BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1271.p (898 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980292|ref|YP_001352363.1| pyruvate dehydrogenase subunit ... 1618 0.0 gi|152979821|ref|YP_001353573.1| 2-oxoacid dehydrogenase subunit... 1085 0.0 >gi|152980292|ref|YP_001352363.1| pyruvate dehydrogenase subunit E1 [Janthinobacterium sp. Marseille] Length = 898 Score = 1618 bits (4189), Expect = 0.0, Method: Compositional matrix adjust. Identities = 754/898 (83%), Positives = 833/898 (92%) Query: 1 MSAQIDQVLAQAANDPDVMETQEWLDALEAVIEKEGPDRAHYLMERMVDLARRRGAHIPF 60 MSAQ+DQVLAQAANDPDVMETQEWLDALEAVIE EGPDRAHYLMER++DLARRRG+ +PF Sbjct: 1 MSAQLDQVLAQAANDPDVMETQEWLDALEAVIENEGPDRAHYLMERLIDLARRRGSQVPF 60 Query: 61 SSNTAYVNTIPADQGEHCPGNLEYEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASL 120 SS TAYVNTIPA+ GE+ PGNLEYEERLRSWMRWNAMAMVVKANRVDGDLGGH+SSFASL Sbjct: 61 SSTTAYVNTIPAEMGENSPGNLEYEERLRSWMRWNAMAMVVKANRVDGDLGGHISSFASL 120 Query: 121 ANMLGIGFNHFWHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADGHG 180 ANMLG GFNHFWHAPTEDHGGDLLYIQGHSSPG+YARAFLEGRL+E+QLI+FRRE DG G Sbjct: 121 ANMLGTGFNHFWHAPTEDHGGDLLYIQGHSSPGVYARAFLEGRLSEEQLINFRREVDGKG 180 Query: 181 LSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEM 240 LSSYPHPKLMP+FWQFPTVSMGLGPLMAIYQARFLKYL AR IAKTDNRKVW FCGDGEM Sbjct: 181 LSSYPHPKLMPDFWQFPTVSMGLGPLMAIYQARFLKYLQARDIAKTDNRKVWVFCGDGEM 240 Query: 241 DEPESMGAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVI 300 DE ESMGAIG+AGRE+L+NLV+VVNCNLQRLDGPVRGNGKIIQELE +FRGAGWNV+K+I Sbjct: 241 DEVESMGAIGVAGREQLDNLVMVVNCNLQRLDGPVRGNGKIIQELEGEFRGAGWNVIKII 300 Query: 301 WGSGWDELLAKDKDGILQKVMMETVDGEYQNYKAKDGAYVRKHFFGKHPKLLGMVSKMSD 360 WGS WD LLA+DK+GILQ+VMMET+DGEYQNYKAKDGAYVRKHFFGKHPKLL MVS+MSD Sbjct: 301 WGSNWDPLLARDKEGILQRVMMETLDGEYQNYKAKDGAYVRKHFFGKHPKLLEMVSRMSD 360 Query: 361 DDIWRLTRGGHDPHKIYAAFKVAQESKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLD 420 DD+W L RGGHDPHK+YA FK AQE K QPTV+L K++KGYG GK+GEARNTAH TKKLD Sbjct: 361 DDLWHLLRGGHDPHKVYAGFKQAQEHKGQPTVLLVKTVKGYGMGKSGEARNTAHQTKKLD 420 Query: 421 DEAIRAMRDRFQLPISDAELPNIPFFKPADDAPEMQYLHERRKALGGYLPQRRVHADEKL 480 DEA+R MRDRF LPI+D L +IPFFKPADDAPEM+YLHERR ALGGYLPQRR ADEKL Sbjct: 421 DEAVREMRDRFNLPIADEHLADIPFFKPADDAPEMKYLHERRAALGGYLPQRRQQADEKL 480 Query: 481 PVPELSAFQAMLEPTAEGREISTTAAYSRVLTALLRDPNLGPRVVPIMVDESRTFGMEGL 540 PVPEL+AF+A+L+PTAEGREISTT AY R+++ LLRD NLG R+VPI+VDESRTFGMEGL Sbjct: 481 PVPELAAFKAVLDPTAEGREISTTQAYVRIISILLRDANLGQRLVPILVDESRTFGMEGL 540 Query: 541 FRQIGIFSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRV 600 FRQIGI+++ GQLYEPVDKDQV +YREDKAGQILQEGINEAG M+SWIAAATSYSTNNR+ Sbjct: 541 FRQIGIYNREGQLYEPVDKDQVSFYREDKAGQILQEGINEAGAMASWIAAATSYSTNNRI 600 Query: 601 MIPFYTYYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASA 660 M+PFYT+YSMFGLQRIGDLAWAAGDMRARGFL+GGTAGRTTLNGEGLQHEDGHSH+ AS Sbjct: 601 MVPFYTFYSMFGLQRIGDLAWAAGDMRARGFLLGGTAGRTTLNGEGLQHEDGHSHILAST 660 Query: 661 IPNCIPYDPTFAHEVAVIIHDGLRRMVANQEDVFYYITVMNENYGHPGIKPGQEEGIVKG 720 IPNCIPYDPTFAHEVAVI+HDGL+RM+ NQEDVFYYIT+MNENY PG+KPGQEEGI+KG Sbjct: 661 IPNCIPYDPTFAHEVAVIMHDGLKRMIENQEDVFYYITLMNENYAQPGLKPGQEEGILKG 720 Query: 721 LYLLNEGGKQNQLRVQLLGSGTILREVIAAADLLREDWKVDADVWSAPSFTLLARDGQDV 780 LYLL EG K+ + RVQL+GSGTILREVIAAADLL++DW V ADVWSAPSFTLLARDGQDV Sbjct: 721 LYLLQEGAKKAKNRVQLMGSGTILREVIAAADLLKDDWGVTADVWSAPSFTLLARDGQDV 780 Query: 781 ERWNMLHPAEAPKKSYFEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGRSYKVLGTDGF 840 ERWNMLHP + P++++ SL+G+ GPIVV+TDYMRT+AEQVRAFVPK R+YKVLGTDGF Sbjct: 781 ERWNMLHPTDKPRQAHITSSLQGTAGPIVVATDYMRTFAEQVRAFVPKDRTYKVLGTDGF 840 Query: 841 GRSDTRAKLREFFEVNRYFVTVAALKSLADEGKIKPEVVAQAIAKYGIDPNKPNPVTL 898 GRSD+RAKLREFFEVNRYFVTVAALK+LADEG I VVA+AIAKYGI+P K NPVTL Sbjct: 841 GRSDSRAKLREFFEVNRYFVTVAALKALADEGAIATSVVAEAIAKYGINPEKTNPVTL 898 >gi|152979821|ref|YP_001353573.1| 2-oxoacid dehydrogenase subunit E1 [Janthinobacterium sp. Marseille] Length = 890 Score = 1085 bits (2806), Expect = 0.0, Method: Compositional matrix adjust. Identities = 520/896 (58%), Positives = 675/896 (75%), Gaps = 8/896 (0%) Query: 5 IDQVLAQAANDPDVMETQEWLDALEAVIEKEGPDRAHYLMERMVDLARRRGAHIPFSSNT 64 ++Q A+ DP ETQEWL++L+AVI+ EG RAHYL++++ DL R + T Sbjct: 1 MNQPFAEVDIDPQ--ETQEWLESLDAVIDVEGRPRAHYLLDKLADLDLARHGDLHGRVTT 58 Query: 65 AYVNTIPADQGEHCPGNLEYEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASLANML 124 YVNTI + PG+ E RL +++RWNAM MV++A + ++GGH++++ S A M Sbjct: 59 PYVNTISRQRQPAYPGDAGIERRLNAYIRWNAMVMVLRAGK-HSNVGGHIATYQSAAVMY 117 Query: 125 GIGFNHFWHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADGHGLSSY 184 +GF+HF+ T+ GD++YIQGHS+PGIY RAF+EGR+ E ++ +FRRE+D GLSSY Sbjct: 118 DVGFDHFFRGRTDTFDGDMVYIQGHSAPGIYGRAFIEGRIDEARMDNFRRESDRVGLSSY 177 Query: 185 PHPKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPE 244 PHP+LMP+FWQFPTVSMGLGPL A YQAR+++YL RG+ + RKVWAF GDGEMD+PE Sbjct: 178 PHPRLMPDFWQFPTVSMGLGPLTAAYQARYMRYLEYRGLKQPQGRKVWAFLGDGEMDQPE 237 Query: 245 SMGAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSG 304 S+ AI + GRE+L+NL+ VVNCNLQRLDGPVRGN K+IQELE+ FR AGWNV+KVIWGS Sbjct: 238 SLAAISLGGRERLDNLIFVVNCNLQRLDGPVRGNSKVIQELEATFRAAGWNVIKVIWGSD 297 Query: 305 WDELLAKDKDGILQKVMMETVDGEYQNYKAKDGAYVRKHFFGKHPKLLGMVSKMSDDDIW 364 WD LL KD G+L++ MME VDG++Q +K+++GAYVR+HFFGK+P+LL MV+ MSDD+IW Sbjct: 298 WDRLLEKDVTGLLRRRMMECVDGDFQTFKSQNGAYVRQHFFGKYPELLAMVTDMSDDEIW 357 Query: 365 RLTRGGHDPHKIYAAFKVAQESKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAI 424 +LTRGGHDP K+YAA+ A + QPTVILAK++KG+G G+AGE +N H KK+ +A+ Sbjct: 358 KLTRGGHDPEKVYAAYDQAVRTSGQPTVILAKTVKGFGMGEAGEGQNINHQLKKMSADAV 417 Query: 425 RAMRDRFQLPISDAELPNIPFFKPADDAPEMQYLHERRKALGGYLPQRRVHADEKLPVPE 484 R RDRF LP+SD +L +P+ +PA D+ E +YL RRKALGG++P R E L VP Sbjct: 418 RTFRDRFALPVSDEQLEEMPYLRPAPDSEEARYLVARRKALGGHIPARFSEV-EPLAVPP 476 Query: 485 LSAFQAMLEPTAEGREISTTAAYSRVLTALLRDPNLGPRVVPIMVDESRTFGMEGLFRQI 544 LSAF + L+ + E R +STT ++ R+LTALL+D NLG V+PI+ DESRTFGMEGLFRQI Sbjct: 477 LSAFASQLQSSGE-RGVSTTMSFVRILTALLKDENLGKLVIPIVPDESRTFGMEGLFRQI 535 Query: 545 GIFSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRVMIPF 604 GI S +GQLY P D Q+ YY+E K GQILQEGINE+G +SSWIAA T+Y+ + IPF Sbjct: 536 GIHSYLGQLYTPQDAGQLSYYKEAKDGQILQEGINESGAISSWIAAGTAYANHGLATIPF 595 Query: 605 YTYYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASAIPNC 664 Y +YSMFGLQR+GDLAWAA D R RGFL+G TAGRTTL GEGLQH+DGHSHV +S IP+C Sbjct: 596 YIFYSMFGLQRVGDLAWAAADARTRGFLLGATAGRTTLMGEGLQHDDGHSHVLSSVIPSC 655 Query: 665 IPYDPTFAHEVAVIIHDGLRRMVANQEDVFYYITVMNENYGHPGIKPGQEEGIVKGLYLL 724 I YDPTF++E+AVIIHDGLRRM QED++YYIT++NE+Y HP + G E GI+KGLYLL Sbjct: 656 ISYDPTFSYELAVIIHDGLRRMYVEQEDIYYYITLLNESYQHPAMPDGAEAGILKGLYLL 715 Query: 725 NEGGKQNQ--LRVQLLGSGTILREVIAAADLLREDWKVDADVWSAPSFTLLARDGQDVER 782 E Q +VQL+GSG+ILREVIAAA+LL +D+ + +D+WSA S T + RDG ER Sbjct: 716 REHAAQQDGAPQVQLMGSGSILREVIAAAELLEQDFGIASDIWSATSLTEVRRDGLAAER 775 Query: 783 WNMLHPAEAPKKSYFEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGRSYKVLGTDGFGR 842 WNMLHP E + Y ++ L G GP+V++TDYM+ +Q+R F+ R + LGTDGFGR Sbjct: 776 WNMLHPDEEMRIPYVQECLMGRRGPVVIATDYMKIVGDQIRPFI-NDRRFIALGTDGFGR 834 Query: 843 SDTRAKLREFFEVNRYFVTVAALKSLADEGKIKPEVVAQAIAKYGIDPNKPNPVTL 898 SDTR LR FFEV+R+F+ +AALK LAD+G I + +AI +YGID NK +P + Sbjct: 835 SDTRESLRSFFEVDRHFIVLAALKVLADDGSIPRSKLGEAIQRYGIDINKVDPAAV 890 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 2,276,041 Number of extensions: 101373 Number of successful extensions: 266 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 2 Length of query: 898 Length of database: 1,221,021 Length adjustment: 92 Effective length of query: 806 Effective length of database: 880,897 Effective search space: 710002982 Effective search space used: 710002982 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)