BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0833.p (585 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979865|ref|YP_001352342.1| dihydroxy-acid dehydratase [Jan... 276 3e-75 gi|152980587|ref|YP_001354001.1| phosphogluconate dehydratase [J... 175 5e-45 >gi|152979865|ref|YP_001352342.1| dihydroxy-acid dehydratase [Janthinobacterium sp. Marseille] Length = 557 Score = 276 bits (705), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 176/516 (34%), Positives = 291/516 (56%), Gaps = 22/516 (4%) Query: 29 RSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKVAEHVRRGIIEAGGFPVEF--P 86 RS G +F KP++G+ N S +TPCN+ +K+A+ + I ++GG P F P Sbjct: 20 RSMYYAMGYEKEDFD-KPMVGVANGHSTITPCNSGLQKLADIAIKTIKDSGGNPQVFGTP 78 Query: 87 VFSNGES---NLRPTAMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASCD 143 S+G S ++++R + + +E ++ G +D V+++ GCDK P ++ + Sbjct: 79 TISDGMSMGTEGMKYSLISREVIADCIETAVNGQWMDGVLVIGGCDKNMPGGMIAMLRTN 138 Query: 144 VPAIVVTGGPMLNGKHQGRDIGSGTVV--WQLSEQVKAGEITIHDFMAAEAGMSRSAGTC 201 VP+I V GG + G +G+D+ T+V ++ + AG ++ DF E SAG+C Sbjct: 139 VPSIYVYGGTIKPGNWKGKDL---TIVSAFEAVGEFTAGRMSQEDFDGIERNACPSAGSC 195 Query: 202 NTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMVWEGLTLSKILTR 261 M TA+TM+ E+LG++LP+++ + D + A S +++ + L I+TR Sbjct: 196 GGMYTANTMSSSFEALGLALPYSSTMANPDDEKVGSAAESARVLIEAIKNDLKPRDIVTR 255 Query: 262 KAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRFLM 321 KA ENA+ A GGSTNAV+H AIA V+ ++D+ R+ + P I DL+PSG+++ Sbjct: 256 KAIENAVAVIMATGGSTNAVLHFLAIAHAAEVEWTIDDFERMRKKVPVICDLKPSGKYVA 315 Query: 322 EEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWD---NVKDAPIYNDEVVRPLAKPL 378 + + AGG+P V++ L +A LL H D +T+ GQT+ + ++ P + V+ P+ L Sbjct: 316 TDLHKAGGIPQVMKVLLDAGLL-HGDCMTITGQTVAEALAHIPSVPRADQHVIHPIKDAL 374 Query: 379 IEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASC 438 E G + IL+GNL+P G V K + ++ G A VF+D + I + A Sbjct: 375 YEQGHLAILKGNLSPEGCVAKITGLKNPVIT--GPARVFDDEYSAMDAIMAN--KIVAGD 430 Query: 439 VLVMKNVGPKGYPGMAEVGNMGLPPKILATGVKDMV-RISDARMSGTAYGTVILHVAPEA 497 VLVM+ +GPKG PGM E+ + ++ G+ + V I+D R SG +G V+ HV+PEA Sbjct: 431 VLVMRYLGPKGGPGMPEM--LAPTSALVGQGLGESVGLITDGRFSGGTWGMVVGHVSPEA 488 Query: 498 AAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRR 533 GG +G+V++GD + +DA+ +QL++ EEE+ RR Sbjct: 489 FVGGLIGLVEEGDSVTIDAHKLLIQLNVPEEEIARR 524 >gi|152980587|ref|YP_001354001.1| phosphogluconate dehydratase [Janthinobacterium sp. Marseille] Length = 642 Score = 175 bits (444), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 151/519 (29%), Positives = 241/519 (46%), Gaps = 44/519 (8%) Query: 43 QGKPVIGICNTWSELTPCNAHFRKVAEHVRRGIIEAGG---FPVEFPVFSNGESNLRP-- 97 Q P I I + ++++ + F + ++ + +AGG F P +G + +P Sbjct: 65 QTAPSIAIVSAYNDMLSAHQPFERFPHIIKDAVRKAGGVAQFAGGVPAMCDGITQGQPGM 124 Query: 98 -TAMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASC-DVPAIVVTGGPML 155 ++ +R+ +M ++ N DA + L CDK P L++GA +PA+ V GPM Sbjct: 125 ELSLFSRDTIAMSTAIALSHNMFDAALYLGVCDKIVPGLVIGALHFGHLPAVFVPAGPMT 184 Query: 156 NGKHQGRDIGSGTVVWQLSEQVKAGEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAE 215 +G + QL Q K G + + AE AGTC GTA++ + E Sbjct: 185 SGMSNPEK----AKIRQLYAQGKIGRDAL---LEAEGQSYHDAGTCTFYGTANSNQMLME 237 Query: 216 SLGVSLPHNAAI-PAVDARRYVLAHLSGMRIVDMVWEGLTLS---KILTRKAFENAIRTN 271 +G+ LP A I P R + AH + R V++ +G + + +++ K NA+ Sbjct: 238 VMGLHLPGAAFITPNTPLRDALTAH-AAQRAVEISAQGKSFTPIGRLVDEKTIVNAVVAL 296 Query: 272 AAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRFLMEEFYYAGGLP 331 A GGSTN +HL A+A G+ ++ D+ + P + + P+G + F+ AGG Sbjct: 297 LATGGSTNHTLHLVAMAKAAGIVIDWNDFDALSAIAPLVARIYPNGDADVNHFHAAGGTG 356 Query: 332 AVLRRLGEADLLPHKDALTVNG---------------QTMWDNVKDAPIYNDEVVRPLAK 376 V+R L +A LL H D LT+ G Q +W N +V+R A Sbjct: 357 FVIRELLDAGLL-HDDVLTILGRGLRAHAAEPFLQGEQVVWQPAASVS-GNTDVLRTAAD 414 Query: 377 PLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDA 436 P DGG+ +L GNL + ++ R A+VF+ + +L+ D Sbjct: 415 PFSGDGGMKLLSGNLGRAVVKVSAVKVQHRIV--RAPAIVFDSQEAFMHAYQAGELNRD- 471 Query: 437 SCVLVMKNVGPKGYPGMAEVGNMGLPPKILATGVKDMVRISDARMSGTAYGTV--ILHVA 494 + V+++ GP+ GM E+ + L + + ++D RMSG A G V +HV+ Sbjct: 472 -FIAVLRHQGPRAN-GMPELHALTPALGSLQDAGRHVALVTDGRMSG-ASGKVPAAIHVS 528 Query: 495 PEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRR 533 PE AGGPLG V+DGD IEL+A G LQ + E E R Sbjct: 529 PEVLAGGPLGRVRDGDIIELNAETGVLQALVPEAEWAAR 567 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,500,167 Number of extensions: 68883 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 2 Length of query: 585 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 497 Effective length of database: 895,685 Effective search space: 445155445 Effective search space used: 445155445 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)