BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4595.p (396 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095304|ref|YP_001100379.1| amidase [Herminiimonas arsenico... 94 1e-20 gi|134096406|ref|YP_001101481.1| putative amidase [Herminiimonas... 86 4e-18 gi|134093455|ref|YP_001098530.1| aspartyl/glutamyl-tRNA amidotra... 79 4e-16 gi|134095844|ref|YP_001100919.1| putative amidase [Herminiimonas... 67 2e-12 >gi|134095304|ref|YP_001100379.1| amidase [Herminiimonas arsenicoxydans] Length = 427 Score = 93.6 bits (231), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 102/371 (27%), Positives = 164/371 (44%), Gaps = 33/371 (8%) Query: 25 GPLAGVRLAVKDIFDVAGYPTGCGNPHMLAMSGIKNSSAPAVQALLQAGAQFVGKVYTDE 84 GPL GV + +KD+ D PT G+ + A V + +AG +GK T E Sbjct: 60 GPLHGVPIGIKDVIDTIDLPTEYGS--TIYAGHRPRREASCVSLVRRAGGVILGKTVTTE 117 Query: 85 LAFSMNGKNAHFGTPRNGGAPDRIPGGSSSGSASAVSNGLAELALGTDTGGSVRVPASHC 144 A GK + P N P PGGSSSGSA+AV++ +A+GT T GS+ PAS+C Sbjct: 118 FALYQPGKTTN---PHN---PAHTPGGSSSGSAAAVADFHVPVAMGTQTSGSIIRPASYC 171 Query: 145 GLIGLRPTHGRVSLAGVMELAGSFDTCGWFAREMGLFSRAGDILLGQDSIE--LPEQPRV 202 G++G +PT +L G LA S DT G+ R + + ++ LG+ +++ +P + Sbjct: 172 GVVGYKPTFNTFALEGTKPLAASLDTLGYLTRTVDDLTLMREVFLGKSAVKSTFNNRPHI 231 Query: 203 MVAADVL---ALLESRVQAVFSQTLDRLAGVIGTPQPVKTATPSFDTLYWAFRHIQGYEA 259 + A ++R VF QT + LA + V PSF L + YE Sbjct: 232 AICRSPFWYEATADTRT--VFEQTAELLANSGFDIEEVDLP-PSFSDLIDVHEKLMAYEI 288 Query: 260 WQAHGQHIRRHDF----QLGPGVAERFTWSATITPQQMAQHSAVRAIFAETFTRLLGRDG 315 + ++R D +LGP + ++ + + AV + F Sbjct: 289 AR---NYVREFDVSVRHELGPKTRQVIEDGWHVSTDEYLRLRAVTRHARDKFAAFSNEFD 345 Query: 316 VLLLPSAPDVAPLLTDSEQSLENYRNQAVRMLCLAGLSGCPQISLPL-MRLDDAPFGLSL 374 +++P+AP AP ++ + + + + L G P I+LP + P G+ Sbjct: 346 AVMVPAAPGDAPDISATGNPIFSR---------VWSLLGTPAIALPAGAGESNLPVGVQF 396 Query: 375 IAPPGSDRSLI 385 +A +D LI Sbjct: 397 VADLDADIRLI 407 >gi|134096406|ref|YP_001101481.1| putative amidase [Herminiimonas arsenicoxydans] Length = 437 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 66/161 (40%), Positives = 88/161 (54%), Gaps = 4/161 (2%) Query: 21 QVSTGPLAGVRLAVKDIFDVAGYPTGCGNPHMLAMSGIKNSSAPAVQALLQAGAQFVGKV 80 Q G L GV + VKD+ D A PT G+P + + A V A AGA +GK Sbjct: 62 QAPGGALHGVPIGVKDLMDTADMPTTYGSP--IYAGHQPQTDAICVAACRNAGAVIMGKT 119 Query: 81 YTDELAFSMNGK--NAHFGTPRNGGAPDRIPGGSSSGSASAVSNGLAELALGTDTGGSVR 138 T E A G N H +G PGGSSSGSA+AV+ G+ +A GT T GS+ Sbjct: 120 VTTEFATFHPGPTCNPHRTHKADGTLQVHTPGGSSSGSAAAVACGMVPVAFGTQTAGSIV 179 Query: 139 VPASHCGLIGLRPTHGRVSLAGVMELAGSFDTCGWFAREMG 179 PA++CG++G +PT+G + LAG+ L+ S DT G AR +G Sbjct: 180 RPAAYCGVVGYKPTYGMLPLAGIKSLSPSLDTVGVLARSVG 220 >gi|134093455|ref|YP_001098530.1| aspartyl/glutamyl-tRNA amidotransferase subunit A [Herminiimonas arsenicoxydans] Length = 485 Score = 79.0 bits (193), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 107/415 (25%), Positives = 170/415 (40%), Gaps = 56/415 (13%) Query: 23 STGPLAGVRLAVKDIFDVAGYPTGCGNPHMLAMSGIKNSSAPAVQALLQAGAQFVGKVYT 82 + G L G+ +A KDIF + + G+ + + + A V+ QAG +GK+ Sbjct: 62 NAGVLTGIPIAHKDIFVTRNWRSTAGSKML--ENYVSPFDATVVENFNQAGMVTLGKLNC 119 Query: 83 DELAFSMNGKNAHFGTPRNGGAPDRIPGGSSSGSASAVSNGLAELALGTDTGGSVRVPAS 142 DE A + +N++FG +N IPGGSS GSA+A++ L + TDTGGS+R PA+ Sbjct: 120 DEFAMGSSNENSYFGAVKNPWDKTAIPGGSSGGSAAAIAARLTPASTATDTGGSIRQPAA 179 Query: 143 HCGLIGLRPTHGRVSLAGVMELAGSFD----------TCGWFAREMGLFSRAGDILLGQD 192 CG+ G++PT+GRVS G++ A S D CG M F + +D Sbjct: 180 LCGVTGIKPTYGRVSRFGMIAFASSLDQGGPIAKTAEDCGLLLNAMVSFDERDSTSVERD 239 Query: 193 SIELPEQ------------PRVMVAADVLALLESRVQAVFSQTLDRLAGVIGTPQP-VKT 239 + PR A + A +E V+A + A ++ P + Sbjct: 240 KEDFTRDLNAPLEGLKIGIPREYFGAGLSADVEQAVRAALGEYEKLGATLVDISLPKTEL 299 Query: 240 ATPS---------------FDTLYWAFRHIQGYEAWQAHGQHIRRHDFQLGPGVAERFTW 284 + P+ FD + + FR + Y R F G V R Sbjct: 300 SIPTYYVIAPAEASSNLSRFDGVRYGFR-AKDYTDLSDMYCKTRAEGF--GEEVKRRILV 356 Query: 285 SATITPQ-----QMAQHSAVRAIFAETFTRLLGRDGVLLLPSAPDVAPLLTD-SEQSLEN 338 A + Q +R + A+ F + V++ P AP VA L D ++ + N Sbjct: 357 GAYVLSHGYYDAYYLQAQKIRRLIAQDFQQAFTLCDVIMGPVAPSVAWDLGDKADDPVAN 416 Query: 339 YRNQAVRMLCLAGLSGCPQISLPL-----MRLDDAPFGLSLIAPPGSDRSLIRYA 388 Y + L+G P +S+P + P GL +I ++ L+ A Sbjct: 417 YLADIFTLST--SLAGLPGMSIPCGFGQGEKNAKRPVGLQIIGNYFAEAKLLNVA 469 >gi|134095844|ref|YP_001100919.1| putative amidase [Herminiimonas arsenicoxydans] Length = 469 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 51/132 (38%), Positives = 71/132 (53%), Gaps = 2/132 (1%) Query: 25 GPLAGVRLAVKDIFDVAGYPTGCGNPHMLAMSGIKNSSAPAVQALLQAGAQFVGKVYTDE 84 GPL G+ ++VKD+ V G G+ M + I AP+V + AGA VG T E Sbjct: 72 GPLHGLPISVKDLIAVGGVRQTFGSRAM--ENNIAQVDAPSVARVKAAGACIVGISTTSE 129 Query: 85 LAFSMNGKNAHFGTPRNGGAPDRIPGGSSSGSASAVSNGLAELALGTDTGGSVRVPASHC 144 G + G RN ++ PGGSS G+A++V+ G+ ALGTD GGS+R P+S Sbjct: 130 FGCKAVGDSPLTGITRNPWNIEKTPGGSSCGAAASVAAGVTPFALGTDGGGSIREPSSFT 189 Query: 145 GLIGLRPTHGRV 156 GL G++ RV Sbjct: 190 GLFGIKAQFARV 201 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.135 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 771,488 Number of extensions: 32246 Number of successful extensions: 81 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 76 Number of HSP's successfully gapped: 4 Length of query: 396 Length of database: 1,000,347 Length adjustment: 84 Effective length of query: 312 Effective length of database: 723,567 Effective search space: 225752904 Effective search space used: 225752904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)