BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4326.p (483 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrog... 461 e-131 gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde deh... 191 6e-50 gi|134095327|ref|YP_001100402.1| multifunctional: transcriptiona... 159 2e-40 >gi|134095838|ref|YP_001100913.1| succinate-semialdehyde dehydrogenase I, NADP-dependent [Herminiimonas arsenicoxydans] Length = 492 Score = 461 bits (1186), Expect = e-131, Method: Compositional matrix adjust. Identities = 223/469 (47%), Positives = 316/469 (67%), Gaps = 4/469 (0%) Query: 14 YIGGQWREASDGARFDVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQRS 73 YI G W A FD+ +PA ++ + + A+ AA A PAWAA + ++R+ Sbjct: 22 YIDGTW--AGAATNFDISNPADGSIIGSAPNMGAEETETAIRAAQSAFPAWAAKTGKERA 79 Query: 74 DILRKAFDLLIERKEAFARLIVLESGKALAEARGEVAYAAEFLRWYSEEAVRIQGEVSVA 133 I+R+ FDL+I + A L+ E GK L E+RGEV Y A F+ W+SEEA R+ G+V + Sbjct: 80 IIMRQWFDLIIANVDDLAILMTAEQGKPLIESRGEVIYGASFIEWFSEEAKRVSGDVMAS 139 Query: 134 PGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTALAMA 193 R L ++QPIG+ +TPWNFP AM TRK+ PA+AAGCT+V+KPA +TPL+ALA+A Sbjct: 140 TWNDKRTLTIKQPIGICAAITPWNFPIAMITRKVAPAVAAGCTIVIKPAEQTPLSALAIA 199 Query: 194 EVFEEAGVPAGVINVVPSRKSGEIV--KAMLEDPRVRKLSFTGSTEIGRVLLGQAAQNVV 251 E+ AG+PAGVINV+ + IV K + P VR +SFTGST +GR+L+ Q A V Sbjct: 200 ELAHRAGLPAGVINVITADAENSIVVGKTLCASPIVRHVSFTGSTPVGRILMEQCAPTVK 259 Query: 252 KCSMELGGNAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRRFA 311 K ++ELGG+APFIV DADL+ AV A+ +K RN G++C NRFYV + + F ++ A Sbjct: 260 KIALELGGHAPFIVFDDADLDAAVTGALQSKYRNAGQTCVCTNRFYVHESIYDVFVQKLA 319 Query: 312 QAMAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGKIREGKGYFYEP 371 A +K+G+G E+G+ GPL++ ++KV Q V++AV++GA V+TGGK G+FYEP Sbjct: 320 AGAARIKVGNGFEQGVLQGPLIDDQAIRKVEQHVSDAVKKGASVMTGGKKHALGGHFYEP 379 Query: 372 TLLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLGKALQVA 431 T+L+++ D +I+H E FGPVA I F + DE I AN+T+FGL S+ +++D+ + +VA Sbjct: 380 TVLSNITDDMSIMHEETFGPVAAIVKFKTEDEAIAAANNTDFGLASYFYARDISRVWRVA 439 Query: 432 ERIEAGMVGINRGVVSDPAAPFGGWKQSGVGREGGHDGLLEYLESKYIC 480 E++E GMVGIN G++S+ APFGG KQSG+GREG G+ EYLE KY+C Sbjct: 440 EKLEYGMVGINTGLISNEVAPFGGVKQSGLGREGSKYGIDEYLEIKYLC 488 >gi|134096568|ref|YP_001101643.1| methylmalonate-semialdehyde dehydrogenase, oxidoreductase protein [Herminiimonas arsenicoxydans] Length = 506 Score = 191 bits (486), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 153/481 (31%), Positives = 231/481 (48%), Gaps = 13/481 (2%) Query: 14 YIGGQWREASDGARFDVLDPATEELLTEVASGSIADALDAVGAAYQAGPAWAATSPRQRS 73 YI G G + V +PAT E+ V S AD AV AA A PAWA +P +R+ Sbjct: 12 YINGNLTNTVGGGQQPVFNPATGEISANVVLASDADVQTAVAAAKAAAPAWAEMAPLKRA 71 Query: 74 DILRKAFDLLIERKEAFARLIVLESGKALAEARGEVAYAAEFLRWYSEEAVRIQGEVSVA 133 IL K DL+ A I E GK ++A+GEV E + + ++ + S Sbjct: 72 RILFKFKDLIERHHNDLAAAITREHGKVFSDAKGEVTRGLEVVEFACGVPQLLKTQFSDN 131 Query: 134 PGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIGPALAAGCTVVLKPASETPLTALAMA 193 GG +RQP+GV +TP+NFP + ALA G T +LKP+ P +L +A Sbjct: 132 IGGGIDNWNLRQPLGVTAGITPFNFPFMVPMWMAPVALATGNTFILKPSERDPSPSLMVA 191 Query: 194 EVFEEAGVPAGVINVVPSRKSGEIVKAMLEDPRVRKLSFTGSTEIGRVLL--GQAAQNVV 251 E+ +AG+P GV NV+ K V +L+ P ++ +SF GST I + G A + Sbjct: 192 ELLHQAGLPDGVFNVLQGDKRA--VDGLLQHPDIKAVSFVGSTPIAEYIYQAGAARKGSY 249 Query: 252 KCSME-LGG-NAPFIVLADADLEVAVASAMVAKLRNGGESCTAANRFYVERPVAEEFSRR 309 ++ LGG +V+ DA+LE AV + + A + GE C A + VA++ Sbjct: 250 PLRVQALGGAKNHLVVMPDANLEQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLVAA 309 Query: 310 FAQAMAAVKIGSGLEEGIQLGPLVNSSTLQKVHQLVTEAVEQGAKVLTGGK----IREGK 365 + ++K+ +G+E ++GP+V+++ K+ + ++ GAK+L G+ Sbjct: 310 LIPRVKSLKVKNGMESDAEMGPVVSAAHKAKIEGYIEAGIKSGAKLLVDGRGLTVDGHSN 369 Query: 366 GYFYEPTLLTDVRADATILHREIFGPVAPICIFDSIDEVIRLANDTEFGLVSFVHSKDLG 425 G+F L +V + TI EIFGPV + + L N EFG + + D Sbjct: 370 GFFLGGCLFDNVTPEMTIYQEEIFGPVLCVVRVPDFSAALDLINAHEFGNGVALFTSDGN 429 Query: 426 KALQVAERIEAGMVGINRGV-VSDPAAPFGGWKQSGVG--REGGHDGLLEYLESKYICAS 482 A + + R+E GMVGIN + V FGGWK+S G G +G+ Y K I Sbjct: 430 TAREFSRRVEIGMVGINVPIPVPMAWHSFGGWKRSMFGDTHAYGEEGIKFYTRYKSIMQR 489 Query: 483 W 483 W Sbjct: 490 W 490 >gi|134095327|ref|YP_001100402.1| multifunctional: transcriptional repressor of proline utilization; proline dehydrogenase; pyrroline-5-carboxylate dehydrogenase [Herminiimonas arsenicoxydans] Length = 1224 Score = 159 bits (403), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 122/431 (28%), Positives = 197/431 (45%), Gaps = 33/431 (7%) Query: 49 DALDAVGAAYQAGPAWAATSPRQRSDILRKAFDLLIERKEAFARLIVLESGKALAEARGE 108 D A+ A W T+P +R+ IL A +L + F L V E+GK L A GE Sbjct: 602 DVERALALAIAGAEKWQKTAPSERAGILLNAAELFQQHAPEFIALSVREAGKTLPNAIGE 661 Query: 109 VAYAAEFLRWYSEEAVRIQGEVSVAPGGANRILVVRQPIGVAVFVTPWNFPAAMATRKIG 168 + +FLR+YS + Q E Q +G+ ++PWNFP ++ T ++ Sbjct: 662 LRETIDFLRYYSAQITTAQME---------------QALGLVACISPWNFPLSIFTGQLS 706 Query: 169 PALAAGCTVVLKPASETPLTALAMAEVFEEAGVPAGVINVVPSRKSGEIVKAML-EDPRV 227 ALAAG V+ KPA +TPL A ++ +AGVP V+ +P R G+ A L D RV Sbjct: 707 AALAAGNAVLAKPAEQTPLIAFRAVQLMHQAGVPEDVLLFLPGR--GQTTGAQLVADARV 764 Query: 228 RKLSFTGSTEIGRVLLGQAAQNV------VKCSMELGGNAPFIVLADADLEVAVASAMVA 281 + FTGST++ ++ AQ + E GG IV + A E V + + Sbjct: 765 SGVVFTGSTQVATIIHRTLAQRSMAERRDIPLIAETGGQNAMIVDSSALCEQVVRDVIAS 824 Query: 282 KLRNGGESCTAANRFYVERPVAEEFSRRFAQAMAAVKIGSGLEEGIQLGPLVNSSTLQKV 341 + G+ C+A ++ +AE+ A ++ G + +GP++++ Q + Sbjct: 825 AFDSAGQRCSALRVLCLQTDIAEKTLNMLHGAFEELQTGRPDQLATDVGPVIDAEAQQHL 884 Query: 342 HQLVTEAVEQGAKVLTGGKIREG-KGYFYEPTLL-TDVRADATILHREIFGPVAPICIF- 398 + +A + + G +G F P ++ D +D L RE+FGPV + + Sbjct: 885 RAYIEQAGRRAKNMKQLALPSTGTQGTFVAPCIIEIDRMSD---LQREVFGPVLHVLRYR 941 Query: 399 -DSIDEVIRLANDTEFGLVSFVHSKDLGKALQVAERIEAGMVGINRGVVSDPAA--PFGG 455 D + +++R N T FGL VHS+ + + G + +NR ++ PFGG Sbjct: 942 RDDLPQLVRDINATGFGLTLGVHSRIDETINYIVDHARVGNIYVNRNIIGAVVGVQPFGG 1001 Query: 456 WKQSGVGREGG 466 SG G + G Sbjct: 1002 EGLSGTGPKAG 1012 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 873,350 Number of extensions: 35269 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 3 Length of query: 483 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 398 Effective length of database: 720,272 Effective search space: 286668256 Effective search space used: 286668256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)