BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4484.p (404 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095879|ref|YP_001100954.1| acridine efflux pump protein [H... 87 2e-18 gi|134093794|ref|YP_001098869.1| hypothetical protein HEAR0533 [... 80 2e-16 gi|134095558|ref|YP_001100633.1| acridine efflux pump protein [H... 79 3e-16 gi|134094772|ref|YP_001099847.1| hypothetical protein HEAR1556 [... 79 3e-16 gi|134094823|ref|YP_001099898.1| putative RND divalent metal cat... 70 2e-13 >gi|134095879|ref|YP_001100954.1| acridine efflux pump protein [Herminiimonas arsenicoxydans] Length = 407 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 100/370 (27%), Positives = 169/370 (45%), Gaps = 20/370 (5%) Query: 39 LLAAGAARTVINRVANARALEAGTAERAKVFVRVAPARSSADGQTLALPGTLQGFVQSPI 98 L + GAA ++ VA +A A V V +SSA G T LPG L+ + I Sbjct: 7 LTSLGAAFFILFAVAGCGKSDAPAAAPPPTEVSVVTIKSSALGVTNELPGRLESTRIAEI 66 Query: 99 SARSGGY-LKRWYKDIGAEVKKGDLLAELDTPEIDQQLSQALATRQQAASSMELARSSMA 157 AR G LKR +++ G++VK G L +D + + A A +A +++ A Sbjct: 67 RARVPGVVLKRTFRE-GSDVKAGATLFLIDPAQFKATYNSANAAVARAEATLLQAELKEK 125 Query: 158 RWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVEGFKRIVAPFSGIITRR 217 R++ L +A+S+QE D+ + QA ++LAA+ A E R + + +P SG I R Sbjct: 126 RYKPLVATNAISKQEYDDVTATLKQAEADLAASKAARETARLSLDYATVTSPISGRIGRA 185 Query: 218 NVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSYANLVK------------PGQ-QVTV 264 V G L+ L + Q DP +YVN+ QS +++ G+ QV V Sbjct: 186 LVTEGALVGQNEATPLATVQQIDP--IYVNMTQSSTEILQLQRALADGKLKSAGKGQVKV 243 Query: 265 TQAELRGRKFE--GKVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQVALPLKPSGT 322 T G+++ GK+ + ++D + ++ + N D+ LLPG +V+ + + Sbjct: 244 TLVTEDGQEYAQPGKLLFSDLTVDASSGAITLRAEFPNPDSQLLPGMYVRARVEQAINEN 303 Query: 323 LLVAADTLMFRR-DGSLVAAVDEHNRVRLKKVRLGRNFGLTVEVLEGLNGNEKLILNPSD 381 + + R DG+ V V N V + V G G + + +GL +++I+ Sbjct: 304 AITVPQQAISRTPDGAAVMVVSAENTVVSRPVTTGAASGDSWVISDGLKDGDQVIVEGLQ 363 Query: 382 SLAEGDQVEP 391 + G V+P Sbjct: 364 KIKPGATVKP 373 >gi|134093794|ref|YP_001098869.1| hypothetical protein HEAR0533 [Herminiimonas arsenicoxydans] Length = 500 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 68/281 (24%), Positives = 131/281 (46%), Gaps = 28/281 (9%) Query: 98 ISARSGGYLKRWY-KDIGAEVKKGDLLAELDTPEIDQQLSQALATRQQAASSMELARSSM 156 + +R GY+ + Y + +K+G+ +A + P+ + LA ++ + EL ++ Sbjct: 138 VQSRVTGYIDKLYARSPMQRIKRGEAIASIFVPDWIAPQEEYLALKR--GGNTELMAAAR 195 Query: 157 ARWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVEGFKRIVAPFSGIITR 216 R A+ D + Q +R Q + + +P +G+IT Sbjct: 196 QRMRAMSIPDGLINQ----------------------ADRTGQSQQHFTLTSPVTGVITE 233 Query: 217 RNVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVTQAELRGRKFEG 276 +V G ++ PG ++ ++ + + + VP++ +N+V+ G V T + RK+ G Sbjct: 234 LSVRDGAMVAPGM--TIAKVAGLNKVWLVAEVPEALSNVVRSGMTVEATFSGDANRKYSG 291 Query: 277 KVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQVALPLKPS-GTLLVAADTLMFRRD 335 KV I TR++Q + L N D +L PG ++V L S LLV ++ ++ Sbjct: 292 KVREILPGISTATRTLQARLELDNRDGSLTPGMLMRVRLNADKSVSKLLVPSEAVIANGK 351 Query: 336 GSLVAAVDEHNRVRLKKVRLGRNFGLTVEVLEGLNGNEKLI 376 S+V V E+N ++ V +GR+ G E+L GL+ +K++ Sbjct: 352 QSIVLVVGENNSMQPVVVTIGRDIGSDTEILNGLSDGQKVV 392 >gi|134095558|ref|YP_001100633.1| acridine efflux pump protein [Herminiimonas arsenicoxydans] Length = 403 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 82/310 (26%), Positives = 151/310 (48%), Gaps = 23/310 (7%) Query: 86 LPGTLQGFVQSPISARSGGYLKRWYKDIGAEVKKGDLLAELDTPEIDQQLSQALATRQQA 145 LPG F+ + + + G +K+ GA+VK G L +LD + A A +A Sbjct: 56 LPGRTNAFLVAEVRPQVNGIIKKRLFTEGAQVKAGQPLYQLDDAIYQANYASAKAALARA 115 Query: 146 ASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVEGFKR 205 ++++ A + R L K DAVS+Q+ + +A +Q+ +++AAA A ++ G+ + Sbjct: 116 QATLKSAELNATRTAELVKIDAVSRQDNENAEAALSQSKADVAAAQAALQSNSVTLGYAQ 175 Query: 206 IVAPFSGIITRRNVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVT 265 I +P +G I + +V G L+ +L + Q +P VY+++ QS + L++ +++ Sbjct: 176 ITSPITGRIGKSSVTQGALVTANQAAALATVQQLNP--VYIDLTQSSSELLQLRKELAAG 233 Query: 266 QAELRGR-------------KFEGKVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQ 312 + G + +GK+A ++DP T S + VT+ N DN L+PG +V+ Sbjct: 234 RLTQSGNLPVQIVLEDGSRYQHDGKLAFADVTVDPGTGSFALRVTVPNPDNILMPGMYVR 293 Query: 313 VALPLKPSGT----LLVAADTLMFRRDGSLVA-AVDEHNRVRLKKVRLGRNFGLTVEVLE 367 L +G +LV + G+ A VD+ +V L+ V + R G V + Sbjct: 294 A---LIGNGVRQNAILVPQQGIARDPKGNTSAMVVDKDGKVALRPVSVSRTIGDKWLVED 350 Query: 368 GLNGNEKLIL 377 GL +++I+ Sbjct: 351 GLAAGDRVIV 360 >gi|134094772|ref|YP_001099847.1| hypothetical protein HEAR1556 [Herminiimonas arsenicoxydans] Length = 497 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 67/281 (23%), Positives = 129/281 (45%), Gaps = 28/281 (9%) Query: 98 ISARSGGYLKRWY-KDIGAEVKKGDLLAELDTPEIDQQLSQALATRQQAASSMELARSSM 156 + +R GY+ + Y + +K+G+ +A + P+ + LA ++ + ELA ++ Sbjct: 135 VQSRVTGYIDKLYARSPQQRIKRGEAIASIFAPDWIAPQEEYLALKR--GGNTELASAAR 192 Query: 157 ARWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVEGFKRIVAPFSGIITR 216 R A+ D + Q +R Q + + + +P +G+IT Sbjct: 193 QRMRAMSIPDGLINQ----------------------ADRTGQSQKYFTLTSPVTGVITE 230 Query: 217 RNVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVTQAELRGRKFEG 276 +V G ++ PG ++ ++ + + VP++ +N V+ G V T + RK+ G Sbjct: 231 LSVRDGAMVTPGM--TIAKVAGLSKVWLVAEVPEALSNAVRSGMTVEATFSGDANRKYSG 288 Query: 277 KVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQVALPL-KPSGTLLVAADTLMFRRD 335 KV I TR++Q + L N D +L PG +++ L KP LLV ++ ++ Sbjct: 289 KVREILPGISTATRTLQARLELDNRDGSLTPGMLMRIRLDAEKPVSRLLVPSEAVIANGK 348 Query: 336 GSLVAAVDEHNRVRLKKVRLGRNFGLTVEVLEGLNGNEKLI 376 S+V +N ++ V GR+ G E+L GL+ +K++ Sbjct: 349 QSIVLVAGANNSMQPVVVTTGRDSGNDTEILSGLSEGQKVV 389 >gi|134094823|ref|YP_001099898.1| putative RND divalent metal cation efflux membrane fusion protein (czcBlike) [Herminiimonas arsenicoxydans] Length = 404 Score = 69.7 bits (169), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 79/298 (26%), Positives = 139/298 (46%), Gaps = 21/298 (7%) Query: 83 TLALPGTLQGFVQ---SPISARSGGYLKRWYKDIGAEVKKGDLLAELDTPEIDQQLSQAL 139 TL LPG +Q F + + + R G ++ D+G +VKKG +LA + + + +Q S+ L Sbjct: 90 TLILPGEIQ-FNEDRTAHVVPRLSGVVQSVAADLGQQVKKGQVLAVIASTGLSEQRSELL 148 Query: 140 ATRQQAASSMELARSSMAR----WE---ALRKKDAVSQQELDERRSAFAQASSNLAAADA 192 + +++ M LAR + R WE + + +QQ L E A AS LAA A Sbjct: 149 SAQKR----MALARLTFEREKKLWEEKISAEQDYLQAQQTLRESEIAVQNASQKLAALGA 204 Query: 193 NVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRSLFVLSQTDPLRVYVNVPQSY 252 ++ I APF G++ +++ +G+ + A +F +S + + V Sbjct: 205 GSGSSGAFNRYE-IRAPFDGMVVAKHIALGEAVKEDA--DIFTISDLSSVWAEIAVSAKD 261 Query: 253 ANLVKPGQQVTVTQAELRGRKFEGKVARTAGSIDPVTRSMQVEVTLSNADNALLPGAFVQ 312 N V+ G++V V +A G +A + TR+ VTL+N PG FV Sbjct: 262 LNAVRVGEKVIV-KASAFDSSASGTIAYVGSLLGEQTRTATARVTLANPQGTWRPGLFVS 320 Query: 313 VALPLKPSGT-LLVAADTLMFRRDGSLVAAVDEHNRVRLKKVRLGRNFGLTVEVLEGL 369 + + + + V+A+ L D +V V + ++V GR+ G +VE+++G+ Sbjct: 321 IEIVAGETSVPVSVSAEALHSINDKPVV-FVKVADGFIAQQVTTGRSDGKSVEIVKGM 377 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.316 0.130 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 648,401 Number of extensions: 23978 Number of successful extensions: 116 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 11 Length of query: 404 Length of database: 1,000,347 Length adjustment: 84 Effective length of query: 320 Effective length of database: 723,567 Effective search space: 231541440 Effective search space used: 231541440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)