BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0003.p (832 letters) Database: BlastDatabases/707/707 3697 sequences; 1,221,021 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|152980048|ref|YP_001351693.1| DNA gyrase subunit B [Janthinob... 1392 0.0 gi|152980258|ref|YP_001352397.1| DNA topoisomerase IV subunit B ... 349 5e-97 >gi|152980048|ref|YP_001351693.1| DNA gyrase subunit B [Janthinobacterium sp. Marseille] Length = 832 Score = 1392 bits (3602), Expect = 0.0, Method: Compositional matrix adjust. Identities = 693/833 (83%), Positives = 752/833 (90%), Gaps = 2/833 (0%) Query: 1 MSSSPADNTNSAQQS-YGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSI 59 MS+ P DN Q + YGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHH VFEVLDNSI Sbjct: 1 MSAIPNDNAPQPQSAQYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHCVFEVLDNSI 60 Query: 60 DEALAGYCSEIHVTIHADNSISITDNGRGIPTGLKMDDKHEPKRSAAEIVMTELHAGGKF 119 DE+LAG+C+EIHVTIH+DNSISITDNGRGIPTG+K DDKHEPKRSAAEIVMTELHAGGKF Sbjct: 61 DESLAGHCTEIHVTIHSDNSISITDNGRGIPTGIKWDDKHEPKRSAAEIVMTELHAGGKF 120 Query: 120 DQNSYKVSGGLHGVGVSCVNALSKKLKLTIRRDGKVHAMEFAKGIVQNRELEMVDGVQVS 179 DQNSYKVSGGLHGVGVSCVN LSK LKLTIRRDGK H MEF +G+ QNRE+E +DG+ VS Sbjct: 121 DQNSYKVSGGLHGVGVSCVNGLSKLLKLTIRRDGKKHEMEFVRGVPQNREVETIDGMVVS 180 Query: 180 PIKVVGESDKRGTEVHFWADEEIFTHVEFHYEILAKRIRELSFLNNGVHIKLSDQRTGKE 239 PIKVVG++DKRGTEVHFWADEEIFT+VEFHY+ILAKRIRELSFLNNGVHIKLSDQRTGKE Sbjct: 181 PIKVVGDTDKRGTEVHFWADEEIFTNVEFHYDILAKRIRELSFLNNGVHIKLSDQRTGKE 240 Query: 240 EDFAFEGGTRGFVEYINKNKSILNPTIFQATGEKQSDQGTLVSVDVSMQWNDAFNEQVLC 299 EDFAFEGGTRGFVEYINK KS+L+PTIFQATGE+ SDQGT ++VDVSMQWNDA+NEQVLC Sbjct: 241 EDFAFEGGTRGFVEYINKAKSVLHPTIFQATGERLSDQGTNITVDVSMQWNDAYNEQVLC 300 Query: 300 FTNNIPQRDGGTHLTGLRAAMTRVINKYIEENDLAKKAKVEISGDDMREGLTCVLSVKVP 359 FTNNIPQRDGGTHLTGLRAAMTRVINKYI+E++ AKKAKVEISGDDMREGLTCVLSVKVP Sbjct: 301 FTNNIPQRDGGTHLTGLRAAMTRVINKYIDEHEFAKKAKVEISGDDMREGLTCVLSVKVP 360 Query: 360 EPKFSSQTKDKLVSSEVRGPVEEIVAKTLTDFLAEKPNDAKIICGKIVEAARAREAARKA 419 EPKFSSQTKDKLVSSEVRGPVEEIVAKTL D+L EKPNDAKIICGKIVEAARAREAARKA Sbjct: 361 EPKFSSQTKDKLVSSEVRGPVEEIVAKTLADYLQEKPNDAKIICGKIVEAARAREAARKA 420 Query: 420 RELTRRKGVMDGLGLSSKLADCQERDPALCELYIVEGDSAGGSAKQGRDRKFQAILPLRG 479 R+LTRRKGVMDGLGLS+KLADCQE+DPALCELY+VEGDSAGGSAKQGRDRKFQAILPLRG Sbjct: 421 RDLTRRKGVMDGLGLSAKLADCQEKDPALCELYVVEGDSAGGSAKQGRDRKFQAILPLRG 480 Query: 480 KVLNVEKARFEKMLSSEQITTLIATLGTSIGADEFNPDKLRYHRIIIMTDADVDGAHIRT 539 KVLNVEKARFEKML+SEQITTLIATLGTSIGADEFN +KLRYHRIIIMTDADVDGAHIRT Sbjct: 481 KVLNVEKARFEKMLASEQITTLIATLGTSIGADEFNIEKLRYHRIIIMTDADVDGAHIRT 540 Query: 540 LLLTLFYRQMPALVERGHIYIAQPPLYKVKAGKDERYLKDDFEEAQYMMQIALNDAEIKP 599 LLLTL YRQMP LVERGH+YIAQPPLYKVK GKDERYLKDD EEA YMMQIALNDA + P Sbjct: 541 LLLTLLYRQMPQLVERGHVYIAQPPLYKVKHGKDERYLKDDVEEAVYMMQIALNDAALIP 600 Query: 600 AEDAAPISGEALVELARQYNTANAIIMRLSRAIDESALNAIMTGGVALKLENLEQATATA 659 +E A PI+G+AL EL RQYN ANAII RL+RA+D +AL AIMT GV L L ++E A A+A Sbjct: 601 SEGAQPIAGDALAELVRQYNMANAIINRLTRAVDGAALTAIMT-GVTLDLSSVEAAQASA 659 Query: 660 EELKKAINDINIDVQARVDEHSEKVALHIHRRQHGNIKATVIDADFVSGPDYLQLSNAAE 719 + L++ IN+ + V + DE S K L I+R HGN+KA+ ID+DFV+GPDY L+ AA Sbjct: 660 QRLEQGINEPAVTVTVKSDELSGKHTLRINRMYHGNVKASSIDSDFVTGPDYTVLAAAAA 719 Query: 720 TFKGLIGKGAVVARGQGEKRKESVITEFRQAMAWLRDEAERGVNKQRYKGLGEMNPEQLW 779 TFKGLIG GA+V RG GEK K S I++F QAM WLR EAER V KQRYKGLGEMNPEQLW Sbjct: 720 TFKGLIGPGALVQRGAGEKAKVSAISDFHQAMNWLRSEAERNVGKQRYKGLGEMNPEQLW 779 Query: 780 ETTMDPTVRRLLKVQIEDAIAADQIFMTLMGDDVEPRRAFIESNALQAGNIDV 832 ETTMDP VRRLLKVQIEDAIAADQIF TLMGDDVEPRRAFIE NAL AGNID+ Sbjct: 780 ETTMDPAVRRLLKVQIEDAIAADQIFTTLMGDDVEPRRAFIELNALSAGNIDI 832 >gi|152980258|ref|YP_001352397.1| DNA topoisomerase IV subunit B [Janthinobacterium sp. Marseille] Length = 661 Score = 349 bits (895), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 221/607 (36%), Positives = 339/607 (55%), Gaps = 45/607 (7%) Query: 9 TNSAQQSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGYCS 68 T + Y SSI++L+GLE V++RPGMY T LH ++ EV+DN+ DEAL GYC Sbjct: 3 TKKSSADYSESSIRVLKGLEPVKQRPGMY---TRTENPLH-IIQEVIDNASDEALGGYCK 58 Query: 69 EIHVTIHADNSISITDNGRGIPTGLKMDDKHEPKRSAAEIVMTELHAGGKFDQNS---YK 125 I VT++ D S+S+ D+GRGIP GL E K EIV T LHAGGKFD+ S Y Sbjct: 59 NIVVTLNTDGSVSVEDDGRGIPVGLH----PEEKVPTVEIVFTRLHAGGKFDKGSGGAYA 114 Query: 126 VSGGLHGVGVSCVNALSKKLKLTIRRDGKVHAMEFAKGIVQNRELEMVDGVQVSPIKVVG 185 SGGLHGVGVS NALS +L++T+ R+ VH++ FA G ++V+ ++ P+ Sbjct: 115 FSGGLHGVGVSVTNALSTRLEITVWRENGVHSLAFAGG-------DVVEPLKSRPL--TR 165 Query: 186 ESDKRGTEVHFWADEEIFTHVEFHYEILAKRIRELSFLNNGVHIKLSDQRTGKEEDFAFE 245 + + GT V W D + F L + +R + L GV + L ++G+ + + ++ Sbjct: 166 DEKRSGTRVTAWPDAKYFDSSVIPQAELQRLLRSKAVLLPGVKVTLVHAKSGETQTWQYD 225 Query: 246 GGTRGFV-EYINK--NKSILNPTIFQATGEKQSDQGTLVSVDVSMQWNDAFNEQVLC--- 299 G RG++ E + + N L P +F+ +D + W A+ E Sbjct: 226 QGLRGYLTESLAQASNSETLIP-LFEGEQYAGADAEGFAEGE-GAAWVVAWTEDGAVVRE 283 Query: 300 -FTNNIPQRDGGTHLTGLRAAMTRVINKYIEENDLAKKAKVEISGDDMREGLTCVLSVKV 358 + N IP +GGTH +GLR + + ++E + L K V++ +D+ ++ VLS KV Sbjct: 284 SYVNLIPTSNGGTHESGLREGLFGAVKSFVEMHSLLPKG-VKLLPEDVFARVSFVLSAKV 342 Query: 359 PEPKFSSQTKDKLVSSEVRGPVEEIVAKTLTDFLAEKPNDAKIICGKIVEAARAREAARK 418 +P+F Q K++L S + V +L +L + K + +++ A++R + + Sbjct: 343 LDPQFQGQIKERLNSRDAVRLVASFTRPSLELWLNQHVEYGKKLAELVIKQAQSRLRSAQ 402 Query: 419 ARELTRRKGVMDGLGLSSKLADCQERDPALCELYIVEGDSAGGSAKQGRDRKFQAILPLR 478 E + GV L KL DC+ D + EL++VEGDSAGGSAK GRD++FQAILPLR Sbjct: 403 KVEKKKSSGVAV---LPGKLTDCESTDISRNELFLVEGDSAGGSAKMGRDKEFQAILPLR 459 Query: 479 GKVLNVEKARFEKMLSSEQITTLIATLGTSIGA--DEFNPDKLRYHRIIIMTDADVDGAH 536 GKVLN + +++ ++ +I + +G D + LRY RI I++DADVDG+H Sbjct: 460 GKVLNSWETERDRLFANNEIHDIAVAIGVDPHGKNDTVDLSNLRYGRICILSDADVDGSH 519 Query: 537 IRTLLLTLFYRQMPALVERGHIYIAQPPLYKVKA-GKDERYLKDDFEEAQYMMQIALNDA 595 I+ LLLTLF++ P L++RGHI IA+PPLY+V A + ++ ++ + AL+D Sbjct: 520 IQVLLLTLFFKHFPQLIDRGHICIARPPLYRVDAPARGKKPVQKIY---------ALDDG 570 Query: 596 EIKPAED 602 E++ ED Sbjct: 571 ELEAIED 577 Score = 58.2 bits (139), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 32/65 (49%), Positives = 41/65 (63%), Gaps = 3/65 (4%) Query: 766 RYKGLGEMNPEQLWETTMDPTVRRLLKVQIE--DAIAADQIFMTLMGD-DVEPRRAFIES 822 R+KGLGEMN EQLWETTM+P RRLL V + D ++ F LMG + RR +IE Sbjct: 593 RFKGLGEMNAEQLWETTMNPDTRRLLPVSLGEFDLDFSEARFNMLMGKGEAAARRTWIEE 652 Query: 823 NALQA 827 + +A Sbjct: 653 HGNEA 657 Database: BlastDatabases/707/707 Posted date: Apr 13, 2011 12:58 PM Number of letters in database: 1,221,021 Number of sequences in database: 3697 Lambda K H 0.316 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3697 Number of Hits to DB: 1,829,695 Number of extensions: 75076 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 3 Length of query: 832 Length of database: 1,221,021 Length adjustment: 91 Effective length of query: 741 Effective length of database: 884,594 Effective search space: 655484154 Effective search space used: 655484154 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)