BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2716.p (164 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980455|ref|YP_001351966.1| acyl-CoA thioester hydrolase [J... 78 4e-16 >gi|152980455|ref|YP_001351966.1| acyl-CoA thioester hydrolase [Janthinobacterium sp. Marseille] Length = 138 Score = 77.8 bits (190), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 43/125 (34%), Positives = 69/125 (55%), Gaps = 2/125 (1%) Query: 2 DLPSHQL-TMTVLMTPDMANFAGNVHGGTILKLLDQVAYACGSRYAGQYVVTLSVDQVMF 60 DLP+ ++ M V+ P AN G+V GG I+ +D C +R A VVT++V+ +F Sbjct: 8 DLPAGKIPQMRVVPMPHDANMHGDVFGGWIMAQVDLAGAVCATRRANGRVVTIAVNSFLF 67 Query: 61 RQPIHVGELVTFLASVNHTGKSSMEIGIKVVAENIRTQ-ETRHVNSCFFTMVAVDDQRKP 119 + P+ VG+L++ A + G +S+ + ++ AE R + E V T VA +D RKP Sbjct: 68 KHPVFVGDLLSIYAEIVKVGNTSVTVNVECYAERNRLEVEIVKVTEATLTYVATNDDRKP 127 Query: 120 IPVPP 124 +PP Sbjct: 128 RQLPP 132 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 344,984 Number of extensions: 12006 Number of successful extensions: 30 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 29 Number of HSP's successfully gapped: 1 Length of query: 164 Length of database: 1,221,021 Length adjustment: 76 Effective length of query: 88 Effective length of database: 940,049 Effective search space: 82724312 Effective search space used: 82724312 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)