BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2027.p (248 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981336|ref|YP_001353751.1| pseudouridylate synthase [Janth... 430 e-122 gi|152981630|ref|YP_001352859.1| RNA pseudouridylate synthase [J... 187 5e-49 gi|152981088|ref|YP_001354188.1| ribosomal large subunit pseudou... 71 7e-14 gi|152980789|ref|YP_001354207.1| pseudouridylate synthase family... 67 7e-13 gi|152981396|ref|YP_001353640.1| ribosomal small subunit pseudou... 67 1e-12 >gi|152981336|ref|YP_001353751.1| pseudouridylate synthase [Janthinobacterium sp. Marseille] Length = 248 Score = 430 bits (1105), Expect = e-122, Method: Compositional matrix adjust. Identities = 207/248 (83%), Positives = 228/248 (91%) Query: 1 MTEPVRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAE 60 M EP+RLSKRMSELGLCSRREADEWI +GWVRVDG+VVS LGSKVLP Q+VTVERQA+AE Sbjct: 1 MNEPLRLSKRMSELGLCSRREADEWIARGWVRVDGKVVSELGSKVLPTQRVTVERQAAAE 60 Query: 61 QAQRVTILINKPMGYVSGQAEDGYKPAVVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRL 120 Q++RVT+LINKPMGYVSGQAEDGYKPAV LVN +RW+EDQ +FHP QL+SLVPAGRL Sbjct: 61 QSKRVTVLINKPMGYVSGQAEDGYKPAVALVNASTRWSEDQAATQFHPTQLRSLVPAGRL 120 Query: 121 DIDSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPEADLKRLNHGLIMDGK 180 DIDSVGLLVLTQDGRIAK LIGED++V+KEYLVRVQY++PGKLPEADLKRLNHGL++DGK Sbjct: 121 DIDSVGLLVLTQDGRIAKHLIGEDTSVEKEYLVRVQYSKPGKLPEADLKRLNHGLMLDGK 180 Query: 181 PLRPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELPTGQWR 240 L PAKV WQNEDQL F LREGKKRQIRRMCDMVGLKVIGLKRVRIG VKLG+LP GQWR Sbjct: 181 KLLPAKVKWQNEDQLSFILREGKKRQIRRMCDMVGLKVIGLKRVRIGNVKLGDLPVGQWR 240 Query: 241 YLRPDEKF 248 YLR DE+F Sbjct: 241 YLREDEQF 248 >gi|152981630|ref|YP_001352859.1| RNA pseudouridylate synthase [Janthinobacterium sp. Marseille] Length = 237 Score = 187 bits (475), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 100/248 (40%), Positives = 149/248 (60%), Gaps = 11/248 (4%) Query: 1 MTEPVRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAE 60 M + +RLSKR++E CSRREA+++IE GWV VDG+V+ G +V P Q + + A+ Sbjct: 1 MVDSIRLSKRVAEALACSRREAEQYIEGGWVSVDGKVIEEPGHRVDPQQAIVLSPDATLV 60 Query: 61 QAQRVTILINKPMGYVSGQAEDGYKPAVVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRL 120 VTIL++KP G + A P ++ +AED++ +RF + L P L Sbjct: 61 AVDPVTILLHKPAGMDAAAALQCITP-------ENLYAEDRSGIRFLKKHTVDLKPTDSL 113 Query: 121 DIDSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPEADLKRLNHGLIMDGK 180 + + GLLV TQD +A++L+ + + +++EY+ V G L L LNHGL M+ K Sbjct: 114 ESLASGLLVFTQDWHVARKLVDDAAGIEQEYIAEVS----GTLVANGLALLNHGLSMNRK 169 Query: 181 PLRPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELPTGQWR 240 PL P KV WQNE +LRF + ++ QI MC+ VGL ++GLKR+R+ + + LP GQWR Sbjct: 170 PLAPVKVSWQNETRLRFAGKGIQRGQIPYMCEQVGLTLVGLKRIRMARMPMAALPVGQWR 229 Query: 241 YLRPDEKF 248 YL+ E+F Sbjct: 230 YLKGYERF 237 >gi|152981088|ref|YP_001354188.1| ribosomal large subunit pseudouridine synthase B [Janthinobacterium sp. Marseille] Length = 546 Score = 70.9 bits (172), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 67/246 (27%), Positives = 122/246 (49%), Gaps = 29/246 (11%) Query: 6 RLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVE----RQASAEQ 61 +L K ++E GL SRR+ +E I G V V+G+ + G ++LP +V + ++ +++ Sbjct: 185 KLHKVLAEAGLGSRRDMEELIIAGRVSVNGEPAHI-GQRILPTDQVRINGKLIQRKVSKK 243 Query: 62 AQRVTILINKPMGYVSGQAEDGYKPAVVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRLD 121 RV ++ +KP G + + +P+V R + + GRLD Sbjct: 244 PPRV-LVYHKPAGEIVSTNDPEGRPSVF--------------DRLPTMKAAKWLAVGRLD 288 Query: 122 IDSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPEADLKRLNHGLIMDGKP 181 ++ GLL+ T G +A +L+ +D+EY VR G+L E ++L G+ ++ Sbjct: 289 FNTEGLLLFTTSGDLANRLMHPRYGIDREYAVRTL----GELEEGMRQKLLAGVELEDGL 344 Query: 182 LRPAKVVWQNEDQL----RFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGE-LPT 236 + +K+ + + R T+ EG+ R++RRM + +GL V L R R G + L + L Sbjct: 345 AQFSKIADGGGEGVNKWYRVTIGEGRNREVRRMFEAIGLTVSRLIRTRYGLLTLPKNLKR 404 Query: 237 GQWRYL 242 G+W + Sbjct: 405 GRWEEM 410 >gi|152980789|ref|YP_001354207.1| pseudouridylate synthase family protein [Janthinobacterium sp. Marseille] Length = 211 Score = 67.4 bits (163), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 56/172 (32%), Positives = 77/172 (44%), Gaps = 37/172 (21%) Query: 101 QTEMRFHPR----------QLKSLVPAGRLDIDSVGLLVLTQDGRIAKQLIGEDSAVDKE 150 QT +F P + ++ PAGRLD DS GLL+L DG++ + D K Sbjct: 35 QTMCQFSPHPTRKSLADHIDIPNIYPAGRLDADSEGLLLLNDDGKLQHAISHPDRKHTKT 94 Query: 151 YLVRVQYTRPGKLPEAD-LKRLNHGLIMDGKPLRPAKV--------VWQNEDQLR----- 196 YLV+V+ +P+AD L RL L + + +V +W +R Sbjct: 95 YLVQVE-----GVPDADALARLRAPLDLGDFVTQECEVKRVAEPDWLWPRNPPIRERANA 149 Query: 197 ------FTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELP--TGQWR 240 TL EGK RQ+RRM VG + L R+ IG V L P G+WR Sbjct: 150 PTSWLAITLSEGKNRQVRRMTGAVGYPTLRLVRIAIGPVSLQSHPLWPGEWR 201 >gi|152981396|ref|YP_001353640.1| ribosomal small subunit pseudouridine synthase A protein [Janthinobacterium sp. Marseille] Length = 238 Score = 66.6 bits (161), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 61/241 (25%), Positives = 94/241 (39%), Gaps = 20/241 (8%) Query: 5 VRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAEQAQR 64 + L + + G +R+ IE G V ++G+ V+ + Q Sbjct: 4 LTLDRILQSQGFGTRKYCRNLIEDGEVSINGESVTDYRAAFDTSDLEFTIFDEVWRYRQH 63 Query: 65 VTILINKPMGYVSGQAEDGYKPAVVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRLDIDS 124 V I++NKP + + + + L+ EQ W E L P GRLD D+ Sbjct: 64 VYIVLNKPANFECSRKPSHHPGVLTLLPEQFTWRE--------------LQPVGRLDHDT 109 Query: 125 VGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPEADLKRLNHGLIMD-GKPLR 183 G+L+++ DG + K Y V T PE + L + D PL Sbjct: 110 TGMLLMSDDGPFIHAQSSPKRHIPKVY---VATTHDPVTPELIAQLLGGVQLHDEPAPLA 166 Query: 184 PAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLG--ELPTGQWRY 241 ++L L +GK Q++RM G L+RV IG + L EL G+W Y Sbjct: 167 AQTCRMLGANELEIVLEQGKYHQVKRMLAAAGNHCSALRRVAIGALTLESLELDEGEWAY 226 Query: 242 L 242 L Sbjct: 227 L 227 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 604,313 Number of extensions: 24831 Number of successful extensions: 69 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 5 Length of query: 248 Length of database: 1,221,021 Length adjustment: 81 Effective length of query: 167 Effective length of database: 921,564 Effective search space: 153901188 Effective search space used: 153901188 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)