BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4405.p (275 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094877|ref|YP_001099952.1| biotin biosynthesis BioH protei... 54 6e-09 >gi|134094877|ref|YP_001099952.1| biotin biosynthesis BioH protein [Herminiimonas arsenicoxydans] Length = 245 Score = 54.3 bits (129), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 57/258 (22%), Positives = 104/258 (40%), Gaps = 19/258 (7%) Query: 22 PVVFGHGWPLDGDMWEYQMNFLAERGYRVIAYDRRGFGRSSQPWNGYDYDTFADDLAELM 81 P+ HGW + +W LA++ G+ +S Y+ D D + Sbjct: 3 PLRLWHGWGMHPAVWSGLCTELAQQDIATQTPALPGYDQSETV-APYNLDALVDAMLP-- 59 Query: 82 DKLDLQGATLVGFSMGGGDVARYIGRHG----SKRVAKAALLGAVTPIFGQAPDFPQGVP 137 AT + + + G + + H +R+++ L+GA TP F Q D+P G+P Sbjct: 60 -------ATNIPYVLCGWSLGALLALHAARRHPQRISRLILIGA-TPSFVQRADWPHGIP 111 Query: 138 AEVFAGIKEGLQKDRAQFISDFAAVFFGTNRPGKSNAVSPAVLAQTFNIAMLASLKGTLD 197 V + D A + F A+ + +S A + L Q L + + + Sbjct: 112 LAVVDEFIAAVAHDPAAALKRFMALSNQNDVHARSMART---LTQMLTANPLPTAETLIA 168 Query: 198 CVSAFSGTDFREDIKKFNFPTLVIHGDDDQIVPIQTTGKLVAEMVPGAQLKIYGGAPHAL 257 ++ +D R I P L++HG D ++P+ + +A+ +P AQL I A H Sbjct: 169 GLTLLRDSDLRAGIADITQPVLLLHGARDPLMPV-AAAQWLADALPQAQLDILPEAAHTP 227 Query: 258 CATHKDQVNADLLAFLKG 275 + + A + +F + Sbjct: 228 FLSDQQHCCASIASFYRA 245 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.321 0.139 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 583,043 Number of extensions: 25895 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 1 Length of query: 275 Length of database: 1,000,347 Length adjustment: 80 Effective length of query: 195 Effective length of database: 736,747 Effective search space: 143665665 Effective search space used: 143665665 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)