I + Sbjct: 286 DATDEVERIGIERTWAAVKTADVIIHMLDANRGPTRADEQIVE 328 >gi|152981217|ref|YP_001353061.1| GTP-binding protein Era [Janthinobacterium sp. Marseille] Length = 300 Score = 62.0 bits (149), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 38/129 (29%), Positives = 68/129 (52%), Gaps = 2/129 (1%) Query: 5 IALVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGEGRVGERPFLVIDTGGFEPVA 64 IA+VGRPNVGKSTL N L ++ ++ + TR R G V + F+ +DT GF+ Sbjct: 15 IAIVGRPNVGKSTLMNELIGAKVSITSRKAQTTRHRITGIQTVEDAQFVYVDTPGFQTRH 74 Query: 65 KDGIMYEMAKQTKQAVVEADIVVFIVDGRQGLTPHDKTITDFLRKCGRSVLLVVNKSEGM 124 + + + + + AD+++++++ D+ + D + K +LV+NKS+ + Sbjct: 75 SNALNKTLNRTVTNTLTAADVILYVIEAGT-FGQADQQVLDLIPK-NVPCILVINKSDRV 132 Query: 125 KYTSVTADF 133 K +V F Sbjct: 133 KDKAVLLPF 141 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 999,156 Number of extensions: 40629 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 5 Length of query: 447 Length of database: 1,221,021 Length adjustment: 86 Effective length of query: 361 Effective length of database: 903,079 Effective search space: 326011519 Effective search space used: 326011519 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)