BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4370.p (349 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094820|ref|YP_001099895.1| putative alcool dehydrogenase w... 351 2e-98 gi|134095544|ref|YP_001100619.1| zinc-binding alcohol dehydrogen... 115 4e-27 gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldeh... 75 5e-15 >gi|134094820|ref|YP_001099895.1| putative alcool dehydrogenase with NAD(P)-binding [Herminiimonas arsenicoxydans] Length = 352 Score = 351 bits (901), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 193/353 (54%), Positives = 245/353 (69%), Gaps = 8/353 (2%) Query: 1 MQVHAYGAHAADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVP 60 M + Y HA L RR ++DV IDI YCG+CHSD+H R++W G YP VP Sbjct: 3 MTILGYATHAPADDLGPFRFERRDQRSNDVVIDILYCGVCHSDIHNARNDWGGAVYPMVP 62 Query: 61 GHEIVGRVAAVGAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYC-DNMVGTYNG--K 117 GHEI+GRVA+VG +V++FKVGD VGVGC+VDSCQ C C++ LE YC + TYN + Sbjct: 63 GHEIIGRVASVGVNVTRFKVGDKVGVGCMVDSCQHCAACQKNLEQYCAEGSTFTYNAIDR 122 Query: 118 TDDAPGWTMGGYSQQIVVHERYVLTIRHPEA-DLAAVAPLLCAGITTYSPLRHWGAGPGK 176 D P T GGYS++IVV +++VL + P+ DLA APLLCAGITT+SPLRHW G G Sbjct: 123 HDHMP--TYGGYSERIVVSDKFVLKV--PDGLDLAGAAPLLCAGITTWSPLRHWKVGKGS 178 Query: 177 KVGVVGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRAAAKALGADEVVVSRNAEEMAAHAK 236 KV VVG+GGLGHMG+K+A+A+GA V FT S K A+ LGA VV+S +A +M A Sbjct: 179 KVAVVGLGGLGHMGLKLANALGAEVTLFTRSPGKEQDARRLGAHHVVLSTDAAQMHAING 238 Query: 237 SFDFILNTVAAPHDLDAFLSLLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGG 296 FD I++TV HD++ ++ L +GT+ LVG P N +IL R+S+AGSLIGG Sbjct: 239 RFDLIIDTVPYIHDINPYIPTLSIDGTLVLVGYLGGLEPMLNSAPMILGRKSVAGSLIGG 298 Query: 297 IPETQEMLDFCAQHGIVADIELIRAEEINSAYERMLKGDIKYRFVIDNASLAA 349 I ETQEMLDFC QHGI +DIELI+ ++IN AY+RMLK D+KYRFVID ASL+A Sbjct: 299 IAETQEMLDFCGQHGITSDIELIKMQDINVAYQRMLKSDVKYRFVIDMASLSA 351 >gi|134095544|ref|YP_001100619.1| zinc-binding alcohol dehydrogenase [Herminiimonas arsenicoxydans] Length = 277 Score = 115 bits (287), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 73/221 (33%), Positives = 110/221 (49%), Gaps = 11/221 (4%) Query: 10 AADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVPGHEIVGRVA 69 AA + L+ + + GA+D+ I + CG+C +DLH + +E +P +PGHEIVG + Sbjct: 8 AAGEALQLRSVEKPVAGANDLLIRVHTCGVCRTDLHLLDAELPNAVFPIIPGHEIVGTIV 67 Query: 70 AVGAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYCDNMVGTYNGKTDDAPGWTMGGY 129 A G+ V F++GD GV + +C C C G EN CD+ ++ G T + GGY Sbjct: 68 ATGSAVQSFRIGDRAGVPWLGHTCGQCSYCVAGQENLCDH--ASFTGCTIN------GGY 119 Query: 130 SQQIVVHERYVLTIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHM 189 ++ + +Y + ++L A APLLCAG+ Y LR A K++GV G G + Sbjct: 120 AEYVAADSQYCFPMPGMYSNLEA-APLLCAGLIGYRALRM--AAGAKRIGVYGFGAAAQI 176 Query: 190 GIKIAHAMGAHVVAFTTSDSKRAAAKALGADEVVVSRNAEE 230 +IA G V FT + A A V + EE Sbjct: 177 ITQIACHQGRTVYGFTRPGDQAAQQLAYDCGVVWAGSSDEE 217 >gi|134095231|ref|YP_001100306.1| glutathione-dependent formaldehyde dehydrogenase [Herminiimonas arsenicoxydans] Length = 369 Score = 75.1 bits (183), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 98/366 (26%), Positives = 147/366 (40%), Gaps = 38/366 (10%) Query: 1 MQVHAYGAHAADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVP 60 M+ A A KPLE + I +V I I G+CH+D + + +P V Sbjct: 1 MKSRAAVAFGPGKPLEIVEIDVEPPRKGEVLIKITNTGVCHTDAFTLSGDDPEGLFPVVL 60 Query: 61 GHEIVGRVAAVGAHVSKFKVGDLVGVGCIVDSCQSCPDCEEGLENYCDNMVGTYNGKTDD 120 GHE G V VG V+ K GD V + C C C+ G N C + T GK Sbjct: 61 GHEGAGIVVEVGEGVTSVKPGDHV-IPLYTAECGECLFCKSGKTNLCVAVRAT-QGKGLM 118 Query: 121 APGWTMGGYSQQIVVHERYVLTIRH----PEADLAAVAP--------LLCAGITTYSPLR 168 G T Y+ Q + H T E LA + P LL G+TT Sbjct: 119 PDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPDANPEHVCLLGCGVTTGIGAV 178 Query: 169 HWGAG--PGKKVGVVGIGGLGHMGIKIA-HAMGAHVVAFTTSDSKRAAAKALGADEVVVS 225 H A PG V + G+GG+G I+ A A ++A T+ +K A+ GA E + Sbjct: 179 HNTAKVQPGDSVAIFGLGGIGLAAIQGARQAKAGRIIAIDTNPAKFELARTFGATECLNP 238 Query: 226 RNAE--------EMAAHA--KSFDFILNTVAAPHDLDAFLSLLKREGTMTLVGAPATPHP 275 ++ + EM +F+ I N L+A + + + GA Sbjct: 239 KDFDKPIHEVLIEMTGWGVDHTFECIGNVNVMRSALEAAHRGWGQSIVIGVAGAGKEIST 298 Query: 276 SPNVFNLILKRRSLAGSLIGGIPETQEMLDFCAQHGIVADIEL-------IRAEEINSAY 328 P F L+ R+ GS GG+ + + L + + +I+L + +EIN A+ Sbjct: 299 RP--FQLVTG-RTWKGSAFGGV-KGRTQLPGMVEDAMKGEIDLAPFVTHTMGLDEINHAF 354 Query: 329 ERMLKG 334 + M +G Sbjct: 355 DLMHEG 360 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.321 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 655,321 Number of extensions: 25888 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 83 Number of HSP's successfully gapped: 5 Length of query: 349 Length of database: 1,000,347 Length adjustment: 83 Effective length of query: 266 Effective length of database: 726,862 Effective search space: 193345292 Effective search space used: 193345292 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)