BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2545.p (332 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Ja... 128 6e-31 gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthino... 108 3e-25 gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthi... 100 2e-22 gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid... 97 1e-21 >gi|152980390|ref|YP_001352411.1| 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 327 Score = 128 bits (321), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 83/253 (32%), Positives = 132/253 (52%), Gaps = 18/253 (7%) Query: 42 QLAQGCEAVAAFVNDDLGAAVLERLAAQGVRFITTRSTGFNHIDTQAARRLGISVARVAD 101 Q QG +AV ++ + A V+ A ++ I + G+N+ID AA G+ V D Sbjct: 41 QKMQGKDAVVVTASEKMSADVIA--ANPQLKAICNVAVGYNNIDVAAATAAGVMVTNTPD 98 Query: 102 YSPYSVAEFAVGLLLAVNRKIARASMRTREGNFE---LEGLMGFDLHGKTVGVIGTGKIG 158 + A++A LL+A R++ + R GN++ + +G D+HG T+G++G G+IG Sbjct: 99 VLNETTADYAWTLLMANARRVCESEHWLRAGNWKHWRFDSFLGADIHGATLGILGMGRIG 158 Query: 159 ALFARIMAGFGCTLLGSDMYQN-----PVFEALGG--RYVDKQALFAASDVISLHCPLTE 211 AR GF ++ Y N P EA RYV K+ L ++D + L P + Sbjct: 159 QAIARRSTGFDMNVI----YHNRSRATPEQEAYANNARYVSKEELLRSADHLILVLPYSP 214 Query: 212 ATRYIVNAESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQLRGLAIDVYEQEASLF-- 269 T +I+ A LA KP LVN +RGG+VD A+IAAL+ ++ +DV+E E +L Sbjct: 215 QTHHIIGAAELALMKPTATLVNIARGGIVDDVALIAALRENRIASAGLDVFENEPALHPD 274 Query: 270 FQDLSSTIITDDV 282 F LS+ ++T + Sbjct: 275 FLTLSNVVLTPHI 287 >gi|152982514|ref|YP_001355351.1| formate dehydrogenase [Janthinobacterium sp. Marseille] Length = 400 Score = 108 bits (271), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 76/246 (30%), Positives = 126/246 (51%), Gaps = 9/246 (3%) Query: 25 NTHELVFLQDRLDSSSAQLAQGCEAVAAFVNDDLGAAVL--ERLA-AQGVRFITTRSTGF 81 N H LV + D + + L + ++ A + ER+A A+ ++ I T G Sbjct: 67 NGHTLVVTSSK-DGADSVLDKELHDAEIIISQPFWPAYMTAERIAKAKKLKMIVTAGIGS 125 Query: 82 NHIDTQAARRLGISVARVADYSPYSVAEFAVGLLLAVNRKIARASMRTREGNFELEGLM- 140 +H D +AA + I+VA V + +SVAE V ++L+ R + + G + + + Sbjct: 126 DHTDLEAANKHNITVAEVTYCNSHSVAEHVVMMILSQVRNYIPSYNQVINGGWNIADCVE 185 Query: 141 -GFDLHGKTVGVIGTGKIGALFARIMAGFGCTLLGSDMYQNP--VFEALGGRYVDK-QAL 196 +DL +VG + G+IG R++ F L D ++ P V + L Y +L Sbjct: 186 RSYDLEAMSVGTVAAGRIGLRVLRLLKPFDVKLHYMDRHRLPEAVEKELNLTYHSTLDSL 245 Query: 197 FAASDVISLHCPLTEATRYIVNAESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQLRG 256 DV++L+CPL T +++N ++L K G ++NT+RG L D +A++AALKSGQL G Sbjct: 246 TKVCDVVTLNCPLHPETEHMINEKTLKNFKRGSYIINTARGKLCDRDAIVAALKSGQLAG 305 Query: 257 LAIDVY 262 A DV+ Sbjct: 306 YAGDVW 311 >gi|152982997|ref|YP_001354733.1| glycerate dehydrogenase [Janthinobacterium sp. Marseille] Length = 336 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 73/226 (32%), Positives = 111/226 (49%), Gaps = 6/226 (2%) Query: 44 AQGCEAVAAFVNDDLGAAVLERLAAQGVRFITTRSTGFNHIDTQAARRLGISVARVADYS 103 ++ +AV AF+ D + + L A ++ + G+++ D A + G+ V D Sbjct: 43 SKDADAVMAFMPDSVDDSFLA--ACPKLKIVFAALKGYDNFDVDACTKRGVWFGIVPDLL 100 Query: 104 PYSVAEFAVGLLLAVNRKIARASMRTREGNFE--LEGLMGFDLHGKTVGVIGTGKIGALF 161 AE VGLLL + R + R G F L G L G T+G+IG G++G Sbjct: 101 TVPTAELTVGLLLGLTRHVMAGDDHVRSGTFHGWRPKLYGAGLAGSTIGIIGMGRVGKAI 160 Query: 162 ARIMAGFGCTLLGSDMYQ-NPVFE-ALGGRYVDKQALFAASDVISLHCPLTEATRYIVNA 219 A+ ++GF + D NPV E A R V L SD + P+T T ++++A Sbjct: 161 AKRLSGFEMNAVYCDSVPLNPVDEQAWNARQVSFDELLTCSDFVVPMLPMTSDTFHLIDA 220 Query: 220 ESLAATKPGCILVNTSRGGLVDTEAVIAALKSGQLRGLAIDVYEQE 265 +++ + G L+NTSRG +VD AV+ AL G L G A DV+E E Sbjct: 221 HAISKMRRGSYLLNTSRGSVVDENAVVEALNQGHLAGYAADVFEME 266 >gi|152982822|ref|YP_001355375.1| D-isomer specific 2-hydroxyacid dehydrogenase [Janthinobacterium sp. Marseille] Length = 323 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 84/280 (30%), Positives = 134/280 (47%), Gaps = 32/280 (11%) Query: 58 LGAAVLERLAAQGVRFITTRSTGFNHIDTQAARRLGISVAR-VADYSPYSVAEFAVGLLL 116 L +LE+L ++ I HID AA GI++ V D P + AE L++ Sbjct: 59 LSRQLLEKLP--NLKLIAQTGKVSGHIDLPAAAARGITIVEGVGD--PTAPAELTWTLIM 114 Query: 117 AVNRKIARASMRTREGNFELEGL------MGFDLHGKTVGVIGTGKIGALFARIMAGFGC 170 A RK+ + ++G +++ + +G L G+T+ + G G+IG + A FG Sbjct: 115 AAMRKLPQYVNNLKDGLWQIASMSPARNTLGTALKGRTLAIWGYGRIGQMIAAYGKAFGM 174 Query: 171 TLLGSDMYQNPV-FEAL---GGRYVD-KQALFAASDVISLHCPLTEATRYIVNAESLAAT 225 ++ ++ P EA G R +QA F+ +DV++LH L +ATR IV A+ LA Sbjct: 175 DVM---VWGRPASCEAAVRDGYRAASSQQAFFSEADVLTLHLRLNDATRGIVTADDLARM 231 Query: 226 KPGCILVNTSRGGLVDTEAVIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQR 285 KP + VNTSR LV T+A+ AAL+ G+ A+DV+E E L Sbjct: 232 KPQALFVNTSRAELVATDALQAALEQGRPGYAALDVFENEP------LPPG-------SP 278 Query: 286 LVSFPNVIVTGHQAFFTEEAIGQIMQTTIANLSDFAAGVS 325 L+ NV+ + H + +++ + N+ DFA G Sbjct: 279 LLRMENVLASPHLGYVEKDSYELYFGSAFQNIVDFANGTP 318 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.322 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 707,470 Number of extensions: 28823 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 95 Number of HSP's successfully gapped: 4 Length of query: 332 Length of database: 1,221,021 Length adjustment: 84 Effective length of query: 248 Effective length of database: 910,473 Effective search space: 225797304 Effective search space used: 225797304 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)