BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3726.p (555 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA liga... 147 2e-36 gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Hermi... 118 6e-28 gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminii... 114 2e-26 >gi|134093504|ref|YP_001098579.1| long-chain-fatty-acid--CoA ligase [Herminiimonas arsenicoxydans] Length = 560 Score = 147 bits (371), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 137/520 (26%), Positives = 232/520 (44%), Gaps = 45/520 (8%) Query: 59 DRIICWQEINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNF 118 D+ + + E++ L L G++KG ++ + N Q + A + G V V N Sbjct: 46 DKFLTYAEVDTLSQKLGAWLQGTGLQKGARVALMMPNVLQYPVALAAVLRAGYVVVNVNP 105 Query: 119 RITPPEAAYLGQSSGACIMLYDRGFAHYVDAVRAASPTLQHVIAL--------------- 163 P E + SGA ++ FA ++ + S ++HV+ Sbjct: 106 LYKPRELQHQLVDSGAEAIIILENFATTLEKI-IVSTQIKHVVVATMGDMLGGLKGLLVN 164 Query: 164 -----------AEARPDELDYEALLAEGDGSPFAEAEVDHQDPLWFFYTSGTTGHPKAGV 212 A + P + ++ +LA+ + QD + YT GTTG K + Sbjct: 165 FVVRHVKKMVPAFSLPQAVSFKDVLAQAAHMHLQRPLLGPQDIAFLQYTGGTTGTAKGAM 224 Query: 213 LSHGQMAFVVTNHLADLMPGLNHQ----SRSLVVA-PLSHGAGVHA--LVNTARGAASIL 265 L+H + + + A + P L + + + V A PL H + A ++ T G +IL Sbjct: 225 LTHRNIVANILQNEAWIWPVLRKEPPDKTLNFVCALPLYHIFALTACCMLGTRLGVMNIL 284 Query: 266 LSSERLVVEEAWQLVEKYRVDNLFTVPTIVKLLVEDASVDRYDHASLKHVIYAGAPMYRA 325 + + R + +L+ KY+++ L V T+ L+ + + D + LK G + + Sbjct: 285 IPNPRDIPSLVKELM-KYKINLLPAVNTLYNGLLNNPDFAKVDFSELKLCNGGGMAVQQV 343 Query: 326 DQVYALKKLGRVLVQYYGLGEVTGNITFLPPYMHFEDDDHPQARVGTCGIPRTGMQIAIL 385 G +++ YGL E + T P E + GT G+P ++ IL Sbjct: 344 VNDRWRVVTGCSIIEGYGLSETSPVATSNP----VETTEFS----GTIGLPVPSTEVMIL 395 Query: 386 DDDGQSLPPRQTGEICVRGPAVFMGYHNNPEANAKAFKHD-WFHTGDLGHVDEEGFLYIT 444 D + + +GEI +RGP V GY NNP A+A D +F TGD+G +D G+ I Sbjct: 396 DSNDNEVALGLSGEIAIRGPQVMAGYWNNPAETAQAMTADGFFKTGDIGVMDARGYTRIV 455 Query: 445 GRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKPGQQVDE 504 R DM + G NVYP EIE + HP V E A +GVPD GE+ + + V + + Sbjct: 456 DRKKDMILVSGFNVYPSEIENVVAGHPGVLECACIGVPDEHTGEA-VKLFVVRRDPALTA 514 Query: 505 QALLAHLETRIGKYKWPRRFVYWETMPKSGYGKIVKKQIK 544 +AL+A + + YK P+ + +PKS GKI++++++ Sbjct: 515 EALMAFCKEQFTGYKKPKMIEFRTELPKSNVGKILRRELR 554 >gi|134096422|ref|YP_001101497.1| propionyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 634 Score = 118 bits (296), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 124/548 (22%), Positives = 236/548 (43%), Gaps = 56/548 (10%) Query: 45 EPGLILAQGHADGSDRIICWQEINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAW 104 +P LI D S+++ + +++A V+ A + GV +GD++L++ + + Sbjct: 73 QPALIAISTETD-SEKVYTFAQLHAEVNRCAAMMQSLGVSQGDRVLIYMPMVAEAAFAML 131 Query: 105 AAFKMGVV-WVPTNFRITPPEAAYLGQSSGACIMLYDRGF--AHYVDAVRAASPTLQHVI 161 A ++G + V + A + ++ I+ D G H V L I Sbjct: 132 ACARIGAIHSVVFGGFASNSLATRIDDATPVLILSADAGSRGCH----VMPYKSLLDEAI 187 Query: 162 ALAEARPD------------------ELDYEALLAEGDGSPFAEAEVDHQDPLWFFYTSG 203 LAE +P ++DY L + + ++ P + YTSG Sbjct: 188 VLAEHKPTHVLMVNRGLAPMQTVAGRDIDYATLREKHMDAAVPVVWLESNAPSYILYTSG 247 Query: 204 TTGHPKAGVLSHGQMAFVVTNHLADLMPGLNHQS---RSLVVAPLSHGAGVHALVNTARG 260 TTG PK V G A +T + + G ++ S + + H ++ + G Sbjct: 248 TTGKPKGVVRDVGGYAVALTTSMDLIFCGKAGETFFCTSDIGWVVGHSYIIYGPL--LAG 305 Query: 261 AASILLSSERLVVEEA--WQLVEKYRVDNLFTVPTIVKLLVED--ASVDRYDHASLKHVI 316 A++L + + + W +VEKY+V + + PT +++L + + ++D +SLK + Sbjct: 306 MATVLYEGLPIRPDASIWWSIVEKYKVTRMSSAPTAIRVLKKQPLELLKKHDLSSLKALY 365 Query: 317 YAGAPMYRADQVYALKKLGRVLVQYYGLGEVTGNITFLPPYMHFEDDDHPQARVGTCGIP 376 AG P+ + + L ++ Y E I + + D P R G+ G+P Sbjct: 366 LAGEPLDQTTSEWISGVLNVPVIDNYWQTETGWPIISIAKGV----GDKP-TRSGSPGLP 420 Query: 377 RTGMQIAILDD-DGQSLPPRQTGEICVRGP-------AVFMGYHNNPEANAKAFKHDWFH 428 G + I+++ G+ + G + + GP ++ + FK + + Sbjct: 421 LYGYKTKIINEISGEECAANEKGVVIIEGPLPPGCLQTLYGDDQRFVSTYWRNFKREAYS 480 Query: 429 TGDLGHVDEEGFLYITGRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGE 488 T D G D +G+ +I GR+ D+ G + REIEE++ +H VSEVAV+GV +P G+ Sbjct: 481 TFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHGNVSEVAVVGVENPIKGQ 540 Query: 489 SGLAVIVCKPGQQVD--------EQALLAHLETRIGKYKWPRRFVYWETMPKSGYGKIVK 540 +A ++ K E ++ ++ R+G P + +PK+ GK+++ Sbjct: 541 CAVAFVILKNAALAASPEEAKKLEAEIMKVVDKRLGSIGRPSHVYFVSALPKTRSGKLLR 600 Query: 541 KQIKALLE 548 + I+A+ E Sbjct: 601 RSIQAICE 608 >gi|134094157|ref|YP_001099232.1| acetyl-CoA synthetase [Herminiimonas arsenicoxydans] Length = 660 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 109/405 (26%), Positives = 185/405 (45%), Gaps = 43/405 (10%) Query: 177 LAEGDGSPFAEAEVDHQDPLWFFYTSGTTGHPKAGVLSHGQMAFVVTNHLA-----DLMP 231 L E S A V + PL+ YTSG+TG PK + H +++ L D+ P Sbjct: 246 LVEQRTSECAPEPVGAEHPLFILYTSGSTGKPKG--VQHSSAGYLLWAMLTMKWVFDIKP 303 Query: 232 GLNHQSRSLVVAPLSHGAGVHALVNTARGAASILLSSERLVVEEA--WQLVEKYRVDNLF 289 + + + H + + A GA I+ + W++V+K++V + Sbjct: 304 NDVYWCTADIGWVTGHTYVTYGPL--AVGATQIVFEGVPMYPNAGRFWEMVQKHKVSIFY 361 Query: 290 TVPTIVKLLVEDASVD------RYDHASLKHVIYAGAPMYRADQVYALKKLGRV---LVQ 340 T PT ++ L++ A D +YD +SL+ + G P+ ++ K +G +V Sbjct: 362 TAPTAIRSLIKAAEADPSTHPSKYDLSSLRLLGSVGEPINPEAWMWYYKNIGGERCPIVD 421 Query: 341 YYGLGEVTGN-ITFLPPYMHFEDDDHPQARVGTCGIPRTGMQIAILDDDGQSLPPRQTGE 399 + E G+ I+ LP G+C +P G+ A++DD G LP + G Sbjct: 422 TFWQTETGGHVISPLPGATPMVP--------GSCTLPLPGIIAAVVDDGGHDLPNGKGGL 473 Query: 400 ICVR--GPAVFMGYHNNPEANAKA-FKHDW----FHTGDLGHVDEE-GFLYITGRSSDMY 451 + ++ PA+ N+PE K+ F H+ + GD +EE G+ ITGR D+ Sbjct: 474 LVIKRPWPAMIRNIWNDPERFEKSYFPHELGGRTYLAGDGAIRNEETGYFTITGRIDDVL 533 Query: 452 ISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKPGQQVDEQALLAHL 511 G + EIE AL+ +P V+E AV+G PD GES A +V K + ++A Sbjct: 534 NISGHRMGTAEIESALVANPMVAEAAVVGKPDETTGESICAFVVLKRVRPEGDEAKQIAK 593 Query: 512 ETR------IGKYKWPRRFVYWETMPKSGYGKIVKKQIKALLEEQ 550 E R IG P+ + + +PK+ GKI+++ ++ L +++ Sbjct: 594 ELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKDE 638 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.319 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 1,132,890 Number of extensions: 48862 Number of successful extensions: 125 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 3 Length of query: 555 Length of database: 1,000,347 Length adjustment: 86 Effective length of query: 469 Effective length of database: 716,977 Effective search space: 336262213 Effective search space used: 336262213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)