BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4547.p (657 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093460|ref|YP_001098535.1| putative penicillin-binding pro... 944 0.0 gi|134095977|ref|YP_001101052.1| division-specific transpeptidas... 108 6e-25 >gi|134093460|ref|YP_001098535.1| putative penicillin-binding protein 2 (PBP-2) (MrdA) [Herminiimonas arsenicoxydans] Length = 635 Score = 944 bits (2439), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/632 (71%), Positives = 535/632 (84%), Gaps = 1/632 (0%) Query: 1 MTEFKNTAHELRLFRMRLLAASVLVLVCFSLLLARFLWLQVVRHDAYAAQAEENRISVVP 60 MTE KNT EL+LFR+RL A LV + F+ L ARF+WLQV +H+ Y AQAE+NRIS+VP Sbjct: 1 MTELKNTERELQLFRLRLSVAGGLVFILFACLFARFIWLQVYKHNDYVAQAEDNRISIVP 60 Query: 61 IVPNRGLIMDRNGVVLARNYSAYTLEITPSKIKTNIDDLIDELSTIVEIQPRDRRRFRRL 120 +VPNRGLI DRNGV+LARNYSAYTLEIT SKI +++ LIDEL+T+V+IQP+DR+RF++L Sbjct: 61 VVPNRGLIFDRNGVLLARNYSAYTLEITASKINGDLETLIDELATLVDIQPKDRKRFKKL 120 Query: 121 QEESKNFESVPIRTRLTDEEVAKFTAQRYRFPGVEVQARLFRQYPYGKTAAHVIGYIGRI 180 QEES+N S PIR RLTDEEVA+F+AQRYRFPGVE+QARLFRQYP G A+HVIGYIGRI Sbjct: 121 QEESRNLASSPIRVRLTDEEVARFSAQRYRFPGVEIQARLFRQYPLGSVASHVIGYIGRI 180 Query: 181 SQRDAERIADSDDEANYNGTDYIGKEGLEKSYETQLHGTTGYEEVEISASGRAVRSLSRT 240 + R+A+ I +SDD NY G+++IGKEGLEK+YE +LHG TGYEEVE+SA GR +R+LSRT Sbjct: 181 NTREAKEIDESDDATNYRGSEHIGKEGLEKNYEDRLHGRTGYEEVEVSAGGRPIRTLSRT 240 Query: 241 PATPGKNLILSIDIELQKVVEEAFGDQKGALVAINPATGDILAYVSQPSYDPNLFVEGID 300 ATPG NLILSIDIELQKVVE+AFG+++GAL+AI P+TGD+LAYVS P++DPNLFVEGID Sbjct: 241 AATPGDNLILSIDIELQKVVEQAFGNRRGALIAIEPSTGDVLAYVSMPTFDPNLFVEGID 300 Query: 301 QQSWDELNKSPDRPLLNRPLSGLYSPGSTYKPFMALAALELGKRRPQDAIRDPGFFVLGN 360 QQ+WD LN SPD PLLNRPLSG Y PGSTYKPFMALAALELGKR+P DAI DPG+F GN Sbjct: 301 QQNWDALNNSPDHPLLNRPLSGAYPPGSTYKPFMALAALELGKRKPSDAIADPGYFWFGN 360 Query: 361 HRFRDDKEGGHGIVDMYRSIVVSCDTYYYMLANDMGVNAIHDFMKPFGFGQITGIDLEHE 420 H+FRDDKEGGHG+VDMY+SIV SCDTYYY+LAND+GV+AIHDFMKPFGFGQ TGIDL E Sbjct: 361 HKFRDDKEGGHGMVDMYKSIVQSCDTYYYLLANDLGVDAIHDFMKPFGFGQKTGIDLRFE 420 Query: 421 KRGILPSTDWKRTAFRKPEQKRWIAGDTVSLGIGQGYNSFTPLQLAQATAILANNGVVMK 480 + G+LPST WK A++KPEQKRW AG+T+S+GIGQGYN+FTPLQLA ATA LANNGVVMK Sbjct: 421 RTGVLPSTAWKAAAYKKPEQKRWYAGETISIGIGQGYNTFTPLQLAHATATLANNGVVMK 480 Query: 481 PHLVKIIEDGVTHERTETVPKESYRIPLKQENIDFIKRAMVGVVKEGTGAAAFRGAPYES 540 PHLV+ IE+GVT +RT TVPKESYRIPLKQENID IK AMVGV KEGTGA AF A Y S Sbjct: 481 PHLVREIENGVTRQRTLTVPKESYRIPLKQENIDIIKHAMVGVNKEGTGARAFARAEYVS 540 Query: 541 GGKTGTAQVVNIAKNQKYDSKKLSRIFHDNGLYIGFAPADAPRIAIAAVVENGGWGAGVA 600 GGKTGT+QV+ I K +KYD+ K+S HD+ LY FAPAD PRIA+A +VENGG+G A Sbjct: 541 GGKTGTSQVIAIKKGEKYDASKISDRHHDHALYTAFAPADKPRIALALIVENGGFGGAAA 600 Query: 601 APLVRKAMDYFMLGKRPNEPIKSVAPEAVSAD 632 AP+ + AMD+++LGKRP E V P + AD Sbjct: 601 APIAKAAMDFYLLGKRP-EAKAPVKPAVIPAD 631 >gi|134095977|ref|YP_001101052.1| division-specific transpeptidase, penicillin-binding protein 3 [Herminiimonas arsenicoxydans] Length = 471 Score = 108 bits (271), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 133/519 (25%), Positives = 214/519 (41%), Gaps = 100/519 (19%) Query: 103 LSTIVEIQPRDRRRFRRLQEESKNFESVPIRTRLTDEEVAKFTAQRYRFPGVEVQARLFR 162 ++ ++++ RD RR + + F V ++ ++ + V K + G++ + R Sbjct: 1 MAKLLDMSERD---LRRKLDSDRGF--VYLKRQVELDTVDKIL--KLGIAGIDTRKEYKR 53 Query: 163 QYPYGKTAAHVIGYIGRISQRDAERIADSDDEANYNGTDYIGKEGLEKSYETQLHGTTGY 222 YP G+ AH++G+ + +D+ G+EG+E +++ L G TG Sbjct: 54 YYPEGEVMAHIVGF------------TNVEDK---------GQEGMELAFQKTLVGVTGN 92 Query: 223 EEVEISASGRAVRSLS--RTPATPGKNLILSIDIELQKVVEEAFGD-------QKGALVA 273 V GR V + R P GK+L LSID ++Q + A + + G +V Sbjct: 93 RRVIKDRLGRIVEDIESVREPHD-GKDLTLSIDSKIQYIAFSALKEAVELHKAKAGGIVV 151 Query: 274 INPATGDILAYVSQPSYDPNLFVEGIDQQSWDELNKS--PDRPLLNRPLSGLYSPGSTYK 331 ++ TG++LA + P+Y+PN N+S L NR L+ + PGST K Sbjct: 152 LDAKTGEVLALANLPTYNPN--------------NRSVLTGAQLRNRVLTDTFEPGSTLK 197 Query: 332 PFMALAALELGKRRPQDAIRD-PGFFVLGNHRFRDDKEGGHGIVDMYRSIVVSCDTYYYM 390 PF ALE P I+ PG +G D G G++ + + I S + Sbjct: 198 PFTIALALENKHITPNTMIQTAPGKLTIGKATIGDAHAG--GLLSVSQVIEKSSNVGTAK 255 Query: 391 LANDMGVNAIHDFMKPFGFGQITGIDLEHEKRG-ILPSTDWKRTAFRKPEQKRWIAGDTV 449 +A +M + D GFGQ + G + P W R I T+ Sbjct: 256 IALEMEPQEMWDMFTSVGFGQQPKVGFPGAVAGRVRPYKSW-----------RPIEQATM 304 Query: 450 SLGIGQGYNSFTPLQLAQATAILANNGVVMKPHLVKIIEDGVTHERTETVPKESYRIPLK 509 S G G S + LQ+A A I A +G ++ +T +R+ P I K Sbjct: 305 SYGHGI---STSLLQIAHAYMIFARDGEMIP----------LTFQRSSDQPISQRVISEK 351 Query: 510 QENIDFIKRAMVGVVKEGTGAAAFRGAP-YESGGKTGTAQVVNIAKNQKYDSKKLSRIFH 568 + R M+ + TG A P Y GKTGTA Y +K + Sbjct: 352 NAR---LTRGMLEMAAGPTGTAPRAQVPGYRVAGKTGTA----------YKLEKGQYVRK 398 Query: 569 DNGLYIGFAPADAPRIAIAAVVENGG----WGAGVAAPL 603 ++GFAP PRI +A +++ +G VAAP+ Sbjct: 399 YVASFVGFAPVSDPRIIVAVMIDEPAVGRHYGGQVAAPV 437 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.317 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,245,991 Number of extensions: 52705 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 2 Length of query: 657 Length of database: 1,000,347 Length adjustment: 88 Effective length of query: 569 Effective length of database: 710,387 Effective search space: 404210203 Effective search space used: 404210203 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)