BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3767.p (566 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 827 0.0 gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 130 2e-31 gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Hermi... 101 1e-22 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 827 bits (2137), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/556 (71%), Positives = 465/556 (83%) Query: 1 MDRFWLASYPAGVPADIDPSPYGSLVQMIDEAFVKHADRNAYVCMGKYLRYRELDAMSRA 60 MD+FWL SYP GVPA+ID + Y SLV++++E+F K+ADR+AYVCM K+L Y E+D +S+ Sbjct: 1 MDKFWLQSYPKGVPAEIDFTQYDSLVELLEESFRKYADRHAYVCMDKFLTYAEVDTLSQK 60 Query: 61 LGAWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSG 120 LGAWLQG GLQKGARVA+MMPNVLQYP+A+AA+LRAGY VVNVNPLY REL+HQL DSG Sbjct: 61 LGAWLQGTGLQKGARVALMMPNVLQYPVALAAVLRAGYVVVNVNPLYKPRELQHQLVDSG 120 Query: 121 AEAIFVLENFACTLQKVVAHTQIKHIVVASMGELLGTLKGAIVNLVVRHVKKLVPAWSLP 180 AEAI +LENFA TL+K++ TQIKH+VVA+MG++LG LKG +VN VVRHVKK+VPA+SLP Sbjct: 121 AEAIIILENFATTLEKIIVSTQIKHVVVATMGDMLGGLKGLLVNFVVRHVKKMVPAFSLP 180 Query: 181 NAISFSRALSQAQGQSLQPVLLTPDDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEQW 240 A+SF L+QA LQ LL P DIAFLQYTGGTTG AKGA+LTHRN+VAN+LQ E W Sbjct: 181 QAVSFKDVLAQAAHMHLQRPLLGPQDIAFLQYTGGTTGTAKGAMLTHRNIVANILQNEAW 240 Query: 241 LTPALKSGQPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLIPNPRDIAGFVKELAK 300 + P L+ P L FVCALPLYHIFALT C MLGTR G +N+LIPNPRDI VKEL K Sbjct: 241 IWPVLRKEPPDKTLNFVCALPLYHIFALTACCMLGTRLGVMNILIPNPRDIPSLVKELMK 300 Query: 301 YKVNTFPAVNTLYNALLNNADFAKLDFSGYRCCVGGGMAVQKGVADKWKALTGCPIIEGY 360 YK+N PAVNTLYN LLNN DFAK+DFS + C GGGMAVQ+ V D+W+ +TGC IIEGY Sbjct: 301 YKINLLPAVNTLYNGLLNNPDFAKVDFSELKLCNGGGMAVQQVVNDRWRVVTGCSIIEGY 360 Query: 361 GLSETSPIASANPCTIDDYTGTIGLPLPSTEIAILDDDGQPLPLGQPGEIAIRGPQVMPG 420 GLSETSP+A++NP +++GTIGLP+PSTE+ ILD + + LG GEIAIRGPQVM G Sbjct: 361 GLSETSPVATSNPVETTEFSGTIGLPVPSTEVMILDSNDNEVALGLSGEIAIRGPQVMAG 420 Query: 421 YWQRPDETAKVMTPDGFFKTGDIGIMDARGYTRIVDRKKDMVLVSGFNVYPNEIESVVAL 480 YW P ETA+ MT DGFFKTGDIG+MDARGYTRIVDRKKDM+LVSGFNVYP+EIE+VVA Sbjct: 421 YWNNPAETAQAMTADGFFKTGDIGVMDARGYTRIVDRKKDMILVSGFNVYPSEIENVVAG 480 Query: 481 HPGVLECACVGVPDEHTGEAVKLFVVRKDKSLTTEQLMAYCKEQFTGYKKPKYIEFRDEL 540 HPGVLECAC+GVPDEHTGEAVKLFVVR+D +LT E LMA+CKEQFTGYKKPK IEFR EL Sbjct: 481 HPGVLECACIGVPDEHTGEAVKLFVVRRDPALTAEALMAFCKEQFTGYKKPKMIEFRTEL 540 Query: 541 PKTNVGKILRRELRDE 556 PK+NVGKILRRELRDE Sbjct: 541 PKSNVGKILRRELRDE 556 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 144/549 (26%), Positives = 226/549 (41%), Gaps = 73/549 (13%) Query: 49 LRYRELDAMSRALGAWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYT 108 + Y+EL L+ L + KG RV I MP + A+ A R G T V ++ Sbjct: 114 ITYKELHERVCKFANGLKSLSINKGDRVIIYMPMSIDGIAAMQACARIGATHSVVFGGFS 173 Query: 109 ARELEHQLKDSGAEAIFVLE-----NFACTLQKVVAHT-------QIKHIVVASMGELLG 156 A+ L ++ D+GA A+ + L+ +V I+++VV Sbjct: 174 AKSLHERIIDAGAVAVLTADYQVRGGKNLPLKAIVDEALAMGGCDTIRNVVVYKR----- 228 Query: 157 TLKGAIVNLVVRHVKKLVPAWSLPNAISFSRALSQAQGQSLQPVLLTPDDIAFLQYTGGT 216 G V+ V R L L + + P + + F+ YT G+ Sbjct: 229 --TGGAVDFVERRDIWL-------------HELVEQRTSECAPEPVGAEHPLFILYTSGS 273 Query: 217 TGVAKGAVLTHRNLVANVLQAEQWLTPALKSGQPIDQLVFVCALPLYHIFALTVCNMLGT 276 TG KG + + + +W+ +P D V+ C + + T Sbjct: 274 TGKPKGVQHSSAGYLLWAMLTMKWVFDI----KPND--VYWCTADIGWVTGHTYVTYGPL 327 Query: 277 REGALNLL---IPNPRDIAGFVKELAKYKVNTFPAVNTLYNALLNNADF------AKLDF 327 GA ++ +P + F + + K+KV+ F T +L+ A+ +K D Sbjct: 328 AVGATQIVFEGVPMYPNAGRFWEMVQKHKVSIFYTAPTAIRSLIKAAEADPSTHPSKYDL 387 Query: 328 SGYRCCVGGGMAVQ-KGVADKWKALTG--CPIIEGYGLSET-----SPIASANPCTIDDY 379 S R G + + +K + G CPI++ + +ET SP+ A P Sbjct: 388 SSLRLLGSVGEPINPEAWMWYYKNIGGERCPIVDTFWQTETGGHVISPLPGATPMV---- 443 Query: 380 TGTIGLPLPSTEIAILDDDGQPLPLGQPGEIAIR--GPQVMPGYWQRPDETAKVMTPDGF 437 G+ LPLP A++DD G LP G+ G + I+ P ++ W P+ K P Sbjct: 444 PGSCTLPLPGIIAAVVDDGGHDLPNGKGGLLVIKRPWPAMIRNIWNDPERFEKSYFPHEL 503 Query: 438 ----FKTGDIGIMDAR-GYTRIVDRKKDMVLVSGFNVYPNEIESVVALHPGVLECACVGV 492 + GD I + GY I R D++ +SG + EIES + +P V E A VG Sbjct: 504 GGRTYLAGDGAIRNEETGYFTITGRIDDVLNISGHRMGTAEIESALVANPMVAEAAVVGK 563 Query: 493 PDEHTGEAVKLFVVRK-------DKSLTTEQLMAYCKEQFTGYKKPKYIEFRDELPKTNV 545 PDE TGE++ FVV K + ++L + ++ KPK I F D LPKT Sbjct: 564 PDETTGESICAFVVLKRVRPEGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRS 623 Query: 546 GKILRRELR 554 GKI+RR LR Sbjct: 624 GKIMRRLLR 632 >gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 634 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 136/573 (23%), Positives = 226/573 (39%), Gaps = 78/573 (13%) Query: 29 IDEAFVKHADRNAYVCMG------KYLRYRELDAMSRALGAWLQGLGLQKGARVAIMMPN 82 +D AD+ A + + K + +L A A +Q LG+ +G RV I MP Sbjct: 63 VDRHLATRADQPALIAISTETDSEKVYTFAQLHAEVNRCAAMMQSLGVSQGDRVLIYMPM 122 Query: 83 VLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDS----------GAEAIFVLENFAC 132 V + A+ A R G V + + L ++ D+ G+ V+ + Sbjct: 123 VAEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPVLILSADAGSRGCHVMPYKSL 182 Query: 133 TLQKVV-AHTQIKHIVVASMG-ELLGTLKGAIVN---LVVRHVKKLVPAWSLPNAISFSR 187 + +V A + H+++ + G + T+ G ++ L +H+ VP Sbjct: 183 LDEAIVLAEHKPTHVLMVNRGLAPMQTVAGRDIDYATLREKHMDAAVPV----------- 231 Query: 188 ALSQAQGQSLQPVLLTPDDIAFLQYTGGTTGVAKGAVLTHRNL--VANVLQAEQWLTPAL 245 V L + +++ YT GTTG KG V R++ A L L Sbjct: 232 ------------VWLESNAPSYILYTSGTTGKPKGVV---RDVGGYAVALTTSMDLIFCG 276 Query: 246 KSGQPIDQLVFVCALPLYHIFA---LTVCNMLGTREGALNLLIPNPRDIAGFVKELAKYK 302 K+G+ F C + + + +L L +P D + + + KYK Sbjct: 277 KAGE-----TFFCTSDIGWVVGHSYIIYGPLLAGMATVLYEGLPIRPDASIWWSIVEKYK 331 Query: 303 VNTFPAVNTLYNALLNN--ADFAKLDFSGYRCCVGGGMAVQKGVADKWKALTGCPIIEGY 360 V + T L K D S + G + + ++ + P+I+ Y Sbjct: 332 VTRMSSAPTAIRVLKKQPLELLKKHDLSSLKALYLAGEPLDQTTSEWISGVLNVPVIDNY 391 Query: 361 GLSETS-PIASANPCTIDDYT--GTIGLPLPSTEIAILDD-DGQPLPLGQPGEIAIRGP- 415 +ET PI S D T G+ GLPL + I+++ G+ + G + I GP Sbjct: 392 WQTETGWPIISIAKGVGDKPTRSGSPGLPLYGYKTKIINEISGEECAANEKGVVIIEGPL 451 Query: 416 -----QVMPGYWQRPDETAKVMTPDGFFKTGDIGIMDARGYTRIVDRKKDMVLVSGFNVY 470 Q + G QR T + T D GI DA GY I+ R D++ V+G + Sbjct: 452 PPGCLQTLYGDDQRFVSTYWRNFKREAYSTFDWGIRDADGYYFILGRTDDVINVAGHRLG 511 Query: 471 PNEIESVVALHPGVLECACVGVPDEHTGEAVKLFVVRKDKSLTTE---------QLMAYC 521 EIE ++ H V E A VGV + G+ FV+ K+ +L ++M Sbjct: 512 TREIEESISSHGNVSEVAVVGVENPIKGQCAVAFVILKNAALAASPEEAKKLEAEIMKVV 571 Query: 522 KEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 ++ +P ++ F LPKT GK+LRR ++ Sbjct: 572 DKRLGSIGRPSHVYFVSALPKTRSGKLLRRSIQ 604 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.320 0.138 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,074,964 Number of extensions: 44798 Number of successful extensions: 109 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 3 Length of query: 566 Length of database: 1,000,347 Length adjustment: 87 Effective length of query: 479 Effective length of database: 713,682 Effective search space: 341853678 Effective search space used: 341853678 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)