BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4313.p (308 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095638|ref|YP_001100713.1| high-affinity branched-chain am... 89 3e-19 gi|134093664|ref|YP_001098739.1| putative ABC transporter permea... 77 2e-15 gi|134095848|ref|YP_001100923.1| high-affinity branched-chain am... 69 2e-13 gi|134096409|ref|YP_001101484.1| putative high-affinity branched... 54 8e-09 >gi|134095638|ref|YP_001100713.1| high-affinity branched-chain amino acid ABC transporter membrane protein [Herminiimonas arsenicoxydans] Length = 404 Score = 89.0 bits (219), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 77/280 (27%), Positives = 126/280 (45%), Gaps = 58/280 (20%) Query: 5 VIALAWNLLAGFGGLIAIGQHAFIGVGAYSLFFLS------------------------- 39 ++AL N++ GF GL+ +G AF +GAY L+ Sbjct: 51 MLALGLNIVVGFAGLLDLGYIAFYAIGAYLTALLASPQFAVVLESFVNQYPPIGAALMWV 110 Query: 40 -------NSLDCAPWLLLPLATLSALAFALLSAWPMFRLSGPYFAVGTWVLAEMLRIAAQ 92 N + + W+++PLA L A F L P +L G Y A+ T E++RI Sbjct: 111 FGSEIAQNGIHLSLWVIVPLAVLVAGFFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMN 170 Query: 93 N----TDVLGAGSGLSL-------------DE-------MNNIDRWTRNAGSYWAALALC 128 N ++ G+++ DE + N + NA Y+ L LC Sbjct: 171 NLNGPVNITNGPQGINMIDPIRIFGVSLNGDEGSASTVMLGNYAMPSVNA-YYFLFLVLC 229 Query: 129 ISAVVVARMTLRGRIGLALMSVRDSEVAATACGVSVRKAKLWIWLIAAAMTGTAGALAYI 188 I+ + ++ R+G A +++R+ E+AA A G++ R KL + + A+ G AGA+ Sbjct: 230 IAIIFISVRLQNSRLGRAFVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFAS 289 Query: 189 NTLQVTPDASFSLNWTAAAIFIAVLGGIGTLEGPFLGTLL 228 V+P+ SFSL + A + + VLGG+G + G LG +L Sbjct: 290 FQGFVSPE-SFSLTESIAVLAMVVLGGMGHIPGVVLGGIL 328 >gi|134093664|ref|YP_001098739.1| putative ABC transporter permease [Herminiimonas arsenicoxydans] Length = 343 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 71/276 (25%), Positives = 131/276 (47%), Gaps = 15/276 (5%) Query: 5 VIALAWNLLAGFGGLIAIGQHAFIGVGAYSLFFLSNSLDCAPW-LLLPLATLSALAFALL 63 VI L +NLL G+GGL++ G FI +GAY+ L + + L+L A +++ ++ Sbjct: 63 VILLGFNLLFGYGGLLSFGHALFIAIGAYAAAALVSKTGMVSFELMLLTAIVASTIISIP 122 Query: 64 SAWPMFRLSGPYFAVGTWVLAEMLR-IAAQNTDVLGAGSGLSLD-------EMNNIDRWT 115 AW R +G +F + T + + + G SG+ + E++ D+ Sbjct: 123 IAWLASRFTGIFFGMLTLSFGMLFHSFLNKFYYITGGDSGMRVPRPTLFGMELDQFDKTA 182 Query: 116 RNAGS-YWAALALCISAVVVARMTLRGRIGLALMSVRDSEVAATACGVSVRKAKLWIWLI 174 G Y+ L + + +V +R G+ LM+ RD+ V A GV +R + ++I Sbjct: 183 FLGGPFYYYCLVMILLLTLVMWRIVRSPYGIHLMASRDNAVKALYLGVKIRSVRAIAFVI 242 Query: 175 AAAMTGTAGALAYINTLQVTPDASFSLNWTAAA--IFIAVLGGIGTLEGPFLGTLLYFFL 232 +A G + +NT P+ ++ WT + +F+AVLGG GP LG ++ L Sbjct: 243 SAVYASIGGVILGVNTGLADPELAY---WTHSGHLVFMAVLGGYQEFLGPVLGAFVFILL 299 Query: 233 RESFSAYGAWYFIGLGSLAIFAMLFSPGGAWAPIKR 268 ++ S+ ++ LG++ ++ PGG +++ Sbjct: 300 QDELSSLTQYWRFFLGAILAILVVVLPGGIMGAVRK 335 >gi|134095848|ref|YP_001100923.1| high-affinity branched-chain amino acid ABC transporter ATP-binding protein [Herminiimonas arsenicoxydans] Length = 608 Score = 69.3 bits (168), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 69/259 (26%), Positives = 120/259 (46%), Gaps = 3/259 (1%) Query: 5 VIALAWNLLAGFGGLIAIGQHAFIGVGAYSLFFLSNSLDCAPWLLLPLATLSALAFALLS 64 ++AL N+L G GL+++GQ +GAY+ LS + A + + AF +L Sbjct: 46 LVALGLNILTGLTGLVSLGQAGLFAIGAYTSAVLSTKFGFGLGTCIVAAMILSAAFGVLL 105 Query: 65 AWPMFRLSGPYFAVGTWVLAEMLR-IAAQNTDVLGAGSGL-SLDEMNNIDRWTRNAGSYW 122 ++P R+ G Y V T ++ +A + + + G +G+ S+ + ++ Sbjct: 106 SYPTVRVRGVYLTVITIAFGIIVENVAIEWSSLTGGPNGISSIPTPTMFGLPLEDTKFFY 165 Query: 123 AALALCISAVVVARMTLRGRIGLALMSVRDSEVAATACGVSVRKAKLWIWLIAAAMTGTA 182 L + A ++ ++ R G A+ +V SE AA G++ ++ ++IAAA G A Sbjct: 166 VLAILFLLAFLLHYNLIKSRFGRAMRAVSQSETAARTVGINPIVMRMMSFVIAAAFAGAA 225 Query: 183 GALAYINTLQVTPDASFSLNWTAAAIFIAVLGGIGTLEGPFLGTLLYFFLRESFSAYGAW 242 GA V PD +F + + + + +LGG GT+ GP LG + F+ E ++G W Sbjct: 226 GATYAFLNQYVNPD-TFRFDDSVRFLLMVILGGSGTVIGPALGATILTFIPEYLQSFGEW 284 Query: 243 YFIGLGSLAIFAMLFSPGG 261 G L M P G Sbjct: 285 QRFAYGVLLALVMFGMPMG 303 >gi|134096409|ref|YP_001101484.1| putative high-affinity branched-chain amino acid transport system permease protein braE [Herminiimonas arsenicoxydans] Length = 388 Score = 54.3 bits (129), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 62/267 (23%), Positives = 112/267 (41%), Gaps = 14/267 (5%) Query: 15 GFGGLIAIGQHAFIGVGAYSLFFLSNSLDCAPWLLLPLATLSALAFALLSAWPMFRLSGP 74 GF G+ AF G+G+Y+ L+ + + ++ + A + WP+ R G Sbjct: 115 GFSGVSNFAGAAFFGIGSYTTAVLATHTGLPTLVTIAISGIVAAVIGSILIWPVLRTRGH 174 Query: 75 YFAVGTWVLAEMLRIAAQNTDVLGAGSGLSLDEM--------NNIDRWTRNAGSY----W 122 Y A+ T + + + DVLG GL + M +N + Y Sbjct: 175 YAALVTIAFGILFKTFLEVNDVLGGPQGLQVPGMTLFGHAFNDNFTLFGVEFSFYVNYVL 234 Query: 123 AALALCISAVVVARMTLRGRIGLALMSVRDSEVAATACGVSVRKAKLWIWLIAAAMTGTA 182 +L L + V+ + R +GL++ VR E AA+ G+ + + K+ + + G A Sbjct: 235 LSLLLFVCVFVLVKALERSWVGLSMDVVRTDETAASTFGLHIVRWKVIAFTLGNLFAGVA 294 Query: 183 GALAYINTLQVTPDASFSLNWTAAAIFIAVLGGIGTLEGPFLGTLLYFFLRESFSAYGAW 242 G++ + V P+ +F+ + + I +LGG+G + G ++ L E + Sbjct: 295 GSIYGMMVGFVAPN-NFTFADSLLLLSITILGGLGNVIGLIPAAIIVLMLPEKLQFIQEY 353 Query: 243 YFIGLGSLAIFAMLFSPGGAWAPIKRR 269 + +L I +LF P G P K R Sbjct: 354 RVLLYAALVIAILLFRPDG-LLPRKTR 379 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.326 0.138 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 521,704 Number of extensions: 19604 Number of successful extensions: 91 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 4 Length of query: 308 Length of database: 1,000,347 Length adjustment: 81 Effective length of query: 227 Effective length of database: 733,452 Effective search space: 166493604 Effective search space used: 166493604 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)