BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.1047.p (286 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981107|ref|YP_001354527.1| molybdopterin/thiamine biosynth... 380 e-107 gi|152980739|ref|YP_001354915.1| thiamine biosynthesis protein T... 66 2e-12 >gi|152981107|ref|YP_001354527.1| molybdopterin/thiamine biosynthesis family protein [Janthinobacterium sp. Marseille] Length = 283 Score = 380 bits (977), Expect = e-107, Method: Compositional matrix adjust. Identities = 198/269 (73%), Positives = 234/269 (86%), Gaps = 14/269 (5%) Query: 9 DIDYERRFGGVARLYGAAALQRFRQARVCVVGVGGVGSWIVEALARSAIGHLTLIDLDNL 68 DID++RRFGG+ARLYG+AAL+RFR A VCV+GVGGVGSW+VEALARSAIG +T+IDLDNL Sbjct: 14 DIDFDRRFGGIARLYGSAALERFRSAHVCVIGVGGVGSWVVEALARSAIGKITMIDLDNL 73 Query: 69 AESNVNRQIHALTGTLGQAKVTALHERISQINPTCVVTEVEDFVDPENVEQMLGGGRFDY 128 AESN+NRQIHALT TLG+AKVTAL ERI+QINP CVVTE+EDF+ +N+++M+G +DY Sbjct: 74 AESNINRQIHALTDTLGKAKVTALAERIAQINPYCVVTEIEDFLSADNLDEMIGAHHYDY 133 Query: 129 VIDAIDNVPAKVAMIAWCRKNGMPMVTIGSAGGQIDPTQIEIRDLCRTEQEPLLAKVRKR 188 +IDAIDNV AK A+IA+CR++G+ +VTIGSAGGQIDPT+IE+ DLCRTEQEPLLAKVRKR Sbjct: 134 IIDAIDNVRAKTALIAYCRQHGLKLVTIGSAGGQIDPTRIEVLDLCRTEQEPLLAKVRKR 193 Query: 189 LRSQHGFPRGTKNKFHIDAVFSTEPLRFPELAEGEACEVVPAEDMDVGADAADSTQAGVT 248 LR++HGFPRGTKNKF IDAVFS+EPLRFPE AE V A D D Q+G+T Sbjct: 194 LRAEHGFPRGTKNKFGIDAVFSSEPLRFPETAE------VCAVDGD--------EQSGIT 239 Query: 249 GLNCAGFGSAMVVTATFGLVAAAHVLKKL 277 GLNCAGFGSAMVVTA+FGLVAAAHVL+KL Sbjct: 240 GLNCAGFGSAMVVTASFGLVAAAHVLRKL 268 >gi|152980739|ref|YP_001354915.1| thiamine biosynthesis protein ThiF [Janthinobacterium sp. Marseille] Length = 250 Score = 66.2 bits (160), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 47/152 (30%), Positives = 80/152 (52%), Gaps = 6/152 (3%) Query: 29 QRFRQARVCVVGVGGVGSWIVEALARSAIGHLTLIDLDNLAESNVNRQIHALTGTLGQAK 88 ++ A V ++G GG+GS LA + +G +TL+D D + +N+ RQI T +GQAK Sbjct: 24 EKLLAAHVLIIGAGGLGSPAAFYLASAGVGTITLVDDDTVDLTNLQRQILHTTQRVGQAK 83 Query: 89 VTALHERISQINPTCVVTEVEDFVDPENVEQMLGGGRFDYVIDAIDNVPAKVAMIAWCRK 148 + + + +INPT V +++ V +++++ V+D DN + A+ C Sbjct: 84 AASGKQTLEEINPTIKVVALQERVSGARLDELVRNATV--VLDCCDNFATRHAVNRACVA 141 Query: 149 NGMPMVTIGSAGGQIDPTQIEIRDLCRTEQEP 180 N +P+V+ A + D QI + D R EQ P Sbjct: 142 NRVPLVS--GAAIKFDG-QISVFDR-RDEQAP 169 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 601,241 Number of extensions: 23244 Number of successful extensions: 76 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 75 Number of HSP's successfully gapped: 2 Length of query: 286 Length of database: 1,221,021 Length adjustment: 82 Effective length of query: 204 Effective length of database: 917,867 Effective search space: 187244868 Effective search space used: 187244868 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)