BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4192.p (466 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096615|ref|YP_001101690.1| tRNA modification GTPase TrmE [... 738 0.0 gi|134094495|ref|YP_001099570.1| GTP-binding protein EngA [Hermi... 69 7e-13 gi|134094497|ref|YP_001099572.1| GTPase [Herminiimonas arsenicox... 62 4e-11 >gi|134096615|ref|YP_001101690.1| tRNA modification GTPase TrmE [Herminiimonas arsenicoxydans] Length = 466 Score = 738 bits (1905), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/469 (78%), Positives = 406/469 (86%), Gaps = 6/469 (1%) Query: 1 MTLDSSPIAAIATAPGRGGIGVVRISGRNLTPVMRAVCGRE---SLAPRHATYLPFQNED 57 MT DSSPIAAIATAPGRGGIGVVRISG++L+ VMRAVCG + SL RHATYL F N D Sbjct: 1 MTFDSSPIAAIATAPGRGGIGVVRISGKDLSSVMRAVCGADKGSSLQARHATYLDFVNSD 60 Query: 58 GSVIDQGLAIYFPGPHSYTGEDVLELQGHGGPVVMQMLLARCVQAGSEIGLRLAEPGEFT 117 GSVIDQGLAIYF PHSYTGEDVLELQGHGGPVV+QMLL RC++AG++IGLR+AEPGEFT Sbjct: 61 GSVIDQGLAIYFKAPHSYTGEDVLELQGHGGPVVLQMLLTRCLEAGADIGLRMAEPGEFT 120 Query: 118 QRAFLNDKIDLAQAEAVADLIEATTEAAAKSASESLSGAFSKVIHELVDQVTRLRMLVEA 177 RAFLNDK+DLAQAE V DLIEA+TEAAAKSA++SLSGAFSK I ELVD++T LRMLVEA Sbjct: 121 HRAFLNDKLDLAQAEGVIDLIEASTEAAAKSATQSLSGAFSKTIQELVDKITNLRMLVEA 180 Query: 178 TLDFPEEEIDFLQQSDARGQLATIRSTLEDVFKHAAQGALLRDGLNAVLAGKPNVGKSSL 237 TLDFPEEEIDFL++SDARGQL IR L+ VF AAQGALLRDGLN VLAG+PNVGKSSL Sbjct: 181 TLDFPEEEIDFLEKSDARGQLTNIREALQRVFSQAAQGALLRDGLNIVLAGQPNVGKSSL 240 Query: 238 LNVLAGSDVAIVTPIAGTTRDKVTQTIQIEGMPLNIIDTAGIREAGEDGSGPDEVERIGI 297 LN LAGSDVAIVTPIAGTTRDKV +TIQIEG+P+N+IDTAGIR+A + G DEVERIGI Sbjct: 241 LNALAGSDVAIVTPIAGTTRDKVIETIQIEGIPVNVIDTAGIRDAADAG---DEVERIGI 297 Query: 298 ERTWAEVAKADVILHMLDADRGPTLEDEKITARFPDGVPIIRIWNKIDRSGHKPAVDKMS 357 ERTWA V ADVI+HMLDA RGPT DE+IT RFP VP++R+WNKID SGH+PA+D+M Sbjct: 298 ERTWAAVQTADVIVHMLDASRGPTRADEQITERFPANVPVMRVWNKIDLSGHRPAIDRMP 357 Query: 358 DATHVYLSAAEGQGVDLLRAELLRIAGWQQTVESRYLARERHLLALKAADEHLAHAAAHA 417 DATH+YLSA G+DLLR ELL + GWQQT ES YLARERHL+ALK+A HL AA HA Sbjct: 358 DATHIYLSATGLLGMDLLRTELLHLVGWQQTGESLYLARERHLVALKSAHSHLEMAAQHA 417 Query: 418 ALDNEAGDHALDLFAEELRLAQERLSSITGKFTSDDLLGVIFSRFCIGK 466 A DNEA D ALDLFAEELRLAQERLSSITG+FTSDDLLGVIFSRFCIGK Sbjct: 418 AHDNEATDPALDLFAEELRLAQERLSSITGEFTSDDLLGVIFSRFCIGK 466 >gi|134094495|ref|YP_001099570.1| GTP-binding protein EngA [Herminiimonas arsenicoxydans] Length = 447 Score = 68.6 bits (166), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 37/102 (36%), Positives = 61/102 (59%), Gaps = 3/102 (2%) Query: 226 LAGKPNVGKSSLLNVLAGSDVAIVTPIAGTTRDKVTQTIQIEGMPLNIIDTAGIREAGED 285 + G+PNVGKS+L+N L G + I + GTTRD + + EG +IDTAGIR G+ Sbjct: 185 IVGRPNVGKSTLVNTLLGEERVIAFDLPGTTRDSIEIPFEREGKLYTLIDTAGIRRRGKV 244 Query: 286 GSGPDEVERIGIERTWAEVAKADVILHMLDADRGPTLEDEKI 327 + +E+ + +T +++A+V+L +LDA + + +D I Sbjct: 245 F---EAIEKFSVVKTLQSISEANVVLLLLDAQQDISEQDAHI 283 Score = 59.7 bits (143), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 52/169 (30%), Positives = 80/169 (47%), Gaps = 9/169 (5%) Query: 226 LAGKPNVGKSSLLNVLAGSDVAIVTPIAGTTRDKVTQTIQIEGMPLNIIDTAGIREAGED 285 L G+PNVGKS+L N L S A+V + G TRD+ ++ P +IDT G ++ Sbjct: 7 LVGRPNVGKSTLFNRLTRSRDALVADLPGLTRDRHYGEGRMGERPFLVIDTGGFEPVAKE 66 Query: 286 GSGPDEVERIGIERTWAEVAKADVILHMLDADRGPTLEDEKITARF-PDGVPIIRIWNKI 344 G + ++T VA+ADV++ ++D +G T D+ IT G ++ + NK Sbjct: 67 G-----IMHEMAKQTKQAVAEADVVIFIVDGRQGLTPHDKTITDFLRKSGRSVMLVVNKA 121 Query: 345 DRSGHKPAVDKMSD---ATHVYLSAAEGQGVDLLRAELLRIAGWQQTVE 390 + + + +SAA G GV L E L IA Q+ E Sbjct: 122 EGMKYTMVTADFYELGLGDPYVISAAHGDGVSDLVEESLDIAFAQRPPE 170 >gi|134094497|ref|YP_001099572.1| GTPase [Herminiimonas arsenicoxydans] Length = 338 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 60/192 (31%), Positives = 91/192 (47%), Gaps = 20/192 (10%) Query: 197 QLATIRSTLEDVFKHAAQGALLRDGLNAV---LAGKPNVGKSSLLNVLAGSDVAIVTPIA 253 ++ +R+ LE + K R NA L G N GKS+L N LA + + + Sbjct: 128 RVKALRAKLEKLHKQHITQRRARGRNNAFSVSLVGYTNAGKSTLFNALAKTKMLAADQLF 187 Query: 254 GTTRDKVTQTIQI-EGMPLNIIDTAG-IREAGEDGSGPDEVERIGIERTWAEVAKADVIL 311 T D ++ I + E + I DT G IRE P ++ T E AD++L Sbjct: 188 ATL-DTTSRRIYLGEAGNVVISDTVGFIREL------PHQLV-AAFRATLEETIHADLLL 239 Query: 312 HMLDADRGPTLED-EKITARF----PDGVPIIRIWNKIDRSGHKPAV--DKMSDATHVYL 364 H++D +E E++ A D +P I +WNKID +G +PA+ D+ V++ Sbjct: 240 HVVDGASPARMEQIEEVNAVLREIGADQIPQILVWNKIDAAGLEPALERDEYDKIRRVFI 299 Query: 365 SAAEGQGVDLLR 376 SA G G+DLLR Sbjct: 300 SAQTGSGLDLLR 311 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.317 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 801,101 Number of extensions: 32779 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 4 Length of query: 466 Length of database: 1,000,347 Length adjustment: 85 Effective length of query: 381 Effective length of database: 720,272 Effective search space: 274423632 Effective search space used: 274423632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)