BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0303.p (592 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152979995|ref|YP_001354711.1| FtsI cell division protein [Jan... 944 0.0 gi|152981159|ref|YP_001351889.1| penicillin-binding protein 2 [J... 129 6e-31 gi|152981042|ref|YP_001355059.1| penicillin-binding protein [Jan... 56 5e-09 >gi|152979995|ref|YP_001354711.1| FtsI cell division protein [Janthinobacterium sp. Marseille] Length = 586 Score = 944 bits (2441), Expect = 0.0, Method: Compositional matrix adjust. Identities = 449/580 (77%), Positives = 515/580 (88%), Gaps = 1/580 (0%) Query: 14 RVAASKGVAFSSNPILQVKLPAWRSRFVLFVLFAAFVALVLRALWLQGVSTDFLQKQGAS 73 R AASKGV FSSNP+L KLPAWRSR VLF++FAAF+ L RALWLQG+ST+FLQKQGAS Sbjct: 7 RTAASKGVPFSSNPVLATKLPAWRSRVVLFLIFAAFLTLAGRALWLQGMSTEFLQKQGAS 66 Query: 74 RYARTLEMPATRGKITDRNGQVLASSVPVKAIWAIPEDVQAAPKDKLVQLAKLLEMSDAE 133 RYARTLE+PATRGKITDRNGQVLASSVPV+A+WAIPEDV +P +KL LAKLL+M + E Sbjct: 67 RYARTLELPATRGKITDRNGQVLASSVPVRAVWAIPEDVLESPPEKLQALAKLLDMPERE 126 Query: 134 LRKKLDSDRSFVYLKRQVEQDTADKIVKLGIPGIETRKEYKRFYPEGEVMAHVVGFTNIE 193 LR+KLDSDR+FVYLKRQVE DT DKI+KLGI GI+TRKEYKR+YPEGEVMAH+VGFTN+E Sbjct: 127 LRRKLDSDRTFVYLKRQVELDTVDKILKLGIAGIDTRKEYKRYYPEGEVMAHIVGFTNVE 186 Query: 194 DAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRDLTLSIDSKIQYIA 253 D GQ+GMELA QKTL GVTGSRRVIKDRLGR+VEDIES+REPHDG+DL LSIDSKIQYIA Sbjct: 187 DKGQEGMELAYQKTLVGVTGSRRVIKDRLGRIVEDIESVREPHDGKDLALSIDSKIQYIA 246 Query: 254 FTQLKEAVEKYKAKAGGIIVVDAKTGEVLALANMPTYNPNDRSELTGAQLRNRVVTDTFE 313 F+ LKEAVE KAKAGGI+V+DAKTGEVLALAN+PTYNPN+RS LTGAQLRNRV+TDTFE Sbjct: 247 FSALKEAVEVNKAKAGGIVVMDAKTGEVLALANLPTYNPNNRSVLTGAQLRNRVITDTFE 306 Query: 314 PGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHGALTVAQIIEKSSNI 373 PGST+KPF+VAL L+T+RVTP+T+ QTAPGKMTIGTATIGD+HA G L+V+Q+IEKSSNI Sbjct: 307 PGSTMKPFSVALGLETNRVTPNTMIQTAPGKMTIGTATIGDAHASGLLSVSQVIEKSSNI 366 Query: 374 GTAKIALGMPAEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWRPIEQATMSYGHGIS 433 GTAKIAL M EMW++FT+VGFGQQPK FPGAVAGRVRPYKSWRPIEQATMSYGHGIS Sbjct: 367 GTAKIALQMEPHEMWDLFTSVGFGQQPKINFPGAVAGRVRPYKSWRPIEQATMSYGHGIS 426 Query: 434 VSLIQLAHAYLIFARNGDIIPLSFTKVTDSPIGQRVISADTALQMRRMLETVVAPGGTAP 493 VSL+Q+ HAY +FAR+GD+IP++F + TD PIG+R+ SA TA QMR MLE P G+AP Sbjct: 427 VSLLQMVHAYTVFARDGDLIPITFQRTTDQPIGERIFSAKTARQMRSMLEMAAGPTGSAP 486 Query: 494 QAQVPGYRVGGKTGTAYKIENGKYVRKYVASFSGIAPMSDPRLIISVMIDEP-QGQHYGG 552 +AQVPGYRV GKTGTAYKIE G+YVRKYV+SF G AP+SDPR+II+VMIDEP G+HYGG Sbjct: 487 RAQVPGYRVAGKTGTAYKIEKGQYVRKYVSSFVGFAPVSDPRIIIAVMIDEPGAGKHYGG 546 Query: 553 PVAGPVFANVAANALRALNVPPDSSVTNIIIPKEPLEESM 592 VA PVFA V ANALR++NV PDS+VTNIIIP +E+M Sbjct: 547 QVAAPVFATVTANALRSMNVAPDSNVTNIIIPPGSEQENM 586 >gi|152981159|ref|YP_001351889.1| penicillin-binding protein 2 [Janthinobacterium sp. Marseille] Length = 637 Score = 129 bits (323), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 147/586 (25%), Positives = 238/586 (40%), Gaps = 99/586 (16%) Query: 40 FVLFVLFAAFVALVLRALWLQGVSTDFLQKQGASRYARTLEMPATRGKITDRNGQVLASS 99 FV+F+LF +A R +WLQ + Q + + RG I DRNG VLA + Sbjct: 23 FVVFILFGCLLA---RFIWLQVYKHNDYVAQAEDNRISVVPVVPNRGLIVDRNGVVLARN 79 Query: 100 VPVKAIWAIPEDVQAAPKDKLVQLAKLLEMSDAELR--KKLDSD-----RSFVYLKRQVE 152 + P + + + +LA L+++ + R KKL + S + ++ E Sbjct: 80 YSAYTLEITPSKINGDLETLIDELATLVDIQPKDRRRFKKLQEESRNLASSPIRVRLTDE 139 Query: 153 QDTADKIVKLGIPGIETRKEYKRFYPEGEVMAHVVGF------------------TNI-- 192 + + PG+E + R YP G V +HV+G+ TN Sbjct: 140 EVARFSAQRYRFPGVEIQARLFRQYPLGSVASHVIGYIGRINQREAKEIDEGDDATNYRG 199 Query: 193 -EDAGQDGMELAAQKTLAGVTGSRRVIKDRLGRVVEDIESIREPHDGRDLTLSIDSKIQY 251 E G++G+E + L G TG V GR + + S G +L LSID ++Q Sbjct: 200 TEHIGKEGVEKNYEDKLHGRTGYEEVEVSAGGRPIRTL-SRTAATPGDNLILSIDIELQ- 257 Query: 252 IAFTQLKEAVEKYKAKAGGIIVVDAKTGEVLALANMPTYNPNDRSELTGAQ--------- 302 K + + + G +I ++ TG+VLA +MPT++PN E Q Sbjct: 258 ------KVVEQAFGDRRGALIAIEPSTGDVLAYVSMPTFDPNLFVEGIDQQNWDALNNSP 311 Query: 303 ---LRNRVVTDTFEPGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSH--A 357 L NR ++ + PGST KPF AL+ + PS + PG G D Sbjct: 312 DHPLLNRPLSGAYPPGSTYKPFMALAALELGKRKPSDAI-SDPGYFWFGNHKFRDDKEGG 370 Query: 358 HGALTVAQIIEKSSNIGTAKIALGMPAEEMWEMFTTVGFGQQP----KFGFPG------- 406 HG + + + I +S + +A + + + + GFGQ+ +F G Sbjct: 371 HGFVDMYKSIVQSCDTYYYMLANDLGVDAIHDFMKPFGFGQKTGIDLRFERTGVLPSTAW 430 Query: 407 -AVAGRVRPYKSWRPIEQATMSYGHGI-SVSLIQLAHAYLIFARNGDIIPLSFTKVTDS- 463 A A R K W E ++ G G S + +QLAHA + A NG ++ K+ ++ Sbjct: 431 KAAAYRKPEQKKWYAGETISIGIGQGYNSFTPLQLAHATAVLANNGVVMKPHLVKMIENG 490 Query: 464 ----------------PIGQRVISADTALQMRRMLETVVAPGGTAPQAQVPGYRVGGKTG 507 P+ Q I ++ + V G A Y GKTG Sbjct: 491 TTRQRTLTVPKESYRIPLKQENIDV-----IKNAMVGVNKEGTGARAFARAEYVSAGKTG 545 Query: 508 TAYKI---ENGKYVRKYV-------ASFSGIAPMSDPRLIISVMID 543 TA I +N KY + A ++ AP +PR+ ++++++ Sbjct: 546 TAQVIAIKKNEKYDASKISDRHHDHALYTAFAPADNPRIALAIIVE 591 >gi|152981042|ref|YP_001355059.1| penicillin-binding protein [Janthinobacterium sp. Marseille] Length = 762 Score = 56.2 bits (134), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 75/290 (25%), Positives = 118/290 (40%), Gaps = 58/290 (20%) Query: 305 NRVVTDTFEPGSTLKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIG--------DSH 356 N V +PGS+ KPF + +L+ ++PST+ AP G T G D Sbjct: 449 NHVTQAWRQPGSSFKPFIYSASLEKG-LSPSTIINDAPISFDAGQ-TGGQPWEPKNYDGK 506 Query: 357 AHGALTVAQIIEKSSNIGTAKIALGMPAEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYK 416 G +T+ + + KS N+ + +I + A+ E T GF A + PY Sbjct: 507 YDGPMTMRKGLAKSKNMISIRILHKIGAKYGQEYITRFGFD-----------ADKNPPY- 554 Query: 417 SWRPIEQATMSYGHGISVSLIQLAHAYLIFARNG-DIIPLSFTKVTDS------------ 463 T++ G G +V+ +Q+A AY +FA G I P +++TD+ Sbjct: 555 -------LTLALGAG-AVTPLQMAAAYSVFANGGYKISPYMISRITDANGKLLSEVKPDM 606 Query: 464 --PIGQRVISADTALQMRRMLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIENGKYVRKY 521 R I A M ML+ VV G + + GKTGT + Sbjct: 607 AGDEANRAIDPRNAFLMDSMLKGVVTSGTAIKAMALKRTDLAGKTGTTND--------SF 658 Query: 522 VASFSGIAPMSDPRLIISVMIDEPQ---GQHYGGPVAGPVFANVAANALR 568 A F+G P + I + D+P+ + GG +A P++ N AL+ Sbjct: 659 DAWFAGYQP--NIVGIAWIGFDQPKNLGNRETGGGLALPIWINYMQKALK 706 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.318 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,296,137 Number of extensions: 51983 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 3 Length of query: 592 Length of database: 1,221,021 Length adjustment: 89 Effective length of query: 503 Effective length of database: 891,988 Effective search space: 448669964 Effective search space used: 448669964 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)