BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4680.p (503 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde deh... 821 0.0 gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrog... 207 6e-55 gi|134095327|ref|YP_001100402.1| multifunctional: transcriptiona... 139 5e-34 >gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde dehydrogenase, oxidoreductase protein [Herminiimonas arsenicoxydans] Length = 506 Score = 821 bits (2120), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/498 (79%), Positives = 451/498 (90%) Query: 6 IDHYIQGQRSRSESGRSQDVFNPATGEVTAKVALASVAEVHAAVAAASAAAPAWAETAPL 65 I+HYI G + + G Q VFNPATGE++A V LAS A+V AVAAA AAAPAWAE APL Sbjct: 9 IEHYINGNLTNTVGGGQQPVFNPATGEISANVVLASDADVQTAVAAAKAAAPAWAEMAPL 68 Query: 66 KRARVLFKFKELIEQHHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQF 125 KRAR+LFKFK+LIE+HH+ LA AIT EHGKVFSDAKGEVTRGLE+VEFA G+PQLLKTQF Sbjct: 69 KRARILFKFKDLIERHHNDLAAAITREHGKVFSDAKGEVTRGLEVVEFACGVPQLLKTQF 128 Query: 126 TDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSL 185 +DNIGGGIDNWNLRQPLGVTAGITPFNFP MVP+WMAP+A+ATGN+F+LKPSERDPSPSL Sbjct: 129 SDNIGGGIDNWNLRQPLGVTAGITPFNFPFMVPMWMAPVALATGNTFILKPSERDPSPSL 188 Query: 186 MLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATERAGT 245 M+AEL +AGLPDGVFNV+QGDK AVD LL+HP+++AVSFVGSTPIAEYIY+ R G+ Sbjct: 189 MVAELLHQAGLPDGVFNVLQGDKRAVDGLLQHPDIKAVSFVGSTPIAEYIYQAGAARKGS 248 Query: 246 FPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADRLVE 305 +PLR QALGGAKNHLVVMPDANL+QAVDALIGAAYGSAGERCMAISVAVAVGSVAD+LV Sbjct: 249 YPLRVQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVA 308 Query: 306 ALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAQLLVDGRGLKVPGHE 365 ALIPRVK+LKVKNGME DAEMGP+V+AAHKAK+EGYI++G+ +GA+LLVDGRGL V GH Sbjct: 309 ALIPRVKSLKVKNGMESDAEMGPVVSAAHKAKIEGYIEAGIKSGAKLLVDGRGLTVDGHS 368 Query: 366 EGFFIGGTLFDHVTPDMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTADG 425 GFF+GG LFD+VTP+M IYQEEIFGPVLCVVRVPDF++A+ LINAHE+GNGV+LFT+DG Sbjct: 369 NGFFLGGCLFDNVTPEMTIYQEEIFGPVLCVVRVPDFSAALDLINAHEFGNGVALFTSDG 428 Query: 426 NTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKSIMQ 485 NTA EFSRR+++GMVGINVPIPVPMAWHSFGGWKRS+FGDTHAYGEEGI+FYTRYKSIMQ Sbjct: 429 NTAREFSRRVEIGMVGINVPIPVPMAWHSFGGWKRSMFGDTHAYGEEGIKFYTRYKSIMQ 488 Query: 486 RWSASIGKGAEFTMPVAK 503 RW SI KGAEF MP +K Sbjct: 489 RWPESISKGAEFAMPTSK 506 >gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrogenase I, NADP-dependent [Herminiimonas arsenicoxydans] Length = 492 Score = 207 bits (528), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 143/446 (32%), Positives = 219/446 (49%), Gaps = 15/446 (3%) Query: 24 DVFNPATGEVTAKVALASVAEVHAAVAAASAAAPAWAETAPLKRARVLFKFKELIEQHHD 83 D+ NPA G + E A+ AA +A PAWA +RA ++ ++ +LI + D Sbjct: 35 DISNPADGSIIGSAPNMGAEETETAIRAAQSAFPAWAAKTGKERAIIMRQWFDLIIANVD 94 Query: 84 ALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDNIGGGIDNWNLRQPLG 143 LA +T E GK +++GEV G +E+ + + + + ++QP+G Sbjct: 95 DLAILMTAEQGKPLIESRGEVIYGASFIEWFSEEAKRVSGDVMASTWNDKRTLTIKQPIG 154 Query: 144 VTAGITPFNFPV-MVPLWMAPIAIATGNSFVLKPSERDPSPSLMLAELFKRAGLPDGVFN 202 + A ITP+NFP+ M+ +AP A+A G + V+KP+E+ P +L +AEL RAGLP GV N Sbjct: 155 ICAAITPWNFPIAMITRKVAP-AVAAGCTIVIKPAEQTPLSALAIAELAHRAGLPAGVIN 213 Query: 203 VVQGDK----VAVDALLEHPEVQAVSFVGSTPIAEYIYRKATERAGTFPLRAQALGGAKN 258 V+ D V L P V+ VSF GSTP+ + + A T A LGG Sbjct: 214 VITADAENSIVVGKTLCASPIVRHVSFTGSTPVGRILMEQC---APTVKKIALELGG-HA 269 Query: 259 HLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADRLVEALIPRVKALKVKN 318 +V DA+LD AV + + Y +AG+ C+ + S+ D V+ L +KV N Sbjct: 270 PFIVFDDADLDAAVTGALQSKYRNAGQTCVCTNRFYVHESIYDVFVQKLAAGAARIKVGN 329 Query: 319 GMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAQLLVDGRGLKVPGHEEGFFIGGTLFDHV 378 G E GPL+ KVE ++ V GA ++ G+ + GH F T+ ++ Sbjct: 330 GFEQGVLQGPLIDDQAIRKVEQHVSDAVKKGASVMTGGKKHALGGH----FYEPTVLSNI 385 Query: 379 TPDMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTADGNTAHEFSRRIQVG 438 T DM I EE FGPV +V+ A+ N ++G + D + + +++ G Sbjct: 386 TDDMSIMHEETFGPVAAIVKFKTEDEAIAAANNTDFGLASYFYARDISRVWRVAEKLEYG 445 Query: 439 MVGINVPIPVPMAWHSFGGWKRSLFG 464 MVGIN + + FGG K+S G Sbjct: 446 MVGINTGL-ISNEVAPFGGVKQSGLG 470 >gi|134095327|ref|YP_001100402.1| multifunctional: transcriptional repressor of proline utilization; proline dehydrogenase; pyrroline-5-carboxylate dehydrogenase [Herminiimonas arsenicoxydans] Length = 1224 Score = 139 bits (349), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 118/430 (27%), Positives = 197/430 (45%), Gaps = 34/430 (7%) Query: 27 NPAT-GEVTAKVALASVAEVHAAVAAASAAAPAWAETAPLKRARVLFKFKELIEQHHDAL 85 NPA +V A+ +V A+A A A A W +TAP +RA +L EL +QH Sbjct: 584 NPADHSDVLGTAVEANADDVERALALAIAGAEKWQKTAPSERAGILLNAAELFQQHAPEF 643 Query: 86 AKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDNIGGGIDNWNLRQPLGVT 145 E GK +A GE+ ++ + + + I + Q LG+ Sbjct: 644 IALSVREAGKTLPNAIGELRETIDFLRYYSA---------------QITTAQMEQALGLV 688 Query: 146 AGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSLMLAELFKRAGLPDGVFNVVQ 205 A I+P+NFP+ + A+A GN+ + KP+E+ P + +L +AG+P+ V + Sbjct: 689 ACISPWNFPLSIFTGQLSAALAAGNAVLAKPAEQTPLIAFRAVQLMHQAGVPEDVLLFLP 748 Query: 206 GDKVAVDA-LLEHPEVQAVSFVGSTPIAEYIYRKATERA----GTFPLRAQALGGAKNHL 260 G A L+ V V F GST +A I+R +R+ PL A+ G +N + Sbjct: 749 GRGQTTGAQLVADARVSGVVFTGSTQVATIIHRTLAQRSMAERRDIPLIAET--GGQNAM 806 Query: 261 VVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADRLVEALIPRVKALKVKNGM 320 +V A +Q V +I +A+ SAG+RC A+ V +A++ + L + L+ Sbjct: 807 IVDSSALCEQVVRDVIASAFDSAGQRCSALRVLCLQTDIAEKTLNMLHGAFEELQTGRPD 866 Query: 321 EDDAEMGPLVTAAHKAKVEGYI-QSGVDAGAQLLVDGRGLKVPGH-EEGFFIGGTLFDHV 378 + ++GP++ A + + YI Q+G A + + L +P +G F+ + + Sbjct: 867 QLATDVGPVIDAEAQQHLRAYIEQAGRRAK-----NMKQLALPSTGTQGTFVAPCIIE-- 919 Query: 379 TPDMKIYQEEIFGPVLCVVRV--PDFASAVQLINAHEYGNGVSLFTADGNTAHEFSRRIQ 436 M Q E+FGPVL V+R D V+ INA +G + + + T + + Sbjct: 920 IDRMSDLQREVFGPVLHVLRYRRDDLPQLVRDINATGFGLTLGVHSRIDETINYIVDHAR 979 Query: 437 VGMVGINVPI 446 VG + +N I Sbjct: 980 VGNIYVNRNI 989 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 922,078 Number of extensions: 38502 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 116 Number of HSP's successfully gapped: 3 Length of query: 503 Length of database: 1,000,347 Length adjustment: 86 Effective length of query: 417 Effective length of database: 716,977 Effective search space: 298979409 Effective search space used: 298979409 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)