BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4456.p (487 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrog... 259 2e-70 gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde deh... 172 4e-44 gi|134095327|ref|YP_001100402.1| multifunctional: transcriptiona... 147 8e-37 >gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrogenase I, NADP-dependent [Herminiimonas arsenicoxydans] Length = 492 Score = 259 bits (662), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 157/468 (33%), Positives = 252/468 (53%), Gaps = 11/468 (2%) Query: 19 GRWEPAAQGATVIEPATGQPLTEVGLATPQDVDISVQAAVAAQAEWQAVPPRERADLFRR 78 G W AA + PA G + ++ + +++AA +A W A +ERA + R+ Sbjct: 25 GTWAGAATNFDISNPADGSIIGSAPNMGAEETETAIRAAQSAFPAWAAKTGKERAIIMRQ 84 Query: 79 AGALFQQHFDELARMVARETGGVLFKGEHEVREAIILCHLAAAMPLQAQGQVLPSV-PGR 137 L + D+LA ++ E G L + EV + + G V+ S + Sbjct: 85 WFDLIIANVDDLAILMTAEQGKPLIESRGEVIYGASFIEWFSEEAKRVSGDVMASTWNDK 144 Query: 138 LSLARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDARTPVSGGFMLARVFE 197 +L + P+G+ I+P+NFP+ + R VAPA+AAG +V+KP +TP+S +A + Sbjct: 145 RTLTIKQPIGICAAITPWNFPIAMITRKVAPAVAAGCTIVIKPAEQTPLSA-LAIAELAH 203 Query: 198 EAGLPAGLLHVLPGGAE----AGEALVSAPQVPMIAFTGSPAVGRRIGELAGRHLKKVSL 253 AGLPAG+++V+ AE G+ L ++P V ++FTGS VGR + E +KK++L Sbjct: 204 RAGLPAGVINVITADAENSIVVGKTLCASPIVRHVSFTGSTPVGRILMEQCAPTVKKIAL 263 Query: 254 ELGGANALVILEDADLDVAASNAAWGAWLHQGQICMAANRILVHRSQAPGLLERLVEKAR 313 ELGG ++ +DADLD A + A + + GQ C+ NR VH S +++L A Sbjct: 264 ELGGHAPFIVFDDADLDAAVTGALQSKYRNAGQTCVCTNRFYVHESIYDVFVQKLAAGAA 323 Query: 314 HLPVGDGASGQVALGPLIDQRQLQRVHGLVQDSVAAGARLLAGGTYEGL---FYQPTVLA 370 + VG+G V GPLID + +++V V D+V GA ++ GG L FY+PTVL+ Sbjct: 324 RIKVGNGFEQGVLQGPLIDDQAIRKVEQHVSDAVKKGASVMTGGKKHALGGHFYEPTVLS 383 Query: 371 DVKPGMRVFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGVISRSVGRAMAVAAQLR 430 ++ M + EE FGPVA+++ + ++++AIA AN GLA+ +R + R VA +L Sbjct: 384 NITDDMSIMHEETFGPVAAIVKFKTEDEAIAAANNTDFGLASYFYARDISRVWRVAEKLE 443 Query: 431 QGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWITV 478 GMV +N +++E V PFGG +G G G +EY + K++ + Sbjct: 444 YGMVGINTGLISNE-VAPFGGVKQSGLGRE-GSKYGIDEYLEIKYLCM 489 >gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde dehydrogenase, oxidoreductase protein [Herminiimonas arsenicoxydans] Length = 506 Score = 172 bits (435), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 125/420 (29%), Positives = 201/420 (47%), Gaps = 13/420 (3%) Query: 30 VIEPATGQPLTEVGLATPQDVDISVQAAVAAQAEWQAVPPRERADLFRRAGALFQQHFDE 89 V PATG+ V LA+ DV +V AA AA W + P +RA + + L ++H ++ Sbjct: 28 VFNPATGEISANVVLASDADVQTAVAAAKAAAPAWAEMAPLKRARILFKFKDLIERHHND 87 Query: 90 LARMVARETGGVLFKGEHEVREAIILCHLAAAMPLQAQGQVLPSVPGRL-SLARRAPLGV 148 LA + RE G V + EV + + A +P + Q ++ G + + R PLGV Sbjct: 88 LAAAITREHGKVFSDAKGEVTRGLEVVEFACGVPQLLKTQFSDNIGGGIDNWNLRQPLGV 147 Query: 149 IGVISPFNFPLILTLRTVAPALAAGNAVVVKPDARTPVSGGFMLARVFEEAGLPAGLLHV 208 I+PFNFP ++ + ALA GN ++KP R P S M+A + +AGLP G+ +V Sbjct: 148 TAGITPFNFPFMVPMWMAPVALATGNTFILKPSERDP-SPSLMVAELLHQAGLPDGVFNV 206 Query: 209 LPGGAEAGEALVSAPQVPMIAFTGSPAVGRRIGELAGRHLKKVSLE---LGGA-NALVIL 264 L G A + L+ P + ++F GS + I + L LGGA N LV++ Sbjct: 207 LQGDKRAVDGLLQHPDIKAVSFVGSTPIAEYIYQAGAARKGSYPLRVQALGGAKNHLVVM 266 Query: 265 EDADLDVAASNAAWGAWLHQGQICMAANRILVHRSQAPGLLERLVEKARHLPVGDGASGQ 324 DA+L+ A A+ G+ CMA + + S A L+ L+ + + L V +G Sbjct: 267 PDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVAALIPRVKSLKVKNGMESD 326 Query: 325 VALGPLIDQRQLQRVHGLVQDSVAAGARLLAGGT-------YEGLFYQPTVLADVKPGMR 377 +GP++ ++ G ++ + +GA+LL G G F + +V P M Sbjct: 327 AEMGPVVSAAHKAKIEGYIEAGIKSGAKLLVDGRGLTVDGHSNGFFLGGCLFDNVTPEMT 386 Query: 378 VFDEEIFGPVASVISYDSQEQAIALANQNQGGLAAGVISRSVGRAMAVAAQLRQGMVHVN 437 ++ EEIFGPV V+ A+ L N ++ G + + A + ++ GMV +N Sbjct: 387 IYQEEIFGPVLCVVRVPDFSAALDLINAHEFGNGVALFTSDGNTAREFSRRVEIGMVGIN 446 >gi|134095327|ref|YP_001100402.1| multifunctional: transcriptional repressor of proline utilization; proline dehydrogenase; pyrroline-5-carboxylate dehydrogenase [Herminiimonas arsenicoxydans] Length = 1224 Score = 147 bits (372), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 135/438 (30%), Positives = 192/438 (43%), Gaps = 39/438 (8%) Query: 45 ATPQDVDISVQAAVAAQAEWQAVPPRERADLFRRAGALFQQHFDELARMVARETGGVLFK 104 A DV+ ++ A+A +WQ P ERA + A LFQQH E + RE G L Sbjct: 598 ANADDVERALALAIAGAEKWQKTAPSERAGILLNAAELFQQHAPEFIALSVREAGKTLPN 657 Query: 105 GEHEVREAIILCHLAAAMPLQAQGQVLPSVPGRLSLARRAPLGVIGVISPFNFPLILTLR 164 E+RE I +A AQ + LG++ ISP+NFPL + Sbjct: 658 AIGELRETIDFLRYYSAQITTAQME--------------QALGLVACISPWNFPLSIFTG 703 Query: 165 TVAPALAAGNAVVVKPDARTPVSGGFMLARVFEEAGLPAGLLHVLPG-GAEAGEALVSAP 223 ++ ALAAGNAV+ KP +TP+ F ++ +AG+P +L LPG G G LV+ Sbjct: 704 QLSAALAAGNAVLAKPAEQTPLI-AFRAVQLMHQAGVPEDVLLFLPGRGQTTGAQLVADA 762 Query: 224 QVPMIAFTGSPAVGRRIGE-LAGRHL---KKVSL--ELGGANALVILEDADLDVAASNAA 277 +V + FTGS V I LA R + + + L E GG NA+++ A + + Sbjct: 763 RVSGVVFTGSTQVATIIHRTLAQRSMAERRDIPLIAETGGQNAMIVDSSALCEQVVRDVI 822 Query: 278 WGAWLHQGQICMAANRILVHRSQAPGLLERLVEKARHLPVGDGASGQVALGPLIDQRQLQ 337 A+ GQ C A + + A L L L G +GP+ID Q Sbjct: 823 ASAFDSAGQRCSALRVLCLQTDIAEKTLNMLHGAFEELQTGRPDQLATDVGPVIDAEAQQ 882 Query: 338 RVHGLVQDSVAAGAR--------LLAGGTYEGLFYQPTVLADVKPGMRVFDEEIFGPVAS 389 + ++ AG R L + GT +G F P ++ + M E+FGPV Sbjct: 883 HLRAYIEQ---AGRRAKNMKQLALPSTGT-QGTFVAPCIIEIDR--MSDLQREVFGPVLH 936 Query: 390 VISY--DSQEQAIALANQNQGGLAAGVISRSVGRAMAVAAQLRQGMVHVNDQTVNDEC-V 446 V+ Y D Q + N GL GV SR + R G ++VN + V Sbjct: 937 VLRYRRDDLPQLVRDINATGFGLTLGVHSRIDETINYIVDHARVGNIYVNRNIIGAVVGV 996 Query: 447 NPFGGPGIAGNGTSVGGP 464 PFGG G++G G GGP Sbjct: 997 QPFGGEGLSGTGPKAGGP 1014 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,020,940 Number of extensions: 46185 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 3 Length of query: 487 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 402 Effective length of database: 720,272 Effective search space: 289549344 Effective search space used: 289549344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)