BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3678.p (264 letters) Database: BlastDatabases/690/690 3295 sequences; 1,000,347 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/is... 100 7e-23 gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimo... 99 2e-22 gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimo... 87 8e-19 >gi|134095152|ref|YP_001100227.1| putative enoyl-CoA hydratase/isomerase family protein [Herminiimonas arsenicoxydans] Length = 301 Score = 100 bits (249), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 84/256 (32%), Positives = 128/256 (50%), Gaps = 13/256 (5%) Query: 11 RHDQRGITTLTLNRPQQFNALSEAMLQALQTQLDALAHDDQVRCVILAANGRAFCAGHDL 70 RH+ I LTL+ P NAL M A A DD +R VIL FCAG +L Sbjct: 51 RHEATLI--LTLSNPGAKNALHPDMFAAAIETFSTAARDDSIRAVILTGADNFFCAGGNL 108 Query: 71 R---EMHATPEQAYYQQLFERCSQLMQSLRALPVPVIAKVQGLATAAGCQLVASCDLAVA 127 E+ A ++A + ++++R P PVIA V+ A AG L +CDL VA Sbjct: 109 NTLLELRAQ-DRADQADAVDSLRGWIEAIRDCPKPVIAAVESAAAGAGFSLALACDLIVA 167 Query: 128 ADSARFAVSGINVGLFCSTP----AVALTRNLPIKRAFEMLVTGKFIDAATAADWGLINQ 183 +A+FA+S +N+GL TP + L++ LP + A E+++ GK + A D G+IN+ Sbjct: 168 GANAKFAMSYVNIGL---TPDGGASWFLSQALPRQLATEIMLEGKPVPAQRLHDLGVINR 224 Query: 184 AVPETQLDAATEALAREICSKPAISMARGKALVYQQQMMALPDAYAHAAEVMACNIMDGE 243 V + + A E+ + ++ R K+L + + +LP + ++ + Sbjct: 225 IVADGATLQTSLDWADELAALSGHAVERIKSLTREARDHSLPQHFEIEKNHFVDSLNHRD 284 Query: 244 AMEGIAAFLEKRAPQW 259 A EGI AFLEKRAP + Sbjct: 285 AQEGIHAFLEKRAPHY 300 >gi|134093495|ref|YP_001098570.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 258 Score = 99.0 bits (245), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 73/261 (27%), Positives = 125/261 (47%), Gaps = 15/261 (5%) Query: 8 LLLRHDQRGITTLTLNRPQQFNALSEAMLQALQTQLDALAHDDQVRCVILAANGRAFCAG 67 L+ H + G+ +TLNRP NAL++ ++ L L + ++ + C+I+ + +AF AG Sbjct: 7 LIEIHGKVGL--ITLNRPAALNALNDKLMDELGKALLSFDANEDIGCMIITGSEKAFAAG 64 Query: 68 HDLREMHATPEQAYYQQLFERCSQLMQSLRALPVPVIAKVQGLATAAGCQLVASCDLAVA 127 D+ M Y+ + ++ + +R++ PVIA V G A GC+L CD +A Sbjct: 65 ADIGAMATYSYMDVYKGDY--ITRNWEQIRSVRKPVIAAVAGYALGGGCELAMMCDFIIA 122 Query: 128 ADSARFAVSGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDAATAADWGLINQ 183 AD+A+F I +G+ P T+ LP +A +M +T + +DAA A GL+++ Sbjct: 123 ADTAKFGQPEIKLGII---PGAGGTQRLPRSVSKSKAMDMCLTARMMDAAEAERAGLVSR 179 Query: 184 AVPETQLDAATEALAREICSK--PAISMARGKALVYQQQMMALPDAYAHAAEVMACNIMD 241 V +L A A I S P + M K + + L + + Sbjct: 180 VVAADKLQEEALAAATVIASMSLPMVMMI--KESINRAYETTLAEGVQFERRLFHSTFAT 237 Query: 242 GEAMEGIAAFLEKRAPQWPST 262 + EG+ AF+EKRA ++ + Sbjct: 238 EDQKEGMRAFVEKRAAKFKNV 258 >gi|134095889|ref|YP_001100964.1| enoyl-CoA hydratase [Herminiimonas arsenicoxydans] Length = 264 Score = 87.0 bits (214), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 71/256 (27%), Positives = 114/256 (44%), Gaps = 15/256 (5%) Query: 14 QRGITTLTLNRPQQFNALSEAMLQALQTQLDALAHDDQVRCVIL-AANGRAFCAGHDL-- 70 Q GI + +RPQ NA++ AM L + + D +R A G AF AG D+ Sbjct: 17 QDGIAAILFDRPQARNAMTWAMYTELGAICERIRADQSIRVTTFRGAGGEAFVAGTDIEQ 76 Query: 71 -REMHATPEQAYYQQLF-ERCSQLMQSLRALPVPVIAKVQGLATAAGCQLVASCDLAVAA 128 R + + Y+++ ER Q+ ALP+P +A V G A G + A+CD +A Sbjct: 77 FRNFSSGEDGIAYERIVDERIGQI----EALPMPTLAIVDGWAIGGGLAIAAACDFRIAT 132 Query: 129 DSARFAVS-GINVGLFCSTPAVA-LTRNLPIKRAFEMLVTGKFIDAATAADWGLINQAVP 186 ++RF + +G S +A + + RA +L+ + + A G + + V Sbjct: 133 PASRFGIPIARTLGNCLSMANIARVVAAFGVGRAKRILLLAEVLAADELLAAGFVTEVVE 192 Query: 187 ETQLDAATEALAREICSKPAISMARGKALVYQQQMMALPDAYAHAAEVMACNIMDGEAME 246 LDA +AL +++ S I+M K + + LPD + AC + Sbjct: 193 AAVLDATADALCKKLASNAPITMGVSKEAIRRLTQAGLPDGEDL---IRAC-YGSSDFKI 248 Query: 247 GIAAFLEKRAPQWPST 262 G+ AFLEK+ P W Sbjct: 249 GVHAFLEKQRPLWSGN 264 Database: BlastDatabases/690/690 Posted date: Apr 9, 2011 4:48 AM Number of letters in database: 1,000,347 Number of sequences in database: 3295 Lambda K H 0.322 0.130 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 3295 Number of Hits to DB: 419,788 Number of extensions: 14031 Number of successful extensions: 66 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 4 Length of query: 264 Length of database: 1,000,347 Length adjustment: 80 Effective length of query: 184 Effective length of database: 736,747 Effective search space: 135561448 Effective search space used: 135561448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)