BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0250.p (383 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152981170|ref|YP_001352408.1| Poly-beta-hydroxyalkanoate depo... 91 1e-19 >gi|152981170|ref|YP_001352408.1| Poly-beta-hydroxyalkanoate depolymerase [Janthinobacterium sp. Marseille] Length = 411 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 78/323 (24%), Positives = 137/323 (42%), Gaps = 32/323 (9%) Query: 76 RTLTLREYGKPAAVPTLVVAPYAGHSSVIADYYHGQSLVETLMAHGVSH-VCLTDWHSAS 134 + ++ + Y L+VAP +GH H L +T+ + V H V +TDW A Sbjct: 94 KDMSAKAYAALKQPTILLVAPLSGH--------HATLLRDTVRSLLVEHDVYITDWRDAR 145 Query: 135 E---DMKDLEIDNYLADLCVVIQELGGRVNLVGLCQG-----GWISAMIAARFPPMVQRL 186 + +Y+ + I++LG ++++ +CQ IS M + R P + + + Sbjct: 146 MVPLTEGPFHLHDYIYYIQDFIRQLGPNLHVISVCQPTVPVLAAISLMASERDPHLPKSM 205 Query: 187 VLAGSPIDAGAGDGPLKQMVERTPIRFYEDLV---------RTGGGLMRGSFMLQGWKNM 237 + G PID + + P ++E+ V G + G G+ M Sbjct: 206 TMMGGPIDPRKSPTQVNNLATEKPYSWFENQVIHSVPASYPGFGRKVYPGFLQHAGFIAM 265 Query: 238 HPDEHYFTEHLDLFQHID--DPVYLAKRETFSAWYETTVDLPGRWYLQAIRNIFKDNLLA 295 +P H H D + H+ D + F Y +D+P +YL+ I+ +F+++ L Sbjct: 266 NPGRHA-QSHWDFYMHLREGDNESAEEHRKFYDEYNAVLDMPAEYYLETIKTVFQEHRLP 324 Query: 296 RGKYVALGKQLDLKDIH-CPLYLLAGERDDITTAEQVMNAATLMGTPATHITQRVVP--G 352 G + GK + +DI L+ + GERDDI+ Q A L + A Q+ Sbjct: 325 LGTWEVEGKLVKPQDIKSVALFTIEGERDDISGLGQTHAAQDLCSSIAKSNKQQFTAPQC 384 Query: 353 GHIGLFMGSRTLAETWPQIAQWL 375 GH G+F G + P+I ++ Sbjct: 385 GHYGIFSGRKWREIICPKITDFI 407 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.322 0.136 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 927,414 Number of extensions: 39393 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 96 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 1,221,021 Length adjustment: 85 Effective length of query: 298 Effective length of database: 906,776 Effective search space: 270219248 Effective search space used: 270219248 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)