BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1479.p (546 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligas... 141 8e-35 gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthino... 97 2e-21 gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janth... 74 3e-14 >gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligase [Janthinobacterium sp. Marseille] Length = 560 Score = 141 bits (356), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 157/574 (27%), Positives = 256/574 (44%), Gaps = 54/574 (9%) Query: 2 PQAI--EQTPSAYGYPLLIKQLLHSALATAPEQEIIYGQRRLSYAQFYQRVQRLANALKE 59 P+ I E P+ YG L++ L S A + + L+Y + + L+ Sbjct: 10 PEGIPAEIDPARYGS--LVELLDESFQKYADRNACVCMDKALTYREVDHLSTEVGAWLQS 67 Query: 60 MGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLMN 119 +G+Q G VA+M + +Y AV G V+ VN P ++ + L + A+ +++ Sbjct: 68 IGLQKGARVALMMPNVLQYPIAIAAVLRAGYVIVNVNPLYKPRELEHQLTDSGAEAIIIL 127 Query: 120 SDFMPVLKQIRGELSTLTRCVLLTDDGS---------------------PCPRGPGFAGE 158 +F L+++ ST + V++ G P P A Sbjct: 128 ENFAATLEKVIA--STQVKHVIVATMGDLFGGLKGGIVNFVVRHVKKMVPAYSLPQ-AVP 184 Query: 159 YEQLLAQAEA-RYDFPDFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLATTAGLALP 217 ++++L QA P A YT GTTG KG +HR ++ + L A ++ Sbjct: 185 FKEVLRQAAGMTLQRPALGHQDLAFLQYTGGTTGTAKGAMLTHRNIIANVLQNEAWISPV 244 Query: 218 AQQGRLHRDDVYMPLTPMFHVHAWG-LPYVATMAGVKQVY-PG-RYVPDTICKLIREEKV 274 + +++ P++H+ A + T GV + P R +P I +L++ KV Sbjct: 245 LNTLPKDKQLIFVCALPLYHIFALTTCGMLGTHLGVMNLLIPNPRDIPGFIKELMKY-KV 303 Query: 275 TFSHCVPTILQMILSSEKAREVDLSGWKMIIGGS-AMTSSLARAARE-RGIDVFTGYGMS 332 F V T+ +L++ +VD S K+ GG A+ + RE G + GYG+S Sbjct: 304 HFLPAVNTLYNGLLNNPDFAKVDFSELKLCNGGGMAVQKVVNDKWREVTGCAICEGYGLS 363 Query: 333 ETCPILTLAQVPTAELGGEREASLRVKTGRPVPLVALRTVDGDMNDCAHDGQSTGEVVVR 392 ET P T T E G G PV L+ +D D N+ H S GE+ +R Sbjct: 364 ETSPTATANPPTTNEFTG--------TIGLPVSSTELKIIDSDDNEVPHG--SAGEIAIR 413 Query: 393 APWLTQGYLHQ-AEASEELWRGGWLHTQDIGHIDGEGYLQVTDRIKDVIKTGGEWVSSLQ 451 P + GY + E ++ + G+ T DIG +D G+ ++ DR KD+I G V + Sbjct: 414 GPQVMAGYWNNPVETAQSMTADGFFKTGDIGIMDERGFTKIVDRKKDMILVSGFNVYPSE 473 Query: 452 IEDIIARHPAVAETAVIGVQDGKWGERPVALVVLKKDAVGVSEEDIRQHVRASAEAGVIS 511 IE ++A HP V E A IGV D GE V L V+++D ++E ++ + R + Sbjct: 474 IEGVVAAHPGVLECACIGVPDEHTGEA-VKLFVVRRDP-ALTEAELMAYCREQ-----FT 526 Query: 512 RYGVPDRVRFVDELARTSVGKINKRVMR-EQYPA 544 Y P + F EL +++VGKI +R +R E+ PA Sbjct: 527 GYKKPKSIEFRTELPKSNVGKILRRELRDEKKPA 560 >gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 664 Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 137/549 (24%), Positives = 227/549 (41%), Gaps = 78/549 (14%) Query: 40 RLSYAQFYQRVQRLANALKEMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRL 99 R++Y + Y+RV + AN LKE+G+Q G+ V + S + A +G V Sbjct: 117 RVTYKELYERVCKFANGLKELGIQKGDRVIIYMPMSIEGVAAMQACARIGATHSVVFGGF 176 Query: 100 SPEQIAYTLNHAQADVLLMNSDF-------MPVLKQIRGELSTLTRC------VLLTDDG 146 S + + + A A V ++ SD+ +P LK I E L C V+ G Sbjct: 177 SAKSLQERIIDAGA-VAVLTSDYQVRGGKNLP-LKSIVDEALALGGCDTIRNVVVYKRTG 234 Query: 147 SPCPRGPGFAGEYEQLLAQAEARYDFPDFDENARATTFYTTGTTGLPKGVYFSHRQLVLH 206 G L+ + +A + YT+G+TG PKGV S +L Sbjct: 235 GEMNFVAGRDIWLHDLVDKQKAECEPVPVGAEHPLFILYTSGSTGKPKGVQHSSGGYLLW 294 Query: 207 TLATTAGLALPAQQGRLHRDDVYMPLTPMFHV--HAWGLPYVATMAGVKQV-------YP 257 + T + +DVY + + H + + Y G Q+ +P Sbjct: 295 AMLTMK------WSFDIKPNDVYWCTADIGWITGHTY-VTYGPLACGATQIVFEGVPMFP 347 Query: 258 --GRYVPDTICKLIREEKVTFSHCVPTILQMILSSEKA------REVDLSGWKMIIGGSA 309 GR+ ++++ KVT + PT ++ ++ + A + DLS +++ Sbjct: 348 NAGRF-----WDMVQKHKVTIFYTAPTAIRSLIKAADADPAVHPSKYDLSSLRLL----- 397 Query: 310 MTSSLARAARERGIDVFTGYGMSETCPIL-TLAQVPTAELGGEREASLRVKTGR------ 362 S+ + E CPI T Q E GG + + T Sbjct: 398 --GSVGEPINPEAWMWYYKNIGGERCPIADTFWQT---ETGGHVISPMPGATPMVPGSCT 452 Query: 363 -PVPLVALRTVDGDMNDCAHDGQSTGEVVVRAPW--LTQGYLHQAEASEELW----RGGW 415 P+P +A VD +D A+ G +V++ PW + + + E ++ + GG Sbjct: 453 LPLPGIAAAVVDEVGHDLANG--KGGLLVIKRPWPSMIRNIWNDPERFQKSYFPHELGGT 510 Query: 416 LHTQDIGHIDGE--GYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDG 473 ++ G I E GY +T RI DV+ G + +++IE + +P VAE AV+G D Sbjct: 511 IYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMGTMEIESALVANPIVAEAAVVGKPDE 570 Query: 474 KWGERPVALVVLKKD-AVGVSEEDIRQHVR--ASAEAGVISRYGVPDRVRFVDELARTSV 530 GE A VVLK+ G + I + +R + E G I++ P +RF D L +T Sbjct: 571 TTGESICAFVVLKRSRPTGDEAKQIAKELRDWVAKEIGPIAK---PKEIRFGDNLPKTRS 627 Query: 531 GKINKRVMR 539 GKI +R++R Sbjct: 628 GKIMRRLLR 636 >gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 634 Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 130/535 (24%), Positives = 224/535 (41%), Gaps = 61/535 (11%) Query: 44 AQFYQRVQRLANALKEMGVQAGNTVAVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQ 103 AQ + V R A ++ +GV G+ V + A +G + V + Sbjct: 92 AQLHAEVSRSAAMMQALGVGQGDRVLIYMPMVAEAAFAMLACARIGAIHSVVFGGFASTS 151 Query: 104 IAYTLNHAQADVLLMNSDFMPVLKQIRGELSTLTRC---------------VLLTDDG-S 147 +A ++ A VL++++D RG T +C VL+ D G + Sbjct: 152 LATRIDDATP-VLIISAD-----AGSRGCHVTPYKCLLDEAIALAEHKPAHVLMLDRGLA 205 Query: 148 PCPRGPGFAGEYEQLLAQ-AEARYDFPDFDENARATTFYTTGTTGLPKGVYFSHRQLVLH 206 P G +Y L Q +A + NA + YT+GTTG PKGV + Sbjct: 206 PMKTIEGRDVDYATLRRQHMDAVVPVAWLESNAPSYILYTSGTTGKPKGVLRDVGGYAV- 264 Query: 207 TLATTAGLALPAQQGR--LHRDDVYMPLTPMFHVHAWGLPYVATMAGVKQVYPGRYV-PD 263 LAT+ L + G D+ + + ++ P +A M + +Y G + PD Sbjct: 265 ALATSMDLIFCGKPGETFFCTSDIGWVVGHSYIIYG---PLLAGMTTI--LYEGLPIRPD 319 Query: 264 TIC--KLIREEKVTFSHCVPTILQMILSS--EKAREVDLSGWK-MIIGGSAMTSSLAR-A 317 ++ + KVT PT ++++ E ++ DLS K + + G + + + Sbjct: 320 ASIWWSIVEKYKVTRMSSAPTAIRVLKKQPLELLQKHDLSSLKALYLAGEPLDQTTSDWI 379 Query: 318 ARERGIDVFTGYGMSET-CPILTLAQVPTAELGGEREASLRVKTGRPVPLVALRTVDGDM 376 + G+ V Y +ET PI+++A+ G + + G+P+ + ++ + Sbjct: 380 SGVLGVPVIDNYWQTETGWPIISVAK------GVGDKPTRSGSPGQPMYGYKAKVINEET 433 Query: 377 ND-CAHDGQSTGEVVVRAPWLTQGYLHQAEASEE-----LWRG---GWLHTQDIGHIDGE 427 + CA + + G V++ P L G L ++ WR T D G D + Sbjct: 434 GEECAPNEK--GVVIIEGP-LPPGCLQTLYGDDQRFVSTYWRNFKREAYSTFDWGIRDAD 490 Query: 428 GYLQVTDRIKDVIKTGGEWVSSLQIEDIIARHPAVAETAVIGVQDGKWGERPVALVVLKK 487 GY + R DVI G + + +IE+ I+ H V+E AV+GV++ G+ VA V+LK Sbjct: 491 GYYFILGRTDDVINVAGHRLGTREIEESISSHANVSEVAVVGVENPIKGQCAVAFVILKN 550 Query: 488 DAVGVSEEDIRQHVRASAEAGVISRYGV---PDRVRFVDELARTSVGKINKRVMR 539 A ++ R+ + A V R G P V FV L +T GK+ +R ++ Sbjct: 551 AAAAADPDEARK-LEAEIMKVVDKRLGPIGRPSHVYFVSALPKTRSGKLLRRSIQ 604 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.320 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,245,481 Number of extensions: 50369 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 3 Length of query: 546 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 458 Effective length of database: 895,685 Effective search space: 410223730 Effective search space used: 410223730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)