BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.4002.p (262 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimo... 189 1e-49 gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/is... 112 2e-26 gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimo... 91 6e-20 >gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 258 Score = 189 bits (481), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 110/260 (42%), Positives = 162/260 (62%), Gaps = 10/260 (3%) Query: 5 LPHDDILVARHGEQVLHLTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISGNDQ 64 + +++IL+ HG +V +TLNRPA NAL ++ + E+ + L + ++ + ++I+G+++ Sbjct: 1 MQYENILIEIHG-KVGLITLNRPAALNALNDKLMDELGKALLSFDANEDIGCMIITGSEK 59 Query: 65 AFAAGADLNEMIHKDAIATQLDV-RAQY----WRAIARFPKPLLAAVNGYALGAGCELLM 119 AFAAGAD+ M A + +DV + Y W I KP++AAV GYALG GCEL M Sbjct: 60 AFAAGADIGAM----ATYSYMDVYKGDYITRNWEQIRSVRKPVIAAVAGYALGGGCELAM 115 Query: 120 HADIAIAGRSARIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAG 179 D IA +A+ GQPEI +G +PGAGGTQRL R+V K AM M L+ + A +A +AG Sbjct: 116 MCDFIIAADTAKFGQPEIKLGIIPGAGGTQRLPRSVSKSKAMDMCLTARMMDAAEAERAG 175 Query: 180 LVAEVVEDEATLERSLALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMA 239 LV+ VV + E +LA AT IA S V + KE++ +++E L G+ ER+ F Sbjct: 176 LVSRVVAADKLQEEALAAATVIASMSLPMVMMIKESINRAYETTLAEGVQFERRLFHSTF 235 Query: 240 ASADRQEGIAAFQEKRAAVF 259 A+ D++EG+ AF EKRAA F Sbjct: 236 ATEDQKEGMRAFVEKRAAKF 255 >gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/isomerase family protein [Herminiimonas arsenicoxydans] Length = 301 Score = 112 bits (280), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 83/256 (32%), Positives = 131/256 (51%), Gaps = 6/256 (2%) Query: 9 DILVARHGEQVLHLTLNRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISGNDQAFAA 68 +I +RH E L LTL+ P +NAL ++ + A +DDS+R V+++G D F A Sbjct: 46 EIRASRH-EATLILTLSNPGAKNALHPDMFAAAIETFSTAARDDSIRAVILTGADNFFCA 104 Query: 69 GADLNEMIHKDAI-----ATQLDVRAQYWRAIARFPKPLLAAVNGYALGAGCELLMHADI 123 G +LN ++ A A +D + AI PKP++AAV A GAG L + D+ Sbjct: 105 GGNLNTLLELRAQDRADQADAVDSLRGWIEAIRDCPKPVIAAVESAAAGAGFSLALACDL 164 Query: 124 AIAGRSARIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQAGLVAE 183 +AG +A+ +N+G P G + L + + + LA +++L G+ + A++ G++ Sbjct: 165 IVAGANAKFAMSYVNIGLTPDGGASWFLSQALPRQLATEIMLEGKPVPAQRLHDLGVINR 224 Query: 184 VVEDEATLERSLALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLMAASAD 243 +V D ATL+ SL A +A S AV K ++ + L +E+ F D Sbjct: 225 IVADGATLQTSLDWADELAALSGHAVERIKSLTREARDHSLPQHFEIEKNHFVDSLNHRD 284 Query: 244 RQEGIAAFQEKRAAVF 259 QEGI AF EKRA + Sbjct: 285 AQEGIHAFLEKRAPHY 300 >gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 264 Score = 90.9 bits (224), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 77/263 (29%), Positives = 125/263 (47%), Gaps = 20/263 (7%) Query: 14 RHGEQVLHLTL---------NRPAVRNALRNQSLQEIVQELARAEQDDSVRVVVISG-ND 63 + GE +HL + +RP RNA+ E+ R D S+RV G Sbjct: 6 KTGEGRVHLAIQDGIAAILFDRPQARNAMTWAMYTELGAICERIRADQSIRVTTFRGAGG 65 Query: 64 QAFAAGADLNEMIH----KDAIATQLDVRAQYWRAIARFPKPLLAAVNGYALGAGCELLM 119 +AF AG D+ + + +D IA + V + + I P P LA V+G+A+G G + Sbjct: 66 EAFVAGTDIEQFRNFSSGEDGIAYERIVDERIGQ-IEALPMPTLAIVDGWAIGGGLAIAA 124 Query: 120 HADIAIAGRSARIGQP-EINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFITAEQALQA 178 D IA ++R G P +G R++ G A +++L E + A++ L A Sbjct: 125 ACDFRIATPASRFGIPIARTLGNCLSMANIARVVAAFGVGRAKRILLLAEVLAADELLAA 184 Query: 179 GLVAEVVEDEATLERSLALATRIAQKSPLAVRLAKEAMLQSFELGLEAGLLLERKSFSLM 238 G V EVVE + AL ++A +P+ + ++KEA+ + + GL G L R + Sbjct: 185 GFVTEVVEAAVLDATADALCKKLASNAPITMGVSKEAIRRLTQAGLPDGEDLIRACYG-- 242 Query: 239 AASADRQEGIAAFQEKRAAVFSG 261 S+D + G+ AF EK+ ++SG Sbjct: 243 --SSDFKIGVHAFLEKQRPLWSG 263 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.133 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 437,302 Number of extensions: 16670 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 5 Length of query: 262 Length of database: 1,000,347 Length adjustment: 80 Effective length of query: 182 Effective length of database: 736,747 Effective search space: 134087954 Effective search space used: 134087954 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)