BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1568.p (460 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980367|ref|YP_001352036.1| major facilitator superfamily d... 290 1e-79 gi|152982589|ref|YP_001352814.1| major facilitator superfamily m... 117 2e-27 gi|152982562|ref|YP_001352191.1| major facilitator superfamily d... 102 6e-23 gi|152982526|ref|YP_001355122.1| major facilitator superfamily d... 101 8e-23 gi|152982580|ref|YP_001354596.1| major facilitator superfamily d... 72 7e-14 >gi|152980367|ref|YP_001352036.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 478 Score = 290 bits (742), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 170/456 (37%), Positives = 276/456 (60%), Gaps = 2/456 (0%) Query: 7 IPLIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMAD 66 +P +V ALFME +D+T++ T++P++A LH P++LK +++Y+ AL V IPISGWMAD Sbjct: 20 LPWVVAIALFMEQLDSTIINTAIPSMAASLHVTPLSLKAVVSSYILALAVSIPISGWMAD 79 Query: 67 KLGARKVFSAAMIVFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKS 126 + G R+VFS A+ +F SILC + ++ VAAR LQG AMM+PVGR+ + R+ KS Sbjct: 80 RFGTRRVFSVAVGLFTFSSILCGLSVNVPMLVAARILQGFSAAMMMPVGRLTVIRTFPKS 139 Query: 127 ELVKAVSYLTLPALLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFKE 186 EL+ A++++ +PAL+GP++GP +GG I + W+ +F +N+P ++ HR + +++ Sbjct: 140 ELLAAMNFVIIPALIGPLLGPTVGGLIVHWLPWQAIFFVNVPIGLAAQWMIHRHMPDYRS 199 Query: 187 DAPAPLDVKGFVLTSVGASMIMLGLSLFGSPLVDGATPVAMCSIGAIALVMYYRHSTQIP 246 D+P PLDV G VL G +++ L +FG +D + + I L Y H+ + Sbjct: 200 DSPRPLDVIGLVLFGSGVALLSWILEIFGEHYLDATSTTILFIISLALLGAYILHARRTL 259 Query: 247 YPLLDFRLLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFG 306 +PLL L + T SVVGG + R+G+G +PFLLPL Q GLG+ A+Q+G + +A Sbjct: 260 HPLLQLTLFKTRTFRVSVVGGFITRLGVGGLPFLLPLLYQLGLGLPAWQSGLMMMPTAAA 319 Query: 307 SLFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSLHTPYLLIFAIVLMGGVFPAL 366 ++ ++L++ RVL +G+R VL+VN V G+ ++ + I + TP +I + L G F +L Sbjct: 320 AMAMKLISVRVLGHFGYRKVLIVNTVMIGVTISLFSIITPSTPIAVILLLGLAQGFFNSL 379 Query: 367 QFTCLNSIAYADIDNRDAGRVTSLASVIQQLSLGLGVTVAGIV--LQLSNHLQGHTDIVE 424 QF+ +NS+AY+DI+ D+ +++AS +QQ+S+ G+ I+ L + Q V Sbjct: 380 QFSSMNSMAYSDINPEDSSMASTIASSMQQMSMSFGLAFGSIIAAWYLGDLPQSDRAAVG 439 Query: 425 ADFWPAFLVIGLFSVASVPISGKLPHNAGLELSRGK 460 AFL +G ++ S L N G +S+ Sbjct: 440 NALHHAFLTLGGLTIISSLSFWALRPNDGESVSKAH 475 >gi|152982589|ref|YP_001352814.1| major facilitator superfamily multidrug resistance B translocase [Janthinobacterium sp. Marseille] Length = 517 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 94/351 (26%), Positives = 173/351 (49%), Gaps = 27/351 (7%) Query: 8 PLIVGC-----ALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISG 62 PLIVG A+FM +D+++ S+PA++ DL P +T++ A + +P++G Sbjct: 16 PLIVGTLALSLAVFMNVLDSSIANVSIPAISGDLGVSPQQGTWVITSFSVANAISVPLTG 75 Query: 63 WMADKLGARKVFSAAMIVFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARS 122 W+ ++G ++F ++++F+ S LC A S+ +AAR LQG M+P+ + ++ S Sbjct: 76 WLTQRIGQVRLFIGSILLFVLASCLCGFAPSIEILIAARVLQGAVAGPMIPLSQALLLSS 135 Query: 123 VDKSELVKAVSYLTLPALLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIE 182 ++ A+++ + L+ P++GPLLGG+I+ Y W +F +NIP +GL+ A Sbjct: 136 YPPAKSGMALAFWGMTTLVAPIMGPLLGGWISDNYTWPWIFFINIP---IGLFAAWATWS 192 Query: 183 NFKEDAPA----PLDVKGFVLTSVGASMIMLGLSLFGSPL--VDGATPVAMCSIGAIALV 236 +KE P+D G VL + + L L G L + + S+ +A + Sbjct: 193 IYKERESRTYKLPIDKVGLVLLVLWVGSLQLMLDK-GKELDWFHSGEIIILASVAVVAFI 251 Query: 237 MYYRHSTQIPYPLLDFRLLRIPTLHASVVGGSL-FRIGLGAVPFLLPLALQEGLGMTAFQ 295 + +P++D L + V+ S+ + + G++ +LPL LQ +G TA + Sbjct: 252 YFVIWEIGEEHPVVDLSLFKGRNFSGGVIAISVAYGLFFGSL-VILPLWLQTQIGYTATE 310 Query: 296 AGSITCASAFGSLFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSL 346 AG + ++ + + ++L + R V A A+ IFSL Sbjct: 311 AGKVMAPVGIFAIILSPIVGKMLPKIDARWV----------ATTAFLIFSL 351 >gi|152982562|ref|YP_001352191.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 518 Score = 102 bits (253), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 109/410 (26%), Positives = 182/410 (44%), Gaps = 4/410 (0%) Query: 6 LIPLIVGCALFMENMDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMA 65 L+ IV AL + +D TVL +LP L DL + Y + + G + Sbjct: 22 LVLAIVSVALLLIVVDMTVLYAALPRLTADLTASASAKLWIVNIYALTVSGLLLGMGTLG 81 Query: 66 DKLGARKVFSAAMIVFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARS-VD 124 D+LG + +F A ++VF S+ A + S T +A+R L +G AMM+P II + D Sbjct: 82 DRLGHKSLFIAGLVVFGIASLCAAYSPSSATLIASRALLAVGAAMMMPATLSIIRLAFAD 141 Query: 125 KSELVKAVSYLTLPALLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENF 184 ++E A+ A G +GP++GG + Y+ W VFL+N+P + L +A +I Sbjct: 142 ENERAVAIGIWASVAFGGAALGPVVGGLLLEYFWWGSVFLINVPIVLVALPIALAYIPKG 201 Query: 185 KEDAPAPLDVKGFVLTSVGASMIMLGLSLFGSPLVDGATPVAMCSIGAIALVMYYRHSTQ 244 K + P D+ G + G + L G + + +G L ++ R Sbjct: 202 KANKTRPWDLIGSLQIMAGLVGVTYALKEAGKREPAWGIALLVLLVGIFFLAVFARRQQH 261 Query: 245 IPYPLLDFRLLRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASA 304 +P++DF L + +SV + L + + LQ L MT QAG + Sbjct: 262 SAHPMIDFSLFKNTIFSSSVAAAVVAAAALLGMELVFTQRLQLVLDMTPLQAGVFILPLS 321 Query: 305 FGSLFIRLLASRVLRRYGFRTVLMVNAVFAGIALAAYGIFSLHTPYLLIFAIVLMG-GVF 363 + L RVL R G VL ++ + +G+ + AY ++ I ++++G G+ Sbjct: 322 LAAFVAGPLTGRVLPRLGSAYVLSLSLLISGLGMGAYLFLYDAAAWIQIICLLVLGAGIG 381 Query: 364 PALQFTCLNSIAYADIDNRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLS 413 A+ + A +D AG S+ V +L GVT+ G +L L+ Sbjct: 382 AAMTAASGTIMQSAPVDR--AGMAASVEEVSYELGSVFGVTLMGSILSLA 429 >gi|152982526|ref|YP_001355122.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 481 Score = 101 bits (252), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 110/423 (26%), Positives = 197/423 (46%), Gaps = 32/423 (7%) Query: 20 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 79 + +T++ ++P L+R + T ++ A+ + + ++ W+ + G R+ + A+ Sbjct: 34 LSSTIVNVAIPDLSRYFVLGQERAQWVSTGFMLAMTLSMLLTPWLLLRFGLRRTYGGAIC 93 Query: 80 VFMTGSILCAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 139 + + G ++ + + ++ R L+G+ +M P+ ++I R+ D+ E KA+ Sbjct: 94 LLLIGGVVGGFSINYPMLLSMRVLEGLASGVMQPIPAIVILRAFDQHEQGKAMGIFGFGV 153 Query: 140 LLGPVVGPLLGGFITTYYHWRLVFLLNIPFCFLGLYLAHRFIENFK--EDAPAPLDVKGF 197 +L P VGP +GGF+ + WR +F + +P C + L L RF+ ++ D+ PLD KG Sbjct: 154 VLAPAVGPSVGGFLVELFGWRSIFFVVVPPCLVVLVLIQRFLASYTPVPDSDKPLDWKGL 213 Query: 198 VLTSVGASMIMLGLSLFGSPLVDGATP--VAMCSIGAIALVMYYRHSTQIP-YPLLDFRL 254 +L +G S++ L L G + G P V + IG L+ + + T+ PL++ +L Sbjct: 214 LL--IGVSIVSL---LNGVVELRGDMPQAVVLLVIGVATLIGFILYQTRTSAEPLMNMQL 268 Query: 255 LRIPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLA 314 R + ++ +GL +LLP+ +Q G+ QAG + + LA Sbjct: 269 FRHRQFAMGALVAFIYGMGLFGSTYLLPIYMQMGMNYAPSQAGLVLLPAGIVLAVTIPLA 328 Query: 315 SRVLRRYGFRTVLMVNAVF---AGIALAAYGIFSLHTPYLLIFAIVLMGG-----VFPAL 366 R+ RY VL+ +F A AL A G S T YL + A ++G V PAL Sbjct: 329 GRLADRYP-PNVLVAIGLFLLAASFALMANG--SPATSYLALMAWAVIGRIGLGFVLPAL 385 Query: 367 QFTCLNSIAYADIDNRDAGRVTSLASVI---QQLSLGLGVTVAGIVLQLSNHLQGHTDIV 423 + + D G V AS I +QL +GV++ G+VL+ L + Sbjct: 386 SLGAMRGL--------DIGLVAQGASAINFVRQLGGAIGVSLVGVVLEWRIALHQAQSAL 437 Query: 424 EAD 426 D Sbjct: 438 GTD 440 >gi|152982580|ref|YP_001354596.1| major facilitator superfamily drug (bicyclomycin) resistance translocase [Janthinobacterium sp. Marseille] Length = 422 Score = 72.0 bits (175), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 46/167 (27%), Positives = 78/167 (46%), Gaps = 1/167 (0%) Query: 28 SLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSIL 87 + P + L + ++ +TAY+ + V I G ++D G R + ++ VF ++ Sbjct: 43 AFPNIQASLDASALEVQQTLTAYLFSFAVMILWHGALSDAFGRRNIILVSLAVFAVATLA 102 Query: 88 CAAAGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGP 147 CAA S+ R LQGI V +GR II S + +S +T+ + P + P Sbjct: 103 CAAVHSIEYLWVFRMLQGISAGAGVVIGRAIIRDLYAGSAAERLLSLVTMIFSIAPAIAP 162 Query: 148 LLGGFITTYYHWRLVFLLNIPFCFLGL-YLAHRFIENFKEDAPAPLD 193 +LGG+I + +WR +FL + L L Y R E+ + P + Sbjct: 163 ILGGWIVKHSNWRNIFLALFAYAVLLLIYCYKRLPESLPPEKRQPFN 209 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.329 0.143 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,029,286 Number of extensions: 43136 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 12 Length of query: 460 Length of database: 1,221,021 Length adjustment: 86 Effective length of query: 374 Effective length of database: 903,079 Effective search space: 337751546 Effective search space used: 337751546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)