BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4337.p (404 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134094823|ref|YP_001099898.1| putative RND divalent metal cat... 459 e-131 gi|134093785|ref|YP_001098860.1| cobalt-zinc-cadmium resistance ... 372 e-104 gi|134094811|ref|YP_001099886.1| cobalt-zinc-cadmium resistance ... 362 e-101 gi|134096397|ref|YP_001101472.1| putative heavy metal efflux sys... 77 1e-15 >gi|134094823|ref|YP_001099898.1| putative RND divalent metal cation efflux membrane fusion protein (czcBlike) [Herminiimonas arsenicoxydans] Length = 404 Score = 459 bits (1182), Expect = e-131, Method: Compositional matrix adjust. Identities = 237/408 (58%), Positives = 309/408 (75%), Gaps = 13/408 (3%) Query: 1 MSQKQTFTIATIIVMAALVAGITLMKGNGSQGENAKPSAHQEEGGHQDNEHPGEDQD-SD 59 +++KQ + II ++AG+ L G + +A +E GH D+ GE + + Sbjct: 6 LNKKQGIAVMAIITAGLVLAGLILFSG--------QHAAVGDEHGH-DSRAKGERHEHGE 56 Query: 60 HKEE--GKIALDQEQMAAAAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVE 117 KEE KI L Q+ AA + ++ GA I ++L LPGEI+FNEDRTAHVVP+++G+V+ Sbjct: 57 SKEERRDKIVLSDAQIKAAGVTMQITGAANIRTTLILPGEIQFNEDRTAHVVPRLSGVVQ 116 Query: 118 SVSATLGQKVKKGQVLAVINSSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQ 177 SV+A LGQ+VKKGQVLAVI S+ +SE RSELL+A++R++LAR ++REKKLWE+KISAEQ Sbjct: 117 SVAADLGQQVKKGQVLAVIASTGLSEQRSELLSAQKRMALARLTFEREKKLWEEKISAEQ 176 Query: 178 DYLQAQSALREAEVAVRNAQQKLRAIGA-SGSSGSLNRYEIRAPFDGTVVEKHLGLGEAV 236 DYLQAQ LRE+E+AV+NA QKL A+GA SGSSG+ NRYEIRAPFDG VV KH+ LGEAV Sbjct: 177 DYLQAQQTLRESEIAVQNASQKLAALGAGSGSSGAFNRYEIRAPFDGMVVAKHIALGEAV 236 Query: 237 REDANAFLISDLSSVWAEIIVSPKDMQVVRVGEKVVVQATALSASAQGNISYVGSLIGEQ 296 +EDA+ F ISDLSSVWAEI VS KD+ VRVGEKV+V+A+A +SA G I+YVGSL+GEQ Sbjct: 237 KEDADIFTISDLSSVWAEIAVSAKDLNAVRVGEKVIVKASAFDSSASGTIAYVGSLLGEQ 296 Query: 297 SRTAKAHVVLPNTGEIWRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRVPGGF 356 +RTA A V L N WRPGLFV++E+V+ E VPV+V+A+A+ +++ K VVFV+V GF Sbjct: 297 TRTATARVTLANPQGTWRPGLFVSIEIVAGETSVPVSVSAEALHSINDKPVVFVKVADGF 356 Query: 357 AAQEVTLGRASGKFVEIKSGIDAGTEYAATGSFAIKAELGKGSAEHSH 404 AQ+VT GR+ GK VEI G+ AG+EYA+TGSF IK+ELGK SAEH+H Sbjct: 357 IAQQVTTGRSDGKSVEIVKGMQAGSEYASTGSFVIKSELGKASAEHAH 404 >gi|134093785|ref|YP_001098860.1| cobalt-zinc-cadmium resistance protein CzcB [Herminiimonas arsenicoxydans] Length = 526 Score = 372 bits (956), Expect = e-104, Method: Compositional matrix adjust. Identities = 180/344 (52%), Positives = 255/344 (74%) Query: 61 KEEGKIALDQEQMAAAAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVS 120 +EEG++ + EQ+ +++ TAG +I ++LQL GEIR NEDRT HVVP++AGIV+SV Sbjct: 183 QEEGRVNMTDEQLKQNGVKIMTAGPARIKTNLQLIGEIRLNEDRTVHVVPRLAGIVDSVR 242 Query: 121 ATLGQKVKKGQVLAVINSSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDYL 180 A G +VKKGQVLAVI+S ++S+ RSELL A++RLSLART Y+REK LWE+KISAEQDYL Sbjct: 243 ANAGDQVKKGQVLAVISSQSLSDQRSELLAAQKRLSLARTTYEREKSLWEEKISAEQDYL 302 Query: 181 QAQSALREAEVAVRNAQQKLRAIGASGSSGSLNRYEIRAPFDGTVVEKHLGLGEAVREDA 240 QA++A++EA++ ++AQQKL A+GAS SS +L +YEIR+P DG + +K++ G +EDA Sbjct: 303 QARAAMQEAQIMAQSAQQKLAALGASPSSSNLTQYEIRSPIDGVITDKNISTGSVTKEDA 362 Query: 241 NAFLISDLSSVWAEIIVSPKDMQVVRVGEKVVVQATALSASAQGNISYVGSLIGEQSRTA 300 N F++SDLS+VW ++ V +D+ ++VG+K V+++A G +SY+G+L+GEQ+RTA Sbjct: 363 NIFVVSDLSTVWVDLTVRAEDIGALKVGQKGTVKSSAFDGQDVGAVSYIGALVGEQTRTA 422 Query: 301 KAHVVLPNTGEIWRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRVPGGFAAQE 360 KA +VL N +WRPGL VNV +V+ E VPVA++ D+IQT+ + VVF R A+ Sbjct: 423 KARIVLANPKGLWRPGLPVNVNLVAGEVDVPVAISVDSIQTLGDREVVFGRYGNLLEARP 482 Query: 361 VTLGRASGKFVEIKSGIDAGTEYAATGSFAIKAELGKGSAEHSH 404 + LGR+ GK +E+ SG+ +YA T SF IKAE+GK SA H H Sbjct: 483 LELGRSDGKLIEVLSGLIVDEKYAGTNSFLIKAEIGKSSAGHDH 526 >gi|134094811|ref|YP_001099886.1| cobalt-zinc-cadmium resistance protein CzcB [Herminiimonas arsenicoxydans] Length = 524 Score = 362 bits (930), Expect = e-101, Method: Compositional matrix adjust. Identities = 178/344 (51%), Positives = 249/344 (72%) Query: 61 KEEGKIALDQEQMAAAAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVS 120 +EEG++++ EQ+ +++ TAG +I +SLQL GEIR NED T HVVP++AGIVESV Sbjct: 181 QEEGRVSMTDEQLKLNGVQIMTAGPARIKTSLQLIGEIRLNEDSTVHVVPRLAGIVESVR 240 Query: 121 ATLGQKVKKGQVLAVINSSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDYL 180 A G +VKKGQVLAVI+S ++S+ RSELL A++RLSLART Y+REK LWE+KISAEQDYL Sbjct: 241 ANAGDQVKKGQVLAVISSQSLSDQRSELLAAQKRLSLARTTYEREKTLWEEKISAEQDYL 300 Query: 181 QAQSALREAEVAVRNAQQKLRAIGASGSSGSLNRYEIRAPFDGTVVEKHLGLGEAVREDA 240 QA++ ++EA++ ++AQQKL A+GAS SS +L +YEIR+P +G + +K + G +ED Sbjct: 301 QARAVMQEAQIMAQSAQQKLAALGASSSSSNLTQYEIRSPINGVITDKRISTGTVAKEDD 360 Query: 241 NAFLISDLSSVWAEIIVSPKDMQVVRVGEKVVVQATALSASAQGNISYVGSLIGEQSRTA 300 N F+ISDLS+VW ++ V +D+ ++ G+K V++ A A G +SY+G+L+GEQ+RTA Sbjct: 361 NIFVISDLSTVWVDLTVRAEDIGALKTGQKGTVKSNAFDAQDVGTVSYIGALVGEQTRTA 420 Query: 301 KAHVVLPNTGEIWRPGLFVNVEVVSDEAQVPVAVAADAIQTVDGKNVVFVRVPGGFAAQE 360 KA +VL N IWRPGL VNV +V+ E VPV ++ D++Q + ++VVF R A+ Sbjct: 421 KARIVLANPKGIWRPGLPVNVNLVAGEVDVPVTISTDSVQKMGDRDVVFGRYGNLLEARP 480 Query: 361 VTLGRASGKFVEIKSGIDAGTEYAATGSFAIKAELGKGSAEHSH 404 + LGR GK VE+ G+ A +YA T SF IKAE+GK SA H H Sbjct: 481 IDLGRTDGKLVEVLGGLLADEKYAGTNSFLIKAEIGKSSASHDH 524 >gi|134096397|ref|YP_001101472.1| putative heavy metal efflux system protein [Herminiimonas arsenicoxydans] Length = 470 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 91/347 (26%), Positives = 146/347 (42%), Gaps = 29/347 (8%) Query: 61 KEEGKIALDQEQMAAAAIELKTAGAMQITSSLQLPGEIRFNEDRTAHVVPQVAGIVESVS 120 K + +D + ++++T + ++ I +E RT V +V G + Sbjct: 90 KHSDLVHVDMQTQHRMGVQMQTVAVNDMHRAINTFATISPDESRTVSVNSKVEGWIRRWH 149 Query: 121 AT-LGQKVKKGQVLAVINSSAISELRSELLNAEQRLSLARTAYQREKKLWEQKISAEQDY 179 +GQ+V+KGQVL I S + + + E ++ R R A Q + S + D Sbjct: 150 IQGVGQQVRKGQVLYEIYSPELQQRQREYVDLLTR----RDAMQEK--------SRDMDM 197 Query: 180 LQAQSALREAEVAVRNAQQKLRAIGASGSSGSLNRYE----------IRAPFDGTVVEKH 229 L +A+ +A + + R + A L E IRA G V Sbjct: 198 LGPNAAMM-GSLAKERFRTRDRLLAADIPPEELELLEKGRRVPEVMPIRATQSGIVTAIS 256 Query: 230 LGLGEAVREDANAFLISDLSSVWAEIIVSPKDMQVVRVGEKVVVQATALSASAQ--GNIS 287 G + + SD S VWAE+ + P + +R G+++V+ + L + Q I+ Sbjct: 257 AREGSYINPMQTLMVYSDNSRVWAEVTLFPDQISWLRNGDEIVL-TSGLDNTVQHTARIN 315 Query: 288 YVGSLIGEQSRTAKAHVVLPNTGEIWRPGLFVNVEVVSDEAQVPVAVAADA-IQTVDGKN 346 I SRTAK + L GE + PG + + S A V+V DA I+T G+ Sbjct: 316 LATLQIDPASRTAKLRLPLSKAGEAFLPGAYAKATIKS-AAHRAVSVPRDAVIRTGHGEF 374 Query: 347 VVFVRVPGGFAAQEVTLGRASGKFVEIKSGIDAGTEYAATGSFAIKA 393 VV G F + V +G + VEI+SG+ AG A G F + A Sbjct: 375 VVVSEGDGHFRSAAVAIGLEDERMVEIRSGLKAGDRIAVNGQFLLDA 421 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.311 0.127 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 701,073 Number of extensions: 27548 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 8 Length of query: 404 Length of database: 1,000,347 Length adjustment: 84 Effective length of query: 320 Effective length of database: 723,567 Effective search space: 231541440 Effective search space used: 231541440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 130 (54.7 bits)