BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0330.p (378 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980438|ref|YP_001354686.1| PilB type IV pilus assembly pro... 495 e-141 gi|152981122|ref|YP_001352378.1| general secretion pathway prote... 310 1e-85 gi|152980378|ref|YP_001352030.1| PilT twitching motility protein... 116 2e-27 gi|152980677|ref|YP_001352029.1| PilU twitching motility protein... 107 1e-24 gi|152982669|ref|YP_001353760.1| twitching motility protein PilU... 96 4e-21 >gi|152980438|ref|YP_001354686.1| PilB type IV pilus assembly protein [Janthinobacterium sp. Marseille] Length = 561 Score = 495 bits (1274), Expect = e-141, Method: Compositional matrix adjust. Identities = 233/393 (59%), Positives = 307/393 (78%), Gaps = 19/393 (4%) Query: 5 DTAVAD-DDVPVVRLLHKLLADAVGRGASDLHFEPFESVFRVRLRVDGVLHELAQPPLSL 63 D AD DD P+V+ L K+L DA+ GASDLHFEPFE +R+R RVDGVL ++AQPPL++ Sbjct: 167 DGGTADIDDAPIVKFLQKILVDAINLGASDLHFEPFEKFYRIRFRVDGVLRDIAQPPLAI 226 Query: 64 REKLATRLKILARLDIAEKRLPQDGKMRLALAE-RSVDFRVSTLPTQFGEKIVLRILDTA 122 +EKLA+R+K++++LDI+EKR+PQDG+M+L +++ RS+DFRVSTLPT FGEKIV+RILD + Sbjct: 227 KEKLASRIKVISKLDISEKRVPQDGRMKLVMSKTRSIDFRVSTLPTLFGEKIVMRILDGS 286 Query: 123 QATLSIAELGYEPAQQQALLHAITKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGVNIAT 182 QA + I LGY+P Q++ LL+AI +P+G+VL+TGPTGSGKTVSLY CL LN+PG+NI+T Sbjct: 287 QAQMGIEALGYDPDQKETLLNAIQRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINIST 346 Query: 183 AEDPVEINLPGVNQVSINERTGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQT 242 AEDP EINLPGVNQV++N+R GL F +AL+AFLRQDPD++MVGEIRDLETADIA+KA+QT Sbjct: 347 AEDPAEINLPGVNQVNVNDRAGLTFPVALKAFLRQDPDIIMVGEIRDLETADIAIKAAQT 406 Query: 243 GHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCHCRR--------- 293 GH+V STLHTNDAPATLTRL+NMGV +NIA+SV LI AQRL R+LC C++ Sbjct: 407 GHMVFSTLHTNDAPATLTRLMNMGVAPFNIASSVILITAQRLARRLCSCKQTTVIPDEAL 466 Query: 294 -PAG-------GRPATFVAQGCPACNHTGFRGRTGIYQVMPVSPAMQSLILAQAGTLELA 345 AG G + GC C+ +G++GR GIYQ+MP+S ++ +IL ++E+ Sbjct: 467 LEAGFSEEDLDGTWLPYKPVGCERCSGSGYKGRVGIYQIMPISEEIEGIILNHGTSMEIE 526 Query: 346 RQAQAEGIIDLRRAGLLKVLRGETSVGEILACT 378 QA+ +G+ LR +GL+KV G TS+ EIL CT Sbjct: 527 AQAKKDGVRTLRESGLVKVKLGLTSLEEILGCT 559 >gi|152981122|ref|YP_001352378.1| general secretion pathway protein E [Janthinobacterium sp. Marseille] Length = 599 Score = 310 bits (793), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 164/387 (42%), Positives = 241/387 (62%), Gaps = 19/387 (4%) Query: 11 DDVPVVRLLHKLLADAVGRGASDLHFEPFESVFRVRLRVDGVLHELAQPPLSLREKLATR 70 +D ++ ++ L A + ASD+H EP + +R R+DGVLH++ Q P ++ + R Sbjct: 209 NDQHIINIVDWLWQYAFEQRASDIHLEPKRDLCAIRFRIDGVLHQVYQVPPTVMIAMTAR 268 Query: 71 LKILARLDIAEKRLPQDGKMRLALAE-RSVDFRVSTLPTQFGEKIVLRILDTAQATLSIA 129 +K+L R+D+ EKR PQDG+++ + V+ R+ST+PT FGEK+V+RI D S+ Sbjct: 269 IKLLGRMDVIEKRRPQDGRIKTRTNNGQEVELRLSTMPTAFGEKLVMRIFDPDVVVKSLP 328 Query: 130 ELGYEPAQQQALLHAITKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGVNIATAEDPVEI 189 ELG+ P Q T+PHG++L+TGPTGSGKT +LY L+ L VN+ T EDP+E+ Sbjct: 329 ELGFPPDDAQRWDKLTTRPHGIILVTGPTGSGKTTTLYTTLKALATSEVNVCTVEDPIEM 388 Query: 190 NLPGVNQVSINERTGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLST 249 NQ+ + L FA +R+ +RQDPD++MVGEIRDLETA++A++A+ TGHLVLST Sbjct: 389 VEGSFNQMQVQHGIDLSFADGVRSLMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLST 448 Query: 250 LHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLC-HCRRPAGG----------- 297 LHTNDAP+ + RLL +GVP Y + ++ I+AQRL R LC HC+ P G Sbjct: 449 LHTNDAPSAVMRLLELGVPYYLLETTLIGIMAQRLTRTLCNHCKSPDGEMLDEVWESLSE 508 Query: 298 -----RPAT-FVAQGCPACNHTGFRGRTGIYQVMPVSPAMQSLILAQAGTLELARQAQAE 351 +PA + GCP C TG+RGRTG+Y+++ V+ +I + L +Q+ A+ Sbjct: 509 GSALPKPAVIYRPVGCPECRQTGYRGRTGLYELLTVTQTFSKMITVETDIHALRQQSIAD 568 Query: 352 GIIDLRRAGLLKVLRGETSVGEILACT 378 G+ LR AG LK++ G T+ E+L T Sbjct: 569 GMKPLRLAGALKIIEGTTTAEEVLKVT 595 >gi|152980378|ref|YP_001352030.1| PilT twitching motility protein [Janthinobacterium sp. Marseille] Length = 347 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 90/254 (35%), Positives = 134/254 (52%), Gaps = 20/254 (7%) Query: 19 LHKLLADAVGRGASDLHFEPFESVFRVRLRVDGVLHELAQPPLSLREKLATRLKIL--AR 76 + +LLA +V ASDLH + +RV G + + PPL ++ I+ + Sbjct: 3 ISELLAFSVKNKASDLHLS---AGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQ 59 Query: 77 LDIAEKRLPQDGKMRLALAERSVDFRVSTLPTQFGEKIVLRILDTAQATLSIAELGYEPA 136 + E+RL D + R FRV+ G VLR + + LS+ +L Sbjct: 60 RKVYEERLEIDFSFAIPGLAR---FRVNAFNQDRGAAAVLRTIPSK--ILSLEDLNAPKI 114 Query: 137 QQQALLHAITKPHGLVLMTGPTGSGKTVSLYACLQRLNQ-PGVNIATAEDPVEINLPG-- 193 L KP GLVL+TGPTGSGK+ +L A + +N+ +I T EDP+E Sbjct: 115 FADLAL----KPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIEDPIEFVHDSKK 170 Query: 194 --VNQVSINERTGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLSTLH 251 +NQ + T F ALR+ LR+DPDV++VGE+RDLET +A+ A++TGHLV TLH Sbjct: 171 CLINQREVGPHTH-SFENALRSALREDPDVVLVGELRDLETIRLALSAAETGHLVFGTLH 229 Query: 252 TNDAPATLTRLLNM 265 T+ A T+ R++++ Sbjct: 230 TSSAAKTIDRIIDV 243 >gi|152980677|ref|YP_001352029.1| PilU twitching motility protein [Janthinobacterium sp. Marseille] Length = 378 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 103/373 (27%), Positives = 170/373 (45%), Gaps = 43/373 (11%) Query: 17 RLLHKLLADAVGRGASDLHFEPFESVFRVRLRVDGVLHELAQPPLSLREKLATRLKILAR 76 + ++ LL + + SDL + F ++DG + +++ PL+ + I++ Sbjct: 8 KFMYDLLRLMLSKNGSDLFIT---ADFPPAFKIDGKMTQVSNQPLTAAHTIELARAIMSD 64 Query: 77 LDIAEKRLPQDGKMRLALAERSVD-FRVSTLPTQFGEKIVLRILDTAQATLSIAELGYEP 135 AE ++ A++ + FRVS Q +V+R + TA SI LG Sbjct: 65 KQAAEFEATKE--CNFAISPGGIGRFRVSAFVQQGRVGMVVRTITTA--IPSIEALGVP- 119 Query: 136 AQQQALLHAITKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGV-NIATAEDPVEINLPGV 194 + L + GLV+M G TGSGK+ +L A + N+ +I T EDPVE P Sbjct: 120 ---ETLKDIVMTKRGLVIMVGATGSGKSTTLAAMIGHRNEHSFGHIITIEDPVEYVHPHK 176 Query: 195 NQVSINERTGLD---FALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLSTLH 251 N V G+D + +AL+ LRQ PDV+ +GEIRD ET D A+ ++TGHL L+T+H Sbjct: 177 NCVITQREIGVDTDGWGVALKNTLRQAPDVIFIGEIRDRETMDYAIAFAETGHLCLATMH 236 Query: 252 TNDAPATLTRLLNMGVPA-------YNIAASVSLIVAQRLVRKLCHCRRPAGGRPATFVA 304 N + L R++N P +++ ++ +++QRL+ + G A + Sbjct: 237 ANSSNQALDRIVNF-FPEERRQQLLMDLSLNLKAMISQRLIP----LKDTKGRCVAVEIM 291 Query: 305 QGCPACNHTGFRGRTGIYQVMPVSPAMQSLILAQAGTLELARQAQAEGIIDLRRAGLLKV 364 P + F+G V + M+ + EL Q + + DL AG + Sbjct: 292 LNSPLVSDLIFKG-----DVHEIKEIMKK-------SRELGMQTFDQALFDLYEAGKISY 339 Query: 365 ---LRGETSVGEI 374 LR SV ++ Sbjct: 340 EDALRNADSVNDL 352 >gi|152982669|ref|YP_001353760.1| twitching motility protein PilU [Janthinobacterium sp. Marseille] Length = 374 Score = 95.9 bits (237), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 62/163 (38%), Positives = 92/163 (56%), Gaps = 14/163 (8%) Query: 145 ITKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGV-NIATAEDPVEINLPG----VNQVSI 199 I + GL+L+ G TGSGK+ ++ A L N +I T EDP+E VNQ I Sbjct: 119 IMEKRGLLLVVGSTGSGKSTTIAAMLDHRNTIATGHILTLEDPIEFLFKNKKSIVNQREI 178 Query: 200 NERTGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLSTLHTNDAPATL 259 D +AL+ LRQ PD +++GEIRD ET A+ +Q+GHLV++TLH N++ L Sbjct: 179 GSDAS-DLPIALKNALRQAPDCILIGEIRDKETMSAALAYAQSGHLVVATLHANNSYQAL 237 Query: 260 TRLLNMGVPAYN-------IAASVSLIVAQRLVRKLCHCRRPA 295 TR++N P N +++++ IV+QRL+ + RR A Sbjct: 238 TRIINF-YPIENRQSLLADLSSTLRAIVSQRLIGAIDGRRRAA 279 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 785,137 Number of extensions: 30905 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 5 Length of query: 378 Length of database: 1,221,021 Length adjustment: 85 Effective length of query: 293 Effective length of database: 906,776 Effective search space: 265685368 Effective search space used: 265685368 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)