BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1824.p (546 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982009|ref|YP_001355036.1| glutathione-regulated potassium... 228 9e-61 gi|152980839|ref|YP_001353999.1| antiporter-2, CPA2 family monov... 192 3e-50 gi|152982025|ref|YP_001353626.1| glutathione-regulated potassium... 166 3e-42 gi|152982911|ref|YP_001352645.1| glutathione-regulated potassium... 107 2e-24 >gi|152982009|ref|YP_001355036.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Janthinobacterium sp. Marseille] Length = 682 Score = 228 bits (580), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 169/518 (32%), Positives = 264/518 (50%), Gaps = 29/518 (5%) Query: 28 LKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLSVKNI 87 + +PP++GYLV GI IGP++ G+ D L E GV+ LMF +GL FSL LL+++ Sbjct: 45 MHLPPMLGYLVVGILIGPYSLGWAEDNATTHALGEFGVVFLMFSIGLEFSLSKLLAMRRT 104 Query: 88 AIPGAVAQIAIATLLGMGLGWM----------LGWPFGEGFLFGLALSVASTAVLLKALQ 137 +AQ+ + M GW+ + W LA+S STA++ K L Sbjct: 105 VFGLGMAQVLLTIGATMVFGWLVAHFSARLSAISWQAALAIGGALAMS--STAIVSKLLT 162 Query: 138 ERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGSAVLLTLGLTLGK 197 ER +E+Q GR +G L+ +D+A+V L+LIPAL+ G G L +A+ K Sbjct: 163 ERLELESQHGRRIIGILLFQDLAVVPLLILIPALANPRGDLGTTLLWAAL---------K 213 Query: 198 VVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAF 257 V + ++L +G ++I + S+ELF L +L I LG A + G+S ALGAF Sbjct: 214 AVIVLFLLLFIGHKLIRSWFRIVVKRRSQELFMLNLLLITLGAAWITEMA-GLSLALGAF 272 Query: 258 FAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVLREPLLVLATVLIIVVG 317 AGM++SE+E H+ E+ RD LFF++VGML + +V+ LVL + +V+ Sbjct: 273 VAGMLISETEYKHQVEEDIKSFRDVLLGLFFITVGMLLNVRLVVEHWWLVLLLLSGVVLL 332 Query: 318 KSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAIL 377 K + R FG P AL +LAQ GEF F+L L+L+ P+ LIL +L Sbjct: 333 KFGLIAFLARCFGAPVGVALRTGLALAQAGEFGFVLLNQVGGLNLVEPLILQLILASMVL 392 Query: 378 SILLNPLLFSLLD----RYEARQ---PKPADVSDVPPTVALQDHVVLVGYGRVGRGIGEQ 430 S+L PL+ + D + A + A T+A Q HV++ G+GR G+ + + Sbjct: 393 SMLAAPLIIAKSDAIVMKLSANEWMVQSLALTQIATRTMATQQHVIIAGFGRSGQSLAKL 452 Query: 431 LRAQGKPFVVIEAQRENLDALRQDGVPALYGNAAHSELLKAAAVERARWLLVAIPEVFEA 490 L A+G + ++ + + + G YG+A E L A + RA L++ A Sbjct: 453 LEAEGITYQALDMDPDRVSEAQAAGASVSYGDAGRRESLVAVGINRAAALVITYASTPSA 512 Query: 491 GQVIENALELNAGLKVVARAHSDAEIEHLEKHGAQRVI 528 +V+ + +L L V+ R+H D +++ L GA V+ Sbjct: 513 MKVLHHMHDLAPNLPVIVRSHDDTDLDKLRAAGASEVV 550 >gi|152980839|ref|YP_001353999.1| antiporter-2, CPA2 family monovalent cation/proton antiporter [Janthinobacterium sp. Marseille] Length = 584 Score = 192 bits (489), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 135/395 (34%), Positives = 214/395 (54%), Gaps = 12/395 (3%) Query: 3 HHTPLIATIVAGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGF--VGDADLAQEL 60 H I + +++A I + H + P ++GY++AG+ IGP TP F + D + + Sbjct: 2 HGAEFIQDLAVIMLIAGIVTIIFHHFRQPIVLGYILAGVIIGPHTPPFELIQDDRTIKIV 61 Query: 61 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWMLGWPFGEGFLF 120 AE+GV+ LMF +GL F+LK L V A A +I + T +G +G W + Sbjct: 62 AELGVVFLMFSLGLEFNLKKLSRVGVTAFIAAFGEIVLMTWIGYEIGRYFEWNTMDSLFL 121 Query: 121 GLALSVASTAVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE 180 G L+++ST +++KAL E L + + G LIVEDI LA+ +I LSGI Sbjct: 122 GAMLAISSTTIIVKALDELGLKHERFAHLIFGILIVEDI---LAIGMIALLSGI------ 172 Query: 181 ALSGSAVLLTLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF 240 A++GS + +T+GK+ F+ V L++G ++P +L +A S E+F + VLA+ GF Sbjct: 173 AMNGSVDAGDVFVTVGKLSLFMIVALVLGLLMVPRLLAYVAKFKSNEVFLITVLALCFGF 232 Query: 241 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIV 300 L S ALGAF G I++E+ + P+RD FS LFFV VG+LF+P+++ Sbjct: 233 CL-LVLQMEYSIALGAFMIGAIMAEARQLNLIERLIAPIRDMFSALFFVGVGLLFNPTVL 291 Query: 301 LREPLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSL 360 + L + + +V+GK A L G+ T++ + L+QIGEFSFI+A+LGL+L Sbjct: 292 VTYALPIALITVAVVLGKVFACGLGTFVAGNDGRTSMRVGMGLSQIGEFSFIIASLGLTL 351 Query: 361 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEAR 395 + S +++ + ++ LL P L D R Sbjct: 352 QVTSEFLYPIVVAVSAVTTLLTPYLIKWADPVSLR 386 >gi|152982025|ref|YP_001353626.1| glutathione-regulated potassium-efflux system protein kefB [Janthinobacterium sp. Marseille] Length = 594 Score = 166 bits (421), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 151/524 (28%), Positives = 258/524 (49%), Gaps = 17/524 (3%) Query: 24 LAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLS 83 L R+ + ++GYL AG+AIGP G + DA +AE+GV++ +F +GL L S Sbjct: 26 LFKRIGLGSVLGYLAAGLAIGPSGLGLMSDAQTIIHVAELGVVMFLFIIGLEMKPSHLWS 85 Query: 84 VKNIAIPGAVAQIAIATLLGMGLGWMLGWPFGEGFLFGLALSVASTAVLLKALQERRLVE 143 ++ Q+ +++L G+G + G+P+ F+ G + STA++++ L ER ++ Sbjct: 86 LRGQIFGLGSMQVVASSILLTGVGIVFGFPWQVSFVAGAGFVLTSTAIVMQVLSERGDIQ 145 Query: 144 TQRGRIAVGWLIVEDIAMV--LALVLIPALSGILGGKGEALSGSAVLLTLGLTLGKVVAF 201 T RG+ V L+ ED+ +V LA+V A + G + + L LT Sbjct: 146 TPRGQRIVSILLFEDLLIVPLLAIVAFLAPADAAATHGAPVLQKIIFAALSLT------- 198 Query: 202 VAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAFFAGM 261 V++ G ++ + +A +RE+ A L + L A + G+S A+GAF AG+ Sbjct: 199 --VLIGAGIWLLNPLFRVLAKAKAREVMTAAALLVVL-GAALLMELGGLSMAMGAFLAGV 255 Query: 262 ILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVLREPLLVLATVLIIVVGKSLA 321 +LSES H+ + P R LFF+ VGM + +V LV+A VL +++ K+ Sbjct: 256 LLSESTFRHQLEADIEPFRGLLLGLFFLGVGMALNLDVVASNWQLVIAGVLALMLAKAFC 315 Query: 322 AWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILL 381 + + R ++ A+ + +AQ GEF+F+L T L L+++S I +LS+ L Sbjct: 316 IYCVARLAKSNHAEAIDRAILMAQGGEFAFVLFTEALKLNVISAEINANITAIVVLSMAL 375 Query: 382 NPLLFSLLDRYEARQPKPADVSDVPPTVALQDHVVLVGYGRVGRGIGEQLRAQGKPFVVI 441 P++ L R+ + D + P V Q V+++G+GRVG+ + A+G +I Sbjct: 376 TPMVVVLHKRFGGKPGVSLDGIEEPRDV--QASVLIIGFGRVGQIASQGPLAKGAKLSII 433 Query: 442 EAQRENLDALRQDGVPALYGNAAHSELLKAAAVERARWLLVAIPEVFEAGQVIENALELN 501 + + + A+ G YG+ ++L AA +A +LV I + +++E A + Sbjct: 434 DNDPDVIRAVEPYGFKVFYGDGGRPDILHAAGALQASVILVCINDKNAVTRIVETAKQEC 493 Query: 502 AGLKVVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLEMVEQA 545 K+V RA L KH A V+ RE L+M +QA Sbjct: 494 PHTKLVVRAFDREHALELVKHDADFVV---RETFEASLQMAKQA 534 >gi|152982911|ref|YP_001352645.1| glutathione-regulated potassium-efflux system protein kefB [Janthinobacterium sp. Marseille] Length = 575 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 131/522 (25%), Positives = 236/522 (45%), Gaps = 35/522 (6%) Query: 30 MPPLIGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLSVKNIAI 89 + P++GY+V G+ + G + LAE+GV+ L+F +GLHFSL + + Sbjct: 37 LSPIVGYIVLGLGLNISGFGLLYGKSTISLLAELGVVFLLFDIGLHFSLDRIRKQASDIF 96 Query: 90 PGAVAQIAIATLLGMGL-GWMLGWPFGEGFLFGLALSVASTAVLLKALQERRLVETQRGR 148 Q+ AT G+ L +LG L G L+++STAV+ + + ER G Sbjct: 97 AFGPFQVIFATT-GLTLTALVLGISIVPALLIGSILALSSTAVVGRLIAERHQQNCPVGL 155 Query: 149 IAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGSAVLLTLGLTLGKVVAFVAVMLIV 208 A LI +D+A + L++ +L G + L L K + A+ + + Sbjct: 156 TATSILIFQDVAAIFLLIVANSLD----------DGGTIWTVAALALCKAILAFAITVAI 205 Query: 209 GRRVIPWILERIADTGSRELFRLAVLAIAL--GFALGSAYVFGVSFALGAFFAGMILSES 266 R +I +L +A + + E+F A L IAL G+A G G+S LGAF G+ L+E+ Sbjct: 206 SRLLIGSLLGLVARSKNEEVFTAAALLIALAAGWATGK---MGLSLTLGAFLGGLALAET 262 Query: 267 ELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVLRE-PLLVLATVLIIVVGKSLAAWLI 325 E P R FF+S+G+ + +++ PL++ T+L+ +V K ++ Sbjct: 263 PYRAVIESEIRPFRGLLLGFFFISIGLSLNVNVLAHSLPLIIGLTILLFIV-KIISNITA 321 Query: 326 VRAFGH--PNSTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILLNP 383 R F P ST L LAQ EF+F++ +L + +I+ LS+ + P Sbjct: 322 SRVFKWSVPGSTQLGF--LLAQGSEFAFVILSLPSMQSAIGDTNTSVIIAVVALSMAVTP 379 Query: 384 LLFSLLDRYEAR-----QPKPADVSDVPPTVALQDHVVLVGYGRVGRGIGEQLRAQGKPF 438 L + + R + K A +++ P + +++VG G +GR I + L + Sbjct: 380 NLADVGRKLAGRMRMRKRVKEALDAELTPKID-NAPIIIVGMGTIGRTIADALIKFEIGY 438 Query: 439 VVIEAQRENLDALRQDGVPALYGNAAHSELLKAAAVERARWLLVAIP--EVFEAGQVIEN 496 +E ++ L+ DG A +G+ ++ + + ++ ++ P ++ + + + N Sbjct: 439 YAVEFDQQRLEMAIADGYDATFGDGFDMQMWGSFKLHERKFSILTTPNFDILDGTRHMAN 498 Query: 497 ALELNAGLKVVARAHSDAEIEHLEKHGAQRVIMGEREIARGM 538 + N LK + E + + G VI +R RG+ Sbjct: 499 SRFPN--LKRYVVVTDEIEAQRFKNIGFATVI--DRRFPRGL 536 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.325 0.142 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,020,077 Number of extensions: 37018 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 4 Length of query: 546 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 458 Effective length of database: 895,685 Effective search space: 410223730 Effective search space used: 410223730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)