BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2746.p (524 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982589|ref|YP_001352814.1| major facilitator superfamily m... 608 e-175 gi|152980367|ref|YP_001352036.1| major facilitator superfamily d... 138 6e-34 gi|152982526|ref|YP_001355122.1| major facilitator superfamily d... 130 2e-31 gi|152982183|ref|YP_001354785.1| major facilitator transporter [... 99 5e-22 gi|152982562|ref|YP_001352191.1| major facilitator superfamily d... 98 1e-21 >gi|152982589|ref|YP_001352814.1| major facilitator superfamily multidrug resistance B translocase [Janthinobacterium sp. Marseille] Length = 517 Score = 608 bits (1568), Expect = e-175, Method: Compositional matrix adjust. Identities = 299/503 (59%), Positives = 399/503 (79%), Gaps = 1/503 (0%) Query: 10 PYTPPPPLSGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFA 69 P + PPLSG L++GTL++SLAVFMNVLDSSIANV+IP ISG+LGVS +GTWVITSF+ Sbjct: 5 PRSVVPPLSGTPLIVGTLALSLAVFMNVLDSSIANVSIPAISGDLGVSPQQGTWVITSFS 64 Query: 70 AANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPM 129 ANAIS+PLTGWLTQR+G VRLF+ S+LLFVLAS LCG AP++ IL+ ARVLQG VAGPM Sbjct: 65 VANAISVPLTGWLTQRIGQVRLFIGSILLFVLASCLCGFAPSIEILIAARVLQGAVAGPM 124 Query: 130 VPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGL 189 +PLSQ+LLL +YP +KS ALA WGMT +VAP+ GP LGGWI+D+YTWPWIF+INIP+GL Sbjct: 125 IPLSQALLLSSYPPAKSGMALAFWGMTTLVAPIMGPLLGGWISDNYTWPWIFFINIPIGL 184 Query: 190 IAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGL 249 AA T +IY+ RE+ T+KLPIDK+GL LLV WV + Q+MLDKGK+LDWF S I+ L Sbjct: 185 FAAWATWSIYKERESRTYKLPIDKVGLVLLVLWVGSLQLMLDKGKELDWFHSGEIIILAS 244 Query: 250 IALVAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYL 309 +A+VAF YFV+WE+ +HPVVDL LF RNF+GG IAIS+ YG+FFG+LVILP WLQ + Sbjct: 245 VAVVAFIYFVIWEIGEEHPVVDLSLFKGRNFSGGVIAISVAYGLFFGSLVILPLWLQTQI 304 Query: 310 GYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVD 369 GY + ++G AP+GIFA+IL P++G+ LP +DAR VAT +F+ F++VF++RS++ VD Sbjct: 305 GYTATEAGKVMAPVGIFAIILSPIVGKMLPKIDARWVATTAFLIFSLVFFMRSQFTQDVD 364 Query: 370 QMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTA 429 MT+++PT++QG AMFF+PLT ++LSG P++IPAAAGLS+FVR+ G +GTS+ +T Sbjct: 365 MMTLMIPTVIQGAAMAMFFIPLTSIILSGQAPDKIPAAAGLSNFVRIMFGGIGTSVMSTM 424 Query: 430 WNNRTILHHAQLTEHATPYSPWLQESVNNISSTLGLNAEQTAGMLERTITFQAAMLGIND 489 W+NRTILHHAQL E+ ++P ++V+ + S G++ + +++R +T QA+ L D Sbjct: 425 WDNRTILHHAQLAEYTGAHNPAFGQAVSALMSR-GMSEQAAWAVIDRQMTVQASTLAATD 483 Query: 490 IFYVSAVIFIVIIPLIWIIKPSK 512 +F++SA++F+ +I IWI K SK Sbjct: 484 LFWMSAIMFLCLIGFIWITKRSK 506 >gi|152980367|ref|YP_001352036.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 478 Score = 138 bits (348), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 87/256 (33%), Positives = 145/256 (56%), Gaps = 10/256 (3%) Query: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 V++A+FM LDS+I N AIP+++ +L V+ V++S+ A A+SIP++GW+ R G Sbjct: 24 VAIALFMEQLDSTIINTAIPSMAASLHVTPLSLKAVVSSYILALAVSIPISGWMADRFGT 83 Query: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 R+F +V LF +S LCGL+ +P+L+ AR+LQG A M+P+ + ++ +PKS+ Sbjct: 84 RRVFSVAVGLFTFSSILCGLSVNVPMLVAARILQGFSAAMMMPVGRLTVIRTFPKSELLA 143 Query: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK 208 A+ + A++ P+ GP +GG I W IF++N+P+GL A + R P ++ Sbjct: 144 AMNFVIIPALIGPLLGPTVGGLIVHWLPWQAIFFVNVPIGLAAQWMI-----HRHMPDYR 198 Query: 209 ----LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVWELN 264 P+D IGL L + VA +L+ + A+ T + L +I+L +++ Sbjct: 199 SDSPRPLDVIGLVLFGSGVALLSWILEIFGEHYLDATSTTI-LFIISLALLGAYILHARR 257 Query: 265 NDHPVVDLRLFGRRNF 280 HP++ L LF R F Sbjct: 258 TLHPLLQLTLFKTRTF 273 >gi|152982526|ref|YP_001355122.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 481 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 111/408 (27%), Positives = 193/408 (47%), Gaps = 10/408 (2%) Query: 37 VLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSV 96 +L S+I NVAIP +S + + WV T F A +S+ LT WL R G R + ++ Sbjct: 33 ILSSTIVNVAIPDLSRYFVLGQERAQWVSTGFMLAMTLSMLLTPWLLLRFGLRRTYGGAI 92 Query: 97 LLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMT 156 L ++ + G + P+LL RVL+GL +G M P+ ++L A+ + + A+ ++G Sbjct: 93 CLLLIGGVVGGFSINYPMLLSMRVLEGLASGVMQPIPAIVILRAFDQHEQGKAMGIFGFG 152 Query: 157 AVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRE-TPTHKLPIDKIG 215 V+AP GP++GG++ + + W IF++ +P L+ + S P P+D G Sbjct: 153 VVLAPAVGPSVGGFLVELFGWRSIFFVVVPPCLVVLVLIQRFLASYTPVPDSDKPLDWKG 212 Query: 216 LALL-VTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVWELNND-HPVVDLR 273 L L+ V+ V+ +++ D+ P V L +I + F++++ P+++++ Sbjct: 213 LLLIGVSIVSLLNGVVELRGDM-----PQAVVLLVIGVATLIGFILYQTRTSAEPLMNMQ 267 Query: 274 LFGRRNFA-GGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIFAVILMP 332 LF R FA G +A G G+ FG+ +LP ++Q + Y +GL P GI + +P Sbjct: 268 LFRHRQFAMGALVAFIYGMGL-FGSTYLLPIYMQMGMNYAPSQAGLVLLPAGIVLAVTIP 326 Query: 333 LIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPLT 392 L GR + A F L + + + ++ ++ I L+ Sbjct: 327 LAGRLADRYPPNVLVAIGLFLLAASFALMANGSPATSYLALMAWAVIGRIGLGFVLPALS 386 Query: 393 VLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRTILHHAQ 440 + + GL + A +FVR GA+G S+ R LH AQ Sbjct: 387 LGAMRGLDIGLVAQGASAINFVRQLGGAIGVSLVGVVLEWRIALHQAQ 434 >gi|152982183|ref|YP_001354785.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 469 Score = 99.4 bits (246), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 103/407 (25%), Positives = 176/407 (43%), Gaps = 20/407 (4%) Query: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 +SV++ V M LD++IAN A+P ++ L + + W++ + A ++ L + L Sbjct: 34 VSVAIGVGMASLDTAIANTALPAMAMQLNATPADSVWIVNIYQLAMVATLLPFAALGEIL 93 Query: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 G R+ + +LLF AS C A +LP L+ AR QG+ A ++ ++ +LL YP Sbjct: 94 GYRRIAIFGLLLFTAASLACAYAWSLPTLVIARFFQGVGASAIMGVNTALLRSIYPARML 153 Query: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPT 206 L + VA GP I ++WPW+F IN+P+GL A + + + T Sbjct: 154 GRGLGTNALVVAVAFAVGPTTASLILSVWSWPWLFAINVPLGLFAYFLARKVLPHTKRAT 213 Query: 207 HKLP-----IDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVW 261 HK + G LL+ + + +G L + + G ALV F + Sbjct: 214 HKFDALTAIYNAGGFGLLI-------LAIGEGAHLSDAKTVALELFG--ALVFFILLLRR 264 Query: 262 ELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTA 321 + + P++ + LF R F + + V LP + + LG +I++G Sbjct: 265 QKGHAAPMLPVDLFRRPLFTLSALTAVCTFAAQSLAFVSLPFYFETVLGRSAIETGFLIT 324 Query: 322 PIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNL---QVDQMTVILPTL 378 P + I+ P+ GR L D +G +++ L V + Sbjct: 325 PWAVMVGIMAPIAGR---LSDHYPPGMLGGVGLSVLSAGMVSLILMSPDVSVFGICWRMA 381 Query: 379 LQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSI 425 L GI F P ++S PPER +A+G+ + R+ A G ++ Sbjct: 382 LCGIGFGFFQAPNLRAIMSSAPPERGGSASGVVATSRLLGQASGAAL 428 >gi|152982562|ref|YP_001352191.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 518 Score = 97.8 bits (242), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 87/333 (26%), Positives = 159/333 (47%), Gaps = 9/333 (2%) Query: 10 PYTPPPPLSGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFA 69 +T P S LVL +SV+L + V+D ++ A+P ++ +L S W++ +A Sbjct: 10 KHTSTLPSSSRWLVLAIVSVAL--LLIVVDMTVLYAALPRLTADLTASASAKLWIVNIYA 67 Query: 70 AANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPM 129 + + G L RLG LF+ +++F +AS +P+ L+ +R L + A M Sbjct: 68 LTVSGLLLGMGTLGDRLGHKSLFIAGLVVFGIASLCAAYSPSSATLIASRALLAVGAAMM 127 Query: 130 VPLSQSLLLGAY-PKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVG 188 +P + S++ A+ +++ + A+ +W A GP +GG + + + W +F IN+P+ Sbjct: 128 MPATLSIIRLAFADENERAVAIGIWASVAFGGAALGPVVGGLLLEYFWWGSVFLINVPIV 187 Query: 189 LIAAGVTLAIYRSRETPTHKLPIDKIG-LALLVTWVAAFQIMLDKGK-DLDWFASPTIVT 246 L+A + LA Y + P D IG L ++ V + + GK + W + ++ Sbjct: 188 LVALPIALA-YIPKGKANKTRPWDLIGSLQIMAGLVGVTYALKEAGKREPAWGIALLVLL 246 Query: 247 LGLIALVAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQ 306 +G+ L FA + ++ HP++D LF F+ A + G ++ Q LQ Sbjct: 247 VGIFFLAVFARR---QQHSAHPMIDFSLFKNTIFSSSVAAAVVAAAALLGMELVFTQRLQ 303 Query: 307 QYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLP 339 L + +G+ P+ + A + PL GR LP Sbjct: 304 LVLDMTPLQAGVFILPLSLAAFVAGPLTGRVLP 336 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.324 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,144,871 Number of extensions: 49734 Number of successful extensions: 281 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 11 Length of query: 524 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 436 Effective length of database: 895,685 Effective search space: 390518660 Effective search space used: 390518660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)