BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2184.p (381 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981890|ref|YP_001351977.1| cation efflux system protein [J... 160 1e-40 gi|152981912|ref|YP_001352100.1| cation efflux protein [Janthino... 149 3e-37 gi|152983106|ref|YP_001353461.1| cobalt-zinc-cadmium resistance ... 108 7e-25 gi|152983091|ref|YP_001353421.1| cobalt-zinc-cadmium resistance ... 99 5e-22 gi|152981853|ref|YP_001353483.1| CzcB family cobalt/zinc/cadmium... 98 9e-22 >gi|152981890|ref|YP_001351977.1| cation efflux system protein [Janthinobacterium sp. Marseille] Length = 389 Score = 160 bits (405), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 106/320 (33%), Positives = 174/320 (54%), Gaps = 6/320 (1%) Query: 67 ALIEQRSIA---IPFS--LPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFE 121 A+IE ++I +P L A + D A+I +GRIV+++ GD VKAG VL E Sbjct: 60 AMIEAQNIPASPVPLGDQLSAHLVYDEDVTARIGVSISGRIVALKAAPGDVVKAGQVLAE 119 Query: 122 IESSDLAQAVSDERKAQSTLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAAR 181 I+S D+ A +D KA++ L RR ER + L + A +D+E AQ+DY QA +E AR Sbjct: 120 IDSPDVGTAYADINKAKADLDHKRRVYERAKDLGEGEAIAAKDVENAQADYVQAQAETAR 179 Query: 182 AHARVAALGGTAASGGSARLAVRAPVSGHVIELNAAVGSFWNDNTAS-VMTVADLSSVYV 240 A R+ L R+++ +P++G V E A + +S + V + +++ Sbjct: 180 AELRLKNLNPNGMVIRGQRISLLSPMNGVVTERTATPALEVSPGMSSPLFVVTNPKQLWL 239 Query: 241 TASAQEKDLARLHIGQDAEIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDER 300 EK L+++ +G I DAYP + AK+ +GQ +DP++R + +R + N D + Sbjct: 240 MIDVPEKMLSQIKLGSTVAIESDAYPNEKFTAKIVQLGQAVDPNSRRITIRARLNNPDGK 299 Query: 301 LKPGMFAQATLREQAHAGLLVPMTAVVQSGFANRVFVEVAPWKFEARKIMLGAQVGNDVE 360 L P MF +A +++ + G+ VP A+V G + VFV+ AP +F+ RK+ L + + Sbjct: 300 LLPEMFVRALVQQDSGTGVAVPNGALVTRGIYSFVFVQTAPGEFQQRKVKLLTRGSSVSY 359 Query: 361 VTDGLQAGTRVVVKDGVLLN 380 V +GL+ G +V K +LL+ Sbjct: 360 VGEGLKGGESIVTKGALLLD 379 >gi|152981912|ref|YP_001352100.1| cation efflux protein [Janthinobacterium sp. Marseille] Length = 383 Score = 149 bits (376), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 111/339 (32%), Positives = 172/339 (50%), Gaps = 10/339 (2%) Query: 51 TIVIPPASALRDSLKTALIEQRSIAIPFSLPASVEADPARLAKILPPTTGRIVSIQKRLG 110 +VI P ALR+ +K + + +P ++ D R+A+I P TGRI I LG Sbjct: 39 NVVIAP-EALRERVKIGTVGVAPFSDVLRVPGLIDFDRQRMARIGAPVTGRITDISANLG 97 Query: 111 DAVKAGDVLFEIESSDLAQAVSDERKAQSTLALTRRNLERQRSLDQANISARRDLEQAQS 170 +VK GDVL + S++L A KA++ L + N R R L A++ + +L++ ++ Sbjct: 98 QSVKPGDVLARLHSTELGSAQLAYLKARAQAELQQNNQTRARQLFAADVIGKAELQRREN 157 Query: 171 DYEQADSEAARAHARVAALGGTAAS----GGSARLAVRAPVS----GHVIELNAAVGSFW 222 + A +E+ A ++ LG ++A+ S ++ APV+ G V+EL + G Sbjct: 158 ESAVAAAESRAAAEQLKVLGMSSAAIARMAQSGEISSYAPVASTLHGSVVELKVSPGQVV 217 Query: 223 NDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDAEIALDAYPGQPLQAKVKSVGQVLD 282 A + TVADLS V+ A E+ + +GQ +I + A L K+ VGQ++D Sbjct: 218 QPADA-LFTVADLSHVWAVAEVPEQQADMVAVGQTVDIEVPALGNLKLSGKLIYVGQIVD 276 Query: 283 PDTRTVKVRILVTNTDERLKPGMFAQATLREQAHAGLLVPMTAVVQSGFANRVFVEVAPW 342 P TRTV VR + N D RLKP M A + + L++P AVV+ + V+VE P Sbjct: 277 PATRTVHVRTAIDNKDGRLKPAMLATMLIASRPTEQLVIPAAAVVRFENVDLVYVETQPG 336 Query: 343 KFEARKIMLGAQVGNDVEVTDGLQAGTRVVVKDGVLLND 381 F A K+ LG + V GL+AG R+ V LN+ Sbjct: 337 HFRATKVKLGQSNNGTLVVASGLKAGDRIAVDGAFHLNN 375 >gi|152983106|ref|YP_001353461.1| cobalt-zinc-cadmium resistance protein CzcB [Janthinobacterium sp. Marseille] Length = 526 Score = 108 bits (269), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 85/300 (28%), Positives = 148/300 (49%), Gaps = 12/300 (4%) Query: 74 IAIPFSLPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSD 133 I L + + R +++P G + S++ GD V+ G +L I S QA+SD Sbjct: 210 IKTELQLIGEIRLNEDRTVQVVPRLAGIVESVRANAGDRVRKGQILAVISS----QALSD 265 Query: 134 ERK----AQSTLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAARAHARVAAL 189 +R AQ LAL R ER+++L + ISA +D QA++ ++A A A ++AAL Sbjct: 266 QRSELLAAQKRLALARTTYEREKTLWEEKISAEQDYLQARTTMQEAQIIAQSAQQKLAAL 325 Query: 190 GGTAASGGSARLAVRAPVSGHVIELNAAVGSFWNDNTASVMTVADLSSVYVTASAQEKDL 249 G + +S + +R+P+ G + E + G+ ++ A++ ++DLS+V+V + + +D+ Sbjct: 326 GASPSSTNLTQYEIRSPIDGVITEKRISAGTVAKED-ANIFDISDLSTVWVDLTVRAQDI 384 Query: 250 ARLHIGQDAEIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQA 309 + GQ A + A+ + V +G ++ TR K RI++ N +PG+ Sbjct: 385 NAVKTGQKATVKATAFDAEGT-GTVSYIGSLVGEQTRAAKARIVMANPKGTWRPGLAVNV 443 Query: 310 TL-REQAHAGLLVPMTAVVQSGFANRVFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAG 368 L ++ + V ++ G A VF FEAR + LG + VEV GL+ G Sbjct: 444 ALVSDEVDVPVAVTTDSIQTLGNATVVFGRYGEL-FEARPLELGRRGAKYVEVLKGLETG 502 >gi|152983091|ref|YP_001353421.1| cobalt-zinc-cadmium resistance protein CzcB [Janthinobacterium sp. Marseille] Length = 396 Score = 99.0 bits (245), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 95/345 (27%), Positives = 160/345 (46%), Gaps = 8/345 (2%) Query: 36 VKSPVASPVLTHEGETIVIPPASALRDSLKTALIEQRSIAIPFSLPASVEADPARLAKIL 95 V +PVA P + G+ + IP + I + +V A P A I+ Sbjct: 48 VSAPVAPPATSDSGDEVKIPAEYLSVSKITVEAIGSGGLEAEILTAGTVVAAPNSEAVIM 107 Query: 96 PPTTGRIVSIQKRLGDAVKAGDVLFEIESSDLAQAVSDERKAQSTLALTRRNLERQRSLD 155 +G + + ++LGD V+AG+ L ++ S + A +D A + + L R+N R+ SL Sbjct: 108 ARASGNVTQVSRQLGDTVRAGETLAQVSSLEAAAMNADRNVAIARVDLARKNYAREYSLF 167 Query: 156 QANISARRDLEQAQSDYEQADSEAARAHARVAALGGTAASGGSARLAVRAPVSGHVIELN 215 + ++ R+++E AQS A+SEA RA + VA A G S +AV +P+SG + Sbjct: 168 EQGVTPRQEMEAAQSALTVAESEALRATS-VAKAAQVTADGKS--VAVVSPISGKITAQT 224 Query: 216 AAVGSFWNDNTASVMTVADLSSVYVTASAQEKDLARLHIGQDAEIALDAYPGQPLQAKVK 275 +G F + VA V + AS D+ R+ G +A I A G ++A V+ Sbjct: 225 VTLGIFVQPQM-ELFRVAGNGKVLIEASLPVADVTRVASGDNATIL--AANGSSVEATVR 281 Query: 276 SVGQVLDPDTRTVKVRILVTNTDERLKPGMFAQATLR-EQAHAGLLVPMTAVVQSGFANR 334 SV + TRT V + +++ L G QA L + +GL+V AV + Sbjct: 282 SVTPTVSSSTRTAIVVLTPSSSTRELIVGEGVQARLHVKNGASGLVVSEDAVQNVNGHDV 341 Query: 335 VFVEVAPWKFEARKIMLGAQVGNDVEVTDGLQAGTRVVVKDGVLL 379 +FV F + +++G + G ++ G+ AG V ++ L+ Sbjct: 342 LFVRTKE-GFRVQPVLVGTRSGGVAQIISGVSAGEMVATRNAFLV 385 >gi|152981853|ref|YP_001353483.1| CzcB family cobalt/zinc/cadmium efflux transporter membrane fusion protein [Janthinobacterium sp. Marseille] Length = 422 Score = 97.8 bits (242), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 90/309 (29%), Positives = 150/309 (48%), Gaps = 15/309 (4%) Query: 64 LKTALIEQRSIAIPFSLPASVEADPARLAKILPPTTGRIVSIQKRLGDAVKAGDVLFEIE 123 +KTAL+ LP ++ + R A ++P G + S+ LG VK G VL I Sbjct: 105 IKTALV----------LPGEIQFNEDRTAHVVPRLAGVVQSVPANLGQQVKKGQVLAVIA 154 Query: 124 SSDLAQAVSDERKAQSTLALTRRNLERQRSLDQANISARRDLEQAQSDYEQADSEAARAH 183 S+ L++ S+ AQ L L + ER++ L Q ISA +D QAQ + + A Sbjct: 155 STGLSEQRSELFAAQKRLTLAKLTYEREKKLWQEKISAEQDYLQAQQVLHETEIAVQNAR 214 Query: 184 ARVAALGGTAASGGS-ARLAVRAPVSGHVIELNAAVGSFWNDNTASVMTVADLSSVYVTA 242 +++ALG A + G+ R +RAP G V+E + ++G ++ A++ T++DLS+V+ Sbjct: 215 QKLSALGADAGAAGAFNRYEIRAPFDGIVVEKHISLGEAVKED-ANIFTLSDLSTVWADI 273 Query: 243 SAQEKDLARLHIGQDAEIALDAYPGQPLQAKVKSVGQVLDPDTRTVKVRILVTNTDERLK 302 + KDL + +G+ + A+ V VG +L TRT R+ + N + Sbjct: 274 AVPAKDLNAVRVGEKVTVKASAFDSTAT-GTVAYVGSLLGVQTRTANARVTLANPQGTWR 332 Query: 303 PGMFAQATL-REQAHAGLLVPMTAVVQSGFANRVFVEVAPWKFEARKIMLGAQVGNDVEV 361 PG+F + + + V A+ +FV+VA F A+++ G G VE+ Sbjct: 333 PGLFVNVEIVSNEVEVPVAVAADAIQTINDKPVIFVKVAD-GFIAQEVSTGRTDGKRVEI 391 Query: 362 TDGLQAGTR 370 GL+AG+ Sbjct: 392 VKGLKAGSE 400 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.316 0.130 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 705,347 Number of extensions: 25543 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 15 Length of query: 381 Length of database: 1,221,021 Length adjustment: 85 Effective length of query: 296 Effective length of database: 906,776 Effective search space: 268405696 Effective search space used: 268405696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)