BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2223.p (1789 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janth... 69 2e-12 gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligas... 65 3e-11 >gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 634 Score = 69.3 bits (168), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 132/564 (23%), Positives = 223/564 (39%), Gaps = 116/564 (20%) Query: 55 LAQRVRALAAVLQAR-LGRGERALILLDNDEYYAISMFACFYAGVIAVPVFPPESASAAH 113 L V AA++QA +G+G+R LI + A +M AC G I VF ++++ Sbjct: 94 LHAEVSRSAAMMQALGVGQGDRVLIYMPMVAEAAFAMLACARIGAIHSVVFGGFASTSLA 153 Query: 114 TGRLAG------IAADAGARG-----------------------LLILDANRALVAACEG 144 T R+ I+ADAG+RG +L+LD A + EG Sbjct: 154 T-RIDDATPVLIISADAGSRGCHVTPYKCLLDEAIALAEHKPAHVLMLDRGLAPMKTIEG 212 Query: 145 -------LQQAEMIAVDQIGAGDRGPW-QAAAPDPQEIAFLQYTSGSTSAPKGVMVTHAA 196 L++ M AV + W ++ AP +++ YTSG+T PKGV+ Sbjct: 213 RDVDYATLRRQHMDAVVPVA------WLESNAP-----SYILYTSGTTGKPKGVLRDVGG 261 Query: 197 LMANEAAIREGLSIGE-GDSFCVWSPLFHDMGLIGG----LLQPFYSGIVCVLCSPRYFL 251 A + + G+ G++F S D+G + G + P +G+ +L + Sbjct: 262 YAVALATSMDLIFCGKPGETFFCTS----DIGWVVGHSYIIYGPLLAGMTTIL-----YE 312 Query: 252 ERPVR-----WLQMIARHRVSISGGPDFAYRLCLDRIKDRQLEGL---DLSCWRVAYTGA 303 P+R W ++ +++V+ A R+ +K + LE L DLS + Y Sbjct: 313 GLPIRPDASIWWSIVEKYKVTRMSSAPTAIRV----LKKQPLELLQKHDLSSLKALYLAG 368 Query: 304 EPVRHQTMEAFVQRFASVGLDPAAVYPCYGLAEATLFVTGGRRGAGLRVQRYLPHALRSR 363 EP+ QT ++ G+ V Y E + +G G + Sbjct: 369 EPL-DQTTSDWIS-----GVLGVPVIDNYWQTETGWPIISVAKGVGDK------------ 410 Query: 364 LALPADAEEPAVALVACGRAPSQHQVRIAADASGAPAAPGGIGEIWVTGPSIARGYWKQA 423 P + P G+ ++ ++ + +G AP G + + GP + G + Sbjct: 411 ---PTRSGSP-------GQPMYGYKAKVINEETGEECAPNEKGVVIIEGP-LPPGCLQTL 459 Query: 424 LQSAQTFVELENQTWLRTGDLGF-W----ADGELFVAGRLKDMIIVRGHNIYPQDLEQAL 478 Q FV + + R F W ADG F+ GR D+I V GH + +++E+++ Sbjct: 460 YGDDQRFVSTYWRNFKREAYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESI 519 Query: 479 EAEVEALRKGRIAVFAVSLDTEEGVGVAVEVSRGMQKLVAP---QALCEAVATVVQRQCG 535 + +AV V + VA + + P + L + VV ++ G Sbjct: 520 SSHANV---SEVAVVGVENPIKGQCAVAFVILKNAAAAADPDEARKLEAEIMKVVDKRLG 576 Query: 536 EAPQVLVLLNPGGLPKTSSGKLQR 559 + + LPKT SGKL R Sbjct: 577 PIGRPSHVYFVSALPKTRSGKLLR 600 >gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligase [Janthinobacterium sp. Marseille] Length = 560 Score = 65.5 bits (158), Expect = 3e-11, Method: Composition-based stats. Identities = 133/582 (22%), Positives = 220/582 (37%), Gaps = 128/582 (21%) Query: 42 AGVDQERRFSYQVLAQRVRALAAVLQA-RLGRGERALILLDNDEYYAISMFACFYAGVIA 100 A V ++ +Y+ + + A LQ+ L +G R +++ N Y I++ A AG + Sbjct: 41 ACVCMDKALTYREVDHLSTEVGAWLQSIGLQKGARVALMMPNVLQYPIAIAAVLRAGYVI 100 Query: 101 V---PVFPPESASAAHTGRLAGIAADAGARGLLILDANRALVAACEGLQQAEMIAVDQIG 157 V P++ P T D+GA ++IL+ A + Q + + V +G Sbjct: 101 VNVNPLYKPRELEHQLT--------DSGAEAIIILENFAATLEKVIASTQVKHVIVATMG 152 Query: 158 ---AGDRG-----------------------PW------------QAAAPDPQEIAFLQY 179 G +G P+ Q A Q++AFLQY Sbjct: 153 DLFGGLKGGIVNFVVRHVKKMVPAYSLPQAVPFKEVLRQAAGMTLQRPALGHQDLAFLQY 212 Query: 180 TSGSTSAPKGVMVTH----AALMANEAAIREGLSIGEGDS---FCVWSPLFHDMGLIG-G 231 T G+T KG M+TH A ++ NEA I L+ D F PL+H L G Sbjct: 213 TGGTTGTAKGAMLTHRNIIANVLQNEAWISPVLNTLPKDKQLIFVCALPLYHIFALTTCG 272 Query: 232 LLQPFYSGIVCVLCSPR-------YFLERPVRWLQMIARHRVSISGGPDFAYRLCLDRIK 284 +L + ++ +PR ++ V +L + + PDFA Sbjct: 273 MLGTHLGVMNLLIPNPRDIPGFIKELMKYKVHFLPAVNTLYNGLLNNPDFA--------- 323 Query: 285 DRQLEGLDLSCWRVAYTGAEPVRHQTMEAFVQRFASVGLDPAAVYPCYGLAEATLFVTGG 344 +D S ++ G V+ + + + + A+ YGL+E + T Sbjct: 324 -----KVDFSELKLCNGGGMAVQKVVNDKWRE------VTGCAICEGYGLSETSPTATAN 372 Query: 345 RRGAGLRVQRYLPHALRSRLALPADAEEPAVALVACGRAPSQHQVRIAADASGAPAAPGG 404 + + LP + E + I +D + P G Sbjct: 373 PPTT---------NEFTGTIGLPVSSTELKI---------------IDSDDNEVPH--GS 406 Query: 405 IGEIWVTGPSIARGYWKQALQSAQTFVELENQTWLRTGDLGFWAD-GELFVAGRLKDMII 463 GEI + GP + GYW +++AQ+ + + +TGD+G + G + R KDMI+ Sbjct: 407 AGEIAIRGPQVMAGYWNNPVETAQS---MTADGFFKTGDIGIMDERGFTKIVDRKKDMIL 463 Query: 464 VRGHNIYPQDLEQALEAEVEALRKGRIAVFAVSLDTEEGVGVAVEVSRGMQKLVAPQ--A 521 V G N+YP ++E + A L I V D G V + V R L + A Sbjct: 464 VSGFNVYPSEIEGVVAAHPGVLECACIGV----PDEHTGEAVKLFVVRRDPALTEAELMA 519 Query: 522 LCEAVATVVQRQCGEAPQVLVLLNPGGLPKTSSGKLQRGACR 563 C T ++ P+ + LPK++ GK+ R R Sbjct: 520 YCREQFTGYKK-----PKSIEFRTE--LPKSNVGKILRRELR 554 Score = 58.2 bits (139), Expect = 5e-09, Method: Composition-based stats. Identities = 58/244 (23%), Positives = 96/244 (39%), Gaps = 20/244 (8%) Query: 1428 DYGRLREIHIGGEAMPAESIATWRQAGLSAVRLLNTYGPTEAVVVASLLDCDPFVQGQRE 1487 D+ L+ + GG A+ WR+ ++ + YG +E A+ +P + Sbjct: 326 DFSELKLCNGGGMAVQKVVNDKWRE--VTGCAICEGYGLSETSPTAT---ANPPTTNE-- 378 Query: 1488 VPTQMPIGQPLAGRSLHVLDERLQALPAGVPGELYIGGDLLARGYLERPGLTAERFVADP 1547 IG P++ L ++D +P G GE+ I G + GY P TA+ AD Sbjct: 379 --FTGTIGLPVSSTELKIIDSDDNEVPHGSAGEIAIRGPQVMAGYWNNPVETAQSMTADG 436 Query: 1548 GSEAGGRLYRTGDLVCWNAQSELHYLGRSDLQVKLRGFRVELGEIEAQLLAQPEVRQAIV 1607 ++TGD+ + + + R + + GF V EIE + A P V + Sbjct: 437 -------FFKTGDIGIMDERGFTKIVDRKKDMILVSGFNVYPSEIEGVVAAHPGVLECAC 489 Query: 1608 VA----HQARLVGYASLYPGQELSTAQLRERLRASLPDYMVPATVIVLEQLPTNPNGKID 1663 + H V + L+ A+L R Y P ++ +LP + GKI Sbjct: 490 IGVPDEHTGEAVKLFVVRRDPALTEAELMAYCREQFTGYKKPKSIEFRTELPKSNVGKIL 549 Query: 1664 RHAL 1667 R L Sbjct: 550 RREL 553 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 4,160,756 Number of extensions: 178171 Number of successful extensions: 573 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 576 Number of HSP's successfully gapped: 4 Length of query: 1789 Length of database: 1,221,021 Length adjustment: 97 Effective length of query: 1692 Effective length of database: 862,412 Effective search space: 1459201104 Effective search space used: 1459201104 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 137 (57.4 bits)