BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2450.p (493 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979948|ref|YP_001354595.1| succinate-semialdehyde dehydrog... 250 1e-67 gi|152980306|ref|YP_001355332.1| methylmalonate-semialdehyde deh... 147 2e-36 gi|152980627|ref|YP_001353009.1| bifunctional proline dehydrogen... 122 4e-29 >gi|152979948|ref|YP_001354595.1| succinate-semialdehyde dehydrogenase (NADP+) [Janthinobacterium sp. Marseille] Length = 490 Score = 250 bits (639), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 163/470 (34%), Positives = 249/470 (52%), Gaps = 14/470 (2%) Query: 17 INGEWIPARSGNEFTSTNPASGELLAHCANADREDVDAAVQAAWSAYKTWKDTSPAERAG 76 ING W+ ++ F NPA G + N ++ + A+QAA +A+ W + ERAG Sbjct: 21 INGAWVTGKA--SFDVNNPADGSKITDVPNLGAQETEQAIQAAHAAFPAWAAKTGKERAG 78 Query: 77 FLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDI--PSGIDHFRYFAAAIRTDEGSAT 134 + D I A A A + T + GKP+ E+ I S I+ F A + D ++T Sbjct: 79 IMRIWFDLIIANADDLAALMTAEQGKPLIESRGEVIYGASFIEWFAEEAKRVSGDVMAST 138 Query: 135 RLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDTSLSLLEL 194 DK T+++ ++PIGV I PWNFP M K+APA+AAG +VIKP+ T LS L + Sbjct: 139 WSDKRTVTI--KQPIGVCASITPWNFPIAMITRKVAPAIAAGCTIVIKPAEQTPLSALAI 196 Query: 195 GKVL-KDILPKGVVNIVTGKGSTT---GNYMLEHEGFRKLAFTGSTDIGYTVAEAAAKKL 250 ++ + +P GV+N+VT + G + R ++FTGST +G + E A + Sbjct: 197 AELAHRAGIPPGVINVVTSDSDQSIAVGKVLCSSPIVRHVSFTGSTPVGRILMEQCAPTV 256 Query: 251 IPSTLELGGKSANIIFPDAPWEKMIEGVQLGILFNQGQVCCAGSRIFVHKSIYNRFVEEA 310 LELGG + I+F DA + + G N GQ C +R +VH+SIY+ FV++ Sbjct: 257 KKIGLELGGHAPFIVFDDADLDAAVVGALQSKYRNAGQTCVCTNRFYVHESIYDTFVQKL 316 Query: 311 GKAFSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEGEVLVGGHRIGRTELQDGN 370 G +++ V GPL++A +K V +V ++G ++ G G+ G Sbjct: 317 GAGAAKIKVGNGVEQGVLQGPLIDAQAVKKVEQHVSDAVSKGAKVITG---GKQHALGGY 373 Query: 371 FMEPTLIATTN-NSRVAREEIFGPVGAVIAFEDEDEVIAMANDSEFGLGGAVWTKTLNTA 429 F EPT+++ + + + EE FGPV AVI F+ EDE +A AND++FGL +++ + Sbjct: 374 FYEPTVLSNISPDMLIMHEETFGPVAAVIKFKTEDEAVAAANDTDFGLASYFYSRDIGRV 433 Query: 430 LRVASGVETGRMWVNTYNQLPAHAPFGGYKKSGIGRENHKMMLEHYSQCK 479 RVA +E G + +NT APFGG K+SG+GRE K +E Y + K Sbjct: 434 WRVAEKLEYGMVGINTGLISNEVAPFGGVKQSGLGREGSKYGIEEYLEIK 483 >gi|152980306|ref|YP_001355332.1| methylmalonate-semialdehyde dehydrogenase [Janthinobacterium sp. Marseille] Length = 505 Score = 147 bits (370), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 144/487 (29%), Positives = 225/487 (46%), Gaps = 22/487 (4%) Query: 12 QYKLLINGEWIPARSGNEFTSTNPASGELLAHCANADREDVDAAVQAAWSAYKTWKDTSP 71 + K ING G + NPA+GE A A DV A V A +A W DT+P Sbjct: 6 KIKHYINGRLTETAGGRQQDVFNPATGEASAQVVLASDADVQAVVATAKAAAPAWADTAP 65 Query: 72 AERAGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAAAIRTDEG 131 +RA L + D IE A T ++GK + ++ G++ + A + Sbjct: 66 LKRARILFKFKDLIEKHHDDLAAAITREHGKVFSDAKG-EVTRGLEVVEF---ACGVPQL 121 Query: 132 SATRLDKNTLSLI----LREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSS-D 186 T+ N I LR+P+GV I P+NFPF++ W ALA GN ++KPS D Sbjct: 122 LKTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPFMVPMWMAPVALATGNTFILKPSERD 181 Query: 187 TSLSLLELGKVLKDILPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIG-YTVAEA 245 S SL+ + + LP GV N++ G + +L+H + ++F GST I Y A Sbjct: 182 PSPSLMVAELLQQAGLPDGVFNVLQGDKLAV-DGLLQHPDVKAVSFVGSTPIAEYIYATG 240 Query: 246 AAKKLIPSTLEL----GGKSANIIFPDAPWEKMIEGVQLGILFNQGQVCCAGSRIFVHKS 301 AA+K L + G K+ ++ PDA E+ ++ + + G+ C A S Sbjct: 241 AARKGGSYPLRVQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGD 300 Query: 302 IYNRFVEEAGKAFSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEG-EVLVGGHR 360 I ++ V + V A+ +MGP+V+A + GY+D G G ++LV G Sbjct: 301 IADKLVAALIPRVKSLKVKNGMEADAEMGPVVSAAHKAKIEGYIDAGVKAGAKLLVDGRG 360 Query: 361 IGRTELQDGNFMEPTLI-ATTNNSRVAREEIFGPVGAVIAFEDEDEVIAMANDSEFGLGG 419 + G F+ L T + +EEIFGPV V+ + + + N EFG G Sbjct: 361 LKVAGHDKGFFLGGCLFDHVTPEMTIYQEEIFGPVLCVLRVPNFAAALELINRHEFGNGV 420 Query: 420 AVWTKTLNTALRVASGVETGRMWVNTYNQLP-AHAPFGGYKKSGIGRENH---KMMLEHY 475 +++T NTA + VE G + +N +P A FGG+K+S G + H + ++ Y Sbjct: 421 SLFTSDGNTAREFSRRVEVGMVGINVPIPVPMAWHSFGGWKRSMFG-DTHAYGEEGIKFY 479 Query: 476 SQCKNIF 482 ++ K+I Sbjct: 480 TRYKSIM 486 >gi|152980627|ref|YP_001353009.1| bifunctional proline dehydrogenase /delta-1-pyrroline-5-carboxylate dehydrogenase [Janthinobacterium sp. Marseille] Length = 1242 Score = 122 bits (307), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 116/463 (25%), Positives = 206/463 (44%), Gaps = 39/463 (8%) Query: 20 EWIPARSGNEFTST-----NPAS-GELLAHCANADREDVDAAVQAAWSAYKTWKDTSPAE 73 +W A S + +S NPA+ +++ AD ++ A+Q A S TW+ AE Sbjct: 585 QWRAAPSCTQVSSACIEVRNPANHADVVGTVQQADAAVLELALQHATSGINTWQAKPIAE 644 Query: 74 RAGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAA-AIRTDEGS 132 RA L + AD + A + + GK + + ++ ID RY++A AI +D Sbjct: 645 RAAILLQAADLFQQHATELIALLVREAGKTLPNAIG-ELREAIDFLRYYSAQAIASDAN- 702 Query: 133 ATRLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDTSLSLL 192 +P+G+V I PWNFP + +++ ALAA N V+ KP+ T L Sbjct: 703 --------------QPLGLVACISPWNFPLSIFTGQVSAALAAANVVLAKPAEQTPLIAC 748 Query: 193 ELGKVLKDI-LPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIGYTVAEAAAK--- 248 ++L +P+ + + G G T G ++ + FTGST + ++ + Sbjct: 749 RAVQLLHQAGVPENALLFLPGSGETIGAQLVTDPRVSAVVFTGSTQVAQSIQRKLVQRSI 808 Query: 249 ---KLIPSTLELGGKSANIIFPDAPWEKMIEGVQLGILFNQGQVCCAGSRIFVHKSIYNR 305 K IP E GG++ I+ A E+++ V + GQ C A + + I + Sbjct: 809 SEGKDIPLIAETGGQNTMIVDSSALCEQVVRDVISSAFDSAGQRCSALRVLCLQDDIAEQ 868 Query: 306 FVEEAGKAFSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEGEVLVGGHRIGRTE 365 ++ A ++ + P T +GP+++A + Y+ + G+ + R Sbjct: 869 TLQMLHGAMQELRIGRPDHLATDVGPVIDAEAQAHLQAYIRQAQAAGKQV----RQLPLP 924 Query: 366 LQDGNFMEPTLIATTNNSRVAREEIFGPVGAVIAFEDED--EVIAMANDSEFGLGGAVWT 423 + +G+F+ P +I + + + R E+FGPV V+ + +D +++ N + FGL + T Sbjct: 925 VTNGSFVAPCIIEIADINELQR-EVFGPVLHVLRYRRDDLPQLLMDINSTGFGLTLGIHT 983 Query: 424 KTLNTALRVASGVETGRMWV--NTYNQLPAHAPFGGYKKSGIG 464 + T V + G ++V N + PFGG SG G Sbjct: 984 RIDETIDYVVRHAQVGNIYVNRNIVGAVVGVQPFGGEGLSGTG 1026 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,177,634 Number of extensions: 51049 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 3 Length of query: 493 Length of database: 1,221,021 Length adjustment: 87 Effective length of query: 406 Effective length of database: 899,382 Effective search space: 365149092 Effective search space used: 365149092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)