BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0410.p (66 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152983196|ref|YP_001352006.1| thiamine biosynthesis protein T... 78 1e-16 gi|152982020|ref|YP_001352075.1| thiamine biosynthesis protein T... 76 4e-16 >gi|152983196|ref|YP_001352006.1| thiamine biosynthesis protein ThiS [Janthinobacterium sp. Marseille] Length = 67 Score = 77.8 bits (190), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 37/66 (56%), Positives = 49/66 (74%) Query: 1 MQIELNGKPHPLPDGATLDQLIAGLSLAGKAVAVAVNRQVVPSAQWSLRALAEADKVDVV 60 ++IELNG + G + LIA LSL+GKAVAVA+NR++VP QW+ R L AD++D+V Sbjct: 2 LEIELNGAMRQVESGQNVADLIAELSLSGKAVAVAINREIVPRGQWAERQLQAADRIDIV 61 Query: 61 RAIGGG 66 RAIGGG Sbjct: 62 RAIGGG 67 >gi|152982020|ref|YP_001352075.1| thiamine biosynthesis protein ThiS [Janthinobacterium sp. Marseille] Length = 67 Score = 75.9 bits (185), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 32/66 (48%), Positives = 49/66 (74%) Query: 1 MQIELNGKPHPLPDGATLDQLIAGLSLAGKAVAVAVNRQVVPSAQWSLRALAEADKVDVV 60 + ++LNG+ + + DG+T+ L+A L+L GKA+AVA+NR+V+ + WS R L D +D+V Sbjct: 2 ITLQLNGRAYQVEDGSTVSDLVASLALTGKAIAVAINREVLSDSDWSQRRLLATDSIDIV 61 Query: 61 RAIGGG 66 RAIGGG Sbjct: 62 RAIGGG 67 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.316 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 171,457 Number of extensions: 5695 Number of successful extensions: 26 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 26 Number of HSP's successfully gapped: 2 Length of query: 66 Length of database: 1,221,021 Length adjustment: 38 Effective length of query: 28 Effective length of database: 1,080,535 Effective search space: 30254980 Effective search space used: 30254980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 122 (51.6 bits)