BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3895.p (259 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimo... 101 5e-23 gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimo... 82 2e-17 gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/is... 59 3e-10 >gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 258 Score = 101 bits (251), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 77/261 (29%), Positives = 127/261 (48%), Gaps = 12/261 (4%) Query: 3 QHSDLLDIQVHGAIAEIALNRSAKRNALNDPMVRAIRDCF--EDLPPEVKVAIIHGRGEH 60 Q+ ++L I++HG + I LNR A NALND ++ + D ++ II G + Sbjct: 2 QYENIL-IEIHGKVGLITLNRPAALNALNDKLMDELGKALLSFDANEDIGCMIITGSEKA 60 Query: 61 FCAGLDLSELRER---DTTESVHHSRMWHAAFEQISFGRVPVIAVLHGAVIGGGLELASA 117 F AG D+ + D + + +R W EQI R PVIA + G +GGG ELA Sbjct: 61 FAAGADIGAMATYSYMDVYKGDYITRNW----EQIRSVRKPVIAAVAGYALGGGCELAMM 116 Query: 118 AHIRVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVGI 177 +A +A +G PE + G+ G GG+ R+ R + ++ DM LT R++ A E + G+ Sbjct: 117 CDFIIAADTAKFGQPEIKLGIIPGAGGTQRLPRSVSKSKAMDMCLTARMMDAAEAERAGL 176 Query: 178 SQYSVAEGAGLDKARELAERIAR-NAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAG 236 VA ++A A IA + PM + + R ++ ++ +G+ E + Sbjct: 177 VSRVVAADKLQEEALAAATVIASMSLPMVMM-IKESINRAYETTLAEGVQFERRLFHSTF 235 Query: 237 SDPDTKGRLAAFLDHKQNKVK 257 + D K + AF++ + K K Sbjct: 236 ATEDQKEGMRAFVEKRAAKFK 256 >gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 264 Score = 82.4 bits (202), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 72/250 (28%), Positives = 117/250 (46%), Gaps = 11/250 (4%) Query: 10 IQVHGAIAEIALNRSAKRNALNDPMVRAIRDCFEDLPPEVKVAIIHGRG---EHFCAGLD 66 + + IA I +R RNA+ M + E + + + + RG E F AG D Sbjct: 14 LAIQDGIAAILFDRPQARNAMTWAMYTELGAICERIRADQSIRVTTFRGAGGEAFVAGTD 73 Query: 67 LSELRERDTTES-VHHSRMWHAAFEQISFGRVPVIAVLHGAVIGGGLELASAAHIRVAEP 125 + + R + E + + R+ QI +P +A++ G IGGGL +A+A R+A P Sbjct: 74 IEQFRNFSSGEDGIAYERIVDERIGQIEALPMPTLAIVDGWAIGGGLAIAAACDFRIATP 133 Query: 126 SAFYGLPEGQR-GLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVGISQYSVAE 184 ++ +G+P + G + R+ GV R ++L VL+A+E G V E Sbjct: 134 ASRFGIPIARTLGNCLSMANIARVVAAFGVGRAKRILLLAEVLAADELLAAGFVT-EVVE 192 Query: 185 GAGLD-KARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKG 243 A LD A L +++A NAP+T + R+ + DG E L+ A GS D K Sbjct: 193 AAVLDATADALCKKLASNAPITMGVSKEAIRRLTQAGLPDG---EDLIRACYGSS-DFKI 248 Query: 244 RLAAFLDHKQ 253 + AFL+ ++ Sbjct: 249 GVHAFLEKQR 258 >gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/isomerase family protein [Herminiimonas arsenicoxydans] Length = 301 Score = 58.9 bits (141), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 56/195 (28%), Positives = 89/195 (45%), Gaps = 11/195 (5%) Query: 13 HGAIAEIALNRSAKRNALNDPMVRAIRDCFEDLPPE--VKVAIIHGRGEHFCAGLDLSEL 70 H A + L+ +NAL+ M A + F + ++ I+ G FCAG +L+ L Sbjct: 52 HEATLILTLSNPGAKNALHPDMFAAAIETFSTAARDDSIRAVILTGADNFFCAGGNLNTL 111 Query: 71 RE------RDTTESVHHSRMWHAAFEQISFGRVPVIAVLHGAVIGGGLELASAAHIRVAE 124 E D ++V R W E I PVIA + A G G LA A + VA Sbjct: 112 LELRAQDRADQADAVDSLRGW---IEAIRDCPKPVIAAVESAAAGAGFSLALACDLIVAG 168 Query: 125 PSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLSAEEGHQVGISQYSVAE 184 +A + + GL GG S +S+ + ++ML G+ + A+ H +G+ VA+ Sbjct: 169 ANAKFAMSYVNIGLTPDGGASWFLSQALPRQLATEIMLEGKPVPAQRLHDLGVINRIVAD 228 Query: 185 GAGLDKARELAERIA 199 GA L + + A+ +A Sbjct: 229 GATLQTSLDWADELA 243 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 504,435 Number of extensions: 21470 Number of successful extensions: 79 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 79 Number of HSP's successfully gapped: 3 Length of query: 259 Length of database: 1,000,347 Length adjustment: 80 Effective length of query: 179 Effective length of database: 736,747 Effective search space: 131877713 Effective search space used: 131877713 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)