BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1542.p (456 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152982183|ref|YP_001354785.1| major facilitator transporter [... 345 3e-96 gi|152982562|ref|YP_001352191.1| major facilitator superfamily d... 115 4e-27 gi|152980367|ref|YP_001352036.1| major facilitator superfamily d... 109 3e-25 gi|152982589|ref|YP_001352814.1| major facilitator superfamily m... 105 7e-24 gi|152982526|ref|YP_001355122.1| major facilitator superfamily d... 88 1e-18 >gi|152982183|ref|YP_001354785.1| major facilitator transporter [Janthinobacterium sp. Marseille] Length = 469 Score = 345 bits (886), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 193/418 (46%), Positives = 262/418 (62%), Gaps = 1/418 (0%) Query: 13 NHDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSYQIAM 72 DGLP +R AI +V +G+ +A LD +IAN ALP MA L A+ AD++W++N YQ+AM Sbjct: 20 EQDGLPENERRWAIVSVAIGVGMASLDTAIANTALPAMAMQLNATPADSVWIVNIYQLAM 79 Query: 73 AVCLLPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSV 132 LLP A+LGE++GYRR+ + G+ LFT ASLACA + + L +AR QG+GA+ I+ V Sbjct: 80 VATLLPFAALGEILGYRRIAIFGLLLFTAASLACAYAWSLPTLVIARFFQGVGASAIMGV 139 Query: 133 NTALIRFIYPSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGLAAL 192 NTAL+R IYP+R+LGR +G NALV A+GPT A LIL + +W WLF IN+PLGL A Sbjct: 140 NTALLRSIYPARMLGRGLGTNALVVAVAFAVGPTTASLILSVWSWPWLFAINVPLGLFAY 199 Query: 193 AVGHRALPTNPLSARPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWVVGGCTVLTAISLV 252 + + LP + FD +A+ GLL+ I HL A V + + Sbjct: 200 FLARKVLPHTKRATHKFDALTAIYNAGGFGLLILAIGEGAHLSDAKTVALELFGALVFFI 259 Query: 253 LLLRLQS-SSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVMLPFMLQNQQGMSVARA 311 LLLR Q +APMLP+DL R PLF LS T+ C+FAAQ FV LPF + G S Sbjct: 260 LLLRRQKGHAAPMLPVDLFRRPLFTLSALTAVCTFAAQSLAFVSLPFYFETVLGRSAIET 319 Query: 312 GLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLVQPGTPDGVMVAM 371 G L+T W + V + +AG+LSD G+LGG+GL+V+ G++SL+L+ P + Sbjct: 320 GFLITPWAVMVGIMAPIAGRLSDHYPPGMLGGVGLSVLSAGMVSLILMSPDVSVFGICWR 379 Query: 372 FALCGVGFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVGASVVAMLLALSN 429 ALCG+GFG FQ PN R ++S+AP ER G ASG++ T+RL GQ GA++VA +S Sbjct: 380 MALCGIGFGFFQAPNLRAIMSSAPPERGGSASGVVATSRLLGQASGAALVAFCFIIST 437 >gi|152982562|ref|YP_001352191.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 518 Score = 115 bits (289), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 118/424 (27%), Positives = 213/424 (50%), Gaps = 7/424 (1%) Query: 11 HTNHDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSYQI 70 HT+ LP R L +A V + L + V+D ++ ALP + ADL AS + +W++N Y + Sbjct: 11 HTST--LPSSSRWLVLAIVSVALLLIVVDMTVLYAALPRLTADLTASASAKLWIVNIYAL 68 Query: 71 AMAVCLLPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGIL 130 ++ LL + +LG+ +G++ +++ G+ +F +ASL A S L +R L +GAA ++ Sbjct: 69 TVSGLLLGMGTLGDRLGHKSLFIAGLVVFGIASLCAAYSPSSATLIASRALLAVGAAMMM 128 Query: 131 SVNTALIRFIY-PSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGL 189 ++IR + AIG A VA G ALGP V GL+L W +FLIN+P+ L Sbjct: 129 PATLSIIRLAFADENERAVAIGIWASVAFGGAALGPVVGGLLLEYFWWGSVFLINVPIVL 188 Query: 190 AALAVGHRALPTNPLSA-RPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWVVGGCTVLTA 248 AL + +P + RP+D +L M + + Y + G + AW + +L Sbjct: 189 VALPIALAYIPKGKANKTRPWDLIGSLQIMAGLVGVTYALKEAGKREPAWGIALLVLLVG 248 Query: 249 ISL--VLLLRLQSSSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVFVMLPFMLQNQQGM 306 I V R Q S+ PM+ L + +F+ S+ + + AA + + ++ LQ M Sbjct: 249 IFFLAVFARRQQHSAHPMIDFSLFKNTIFSSSVAAAVVAAAALLGMELVFTQRLQLVLDM 308 Query: 307 SVARAGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLVQPGTPDG 366 + +AG+ + +A + G + G++ R+ + + + L + LG+ + L + Sbjct: 309 TPLQAGVFILPLSLAAFVAGPLTGRVLPRLGSAYVLSLSLLISGLGMGAYLFLYDAAAWI 368 Query: 367 VMVAMFALCGVGFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVGASVVAMLLA 426 ++ + L G G G T + T++ +AP +R G A+ + + G G +++ +L+ Sbjct: 369 QIICLLVL-GAGIGAAMTAASGTIMQSAPVDRAGMAASVEEVSYELGSVFGVTLMGSILS 427 Query: 427 LSNA 430 L+ A Sbjct: 428 LAYA 431 >gi|152980367|ref|YP_001352036.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 478 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 88/263 (33%), Positives = 133/263 (50%), Gaps = 9/263 (3%) Query: 29 VVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVINSYQIAMAVCLLPLASLGEVIGY 88 V + L + LD +I N A+P MAA L + V++SY +A+AV + + + G Sbjct: 24 VAIALFMEQLDSTIINTAIPSMAASLHVTPLSLKAVVSSYILALAVSIPISGWMADRFGT 83 Query: 89 RRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALIRFIYPSRLLGR 148 RRV+ V LFT +S+ C S ++ L AR+LQG AA ++ V + +P L Sbjct: 84 RRVFSVAVGLFTFSSILCGLSVNVPMLVAARILQGFSAAMMMPVGRLTVIRTFPKSELLA 143 Query: 149 AIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGLAALAVGHRALPT-NPLSAR 207 A+ + + A G LGPTV GLI+H W +F +N+P+GLAA + HR +P S R Sbjct: 144 AMNFVIIPALIGPLLGPTVGGLIVHWLPWQAIFFVNVPIGLAAQWMIHRHMPDYRSDSPR 203 Query: 208 PFDRQSALLCMCFIGLLLYTIDAVG-HLQGAWVVGGCTVLTAISLVLL----LRLQSSSA 262 P D +L + LL + ++ G H A T+L ISL LL L + + Sbjct: 204 PLDVIGLVLFGSGVALLSWILEIFGEHYLDA---TSTTILFIISLALLGAYILHARRTLH 260 Query: 263 PMLPLDLLRIPLFALSIGTSFCS 285 P+L L L + F +S+ F + Sbjct: 261 PLLQLTLFKTRTFRVSVVGGFIT 283 >gi|152982589|ref|YP_001352814.1| major facilitator superfamily multidrug resistance B translocase [Janthinobacterium sp. Marseille] Length = 517 Score = 105 bits (261), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 112/418 (26%), Positives = 191/418 (45%), Gaps = 27/418 (6%) Query: 24 LAIATVVLGLSI--AVLDGSIANLALPLMAADLKASDADAIWVINSYQIAMAVCLLPLAS 81 L + T+ L L++ VLD SIAN+++P ++ DL S WVI S+ +A A+ + Sbjct: 17 LIVGTLALSLAVFMNVLDSSIANVSIPAISGDLGVSPQQGTWVITSFSVANAISVPLTGW 76 Query: 82 LGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGAAGILSVNTALIRFIY 141 L + IG R+++G + LF LAS C + + L ARVLQG A ++ ++ AL+ Y Sbjct: 77 LTQRIGQVRLFIGSILLFVLASCLCGFAPSIEILIAARVLQGAVAGPMIPLSQALLLSSY 136 Query: 142 PSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIPLGL-AALAV----GH 196 P G A+ + + +GP + G I TW W+F INIP+GL AA A Sbjct: 137 PPAKSGMALAFWGMTTLVAPIMGPLLGGWISDNYTWPWIFFINIPIGLFAAWATWSIYKE 196 Query: 197 RALPTNPLSARPFDRQSALLCMCFIGLLLYTIDAVGHLQGAWV-VGGCTVLTAISLVLLL 255 R T L P D+ +L + ++G L +D L W G +L ++++V + Sbjct: 197 RESRTYKL---PIDKVGLVLLVLWVGSLQLMLDKGKELD--WFHSGEIIILASVAVVAFI 251 Query: 256 RLQ----SSSAPMLPLDLLRIPLFA---LSIGTSFCSFAAQMAVFVMLPFMLQNQQGMSV 308 P++ L L + F+ ++I ++ F + V+LP LQ Q G + Sbjct: 252 YFVIWEIGEEHPVVDLSLFKGRNFSGGVIAISVAYGLFFGSL---VILPLWLQTQIGYTA 308 Query: 309 ARAGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLVQPGTPDGVM 368 AG ++ I + + GK+ +I A + A + L+ + Q +M Sbjct: 309 TEAGKVMAPVGIFAIILSPIVGKMLPKIDARWVATT--AFLIFSLVFFMRSQFTQDVDMM 366 Query: 369 VAMF--ALCGVGFGLFQTPNNRTMISAAPRERTGGASGMLGTARLTGQTVGASVVAML 424 M + G +F P ++S ++ A+G+ R+ +G SV++ + Sbjct: 367 TLMIPTVIQGAAMAMFFIPLTSIILSGQAPDKIPAAAGLSNFVRIMFGGIGTSVMSTM 424 >gi|152982526|ref|YP_001355122.1| major facilitator superfamily drug efflux transporter [Janthinobacterium sp. Marseille] Length = 481 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 100/379 (26%), Positives = 176/379 (46%), Gaps = 14/379 (3%) Query: 7 SLSLHTNHDGLPPRQRLLAIATVVLGLSIAVLDGSIANLALPLMAADLKASDADAIWVIN 66 +L+L D R + L + T ++G A+L +I N+A+P ++ A WV Sbjct: 3 ALTLEELRDRYGARYKWLVLLTCMIGSVAAILSSTIVNVAIPDLSRYFVLGQERAQWVST 62 Query: 67 SYQIAMAVCLLPLASLGEVIGYRRVYLGGVFLFTLASLACAASGDMLQLSLARVLQGLGA 126 + +AM + +L L G RR Y G + L + + S + L RVL+GL + Sbjct: 63 GFMLAMTLSMLLTPWLLLRFGLRRTYGGAICLLLIGGVVGGFSINYPMLLSMRVLEGLAS 122 Query: 127 AGILSVNTALIRFIYPSRLLGRAIGYNALVAGTGNALGPTVAGLILHLATWHWLFLINIP 186 + + +I + G+A+G A+GP+V G ++ L W +F + +P Sbjct: 123 GVMQPIPAIVILRAFDQHEQGKAMGIFGFGVVLAPAVGPSVGGFLVELFGWRSIFFVVVP 182 Query: 187 LGLAALAVGHRALPTN---PLSARPFDRQSALLC-MCFIGLLLYTIDAVGHLQGAWVVGG 242 L L + R L + P S +P D + LL + + LL ++ G + A V+ Sbjct: 183 PCLVVLVLIQRFLASYTPVPDSDKPLDWKGLLLIGVSIVSLLNGVVELRGDMPQAVVLLV 242 Query: 243 CTVLTAISLVLLLRLQSSSAPMLPLDLLRIPLFALSIGTSFCSFAAQMAVF---VMLPFM 299 V T I + L + ++S+ P++ + L R FA+ + +F M +F +LP Sbjct: 243 IGVATLIGFI-LYQTRTSAEPLMNMQLFRHRQFAMG---ALVAFIYGMGLFGSTYLLPIY 298 Query: 300 LQNQQGMSVARAGLLLTAWPIAVALTGVMAGKLSDRIAAGLLGGIGLAVMCLGLLSLLLV 359 +Q + ++AGL+L I +A+T +AG+L+DR +L IGL ++ S L+ Sbjct: 299 MQMGMNYAPSQAGLVLLPAGIVLAVTIPLAGRLADRYPPNVLVAIGLFLLA---ASFALM 355 Query: 360 QPGTPDGVMVAMFALCGVG 378 G+P +A+ A +G Sbjct: 356 ANGSPATSYLALMAWAVIG 374 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.327 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 943,573 Number of extensions: 37194 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 8 Length of query: 456 Length of database: 1,221,021 Length adjustment: 86 Effective length of query: 370 Effective length of database: 903,079 Effective search space: 334139230 Effective search space used: 334139230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)