BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2225.p (3675 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janth... 82 8e-16 gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthino... 82 9e-16 gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligas... 71 1e-12 >gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 634 Score = 81.6 bits (200), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 131/576 (22%), Positives = 230/576 (39%), Gaps = 97/576 (16%) Query: 1994 GPTIRADDVIERWAAQVSQQPGAAALTDGNHALDM---AGLCAWVEQMAQQMRQRGIGCE 2050 G T + ++R A + QP A++ A L A V + A M+ G+G Sbjct: 54 GETNLCYNAVDRHLATRADQPALVAISTETDTETTYTYAQLHAEVSRSAAMMQALGVGQG 113 Query: 2051 DRVAIHAERSCAFVVAVLATLRVGAAYVPLDPALPAERLHYQLQDSAAVLLLSATAPDWA 2110 DRV I+ A+LA R+GA + + + L ++ D+ VL++SA A Sbjct: 114 DRVLIYMPMVAEAAFAMLACARIGAIHSVVFGGFASTSLATRIDDATPVLIISADAGSRG 173 Query: 2111 ASVP--------------------------VLPLASLHG------------VPKRVPYCW 2132 V + P+ ++ G + VP W Sbjct: 174 CHVTPYKCLLDEAIALAEHKPAHVLMLDRGLAPMKTIEGRDVDYATLRRQHMDAVVPVAW 233 Query: 2133 PAVHPGQAAYVIYTSGSTGRPKGVVVSRGALANYVAGVLERLDLREQDRSMAMVSTVGAD 2192 + +Y++YTSG+TG+PKGV+ G A +A ++ + + + S +G Sbjct: 234 --LESNAPSYILYTSGTTGKPKGVLRDVGGYAVALATSMDLIFCGKPGETFFCTSDIGWV 291 Query: 2193 LGHT-VLFGALCSGRLLHLIPPQCSFDPDAMA--SYMEQHQVDVLKIVPSHLQALLHAAS 2249 +GH+ +++G L +G + ++ PDA S +E+++V + P+ ++ L Sbjct: 292 VGHSYIIYGPLLAG-MTTILYEGLPIRPDASIWWSIVEKYKVTRMSSAPTAIRVL--KKQ 348 Query: 2250 PASVLPR------SRLVLGGEASRKPLLAHIASLRPQCRVMNHYGPTETTVGVLTQEASS 2303 P +L + L L GE + I+ + V+++Y TET +++ Sbjct: 349 PLELLQKHDLSSLKALYLAGEPLDQTTSDWISGVL-GVPVIDNYWQTETGWPIISVAKGV 407 Query: 2304 ALDEADSLPIGQPIAQARAYVLDAEL---------------QPVPQGVAGELYLAGHGVA 2348 S GQP+ +A V++ E P+P G LY Sbjct: 408 GDKPTRSGSPGQPMYGYKAKVINEETGEECAPNEKGVVIIEGPLPPGCLQTLY------- 460 Query: 2349 RAYQQRPAQSAERFVADLLEPGQR-MYRSGDRVRQLADGSLEFLGRLDGQIKVRGYRVEL 2407 +RFV+ +R Y + D + ADG LGR D I V G+R+ Sbjct: 461 --------GDDQRFVSTYWRNFKREAYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGT 512 Query: 2408 REIAALLQSLEAVAQAEVVVHSEPAQPGPDGEEVAPQII-----AYLVPMGGQTLDVADL 2462 REI + S V++ VV P + G+ +I A P + L+ A++ Sbjct: 513 REIEESISSHANVSEVAVVGVENPIK----GQCAVAFVILKNAAAAADPDEARKLE-AEI 567 Query: 2463 RQQLATRLPDYMMPTALVPLHVMPLTPNGKLDRQAL 2498 + + RL P+ + + +P T +GKL R+++ Sbjct: 568 MKVVDKRLGPIGRPSHVYFVSALPKTRSGKLLRRSI 603 >gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 664 Score = 81.6 bits (200), Expect = 9e-16, Method: Composition-based stats. Identities = 133/531 (25%), Positives = 217/531 (40%), Gaps = 69/531 (12%) Query: 3092 MSYGQLNTRVNQLAHYLLQRGLQPEARVGVALERSADMVVAVLAILKAGATYVPLDPDYP 3151 ++Y +L RV + A+ L + G+Q RV + + S + V A+ A + GAT+ + + Sbjct: 118 VTYKELYERVCKFANGLKELGIQKGDRVIIYMPMSIEGVAAMQACARIGATHSVVFGGFS 177 Query: 3152 VQRLAA-IIEDSALSLLLSQTSV--SQRLP--------------------------GGR- 3181 + L II+ A+++L S V + LP GG Sbjct: 178 AKSLQERIIDAGAVAVLTSDYQVRGGKNLPLKSIVDEALALGGCDTIRNVVVYKRTGGEM 237 Query: 3182 ---AEQTLYLDQLDLQAQPHDEPDVTIHSDSLAYVIYTSGSTGRPKGVGISHRCLMEHGQ 3238 A + ++L L + + EP V + ++ +++YTSGSTG+PKGV S + Sbjct: 238 NFVAGRDIWLHDLVDKQKAECEP-VPVGAEHPLFILYTSGSTGKPKGVQHSSGGYLLWAM 296 Query: 3239 IAADF-FGLTPQDCMLQFSTLNF-DGCIEQLFPPLLVGAA-VVLRG-PELWDSQRLHVEL 3294 + + F + P D + + + G + PL GA +V G P ++ R + Sbjct: 297 LTMKWSFDIKPNDVYWCTADIGWITGHTYVTYGPLACGATQIVFEGVPMFPNAGRFWDMV 356 Query: 3295 LAHRISVL-DLTTAYWLLLVQDFARMGVP----DYGVLRRVHVGGEAMPPEG-IKAWRDA 3348 H++++ TA L+ A V D LR + GE + PE + +++ Sbjct: 357 QKHKVTIFYTAPTAIRSLIKAADADPAVHPSKYDLSSLRLLGSVGEPINPEAWMWYYKNI 416 Query: 3349 GLAQVELFNTYGPTE--ATVTASILSCAPYVSGQTVLPAQMPIGAPLAGRKLRVLDAELS 3406 G + + +T+ TE V + + P V G LP L G V+D Sbjct: 417 GGERCPIADTFWQTETGGHVISPMPGATPMVPGSCTLP--------LPGIAAAVVDEVGH 468 Query: 3407 PLPIGVAGELCIGGEL--LARGYLNQPGLSAERFVADPLGDGGARLYRTGD-LVRWTHAG 3463 L G G L I + R N P + + LG +Y GD +R G Sbjct: 469 DLANGKGGLLVIKRPWPSMIRNIWNDPERFQKSYFPHELG---GTIYLAGDGAIRNEETG 525 Query: 3464 QLEYLGRIDHQVKIRGFRIEMGEIESIMLAQPEVREAVVVAR--EGVGSLQLVAYVTPQH 3521 GRID + I G R+ EIES ++A P V EA VV + E G + A+V + Sbjct: 526 YFTITGRIDDVLNISGHRMGTMEIESALVANPIVAEAAVVGKPDETTGE-SICAFVVLKR 584 Query: 3522 GRHIE------TAELRECLVRHLPDYMVPAALVVLPALPLNANGKVDRKAL 3566 R ELR+ + + + P + LP +GK+ R+ L Sbjct: 585 SRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLL 635 Score = 75.9 bits (185), Expect = 4e-14, Method: Composition-based stats. Identities = 130/536 (24%), Positives = 210/536 (39%), Gaps = 89/536 (16%) Query: 538 QLSYGALERRANQLAHRLIRLGAGPEVKVGIAVERSLEMVIGLLGILKAGAAYVPLDPEY 597 +++Y L R + A+ L LG +V I + S+E V + + GA + + + Sbjct: 117 RVTYKELYERVCKFANGLKELGIQKGDRVIIYMPMSIEGVAAMQACARIGATHSVVFGGF 176 Query: 598 PAQRLQYMMQDSGMRLLLTQQHVL---PTLPP---------------------------- 626 A+ LQ + D+G +LT + + LP Sbjct: 177 SAKSLQERIIDAGAVAVLTSDYQVRGGKNLPLKSIVDEALALGGCDTIRNVVVYKRTGGE 236 Query: 627 ---LAGLPVLALDQLDLDDEAHQPPAVRVHGENLAYVIYTSGSTGQPKGVANRHCALRNR 683 +AG + D +D +P V V E+ +++YTSGSTG+PKGV + Sbjct: 237 MNFVAGRDIWLHDLVDKQKAECEP--VPVGAEHPLFILYTSGSTGKPKGVQHSSGGY--- 291 Query: 684 LAW----MQQAYRLEPHDTVLQKTPFSFDVS-VWEFFWPLMIGA-RLALAQPGDHRDPAR 737 L W M+ ++ ++P+D + + + PL GA ++ + R Sbjct: 292 LLWAMLTMKWSFDIKPNDVYWCTADIGWITGHTYVTYGPLACGATQIVFEGVPMFPNAGR 351 Query: 738 LVEQIQRHAVSTLHFVPSMLQAFL--------AHDEIEGCSSLHRLICSGEALPVEAQQG 789 + +Q+H V+ + P+ +++ + H SSL L GE + EA Sbjct: 352 FWDMVQKHKVTIFYTAPTAIRSLIKAADADPAVHPSKYDLSSLRLLGSVGEPINPEAWMW 411 Query: 790 VFARLPQVALYNLYGPTEAAIDVTHWTCRADGR--SQVPIGRP-ISSIRTLVLDGDLNLA 846 + + G I T W G S +P P + TL L G Sbjct: 412 YYKNI---------GGERCPIADTFWQTETGGHVISPMPGATPMVPGSCTLPLPGIAAAV 462 Query: 847 PQGVPGELYLGGIGLARGYLGRAGLS--------AERFVADPFSAD-GERLYRTGD-LVR 896 V +L G GL + R S ERF F + G +Y GD +R Sbjct: 463 VDEVGHDLANGKGGLL--VIKRPWPSMIRNIWNDPERFQKSYFPHELGGTIYLAGDGAIR 520 Query: 897 WNREGQLDYLGRLDHQVKIRGLRIELGEIEARLLAQPGVREAVVVAR--ESAGTSRLVAY 954 G GR+D + I G R+ EIE+ L+A P V EA VV + E+ G S + A+ Sbjct: 521 NEETGYFTITGRIDDVLNISGHRMGTMEIESALVANPIVAEAAVVGKPDETTGES-ICAF 579 Query: 955 V-------SGTDL--VAATLRSALTKDLPDYMVPALIEVLEHLPLNANGKVDRRAL 1001 V +G + +A LR + K++ P I ++LP +GK+ RR L Sbjct: 580 VVLKRSRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLL 635 Score = 70.9 bits (172), Expect = 1e-12, Method: Composition-based stats. Identities = 130/545 (23%), Positives = 210/545 (38%), Gaps = 117/545 (21%) Query: 2035 VEQMAQQMRQRGIGCEDRVAIHAERSCAFVVAVLATLRVGAAYVPLDPALPAERLHYQLQ 2094 V + A +++ GI DRV I+ S V A+ A R+GA + + A+ L ++ Sbjct: 127 VCKFANGLKELGIQKGDRVIIYMPMSIEGVAAMQACARIGATHSVVFGGFSAKSLQERII 186 Query: 2095 DSAAVLLLSATAPDWAASVPVLPLAS---------------------------------- 2120 D+ AV +L T+ LPL S Sbjct: 187 DAGAVAVL--TSDYQVRGGKNLPLKSIVDEALALGGCDTIRNVVVYKRTGGEMNFVAGRD 244 Query: 2121 --LHG-VPKRVPYCWP----AVHPGQAAYVIYTSGSTGRPKGVVVSRGALANYVAGVLE- 2172 LH V K+ C P A HP +++YTSGSTG+PKGV S G + ++ Sbjct: 245 IWLHDLVDKQKAECEPVPVGAEHP---LFILYTSGSTGKPKGVQHSSGGYLLWAMLTMKW 301 Query: 2173 RLDLREQDRSMAMVSTVGADLGHT-VLFGALCSGRLLHLIPPQCSFDPDA--MASYMEQH 2229 D++ D + +G GHT V +G L G ++ P+A +++H Sbjct: 302 SFDIKPNDVYWC-TADIGWITGHTYVTYGPLACGA-TQIVFEGVPMFPNAGRFWDMVQKH 359 Query: 2230 QVDVLKIVPSHLQALLHAASPASVLPRSRLVLGGEASRKPLLAHIAS-LRPQ-------- 2280 +V + P+ +++L+ AA + S+ L S LL + + P+ Sbjct: 360 KVTIFYTAPTAIRSLIKAADADPAVHPSKYDL----SSLRLLGSVGEPINPEAWMWYYKN 415 Query: 2281 -----CRVMNHYGPTETTVGVLTQEASSALDEADSLPIGQPIAQARAYVLDAELQPVPQG 2335 C + + + TET V++ P+ A V + P+P Sbjct: 416 IGGERCPIADTFWQTETGGHVIS-----------------PMPGATPMVPGSCTLPLPGI 458 Query: 2336 VAGELYLAGHGVARAYQ-----QRPAQS--------AERFVADLL--EPGQRMYRSGD-R 2379 A + GH +A +RP S ERF E G +Y +GD Sbjct: 459 AAAVVDEVGHDLANGKGGLLVIKRPWPSMIRNIWNDPERFQKSYFPHELGGTIYLAGDGA 518 Query: 2380 VRQLADGSLEFLGRLDGQIKVRGYRVELREIAALLQSLEAVAQAEVVVHSEPAQPGPDGE 2439 +R G GR+D + + G+R+ EI + L + VA+A VV G E Sbjct: 519 IRNEETGYFTITGRIDDVLNISGHRMGTMEIESALVANPIVAEAAVV--------GKPDE 570 Query: 2440 EVAPQIIAYLV-----PMGGQTLDVA-DLRQQLATRLPDYMMPTALVPLHVMPLTPNGKL 2493 I A++V P G + +A +LR +A + P + +P T +GK+ Sbjct: 571 TTGESICAFVVLKRSRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKI 630 Query: 2494 DRQAL 2498 R+ L Sbjct: 631 MRRLL 635 >gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligase [Janthinobacterium sp. Marseille] Length = 560 Score = 71.2 bits (173), Expect = 1e-12, Method: Composition-based stats. Identities = 69/277 (24%), Positives = 119/277 (42%), Gaps = 23/277 (8%) Query: 3293 ELLAHRISVLDLTTAYW--LLLVQDFARMGVPDYGVLRRVHVGGEAMPPEGIKAWRD-AG 3349 EL+ +++ L + LL DFA++ D+ L+ + GG A+ WR+ G Sbjct: 297 ELMKYKVHFLPAVNTLYNGLLNNPDFAKV---DFSELKLCNGGGMAVQKVVNDKWREVTG 353 Query: 3350 LAQVELFNTYGPTEATVTASILSCAPYVSGQTVLPAQMPIGAPLAGRKLRVLDAELSPLP 3409 A E YG +E + TA+ + T IG P++ +L+++D++ + +P Sbjct: 354 CAICE---GYGLSETSPTAT-------ANPPTTNEFTGTIGLPVSSTELKIIDSDDNEVP 403 Query: 3410 IGVAGELCIGGELLARGYLNQPGLSAERFVADPLGDGGARLYRTGDLVRWTHAGQLEYLG 3469 G AGE+ I G + GY N P +A+ AD ++TGD+ G + + Sbjct: 404 HGSAGEIAIRGPQVMAGYWNNPVETAQSMTADG-------FFKTGDIGIMDERGFTKIVD 456 Query: 3470 RIDHQVKIRGFRIEMGEIESIMLAQPEVREAVVVAREGVGSLQLVAYVTPQHGRHIETAE 3529 R + + GF + EIE ++ A P V E + + + V + + AE Sbjct: 457 RKKDMILVSGFNVYPSEIEGVVAAHPGVLECACIGVPDEHTGEAVKLFVVRRDPALTEAE 516 Query: 3530 LRECLVRHLPDYMVPAALVVLPALPLNANGKVDRKAL 3566 L Y P ++ LP + GK+ R+ L Sbjct: 517 LMAYCREQFTGYKKPKSIEFRTELPKSNVGKILRREL 553 Score = 68.6 bits (166), Expect = 7e-12, Method: Composition-based stats. Identities = 67/279 (24%), Positives = 112/279 (40%), Gaps = 12/279 (4%) Query: 733 RDPARLVEQIQRHAVSTLHFVPSMLQAFLAHDEIEGCSSLHRLICSGEALPVE-AQQGVF 791 RD ++++ ++ V L V ++ L + + +C+G + V+ + Sbjct: 289 RDIPGFIKELMKYKVHFLPAVNTLYNGLLNNPDFAKVDFSELKLCNGGGMAVQKVVNDKW 348 Query: 792 ARLPQVALYNLYGPTEAAIDVTHWTCRADGRSQVPIGRPISSIRTLVLDGDLNLAPQGVP 851 + A+ YG +E + T + + IG P+SS ++D D N P G Sbjct: 349 REVTGCAICEGYGLSETSPTATANPPTTNEFTGT-IGLPVSSTELKIIDSDDNEVPHGSA 407 Query: 852 GELYLGGIGLARGYLGRAGLSAERFVADPFSADGERLYRTGDLVRWNREGQLDYLGRLDH 911 GE+ + G + GY +A+ AD F ++TGD+ + G + R Sbjct: 408 GEIAIRGPQVMAGYWNNPVETAQSMTADGF-------FKTGDIGIMDERGFTKIVDRKKD 460 Query: 912 QVKIRGLRIELGEIEARLLAQPGVREAVVVARESAGTSRLVA-YVSGTD--LVAATLRSA 968 + + G + EIE + A PGV E + T V +V D L A L + Sbjct: 461 MILVSGFNVYPSEIEGVVAAHPGVLECACIGVPDEHTGEAVKLFVVRRDPALTEAELMAY 520 Query: 969 LTKDLPDYMVPALIEVLEHLPLNANGKVDRRALPDPAQP 1007 + Y P IE LP + GK+ RR L D +P Sbjct: 521 CREQFTGYKKPKSIEFRTELPKSNVGKILRRELRDEKKP 559 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 8,624,875 Number of extensions: 369930 Number of successful extensions: 1080 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 1070 Number of HSP's successfully gapped: 7 Length of query: 3675 Length of database: 1,221,021 Length adjustment: 102 Effective length of query: 3573 Effective length of database: 843,927 Effective search space: 3015351171 Effective search space used: 3015351171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 139 (58.2 bits)