BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0038.p (568 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthino... 194 1e-50 gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janth... 164 9e-42 gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligas... 141 1e-34 >gi|152980721|ref|YP_001352572.1| acetyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 664 Score = 194 bits (493), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 170/569 (29%), Positives = 261/569 (45%), Gaps = 42/569 (7%) Query: 36 DQQERTPYAWHIPEFFNIAIAACDR-WADGSG-RTAILCEENDGSLTTWSFDQLKTLSDQ 93 D+ Y W N++ DR +G+ + A++ E +DG +T ++ +L + Sbjct: 70 DESNAPFYKWFEDGELNVSYNCLDRHLTNGNADKVALIFESDDGKVTRVTYKELYERVCK 129 Query: 94 FANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSE 153 FAN L E GI +GDR+ IY+ IE V A ++GA +F F +++ R+ ++ Sbjct: 130 FANGLKELGIQKGDRVIIYMPMSIEGVAAMQACARIGATHSVVFGGFSAKSLQERIIDAG 189 Query: 154 AVALVTD---ASGMDKLHLAGEL-PALKVVFCVEPSDIVLPQTT----------DFW--D 197 AVA++T G L L + AL + C ++V+ + T D W D Sbjct: 190 AVAVLTSDYQVRGGKNLPLKSIVDEALALGGCDTIRNVVVYKRTGGEMNFVAGRDIWLHD 249 Query: 198 RLRAASPVLEPVMTRADDAAMIIYTSGTTGKAKGALHAH-RVLLGHLPGVEVSHDSFPQP 256 + EPV A+ I+YTSG+TGK KG H+ LL + ++ S D +P Sbjct: 250 LVDKQKAECEPVPVGAEHPLFILYTSGSTGKPKGVQHSSGGYLLWAMLTMKWSFDI--KP 307 Query: 257 GDRFWTPADWAWIGGLLDVLLPALYHGVA-VLARRLEKF-DAETVFGLLARHQIRNVFFP 314 D +W AD WI G V L G ++ + F +A + ++ +H++ + Sbjct: 308 NDVYWCTADIGWITGHTYVTYGPLACGATQIVFEGVPMFPNAGRFWDMVQKHKVTIFYTA 367 Query: 315 PTALKMLRGTPD------PRSRWPFSLRSVASGGETLGDDLIAWGREALG---VTINEFY 365 PTA++ L D P SLR + S GE + + W + +G I + + Sbjct: 368 PTAIRSLIKAADADPAVHPSKYDLSSLRLLGSVGEPINPEAWMWYYKNIGGERCPIADTF 427 Query: 366 GQTEC-NLVVSSSSQLYPSASGSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRAPDPVM 424 QTE V+S P GS +PG +VDE GH L G+ G + I+ P P M Sbjct: 428 WQTETGGHVISPMPGATPMVPGSCTLPLPGIAAAVVDEVGHDLANGKGGLLVIKRPWPSM 487 Query: 425 FLRYWRNEDATREKF-----AGDFLLTGDLG-NMDEAGYIRYLGRNDDVITSAGYRIGPA 478 W + + ++ + G L GD +E GY GR DDV+ +G+R+G Sbjct: 488 IRNIWNDPERFQKSYFPHELGGTIYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMGTM 547 Query: 479 PIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHAPG---AALVAELQQHVRAQL 535 IE L+ +P V AAVVG D E + AFVVLK G + EL+ V ++ Sbjct: 548 EIESALVANPIVAEAAVVGKPDETTGESICAFVVLKRSRPTGDEAKQIAKELRDWVAKEI 607 Query: 536 AAHEYPRMIAFVDALPTTATGKIMRKTLK 564 P+ I F D LP T +GKIMR+ L+ Sbjct: 608 GPIAKPKEIRFGDNLPKTRSGKIMRRLLR 636 >gi|152980534|ref|YP_001355180.1| propionyl-CoA synthetase [Janthinobacterium sp. Marseille] Length = 634 Score = 164 bits (416), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 152/530 (28%), Positives = 248/530 (46%), Gaps = 65/530 (12%) Query: 86 QLKTLSDQFANALAEAGIGRGDRIGIYLSQRIETVITHLAAYKLGAVTVPLFYLFGPQAI 145 QL + A + G+G+GDR+ IY+ E LA ++GA+ +F F ++ Sbjct: 93 QLHAEVSRSAAMMQALGVGQGDRVLIYMPMVAEAAFAMLACARIGAIHSVVFGGFASTSL 152 Query: 146 AYRLDNSEAVALVTDASGMDKLH-------------LAGELPA--LKVVFCVEPSDIVLP 190 A R+D++ V +++ +G H LA PA L + + P + Sbjct: 153 ATRIDDATPVLIISADAGSRGCHVTPYKCLLDEAIALAEHKPAHVLMLDRGLAPMKTIEG 212 Query: 191 QTTDFWDRLRAASPVLEPVMTRADDA-AMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS 249 + D+ R + PV +A + I+YTSGTTGK KG L R + G+ + S Sbjct: 213 RDVDYATLRRQHMDAVVPVAWLESNAPSYILYTSGTTGKPKGVL---RDVGGYAVALATS 269 Query: 250 HDSF--PQPGDRFWTPADWAWI--------GGLLDVLLPALYHGVAVLARRLEKFDAETV 299 D +PG+ F+ +D W+ G LL + LY G+ + + DA Sbjct: 270 MDLIFCGKPGETFFCTSDIGWVVGHSYIIYGPLLAGMTTILYEGLPI------RPDASIW 323 Query: 300 FGLLARHQIRNVFFPPTALKMLRGTPDP--RSRWPFSLRSVASGGETLGDDLIAWGREAL 357 + ++ ++++ + PTA+++L+ P + SL+++ GE L W L Sbjct: 324 WSIVEKYKVTRMSSAPTAIRVLKKQPLELLQKHDLSSLKALYLAGEPLDQTTSDWISGVL 383 Query: 358 GVTINEFYGQTECNLVVSSSSQLY---PSASGSMGRAVPGHEVQIVDEE-GHLLPRGQVG 413 GV + + Y QTE + S ++ P+ SGS G+ + G++ ++++EE G + G Sbjct: 384 GVPVIDNYWQTETGWPIISVAKGVGDKPTRSGSPGQPMYGYKAKVINEETGEECAPNEKG 443 Query: 414 HIAIRAPDPVMFLR------------YWRNEDATREKFAGDFLLTGDLGNMDEAGYIRYL 461 + I P P L+ YWRN RE ++ T D G D GY L Sbjct: 444 VVIIEGPLPPGCLQTLYGDDQRFVSTYWRN--FKREAYS-----TFDWGIRDADGYYFIL 496 Query: 462 GRNDDVITSAGYRIGPAPIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKEGHA--- 518 GR DDVI AG+R+G IEE + H V AVVGV++ ++ + AFV+LK A Sbjct: 497 GRTDDVINVAGHRLGTREIEESISSHANVSEVAVVGVENPIKGQCAVAFVILKNAAAAAD 556 Query: 519 PGAA--LVAELQQHVRAQLAAHEYPRMIAFVDALPTTATGKIMRKTLKEM 566 P A L AE+ + V +L P + FV ALP T +GK++R++++ + Sbjct: 557 PDEARKLEAEIMKVVDKRLGPIGRPSHVYFVSALPKTRSGKLLRRSIQAI 606 >gi|152980122|ref|YP_001351965.1| long-chain fatty-acid-CoA ligase [Janthinobacterium sp. Marseille] Length = 560 Score = 141 bits (356), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 155/557 (27%), Positives = 240/557 (43%), Gaps = 87/557 (15%) Query: 60 RWADGSGRTAILCEENDGSLTTWSFDQLKTLSDQFANALAEAGIGRGDRIGIYLSQRIET 119 ++AD R A +C D +LT D L T + L G+ +G R+ + + ++ Sbjct: 35 KYAD---RNACVCM--DKALTYREVDHLST---EVGAWLQSIGLQKGARVALMMPNVLQY 86 Query: 120 VITHLAAYKLGAVTVPLFYLFGPQAIAYRLDNSEAVALV---TDASGMDKL--------- 167 I A + G V V + L+ P+ + ++L +S A A++ A+ ++K+ Sbjct: 87 PIAIAAVLRAGYVIVNVNPLYKPRELEHQLTDSGAEAIIILENFAATLEKVIASTQVKHV 146 Query: 168 ------HLAGELPALKVVFCVEPSDIV-----LPQTTDFWDRLR-AASPVLEPVMTRADD 215 L G L V F V + LPQ F + LR AA L+ D Sbjct: 147 IVATMGDLFGGLKGGIVNFVVRHVKKMVPAYSLPQAVPFKEVLRQAAGMTLQRPALGHQD 206 Query: 216 AAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQPGDRFWTPADWAWIGGLLDV 275 A + YT GTTG AKGA+ HR ++ ++ E AWI +L+ Sbjct: 207 LAFLQYTGGTTGTAKGAMLTHRNIIANVLQNE-------------------AWISPVLNT 247 Query: 276 LLPALYHGVAVLARRLEKFDAETVFGLLARHQ-IRNVFFPP------------------- 315 L P + V A L A T G+L H + N+ P Sbjct: 248 L-PKDKQLIFVCALPLYHIFALTTCGMLGTHLGVMNLLIPNPRDIPGFIKELMKYKVHFL 306 Query: 316 ----TALKMLRGTPDPRSRWPFSLRSVASGGETLGDDLI--AWGREALGVTINEFYGQTE 369 T L PD ++ FS + +GG ++ W RE G I E YG +E Sbjct: 307 PAVNTLYNGLLNNPD-FAKVDFSELKLCNGGGMAVQKVVNDKW-REVTGCAICEGYGLSE 364 Query: 370 CNLVVSSSSQLYPSASGSMGRAVPGHEVQIVDEEGHLLPRGQVGHIAIRAPDPVMFLRYW 429 + +++ +G++G V E++I+D + + +P G G IAIR P + YW Sbjct: 365 TSPTATANPPTTNEFTGTIGLPVSSTELKIIDSDDNEVPHGSAGEIAIRGPQ--VMAGYW 422 Query: 430 RNEDATREKFAGD-FLLTGDLGNMDEAGYIRYLGRNDDVITSAGYRIGPAPIEECLMRHP 488 N T + D F TGD+G MDE G+ + + R D+I +G+ + P+ IE + HP Sbjct: 423 NNPVETAQSMTADGFFKTGDIGIMDERGFTKIVDRKKDMILVSGFNVYPSEIEGVVAAHP 482 Query: 489 AVRIAAVVGVKDALRTEVVKAFVVLKEGHAPGAALVAELQQHVRAQLAAHEYPRMIAFVD 548 V A +GV D E VK FVV ++ A AEL + R Q ++ P+ I F Sbjct: 483 GVLECACIGVPDEHTGEAVKLFVVRRD----PALTEAELMAYCREQFTGYKKPKSIEFRT 538 Query: 549 ALPTTATGKIMRKTLKE 565 LP + GKI+R+ L++ Sbjct: 539 ELPKSNVGKILRRELRD 555 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.321 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,414,743 Number of extensions: 62867 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 3 Length of query: 568 Length of database: 1,221,021 Length adjustment: 88 Effective length of query: 480 Effective length of database: 895,685 Effective search space: 429928800 Effective search space used: 429928800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)