BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4168.p (328 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134096605|ref|YP_001101680.1| glyoxylate reductase [Herminiim... 471 e-134 gi|134094049|ref|YP_001099124.1| putative glyoxylate reductase (... 139 3e-34 gi|134096490|ref|YP_001101565.1| putative phosphoglycerate dehyd... 105 3e-24 gi|134096368|ref|YP_001101443.1| phosphonate dehydrogenase [Herm... 66 3e-12 gi|134096589|ref|YP_001101664.1| formate dehydrogenase [Herminii... 62 6e-11 >gi|134096605|ref|YP_001101680.1| glyoxylate reductase [Herminiimonas arsenicoxydans] Length = 327 Score = 471 bits (1211), Expect = e-134, Method: Compositional matrix adjust. Identities = 235/325 (72%), Positives = 273/325 (84%) Query: 1 MKIAILDDYQDAVRHLDCFSLLAGHDVKVFNNTVRGIGQLTARLSPFDAVVLIRERTALT 60 MKIAILDDYQD VR LDCFSLL GH+VKVF N+ RG+GQL RL+ FDA+VLIRERTAL+ Sbjct: 1 MKIAILDDYQDCVRQLDCFSLLDGHEVKVFTNSARGVGQLAIRLADFDALVLIRERTALS 60 Query: 61 RPLLEKLPLLKLISQTGKVSGHVDVAAATERGIAIVEGVGDPTAPAELTWALIMAAMRRV 120 RPLLEKLP LKLISQTGKVSGH+D++AA R I IVEGVGDPTAPAELTWALIMAAMR++ Sbjct: 61 RPLLEKLPKLKLISQTGKVSGHIDLSAAAARNITIVEGVGDPTAPAELTWALIMAAMRKL 120 Query: 121 PQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGKLVAGYGRAFGMSVLIWG 180 PQY L+ G WQ S + HN +G LKGRTLGIWGYG+IG++VAGYG+AFGM VL+WG Sbjct: 121 PQYVGNLKDGLWQIASLSSAHNTLGAVLKGRTLGIWGYGRIGRMVAGYGQAFGMKVLVWG 180 Query: 181 REASREQAVKDGYQAAASKEEFFAQADVLSLHLRLNDATRAIVKAADLARMKPTACLVNT 240 R+ASR AV+DGYQAA+S+EEFF+ ADVLSLHLRLNDATR IV A+DLA MK +A VNT Sbjct: 181 RDASRAAAVQDGYQAASSREEFFSTADVLSLHLRLNDATRGIVTASDLAHMKSSALFVNT 240 Query: 241 SRAELVEPEALEAALQAGRPGLAALDVYEREPLPVESPLLQMDNVVAAPHLGYVEKDGYE 300 SRAELV +AL+ AL+ GRPG AALDV+E EPL S LL+M+NV+A+PHLGYVEKD YE Sbjct: 241 SRAELVASDALQTALEQGRPGYAALDVFESEPLAPTSSLLRMENVLASPHLGYVEKDSYE 300 Query: 301 LYFRAAFQNIVDYAAGRPANVLNPE 325 LYFR+AF+NIV +A G P NVL P+ Sbjct: 301 LYFRSAFENIVAFANGAPKNVLLPK 325 >gi|134094049|ref|YP_001099124.1| putative glyoxylate reductase (glycolate reductase) [Herminiimonas arsenicoxydans] Length = 327 Score = 139 bits (349), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 103/280 (36%), Positives = 139/280 (49%), Gaps = 9/280 (3%) Query: 51 VLIRERTALTRPLLEKLPLLKLISQTGKVSGHVDVAAATERGIAIVE--GVGDPTAPAEL 108 V+I L+ ++ P LK I ++DVAAAT+ GI + V + T A+ Sbjct: 49 VIITSSEKLSGEVITANPQLKAICNVAVGYNNIDVAAATKAGIMVTNTPDVLNETT-ADY 107 Query: 109 TWALIMAAMRRVPQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGKLVAGY 168 W L+MA RR + LRAG W+ +G + G TLGI G G+IG+ +A Sbjct: 108 AWTLLMANARRTSESEHYLRAGKWKNWR---FDQFLGADVHGATLGILGMGRIGQAIARR 164 Query: 169 GRAFGMSVLIWGREA-SREQAVKDGYQAAASKEEFFAQADVLSLHLRLNDATRAIVKAAD 227 F M V+ R + +Q SKEE AD L L L ++ T I+ AA+ Sbjct: 165 SMGFDMQVIYHNRSRLTPDQEAHANNARYVSKEELLRTADHLILVLPYSEQTHHIIGAAE 224 Query: 228 LARMKPTACLVNTSRAELVEPEALEAALQAGRPGLAALDVYEREPLPVESPLLQMDNVVA 287 LA MKPTA LVN +R +V+ AL AAL+ R A LDVYE EP + L + NVV Sbjct: 225 LALMKPTATLVNIARGGIVDDVALIAALREHRIASAGLDVYENEP-ALHPDFLTLSNVVL 283 Query: 288 APHLGYVEKDGYELYFRAAFQNIVDYAAG-RPANVLNPEV 326 PH+G + A N+V +G RP N+LNPEV Sbjct: 284 TPHIGSASEKTRRAMSDCASLNMVAALSGQRPPNLLNPEV 323 >gi|134096490|ref|YP_001101565.1| putative phosphoglycerate dehydrogenase(partial) [Herminiimonas arsenicoxydans] Length = 317 Score = 105 bits (262), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 87/260 (33%), Positives = 134/260 (51%), Gaps = 8/260 (3%) Query: 40 LTARLSPFDAVVLIRERTALTRPLLEKLPLLKLISQTGKVSGHVDVAAATERGIAIVEGV 99 L A L DA++ +R RT + LL+ P L+++ + G ++DV A RGI + Sbjct: 44 LLAMLGNVDALI-VRNRTKVDIALLDAAPKLRVVGRLGVGLDNIDVDACKARGIDVFPAT 102 Query: 100 G-DPTAPAELTWALIMAAMRRVPQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGY 158 G + A AE MA +R + AG W + + + G + G+TLG+ G+ Sbjct: 103 GANAGAVAEYVIGTAMALLREAYTRSAETAAGTWPRTALSN-----GREIAGKTLGLIGF 157 Query: 159 GKIGKLVAGYGRAFGMSVLIWGREASREQAV-KDGYQAAASKEEFFAQADVLSLHLRLND 217 G IG+L A +A G++V+ + + A+ + A E QAD++SLH+ L Sbjct: 158 GGIGQLTARLAQALGVTVIAHDPLLAPDAAIWQQAGVACKQLHEVLEQADIVSLHIPLTT 217 Query: 218 ATRAIVKAADLARMKPTACLVNTSRAELVEPEALEAALQAGRPGLAALDVYEREPLPVES 277 TR ++ A LARMKP A L+NTSR E+V+ AL AL + G AA+DV+ +EPL + Sbjct: 218 NTRNLIGADALARMKPDAVLINTSRGEIVDEAALAQALHSANLGGAAIDVFAQEPLAPGN 277 Query: 278 PLLQMDNVVAAPHLGYVEKD 297 L V+ PH+ V + Sbjct: 278 ALANAPRVILTPHIAGVTTE 297 >gi|134096368|ref|YP_001101443.1| phosphonate dehydrogenase [Herminiimonas arsenicoxydans] Length = 336 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 76/274 (27%), Positives = 121/274 (44%), Gaps = 20/274 (7%) Query: 63 LLEKLPLLKLISQTGKVSGHVDVAAATERGI---AIVEGVGDPTAPAELTWALIMAAMRR 119 L P LK++ K + DV A T+RG+ + + + PTA ELT L++ R Sbjct: 61 FLAACPKLKIVFAALKGYDNFDVDACTKRGVWFGIVPDLLTVPTA--ELTVGLLLGLTRH 118 Query: 120 VPQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGKLVAGYGRAFGMSVLIW 179 V +R+G + P + G L G T+GI G G++GK +A F M+ ++ Sbjct: 119 VMAGDDHVRSGTFHGWRP----KLYGAGLAGSTIGIIGMGRVGKAIAKRLSGFEMNA-VY 173 Query: 180 GREASREQAVKDGYQA-AASKEEFFAQADVLSLHLRLNDATRAIVKAADLARMKPTACLV 238 + + A S +E +D + L + T ++ A +++M+ + L+ Sbjct: 174 CDSVPLNPVDEQAWNARQVSFDELLTCSDFVVPMLPMTSDTFHLIDAHAISKMRRGSYLL 233 Query: 239 NTSRAELVEPEALEAALQAGRPGLAALDVYERE-------PLPVESPLL-QMDNVVAAPH 290 NTSR +V+ A+ AL G A DV+E E PL V LL + PH Sbjct: 234 NTSRGSVVDENAVVEALNQGHLAGYAADVFEMEEWARPDRPLTVPQALLNNRTQTLFTPH 293 Query: 291 LGY-VEKDGYELYFRAAFQNIVDYAAGRPANVLN 323 +G V+K E+ +A + A RP LN Sbjct: 294 VGSGVKKVRLEIERYSAHSILQALAGQRPDGALN 327 >gi|134096589|ref|YP_001101664.1| formate dehydrogenase [Herminiimonas arsenicoxydans] Length = 400 Score = 61.6 bits (148), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 67/278 (24%), Positives = 113/278 (40%), Gaps = 19/278 (6%) Query: 46 PFDAVVLIRERTALTRPLLEKLPLLKLISQTGKVSGHVDVAAATERGIAIVE-GVGDPTA 104 PF + ER A K LK+I G S H D+ AA + I + E + + Sbjct: 98 PFWPAYMTAERIA-------KAKKLKMIVTAGIGSDHTDLEAANKHNITVAEVTYCNSHS 150 Query: 105 PAELTWALIMAAMRR-VPQYCSALRAGAWQQVSPTPHHNMIGTALKGRTLGIWGYGKIGK 163 AE +I++ +R +P Y + G W L+ ++G G+IG Sbjct: 151 VAEHVLMMILSQVRNYIPSYKQVIDGG-WNIADCVSR----SYDLEAMSVGTVAAGRIGL 205 Query: 164 LVAGYGRAFGMSVLIWGRE---ASREQAVKDGYQAAASKEEFFAQADVLSLHLRLNDATR 220 V F + + R + E+ + Y + + E DV++L+ L+ T Sbjct: 206 RVLRLLHPFDVKLHYMDRHRLPTAVEKELNLTYHS--TLESLTKVCDVVTLNCPLHPETE 263 Query: 221 AIVKAADLARMKPTACLVNTSRAELVEPEALEAALQAGRPGLAALDVYEREPLPVESPLL 280 ++ L K A ++NT+R +L + +A+ AAL++G+ A DV+ +P P P Sbjct: 264 HMINEKTLKNFKRGAYIINTARGKLCDRDAIVAALKSGQLAGYAGDVWFPQPAPKNHPWR 323 Query: 281 QMDNVVAAPHLGYVEKDGYELYFRAAFQNIVDYAAGRP 318 M + PH+ Y + + Y GRP Sbjct: 324 TMPHHGMTPHISGTSLTAQTRYAAGTREILECYFEGRP 361 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 586,522 Number of extensions: 22458 Number of successful extensions: 84 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 76 Number of HSP's successfully gapped: 5 Length of query: 328 Length of database: 1,000,347 Length adjustment: 82 Effective length of query: 246 Effective length of database: 730,157 Effective search space: 179618622 Effective search space used: 179618622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)